Journal
ELECTROPHORESIS
Volume 37, Issue 7-8, Pages 1101-1108Publisher
WILEY
DOI: 10.1002/elps.201500196
Keywords
Cholesterol oxidation products; Cypriot food products; Tandemmass spectrometry; Ultra-performance liquid chromatography
Funding
- Republic of Cyprus
- European Regional Development Fund through the Research Promotion Foundation of Cyprus
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This paper reports the development and validation of a new method based on ultraperformance LC coupled to MS/MS for the simultaneous determination of four cholesterol oxidation products (COPs) in foodstuffs in only 4.1 min. The COPs were detected by ESI in positive-ion mode with multiple reaction monitoring, and the mass spectrometric conditions were optimized in order to increase sensitivity. The developed method was validated in terms of linearity, precision, LODs, and LOQs. Recoveries of the extraction process ranged from 86 to 98.5% when the samples were fortified at 100, 500, and 1500 ng/mL. The applicability of the method was confirmed by analyzing different food samples. Considering the paucity of data regarding the content of COPs in Cypriot foods, particular attention was devoted, for the first time, to the determination of the profile of the main COPs in widely consumed, traditional Cypriot foodstuffs (halloumi cheese, hiromeri, snails, etc.)
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