Article
Chemistry, Analytical
Adam J. Anthony, Amit K. S. Gautam, Lohra M. Miller, Yiran Ma, Anya G. Hardwick, Anu Sharma, Subhadip Ghatak, Andreas Matouschek, Martin F. Jarrold, David E. Clemmer
Summary: Charge detection mass spectrometry (CDMS) was used to study proteasomes and their oligomers. Mass measurements of 20S, 19S, 26S, and 30S proteasomes from Saccharomyces cerevisiae were conducted, and larger assemblies (20S)(x) and (19S)(x) were observed under certain conditions. The findings contribute to a better understanding of proteasomes and their oligomeric states.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Adam J. Anthony, Amit K. S. Gautam, Lohra M. Miller, Yiran Ma, Anya G. Hardwick, Anu Sharma, Subhadip Ghatak, Andreas Matouschek, Martin F. Jarrold, David E. Clemmer
Summary: This study examined the use of Charge Detection Mass Spectrometry (CDMS) to study proteasomes. The masses of different proteasomes were measured and larger assemblies were observed under certain conditions. The study also discussed possible structures for the formation of these assemblies and the utility of CDMS in characterizing proteasomes and related oligomers.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Brett Vahkal, Jamie Kraft, Emanuela Ferretti, Minyoung Chung, Jean-Francois Beaulieu, Illimar Altosaar
Summary: Proteomics can map extracellular vesicles (EVs), such as exosomes, across disease states and cell types, but careful consideration must be given to avoid the impact of contaminants in samples. Human milk EVs and exosomes are increasingly important in health and disease research, yet experimental characterization and functional assays still need further refinement.
Article
Chemistry, Analytical
Shuwen Hua, Bing Wang, Chuan-Fan Ding, Yinghua Yan
Summary: The analysis of low abundance phosphopeptides and specific capture exosomes is crucial in understanding the pathogenesis of diseases. A carbon-based material, G@C@Ti-Zr-HMCT, was developed using titanium-zirconium ions and dopamine and polyimide tubes. This material showed satisfactory capability of enriching phosphopeptides and capturing exosomes.
Review
Multidisciplinary Sciences
Lloyd M. Smith, Jeffrey N. Agar, Julia Chamot-Rooke, Paul O. Danis, Ying Ge, Joseph A. Loo, Ljiljana Pasa-Tolic, Yury O. Tsybin, Neil L. Kelleher
Summary: Understanding the structure and properties of proteins is crucial in deciphering biological functions. The concept of defining the human proteome as a reference set of proteoforms is proposed, with a call for improved technologies in a two-pronged strategy.
Review
Chemistry, Analytical
Deying Chen, Shuang Zhao, Lanjuan Li, Liang Li
Summary: Metabolome analysis is crucial in clinical and biological applications, but controversy exists due to different analytical methods used. This review discusses the potential impacts of each pre-analytical step on metabolome analysis and warns against generalizing study findings.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Henry Oppermann, Stefanie Elsel, Claudia Birkemeyer, Juergen Meixensberger, Frank Gaunitz
Summary: The study demonstrates that erythrocytes can uptake and protect carnosine from degradation, with no negative impact on ATP production and defense against oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Jiakai Chen, Bing Wang, Yiting Luo, Weimin Wang, Chuan-Fan Ding, Yinghua Yan
Summary: In this study, a facile and low-cost strategy was used to synthesize zirconium-rich porphyrin-based porous organic polymers (Imi-Pops-Zr) for specific enrichment and isolation of phosphor-ylated peptides and exosomes. The material demonstrated high selectivity, low detection limit, and a loading capability for phosphopeptides. The experimental results showed promising applications of Imi-Pops-Zr in enriching and isolating phosphorylated peptides and exosomes in complex bio-samples.
Article
Chemistry, Analytical
Bo Sun, Jiuyang Liu, Pei Cai, Jianhua Wu, Wei Liu, Hankun Hu, Liang Liu
Summary: In this study, a simple and accurate LC-MS/MS assay was developed to quantify trastuzumab in human serum using aptamers for sample purification. The method involved extraction of trastuzumab from serum samples, followed by reduction, alkylation, trypsin digestion, and quantification using LC-MS/MS. The assay provided a novel approach for accurate and quantitative monitoring of mAb-trastuzumab.
Article
Multidisciplinary Sciences
Denise Wolrab, Robert Jirasko, Eva Cifkova, Marcus Horing, Ding Mei, Michaela Chocholouskova, Ondrej Peterka, Jakub Idkowiak, Tereza Hrnciarova, Ladislav Kuchar, Robert Ahrends, Radana Brumarova, David Friedecky, Gabriel Vivo-Truyols, Pavel Skrha, Jan Skrha, Radek Kucera, Bohuslav Melichar, Gerhard Liebisch, Ralph Burkhardt, Markus R. Wenk, Amaury Cazenave-Gassiot, Petr Karasek, Ivo Novotny, Kristina Greplova, Roman Hrstka, Michal Holcapek
Summary: Pancreatic cancer has the worst prognosis among all cancers. Cancer screening of body fluids may improve the survival time prognosis of patients. The study found significant differences in blood lipids between pancreatic cancer patients and healthy controls.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Min He, Wenxiang Zhang, Zheyi Liu, Lingqiang Zhou, Xiaoming Cai, Ruibin Li, Yuanjiang Pan, Fangjun Wang
Summary: In this study, the molecular interaction details of human serum albumin (HSA) with Au and SiO2 nanoparticles were systematically investigated using lysine reactivity profiling mass spectrometry (LRP-MS). The results demonstrated that Au nanoparticles exhibited better biocompatibility than SiO2 nanoparticles, and the surface charge state of the nanoparticles played a more important role in the combination with HSA than particle size in the range of 15-40 nm.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Elise K. Mullins, Thomas W. Powers, Jim Zobel, Kory M. Clawson, Lauren F. Barnes, Benjamin E. Draper, Qin Zou, Joseph J. Binder, Stanley Dai, Kun Zhang, Olga Friese, Herbert A. Runnels, Martin F. Jarrold, Lawrence C. Thompson
Summary: This study observed differential infectivity and product yield between two recombinant chimpanzee adenovirus C68 constructs with different genome lengths. Analysis of the empty and packaged capsids showed multiple species with varying abundance between the virus builds. The study also confirmed the identity of packaging variants using charge detection mass spectrometry, and found that liquid chromatography with fluorescence detection is a superior methodology for viral particle titer.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mikel Azkargorta, Ibon Iloro, Iraide Escobes, Diana Cabrera, Juan M. Falcon-Perez, Felix Elortza, Felix Royo
Summary: The study enriched extracellular vesicles fraction from human serum using different techniques and found that ultracentrifugation yielded a unique protein set, while size exclusion chromatography removed lipoprotein-associated proteins. A polymer-based precipitation kit showed preference for proteins associated with platelet granules, while another kit targeting glycosylation molecules enriched proteins with glycosylation sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Peter G. Miller, Murugappan Sathappa, Jamie A. Moroco, Wei Jiang, Yue Qian, Sumaiya Iqbal, Qi Guo, Andrew O. Giacomelli, Subrata Shaw, Camille Vernier, Besnik Bajrami, Xiaoping Yang, Cerise Raffier, Adam S. Sperling, Christopher J. Gibson, Josephine Kahn, Cyrus Jin, Matthew Ranaghan, Alisha Caliman, Merissa Brousseau, Eric S. Fischer, Robert Lintner, Federica Piccioni, Arthur J. Campbell, David E. Root, Colin W. Garvie, Benjamin L. Ebert
Summary: This study elucidates the molecular mechanism of GSK2830371 activity by using computational, biochemical and functional genetic studies. The data shows that GSK2830371 binds to an allosteric site of PPM1D with high affinity and shifts the equilibrium to the inactive form. Additionally, it is found that C-terminal truncating mutations destabilize the protein. These findings provide insights into the biology of PPM1D.
NATURE COMMUNICATIONS
(2022)
Review
Biotechnology & Applied Microbiology
Sun Jiawei, Chen Zhi, Tian Kewei, Li Xiaoping
Summary: In this study, commonly used exosome isolation approaches are reviewed, with a focus on magnetic bead-based capture, which offers high purity and integrity of exosomes. The challenges of traditional methods for exosome isolation are also described, highlighting the directions for future research and development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Yeoseon Kim, Dabin Lee, Jeongkwon Kim
ANALYTICAL BIOCHEMISTRY
(2019)
Article
Biochemical Research Methods
Hyemin Choi, Dabin Lee, Yeoseon Kim, Huu-Quang Nguyen, Sol Han, Jeongkwon Kim
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2019)
Article
Polymer Science
Min Sup Park, Joonho Kim, Jeongkwon Kim, Jinsook Hong, Young-Wun Kim, Jae Hee Song, Yeong-Joon Kim
Article
Chemistry, Applied
Joung Boon Hwang, Subi Lee, Jinhwa Yeum, MeeKyung Kim, Jae Chun Choi, Se-Jong Park, Jeongkwon Kim
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2019)
Letter
Biochemical Research Methods
Huu-Quang Nguyen, Dabin Lee, Yeoseon Kim, Min Sun Kim, Kyoung-Soon Jang, Jeongkwon Kim
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2019)
Article
Physics, Atomic, Molecular & Chemical
Somayeh Maleki, Dabin Lee, Yeoseon Kim, Jeongkwon Kim
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2019)
Article
Chemistry, Analytical
Huu-Quang Nguyen, Dabin Lee, Yeoseon Kim, Minseok Paek, Minsun Kim, Kyoung-Soon Jang, Jooyeon Oh, Young-Sun Lee, Jong Eun Yeon, David M. Lubman, Jeongkwon Kim
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Applied
Hyunjeong Ju, Byungjoo Kim, Jeongkwon Kim, Song-Yee Baek
Article
Biochemical Research Methods
Jihyeon Lee, Min Sun Kim, Hyun Sik Kim, Yoong-Kee Choe, Soo Gyeong Cho, Eun Mee Goh, Jeongkwon Kim
Summary: This study comprehensively characterized the adduct ions of RDX and HMX using ultrahigh resolution and accuracy 15-T FT-ICR MS, and discovered new adduct ions. Different types of adduct ions were observed using different ionization sources. The identification of the [M + NO2](-) adduct ion in the ESI-MS analysis of RDX and HMX is a novel finding reported for the first time.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Chemistry, Applied
Dabin Lee, Yeoseon Kim, Iqbal Jalaludin, Huu-Quang Nguyen, Minsun Kim, Jungju Seo, Kyoung-Soon Jang, Jeongkwon Kim
Summary: Disaccharides are sugars composed of two monosaccharides linked by a glycosidic linkage, with specific properties determined by the type of linkage and the identity of the monosaccharides. Using MALDI-MS with a graphene oxide matrix, seven disaccharide isomers were analyzed and their unique cleavage patterns were identified. The study demonstrated a strong linear correlation between the relative peak intensity of a sucrose fragment peak and the amount of sucrose in a mixture, which was successfully applied to quantify maltose and sucrose in different honey samples.
Article
Chemistry, Physical
Taejun Kim, Ju Hyun Yang, So Jeong Park, Huu-Quang Nguyen, Jeongkwon Kim, Ki-Ju Yee, Heesoo Jung, Jun-Gill Kang, Youngku Sohn
Summary: In this study, a dry-photodecontamination method was employed to treat CWNA model molecules contaminated on aluminum and oxidized aluminum, deepening the understanding of the photo-decomposition mechanism of CWNAs. The research also investigated the characterization of solid residues and electronic energy state calculation, providing new insights for the development and application of dry UV light photo-decontamination methods.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2021)
Article
Chemistry, Analytical
Younsu Kim, Jihyung Seo, Yoong Kee Choe, Youngku Sohn, Jeongkwon Kim
Summary: The flame retardant mixtures of DBDPE and Sb2O3 were analyzed using FT-IR spectroscopy, with experimental and theoretical wavenumbers compared. A strong correlation between mixing molar ratios and observed peak area ratios was observed, indicating the potential of FT-IR analysis for determining relative amounts of components in flame retardant mixtures.
ANALYTICAL SCIENCE AND TECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Sintayehu Kebede Gurmessa, Lemma Teshome Tufa, Jeonghyo Kim, Kang-In Lee, Young-Mi Kim, Van Tan Tran, Huu-Quang Nguyen, Tae Sun Shim, Jeongkwon Kim, Tae Jung Park, Jaebeom Lee, Hwa-Jung Kim
Summary: The study developed a colorimetric ELISA technique using nanozyme probes to detect CFP-10 antigens secreted by MTB for early tuberculosis diagnosis. The method showed high accuracy in discriminating active TB from other samples and has potential clinical applications.
ACS APPLIED NANO MATERIALS
(2021)
Article
Spectroscopy
Jangmi Hong, Jeongkwon Kim
MASS SPECTROMETRY LETTERS
(2020)
Article
Spectroscopy
Dahee Kim, Minhee Joo, Dabin Lee, Huu-Quang Nguyen, Jeongkwon Kim
MASS SPECTROMETRY LETTERS
(2019)