Journal
OPTICS EXPRESS
Volume 25, Issue 12, Pages 13668-13683Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.25.013668
Keywords
-
Categories
Funding
- California Institute of Regenerative Medicine (CIRM) [LA1-08013]
- National Institutes of Health (NIH) [UO1-EB021236, U54-DK107980]
- Region Ile de France (DIM Malinf)
- Visiting scholarship from the Siebel Stem Cell Foundation
- Institut Pasteur
Ask authors/readers for more resources
Three-dimensional fluorescence microscopy based on Nyquist sampling of focal planes faces harsh trade-offs between acquisition time, light exposure, and signal-to-noise. We propose a 3D compressed sensing approach that uses temporal modulation of the excitation intensity during axial stage sweeping and can be adapted to fluorescence microscopes without hardware modification. We describe implementations on a lattice light sheet microscope and an epifluorescence microscope, and show that images of heads and biological samples can be reconstructed with a 5-10 fold reduction of light exposure and acquisition time. Our scheme opens a new door towards faster and less damaging 3D fluorescence iriicroscopy. (C) 2017 Optical Society of America
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available