Article
Biochemical Research Methods
Mohamed Amgad, LameesA Atteya, Hagar Hussein, Kareem Hosny Mohammed, Ehab Hafiz, Maha A. T. Elsebaie, Pooya Mobadersany, David Manthey, David A. Gutman, Habiba Elfandy, Lee A. D. Cooper
Summary: This article presents techniques for scalable nuclear detection, segmentation, and explainable classification to realize the potential of computational pathology in computer-aided diagnosis and discovery of morphologic biomarkers. The modifications to the Mask R-CNN architecture and the introduction of the DTALE explainability method contribute to improving accuracy and providing simple, quantitative explanations for classification models, addressing the need for explainability in deep learning models.
Article
Virology
Paul Schmidle, Sonja Leson, Ulrike Wieland, Almut Boeer-Auer, Dieter Metze, Stephan A. Braun
Summary: Monkeypox is a rare viral zoonosis that has gained public attention since a major outbreak in May 2022. This study analyzed skin biopsies from four patients at different stages of the disease and found that the histological findings of monkeypox vary depending on the stage of the lesion. The early macular stage may be missed histologically, while immunohistochemistry with anti-Vaccinia virus antibodies can help distinguish monkeypox from other infections in later stages.
Article
Medicine, Research & Experimental
Diana D. Vucevic, Michael A. Seidman, Kumi Mesaki, Zehong Guan, Stephen Juvet, Mingyao Liu, Shaf Keshavjee, Kieran Murphy
Summary: In this study, a novel tissue preservation medium called Amber was developed and compared with formalin. It was found that Amber demonstrated superior performance in histology, immunohistochemistry, immunofluorescence, and RNA extraction, making it a safer and superior substitute for clinical tissue preservation.
LABORATORY INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Anca-Maria Istrate-Ofiteru, Elena-Iuliana-Anamaria Berbecaru, George-Lucian Zorila, Gabriela-Camelia Rosu, Laurentiu Mihai Dira, Cristina Maria Comanescu, Roxana Cristina Dragusin, Dan Ruican, Rodica Daniela Nagy, Dominic Gabriel Iliescu, Laurentiu Mogoanta, Daniel Pirici
Summary: This prospective study aims to explore the progression and development mechanisms of ectopic endometrial epithelium and found that inflammatory, vascular, and hormonal mechanisms may trigger the progression and neoplastic changes of endometriosis, increasing local pain. These mechanisms may represent future therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Ian A. Rowe, Richard Parker
Summary: This study quantified the contribution of placebo to liver fibrosis improvement in nonalcoholic steatohepatitis (NASH) patients through probabilistic simulation. The results showed that approximately 22% of NASH patients with fibrosis stage 2 or 3 at baseline improved by at least 1 fibrosis stage after receiving placebo treatment. The placebo response rate ranged from 15% to 30%, and an increase in observer variability further increased the placebo response.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Chemistry, Analytical
Alan M. Race, Daniel Sutton, Gregory Hamm, Gareth Maglennon, Jennifer P. Morton, Nicole Strittmatter, Andrew Campbell, Owen J. Sansom, Yinhai Wang, Simon T. Barry, Zoltan Takats, Richard J. A. Goodwin, Josephine Bunch
Summary: The article introduces a generic method for extracting information from different modalities using annotations from one microscopy modality and presents a fast, general multimodal registration workflow for automatic alignment of complex data sets. Experimental results demonstrate that the proposed methods help better understand tumor heterogeneity and the tumor microenvironment.
ANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Jure Krasic, Lucija Skara Abramovic, Marta Himelreich Peric, Vedran Vanjorek, Marko Gangur, Dragana Zovko, Marina Malnar, Silvija Masic, Alma Demirovic, Bernardica Juric, Monika Ulamec, Marijana Coric, Davor Jezek, Tomislav Kulis, Nino Sincic
Summary: This study compared data from the HPA with in-house immunohistochemistry on TGCT diagnostic genes and discovered discrepancies in three of the six core diagnostic genes. Additionally, DPPA3, CALCA, and TDGF1 were identified as potential novel biomarkers for TGCT.
Article
Oncology
Evan H. Einstein, Faina Ablyazova, Ashley Rosenberg, Manju Harshan, Samuel Wahl, Gady Har-El, Peter D. Constantino, Jason A. Ellis, John A. Boockvar, David J. Langer, Randy S. D'Amico
Summary: This study demonstrates that stimulated Raman histology (SRH) is non-inferior to frozen sectioning in terms of diagnostic capability and the quality and condition of tissue after analysis. It is the first study to provide evidence of SRH accuracy using one-to-one tissue analysis in neuropathological specimens.
JOURNAL OF NEURO-ONCOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Sergio Vazquez, Raquel Vallejo, Jose Espinosa, Noive Arteche, Jose A. Vega, Valentin Perez
Summary: This study investigated the relationship between macrophage infiltrates and histological grade and immunophenotype in cases of canine lymphoma. Results showed that high-grade and B-cell lymphomas had the highest number of macrophages, with a predominant M2 phenotype. The findings suggest a complex interaction between macrophages, tumor behavior, and immune response in canine lymphoma.
Article
Medicine, General & Internal
Ali Al Khader, Hadeel Odeh, Muna A. Salameh, Ezidin G. Kaddumi, Mohamad Al-Saghbini, Fatima N. Obeidat, Nisreen Abu Shahin, Nidal A. Younes
Summary: The study found that comparison-based teaching methods in practical histology can significantly improve overall examination scores for both first-year and second-year medical students. Students also showed a clear preference for the comparison-based approach over traditional methods.
Article
Veterinary Sciences
Kristiane Barington, Esben Ostergaard Eriksen, Egle Kudirkiene, Karen Pankoke, Katrine Top Hartmann, Mette Sif Hansen, Henrik Elvang Jensen, Sophie Amalie Blirup-Plum, Benjamin Meyer Jorgensen, Jens Peter Nielsen, John Elmerdahl Olsen, Nicole Bakkegard Goecke, Lars Erik Larsen, Ken Steen Pedersen
Summary: This study investigated gross and histological lesions and related pathogens in pigs that died during the nursery period in five Danish farms. It was found that lesions were most prevalent in the skin, respiratory system, gastrointestinal tract, and joints. Histopathological assessment should be combined with gross assessment to accurately diagnose lung and joint lesions, and in situ identification is needed to confirm the presence of pathogens.
PORCINE HEALTH MANAGEMENT
(2023)
Article
Agriculture, Dairy & Animal Science
Violaine Mechin, Patrick Pageat, Eva Teruel, Pietro Asproni
Summary: Chemical communication, specifically through the vomeronasal organ, plays a crucial role in animal behavior. This study demonstrates that aging significantly impacts the condition of the vomeronasal organ, leading to neuronal degeneration and changes in protein expression. The findings shed light on the effects of aging on chemical communication efficiency in animals.
Review
Radiology, Nuclear Medicine & Medical Imaging
Wadha Alyami, Andre Kyme, Roger Bourne
Summary: Validating MRI techniques for tissue characterization and disease-related tissue alterations requires rigorous validation with ground truth information such as histology. Direct correlation methods between histology and MRI often fail due to spatial errors. Standard protocols for clinical tissue processing and histology-based pathology reporting can be modified to address some of these errors, without compromising the clinical process. This review summarizes the advantages and limitations of MRI validation techniques based on coregistration of MRI with whole-mount histology of tissue specimens.
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2022)
Article
Veterinary Sciences
Francieli Adriane Molossi, Bruno Albuquerque de Almeida, Bianca Santana de Cecco, Caroline Pissetti, Lauren Ventura, Luciano Brandalise, Gustavo Simao, Fabio Vanucci, Tatiane Terumi Negrao Watababe, Itabajara da Silva Vaz Jr, David Driemeier
Summary: This study aimed to evaluate the relationship between PCV3 viral load and histopathological findings in perinatal piglet tissues and develop an immunohistochemical method for detecting the virus in lesions. The quantitative PCR Ct value and the area of perivascular inflammatory infiltrates were compared. PCV3 immunohistochemistry revealed granular immunolabeling in various organs. Ct values showed no significant difference between tissues, except for lymphoid organs. There was no correlation between Ct values and perivascular inflammatory infiltrates.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Ahmed A. J. Jabbar, Zaenah Zuhair Alamri, Mahmood Ameen Abdulla, Ahmed S. AlRashdi, Soran Kayfi Najmaldin, Mustafa AbdulMonam Zainel
Summary: Sinapic acid (SA) has protective effects against thioacetamide (TAA) induced fibrosis in rats by reducing hepatocyte injuries, inhibiting fibrosis, promoting heat shock protein (HSP) up-regulation, down-regulating alpha-smooth muscle actin (a-SMA) expression, and improving liver index and antioxidant enzymes levels.
Article
Endocrinology & Metabolism
James F. Powers, Karel Pacak, Arthur S. Tischler
ENDOCRINE PATHOLOGY
(2017)
Review
Cell Biology
John Turchini, Veronica K. Y. Cheung, Arthur S. Tischler, Ronald R. De Krijger, Anthony J. Gill
Article
Obstetrics & Gynecology
Alexa Calero, Hazel Villanueva, Priyadarshani Giri, Arthur S. Tischler, James F. Powers, Marian Evinger
AMERICAN JOURNAL OF PERINATOLOGY
(2018)
Editorial Material
Cell Biology
Katrin Huber, Isabelle Janoueix-Lerosey, Wolfgang Kummer, Hermann Rohrer, Arthur S. Tischler
CELL AND TISSUE RESEARCH
(2018)
Article
Oncology
James F. Powers, Brent Cochran, James D. Baleja, Hadley D. Sikes, Xue Zhang, Inna Lomakin, Troy Langford, Kassi Taylor Stein, Arthur S. Tischler
ENDOCRINE-RELATED CANCER
(2018)
Review
Oncology
Hartmut P. Neumann, William F. Young, Tobias Krauss, Jean-Pierre Bayley, Francesca Schiavi, Giuseppe Opocher, Carsten C. Boedeker, Amit Tirosh, Frederic Castinetti, Juri Ruf, Dmitry Beltsevich, Martin Walz, Harald-Thomas Groeben, Ernst von Dobschuetz, Oliver Gimm, Nelson Wohllk, Marija Pfeifer, Delmar M. Lourenco, Mariola Peczkowska, Attila Patocs, Joanne Ngeow, Ozer Makay, Nalini S. Shah, Arthur Tischler, Helena Leijon, Gianmaria Pennelli, Karina Villar Gomez de las Heras, Thera P. Links, Birke Bausch, Charis Eng
ENDOCRINE-RELATED CANCER
(2018)
Editorial Material
Pathology
Ricardo V. Lloyd, Sylvia L. Asa, Virginia A. LiVolsi, Peter M. Sadow, Arthur S. Tischler, Ronald A. Ghossein, R. Michael Tuttle, Yuri E. Nikiforov
Article
Clinical Neurology
Yuanxin Liang, Robert S. Heller, Julian K. Wu, Carl B. Heilman, Arthur S. Tischler, Knarik Arkun
JOURNAL OF NEUROLOGICAL SURGERY PART B-SKULL BASE
(2019)
Article
Endocrinology & Metabolism
Nada A. Farhat, James F. Powers, Annette Shepard-Barry, Patricia Dahia, Karel Pacak, Arthur S. Tischler
ENDOCRINE PATHOLOGY
(2019)
Article
Pathology
Lester D. R. Thompson, Anthony J. Gill, Sylvia L. Asa, Roderick J. Clifton-Bligh, Ronald R. de Krijger, Noriko Kimura, Paul Komminoth, Ernest E. Lack, Jacques W. M. Lenders, Ricardo V. Lloyd, Thomas G. Papathomas, Peter M. Sadow, Arthur S. Tischler
Summary: ICCR is a non-profit organization aiming to develop internationally agreed-upon standardized data sets for pathology tumor classification, staging, and reporting elements. The creation of a data set for pheochromocytoma and paraganglioma aims to provide more meaningful outcomes and management data.
Article
Oncology
Paal W. Wallace, Catleen Conrad, Sascha Brueckmann, Ying Pang, Eduardo Caleiras, Masanori Murakami, Esther Korpershoek, Zhengping Zhuang, Elena Rapizzi, Matthias Kroiss, Volker Gudziol, Henri J. L. M. Timmers, Massimo Mannelli, Jens Pietzsch, Felix Beuschlein, Karel Pacak, Mercedes Robledo, Barbara Klink, Mirko Peitzsch, Anthony J. Gill, Arthur S. Tischler, Ronald R. de Krijger, Thomas Papathomas, Daniela Aust, Graeme Eisenhofer, Susan Richter
JOURNAL OF PATHOLOGY
(2020)
Letter
Endocrinology & Metabolism
James F. Powers, Arthur S. Tischler
ENDOCRINE PATHOLOGY
(2020)
Article
Oncology
James F. Powers, Brent Cochran, James D. Baleja, Hadley D. Sikes, Andrew D. Pattison, Xue Zhang, Inna Lomakin, Annette Shepard-Barry, Karel Pacak, Sun Jin Moon, Troy F. Langford, Kassi Taylor Stein, Richard W. Tothill, Yingbin Ouyang, Arthur S. Tischler
ENDOCRINE-RELATED CANCER
(2020)
Article
Endocrinology & Metabolism
Sudhir Kumar Rai, Fernando Bril, Heather M. Hatch, Yiling Xu, Laura Shelton, Srilaxmi Kalavalapalli, Arielle Click, Douglas Lee, Chris Beecher, Austin Kirby, Kimi Kong, Jose Trevino, Abhishek Jha, Shashank Jatav, Kriti Kriti, Soumya Luthra, Timothy J. Garrett, Joy Guingab-Cagmat, Daniel Plant, Prodip Bose, Kenneth Cusi, Robert A. Hromas, Arthur S. Tischler, James F. Powers, Priyanka Gupta, James Bibb, Felix Beuschlein, Mercedes Robledo, Bruna Calsina, Henri Timmers, David Taieb, Matthias Kroiss, Susan Richter, Katharina Langton, Graeme Eisenhofer, Raymond Bergeron, Karel Pacak, Sergei G. Tevosian, Hans K. Ghayee
METABOLISM-CLINICAL AND EXPERIMENTAL
(2020)
Article
Oncology
Lauren Fishbein, Ignaty Leshchiner, Vonn Walter, Ludmila Danilova, A. Gordon Robertson, Amy R. Johnson, Tara M. Lichtenberg, Bradley A. Murray, Hans K. Ghayee, Tobias Else, Shiyun Ling, Stuart R. Jefferys, Aguirre A. de Cubas, Brandon Wenz, Esther Korpershoek, Antonio L. Amelio, Liza Makowski, W. Kimryn Rathmell, Anne-Paule Gimenez-Roqueplo, Thomas J. Giordano, Sylvia L. Asa, Arthur S. Tischler, Karel Pacak, Katherine L. Nathanson, Matthew D. Wilkerson