Article
Biochemistry & Molecular Biology
Elena Pires, Neelam Sharma, Platon Selemenakis, Bo Wu, Yuxin Huang, Dauren S. Alimbetov, Weixing Zhao, Claudia Wiese
Summary: RAD51AP1 is a key protein in the HR DNA repair pathway, which can bind to nucleosome core particles and assist in RAD51-mediated DNA capture and joint-molecule formation. This study reveals the mechanism of RAD51AP1 in chromatin and its role in DNA repair.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Oncology
Sandra van Wilpe, Donjete Simnica, Peter Slootbeek, Thomas van Ee, Samhita Pamidimarri Naga, Mark A. J. Gorris, Lieke L. van der Woude, Shabaz Sultan, Rutger H. T. Koornstra, Inge M. van Oort, Winald R. Gerritsen, Leonie Kroeze, Michiel Simons, Geert J. L. H. van Leenders, Mascha Binder, I. Jolanda M. de Vries, Niven Mehra
Summary: In this study, the tumor immune landscape and peripheral T cell receptor repertoire of patients with and without HRD-PCa were compared. The results showed that patients with HRD-PCa displayed increased tumor-infiltrating lymphocyte density and altered peripheral T cell receptor repertoire. Furthermore, it was suggested that immune checkpoint inhibitors might have enhanced efficacy in HRD-PCa.
Article
Biochemistry & Molecular Biology
Maram Essawy, Lisa Chesner, Duha Alshareef, Shaofei Ji, Natalia Tretyakova, Colin Campbell
Summary: This study reveals the mechanisms of how cells recognize DNA-protein crosslink lesions and selectively target them to nucleotide excision repair and recombinational DNA repair pathways through transfection experiments.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Mi Ae Kang, Jong-Soo Lee
Summary: CTCF, a highly conserved multifunctional DNA-binding protein with 11 zinc fingers, plays crucial roles in diverse genomic processes such as transcriptional regulation, insulation, genome imprinting, and maintenance of genome organization. Recent findings reveal that CTCF is involved in DNA double-strand break (DSB) repair through homologous recombination (HR), facilitating accurate restoration of broken DNA sequences. Understanding the functional crosstalks between CTCF and other HR factors may shed light on the molecular basis of various human diseases and the diverse functions of CTCF in genome biology.
Article
Oncology
Junru Chen, Yanfeng Tang, Huanhuan Liu, Guangxi Sun, Haoyang Liu, Junjie Zhao, Zilin Wang, Yanrui Zhang, Feng Lou, Shanbo Cao, Jiayue Qin, Huina Wang, Banghua Liao, Hao Zeng
Summary: This study reveals the genetic mutation profile of Chinese urothelial carcinoma patients and highlights the molecular rationale for utilizing immunotherapy in UC patients with HRR mutations.
Review
Oncology
Alexander M. A. van der Wiel, Lesley Schuitmaker, Ying Cong, Jan Theys, Arne Van Hoeck, Conchita Vens, Philippe Lambin, Ala Yaromina, Ludwig J. Dubois
Summary: This review discusses more accurate methods to detect homologous recombination deficiency (HRD) and how these can be applied for more personalized cancer treatment. Current methods to assess HRD are insufficient, leading to an underestimation of patients with HRD. Detection of HRD scar might be a more reliable biomarker for HRD.
Article
Chemistry, Multidisciplinary
Yuxuan Xiong, Wei Wang, Qingyuan Deng, Zhijie Zhang, Qiang Wang, Zhengtao Yong, Chaoyang Sun, Xiangliang Yang, Zifu Li
Summary: Various PARPi have been approved for the treatment of breast cancers, but they are only effective for HR-deficient breast cancers. The abnormal micro-environment of breast cancers hinders drug delivery. This study used mesoporous polydopamine to deliver PARPi and a DNA-damaging drug, and demonstrated that local mild photothermal therapy could inhibit the HR repair pathway and repress cancer-associated fibroblasts, thereby enhancing the antitumor effect and improving drug delivery.
Article
Biochemistry & Molecular Biology
Ricardo Peraza-Vega, Mahara Valverde, Emilio Rojas
Summary: The repair of DNA damage is crucial for maintaining genetic information and cell functioning. Double-strand breaks (DSBs) are the most harmful type of DNA damage, and their repair involves two main mechanisms: non-homologous end joining (NHEJ) and homologous recombination repair (HRR). MiRNAs have been shown to play an important role in regulating genes involved in NHEJ and HRR, and alterations in miRNA expression can impact the ability of cells to repair DSBs and affect cancer therapy sensitivity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Health Care Sciences & Services
Laura Cortesi, Claudia Piombino, Angela Toss
Summary: This article explores the role of germline mutations in HRR-related genes other than BRCA1/2 in predicting responses to treatment and prognosis in breast and pancreatic cancer. Mutations in PALB2 and ATM genes have shown potential as predictive factors for treatment response and prognosis, suggesting their inclusion in routine sequencing for biological characterization of these cancers.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
John J. Skoko, Juxiang Cao, David Gaboriau, Myriam Attar, Alparslan Asan, Lisa Hong, Candice E. Paulsen, Hongqiang Ma, Yang Liu, Hanzhi Wu, Trey Harkness, Cristina M. Furdui, Yefim Manevich, Ciaran G. Morrison, Erika T. Brown, Daniel Normolle, Maria Spies, Michael Ashley Spies, Kate Carroll, Carola A. Neumann
Summary: This study identifies RAD51 Cys319 as a functionally significant site for the redox regulation of homologous recombination (HR) and cellular responses to ionizing radiation (IR). The loss of peroxiredoxin 1 (PRDX1) inhibits RAD51 focus formation and HR DNA repair, leading to increased DNA damage and sensitization of cells to irradiation.
Review
Cell Biology
Thais Sobanski, Maddison Rose, Amila Suraweera, Kenneth O'Byrne, Derek J. Richard, Emma Bolderson
Summary: DNA repair and metabolic pathways are crucial for maintaining cellular homeostasis in normal human cells, but undergo significant changes during tumorigenesis. They are interdependent, and potential therapeutic targets in cancer treatment. More research into their links may open up new avenues for cancer treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaofei Liu, Yiwei Cao, Guijie Du, Chao Zhang, Meng Xu, Zhukuan Cheng, Yi Shen, Hengxiu Yu
Summary: The mechanisms of OsRAD51 and OsDMC1 in rice meiosis are still unknown. In this study, mutant plants for Osrad51a1, Osrad51a2, Osdmc1a, and Osdmc1b were obtained through CRISPR/Cas9, and it was found that OsRAD51A1 and OsRAD51A2 are functionally redundant in rice fertility. The study also suggests that OsRAD51 and OsDMC1 may functionally depend on each other and play important roles in meiotic recombination during meiosis in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Mamoru Ito, Makoto Kubo, Hitomi Kawaji, Yoshiki Otsubo, Kanako Kurata, Hikaru Abutani, Mikita Suyama, Yoshinao Oda, Tomoharu Yoshizumi, Masafumi Nakamura, Eishi Baba
Summary: This study found a positive association between HRR gene alterations and TMB-high as well as a good response to immunotherapy. It also highlighted the importance of avoiding samples with lower percentages of tumor cell nuclei and previous neoadjuvant chemotherapy for successful CGP.
Article
Urology & Nephrology
Konrad H. Stopsack
Summary: The PROfound trial found that treatment with the PARP inhibitor olaparib benefits men with metastatic castration-resistant prostate cancer harboring alterations in 15 different DNA repair genes. However, the benefit of olaparib was less significant, if present at all, for men with prostate cancer harboring non-BRCA DNA repair alterations compared to those with BRCA alterations.
Article
Multidisciplinary Sciences
Nanda Kumar Jegadesan, Dana Branzei
Summary: In cancer treatment, DDX11 plays a crucial role in regulating DNA repair mechanisms, particularly affecting sensitivity and resistance to chemotherapy drugs. Its mechanism of action includes assisting double strand break resection and loading RAD51 on single-stranded DNA to promote homologous recombination repair.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)