Review
Chemistry, Multidisciplinary
Xinyan Zhu, Xiaohan Wang, Hongxin Zhang, Fan Zhang
Summary: Luminescence lifetime imaging has advantages over traditional optical imaging in terms of precise quantification detection, and the long luminescence lifetime of lanthanide-doped nanoparticles (LnNPs) has attracted great attention. LnNPs with long luminescence lifetime not only make it easier to filter out background signals during imaging, but also provide a wide range of tunable lifetime. This article introduces the luminescence mechanisms of LnNPs, strategies for luminescence lifetime modulation, data acquisition methods, and cutting-edge applications in lifetime imaging systems, as well as prospects for improving technologies and extending applications of luminescence lifetime imaging.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Materials Science, Multidisciplinary
Dang Van Thai, Pham Van Ben, Vu Quoc Trung, Bui Hong Van, Nguyen Manh Nghia, Tran Minh Thi
Summary: ZnS:M and ZnS:Cu-PVP nanoparticles were synthesized using different methods and their photoluminescence intensity was enhanced by ultraviolet and visible radiation. The radiation annealing process induced photochemical and polymerization reactions on the surface of nanoparticles, resulting in improved optical properties.
Article
Multidisciplinary Sciences
Jin Jegal, Dongwoo Jeong, Eun-Suk Seo, HyeoungWoo Park, Hongjoo Kim
Summary: A novel hermetic detector composed of bismuth germanium oxide crystal scintillators and silicon photomultipliers has been developed for positronium annihilation studies. This detector is capable of detecting gamma-ray decay in all directions, allowing for the study of exotic decay processes and tumor localization. A convolutional neural network (CNN) is employed for Ps annihilation reconstruction and tumor localization.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Electrical & Electronic
Felipe Oliveira Barino, Eduardo Pestana de Aguiar, Leonardo de Mello Honorio, Vinicius Nunes Henrique Silva, Andres Pablo Lopez-Barbero, Alexandre Bessa dos Santos
Summary: This work reports on a new interrogation method for long-period fiber-grating (LPFG) sensors. The proposed method utilizes a multifilter LPFG interrogator for LPFG resonant wavelength detection and tracking. Results show that the new approach significantly reduces the uncertainty of the resonant wavelength compared to traditional methods.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2022)
Article
Multidisciplinary Sciences
Pawel Moskal, Kamil Dulski, Neha Chug, Catalina Curceanu, Eryk Czerwinski, Meysam Dadgar, Jan Gajewski, Aleksander Gajos, Grzegorz Grudzien, Beatrix C. Hiesmayr, Krzysztof Kacprzak, Lukasz Kaplon, Hanieh Karimi, Konrad Klimaszewski, Grzegorz Korcyl, Pawel Kowalski, Tomasz Kozik, Nikodem Krawczyk, Wojciech Krzemien, Ewelina Kubicz, Piotr Malczak, Szymon Niedzwiecki, Monika Pawlik-Niedzwiecka, Michal Pedziwiatr, Lech Raczynski, Juhi Raj, Antoni Rucinski, Sushil Sharma, Shivani, Roman Y. Shopa, Michal Silarski, Magdalena Skurzok, Ewa L. Stepien, Monika Szczepanek, Faranak Tayefi, Wojciech Wislicki
Summary: This study presents a new method for positronium imaging by simultaneous registration of annihilation photons and deexcitation photons from pharmaceuticals labeled with radionuclides, demonstrating the first positronium imaging of a model. It is anticipated that positronium imaging will substantially enhance the specificity of PET diagnostics.
Article
Optics
Dongrui Xiao, Liyang Shao, Chao Wang, Weihao Lin, Feihong Yu, Guoqing Wang, Tao Ye, Weizhi Wang, Mang Vai
Summary: A new design of an optical fiber sensor network interrogation platform is proposed based on nonuniformly spaced microwave photonic delay-line filter technology, which can convert sensing information from different types of optical sensors effectively. The platform allows simultaneous interrogation of multiple optical sensors via inverse Fourier transform, showing good sensitivity to variations such as twist, pressure, loading, and temperature. The presented approach provides a centralized solution for different types of optical sensors and can be easily expanded for larger sensor networks.
Article
Chemistry, Multidisciplinary
Zhenchao Liu, Tingbiao Guo, Qin Tan, Zhipeng Hu, Yuwei Sun, Houxin Fan, Zhi Zhang, Yi Jin, Sailing He
Summary: For the first time, an all-dielectric BIC metasurface is used for highly sensitive phase interrogation refractive index sensing. The proposed sensor achieves high performance and a high figure of merit, rivaling metal-based sensors. This scheme plays a pivotal role in the field of ultrahigh sensitivity sensors and opens possibilities for trace detection in biochemical analysis and environment monitoring.
Article
Chemistry, Multidisciplinary
Rania E. Morsi, Denis Gentili, Franco Corticelli, Vittorio Morandi, Alberto Figoli, Francesca Russo, Francesco Galiano, Giovanna Angela Gentilomi, Francesca Bonvicini, Ilse Manet, Barbara Ventura
Summary: The development of polymeric fabrics with photoinduced antibacterial activity is crucial for various applications. In this study, cellulose acetate membranes were prepared with the addition of additives, including a photosensitizer (TPP), a surfactant (Pluronic F-127), and graphene oxide (GO). The combination of these three additives exhibited excellent antibacterial properties.
Article
Engineering, Electrical & Electronic
Abhinav Gautam, Amitesh Kumar
Summary: This paper presents a fiber Bragg gratings (FBG)-based inclinometer with high sensitivity and accuracy. It utilizes an interrogation technique that employs edge filter detection and phase modulation to intensity modulation (PM-IM) conversion to achieve higher sensitivity and resolution.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2022)
Article
Chemistry, Analytical
Rajannya Sen, Liang Li, Alexander V. Zhdanov, Rafael D. L. Gaspar, Dmitri B. Papkovsky
Summary: This study evaluated four different detection platforms for phosphorescence lifetime (PLT) measurements. The results showed that the Victor 2 fluorescence reader produced stable but inaccurate readings at low PLT values, while the Cary Eclipse luminescence spectrometer was able to partly correct this issue. The Tpx3Cam camera-based macroscope and the confocal laser-scanning FLIM/PLIM microscope provided more accurate and informative PLT data.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Engineering, Electrical & Electronic
Aleksandr A. Markvart, Leonid B. Liokumovich, Iurii O. Medvedev, Nikolai A. Ushakov
Summary: This study reports the first smartphone-based interrogation of a fiber Bragg grating sensor, utilizing the smartphone flashlight LED and a transmissive diffraction grating. The measurement sensitivity and resolution were validated through comparison with a commercial spectrometer and theoretical results. Additionally, the influence of modal noise on the smartphone-based fiber optic sensor interrogation system performance was considered for the first time.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shivanand Pudakalakatti, Mark Titus, Jose S. Enriquez, Sumankalai Ramachandran, Niki M. Zacharias, Imad Shureiqi, Yi Liu, James C. Yao, Xiangsheng Zuo, Pratip K. Bhattacharya
Summary: Peroxisome proliferator-activated receptor delta (PPARD) plays an essential role in cell metabolism, cell proliferation, inflammation, and tumorigenesis. This study investigated the metabolic changes regulated by PPARD in gastric tumor development. The findings suggest that PPARD overexpression leads to alterations in metabolite levels, particularly in inosine monophosphate, uracil, phenylalanine, glycine, isocitrate, inosine, adenosine monophosphate, UDP-glucose, and oxypurinol. Additionally, fatty acid oxidation was identified as a potential bioenergy source for PPARD-expressing gastric tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Ulrich Nordmeyer, Torsten Thiel, Konstantin Kojucharow, Niels Neumann
Summary: Although microwave photonic approaches have been used for fiber sensing applications before, most previous contributions focused on evaluating the amplitude of the sensor signal. This paper proposes a scheme based on the phase response of a fiber Bragg grating for the interrogation of fiber sensors, and compares it with the amplitude-based approach. The findings show a significant improvement in the signal-to-noise ratio for the phase response evaluation, indicating the potential benefits of this approach for radio-over-fiber sensing systems.
Article
Chemistry, Inorganic & Nuclear
Anuj Dalal, Kapeesha Nehra, Anjli Hooda, Sitender Singh, Shri Bhagwan, Devender Singh, Sumit Kumar
Summary: This study reports the synthesis and photoluminescence performance of a series of heteroleptic europium complexes with fluorinated chromophoric ligand and 2,2'-bipyridine or their derivatives as ancillary ligand. The structure and photoluminescence properties of the synthesized complexes were analyzed using various spectroscopic techniques. The experimental and theoretical results from LUMPAC software show that the prepared complexes exhibit red emissive nature and can be used as red light emitting materials in various display devices.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Engineering, Electrical & Electronic
Jianxiang Zhang, Wenzhu Huang, Wentao Zhang, Fang Li
Summary: This study introduces an improved method that combines the 3x3 coupler and extended Kalman filter to effectively suppress harmonic distortion in distributed feedback fiber laser sensor interrogation. The method can update system parameters in real-time, leading to a low total harmonic distortion and large dynamic range in signal frequency range of 20 Hz to 1 kHz.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2021)
Article
Cell Biology
Irina Druzhkova, Maria Lukina, Varvara Dudenkova, Nadezhda Ignatova, Ludmila Snopova, Alena Gavrina, Lyubov Shimolina, Elena Zagaynova, Marina Shirmanova
Summary: Genetically encoded pH-sensors are useful for monitoring intracellular pH changes, especially in the context of cancer and chemotherapy response. Studies using genetically encoded ratiometric pH-sensor SypHer2 reveal dynamic changes in pH levels in tumor cells in response to taxol treatment, shedding light on the mechanisms of taxol action on cervical cancer cells and offering potential for new approaches to cancer treatment.
Article
Biophysics
Liubov Shimolina, Ekaterina Potekhina, Irina Druzhkova, Maria Lukina, Varvara Dudenkova, Vsevolod Belousov, Vladislav Shcheslavskiy, Elena Zagaynova, Marina Shirmanova
Summary: Changes in intracellular pH reflect the metabolic states of cancer cells during tumor growth and dissemination. Monitoring pH is essential for understanding the metabolic mechanisms supporting cancer progression. Genetically encoded fluorescent pH sensors provide real-time tracking of pH in specific subcellular compartments, but their ratiometric readout is not suitable for thick samples ex vivo or tissues in vivo, including solid tumors. Fluorescence lifetime imaging (FLIM) offers a promising alternative to conventional fluorescence microscopy. This study presents a quantitative approach to map pH in cancer cells and tumors in vivo using the fluorescence lifetime of a genetically encoded pH sensor called SypHerRed. The study demonstrates the utility of SypHerRed in visualizing pH in cancer cell culture and mouse tumor xenografts using FLIM and macroscopy. For the first time, the absolute pH value is obtained for tumors in vivo using an optical technique. Additionally, the study shows the simultaneous detection of pH and the endogenous fluorescence of metabolic cofactor NADH, providing complementary insights into the metabolic aspects of cancer. The fluorescence lifetime-based readout and red-shifted spectra of SypHerRed make it a promising instrument for multiparameter in vivo imaging applications.
BIOPHYSICAL JOURNAL
(2022)
Article
Oncology
Ilya Turchin, Shazia Bano, Mikhail Kirillin, Anna Orlova, Valeriya Perekatova, Vladimir Plekhanov, Ekaterina Sergeeva, Daria Kurakina, Aleksandr Khilov, Alexey Kurnikov, Pavel Subochev, Marina Shirmanova, Anastasiya Komarova, Diana Yuzhakova, Alena Gavrina, Srivalleesha Mallidi, Tayyaba Hasan
Summary: A new approach of combined optoacoustic and fluorescence imaging was developed to trace photosensitizer kinetics and vascular effects in vivo. The study demonstrated the enhancement of photosensitizer accumulation in tumor and alterations in tumor vasculature structure after treatment. The results suggest that the multifunctional liposomal platform combined with multimodal imaging offers an efficient and personalized platform to improve cancer treatment outcomes.
Article
Optics
Anna Orlova, Yulia Perevalova, Ksenia Pavlova, Natalia Orlinskaya, Aleksandr Khilov, Daria Kurakina, Maria Shakhova, Mikhail Kleshnin, Ekaterina Sergeeva, Ilya Turchin, Mikhail Kirillin
Summary: In this study, the oxygenation dynamics in experimental tumors after PDT treatment with chlorin-based photosensitizers using red or blue light were monitored. The results showed that red light PDT led to a gradual decrease in tumor oxygen saturation due to blood flow arrest, while blue light PDT maintained the blood oxygen saturation in the tumor.
Article
Multidisciplinary Sciences
Evgeny A. Shirshin, Marina V. Shirmanova, Alexey V. Gayer, Maria M. Lukina, Elena E. Nikonova, Boris P. Yakimov, Gleb S. Budylin, Varvara V. Dudenkova, Nadezhda I. Ignatova, Dmitry V. Komarov, Vladislav V. Yakovlev, Wolfgang Becker, Elena V. Zagaynova, Vladislav I. Shcheslavskiy, Marlan O. Scully
Summary: The heterogeneity of cells determines their response to external factors, and the metabolic heterogeneity of tumor cells is of clinical relevance. Fluorescence lifetime imaging (FLIM) is a non-invasive technique that can sense and quantify cellular metabolism by measuring the fluorescence decay parameters of endogenous fluorophores. This study demonstrates the unique capability of FLIM in accurately discriminating metabolic heterogeneity using a non-parametric analysis approach, which has been validated in cultured cancer cells treated with chemotherapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Marina Shirmanova, Alena Gavrina, Tatiana F. Kovaleva, Varvara V. Dudenkova, Ekaterina E. Zelenova, Vladislav Shcheslavskiy, Artem M. Mozherov, Ludmila B. Snopova, Konstantin A. Lukyanov, Elena Zagaynova
Summary: Cellular redox status and ROS level are closely related to the apoptotic potential of cancer cells. This study investigates the correlation between ROS production and redox cofactors using fluorescence imaging. The results suggest new therapeutic strategies based on cellular redox balance regulation to improve anti-tumor activity and overcome apoptotic resistance.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Anna Orlova, Ksenia Pavlova, Aleksey Kurnikov, Anna Maslennikova, Marina Myagcheva, Evgeniy Zakharov, Dmitry Skamnitskiy, Valeria Perekatova, Alexander Khilov, Andrey Kovalchuk, Alexander Moiseev, Ilya Turchin, Daniel Razansky, Pavel Subochev
Summary: This study demonstrates the size-dependent nature of vascular responses to radiation treatments at varying doses in vivo using scanning optoacoustic (OA) microangiography. The OA approach enables the study of radiation-induced vascular changes in an unperturbed in vivo environment while allowing for high-resolution observations of deep tissue at the whole tumor scale.
Article
Multidisciplinary Sciences
Anton Barchuk, Dmitriy Skougarevskiy, Alexei Kouprianov, Daniil Shirokov, Olga Dudkina, Rustam Tursun-Zade, Mariia Sergeeva, Varvara Tychkova, Andrey Komissarov, Alena Zheltukhina, Dmitry Lioznov, Artur Isaev, Ekaterina Pomerantseva, Svetlana Zhikrivetskaya, Yana Sofronova, Konstantin Blagodatskikh, Kirill Titaev, Lubov Barabanova, Daria Danilenko
Summary: The COVID-19 pandemic in Russia has caused over 500,000 excess deaths and more than 5.6 million cases by July 2021. Surveillance based on case reporting is the core monitoring method. Seroprevalence studies offer unbiased estimates of disease spread, combining with other surveillance tools to define pandemic course.
Article
Biochemistry & Molecular Biology
Yulia P. Parshina, Anastasia D. Komarova, Leonid N. Bochkarev, Tatyana A. Kovylina, Anton A. Plekhanov, Larisa G. Klapshina, Aleksey N. Konev, Artem M. Mozherov, Ilya D. Shchechkin, Marina A. Sirotkina, Vladislav Shcheslavskiy, Marina Shirmanova
Summary: Tumor cells are able to grow in conditions of variable oxygen supply and hypoxia by switching between different metabolic pathways. This study develops a methodology for analyzing the metabolic status and oxygen level of tumor cells simultaneously. The results reveal a high degree of heterogeneity in both metabolic and oxygen states of mouse tumors, with a lower dependence of metabolism on oxygen compared to in vitro conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Anastasia N. N. Kazakova, Ksenia S. S. Anufrieva, Olga M. M. Ivanova, Polina V. V. Shnaider, Irina K. K. Malyants, Olga I. I. Aleshikova, Andrey V. V. Slonov, Lev A. A. Ashrafyan, Nataliya A. A. Babaeva, Artem V. V. Eremeev, Veronika S. S. Boichenko, Maria M. M. Lukina, Maria A. A. Lagarkova, Vadim M. M. Govorun, Victoria O. O. Shender, Georgij P. P. Arapidi
Summary: This study summarized a large number of single-cell RNA-sequencing data from various tumor types and normal tissues to identify marker genes and protein markers for cancer-associated fibroblasts (CAFs). The findings revealed common differences in gene expression patterns between normal and cancer-associated fibroblasts, independent of tumor type.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Olga M. Ivanova, Ksenia S. Anufrieva, Anastasia N. Kazakova, Irina K. Malyants, Polina V. Shnaider, Maria M. Lukina, Victoria O. Shender
Summary: Dysregulation of pre-mRNA splicing is a common characteristic of cancer cells and is linked to changes in expression, localization, and mutations of splicing machinery components. Recent studies have revealed that spliceosome components can also affect cellular processes independently of splicing. This review analyzes open source data and literature relating to the impact of splicing factor knockdown on gene expression and splicing in human cells, particularly regarding cell proliferation, migration, cell cycle regulation, DNA repair, and cell death. The review also explores the involvement of splicing factors in intercellular communication and autoregulatory mechanisms, as well as potential strategies for targeting spliceosome components in anticancer therapy.
CELL DEATH & DISEASE
(2023)
Article
Chemistry, Multidisciplinary
Nataliia A. Petrunina, Alina S. Shtork, Maria M. Lukina, Vladimir B. Tsvetkov, Yuri M. Khodarovich, Alexey V. Feofanov, Anastasia M. Moysenovich, Eugene G. Maksimov, Victoria O. Shipunova, Timofei S. Zatsepin, Alexandra N. Bogomazova, Victoria O. Shender, Andrey V. Aralov, Maria A. Lagarkova, Anna M. Varizhuk
Summary: DNA-intercalated motifs serve as convenient scaffolds for designing pH-responsive nanomachines, including biocompatible pH sensors. By using a simple unimolecular dual-labeled iM scaffold and modifying it with abasic or alkyl linkers, the thermal stability and pH-induced conformational transitions of the iM were improved.
Article
Medicine, Research & Experimental
D. V. Yuzhakova, M. M. Lukina, D. A. Sachkova, G. M. Yusubalieva, V. P. Baklaushev, A. M. Mozherov, V. V. Dudenkova, A. I. Gavrina, K. S. Yashin, M. V. Shirmanova
Summary: Patient-specific in vitro tumor models are a promising platform for studying the mechanisms of oncogenesis and personalized selection of drugs, especially for glial brain tumors with unsatisfactory treatment effectiveness. This study developed a model of a 3D tumor glioblastoma spheroid based on a patient's surgical material and studied its metabolic characteristics using fluorescence lifetime imaging microscopy of metabolic coenzymes.
SOVREMENNYE TEHNOLOGII V MEDICINE
(2023)
Article
Optics
Vladislav I. Shcheslavskiy, Diana V. Yuzhakova, Daria A. Sachkova, Marina Shirmanova, Wolfgang Becker
Summary: This study presents a laser scanning system for macroscopic samples that can record fully resolved decay curves and images at multiple laser wavelengths. The system utilizes confocal detection to eliminate lateral scattering and out-of-focus haze. The system's performance is demonstrated on model experiments and a tumor model in living mice.
