Article
Biochemical Research Methods
Qinhong Wang, Wenqiang Han, Chuanzhen Ma, Tianyu Wang, Jingquan Zhong
Summary: Recent research questions the validity of housekeeping proteins in Western blot. This study proposed new ideas for normalization in Western blot, improving reproducibility. Using the GEO database and GEO2R tool, unstable housekeeping genes were quickly excluded. The reliability of GAPDH was examined by Western blot, and unstable housekeeping genes were found in other cardiovascular animal models. A modified Ponceau S staining method was also proposed, providing researchers with a proper loading control for Western blot and improving reproducibility in research.
Article
Biochemical Research Methods
Sudeep Musyaju, Hiren R. Modi, William J. Flerlage, Anke H. Scultetus, Deborah A. Shear, Jignesh D. Pandya
Summary: Mitochondrial dysfunction plays a dominant role in TBI pathophysiology. This study found changes in mitochondrial HKP expression following PTBI. The RTP method for protein normalization showed higher sensitivity and effectiveness compared to the traditional HKPs method.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Biochemical Research Methods
Jonathan S. Thacker, Delaney Andersen, Stanley Liang, Natalia Zieniewicz, Juan S. Trivino-Paredes, Patrick C. Nahirney, Brian R. Christie
Summary: A series of antigen-retrieval steps have been developed to make fixed brain lysates suitable for Western blot analysis, successfully revealing a variety of proteins. This protocol has significant utility for future studies using fixed tissue samples in different neuropathological conditions.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Biochemical Research Methods
Peter-Philip M. Booth, Don T. Lamb, Jon P. Anderson, Michael D. Furtaw, Robert T. Kennedy
Summary: In this study, a novel Western blotting system using capillary gel electrophoresis was developed for protein separation and quantification. This system offers advantages of easy operation and reduced sample requirements, and was successfully applied in detecting multiple proteins.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Aristeidis Ntoukas, Athanasios Niarchos, Aikaterini C. Tsika, Spiridon Mantzoukas, Georgios A. Spyroulias, Konstantinos Poulas
Summary: This study presents a simple and reliable assay for protein detection and quantification in molecular biological experiments, showing higher reliability and linearity compared to the traditional Western blot technique.
Article
Agriculture, Multidisciplinary
Yin Ling, Zhu Jimin, Liu Caixing, Tian Xuhong, Zhang Sumin
Summary: Automatic body size measurement of livestock using 3D vision technology is a current research focus, where the accuracy is affected by the postures. A standard posture classification algorithm was proposed, along with the adjustment of nonstandard postures based on joint point vectors eigenvalues to improve the accuracy of three-dimensional measurement.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2022)
Article
Biotechnology & Applied Microbiology
Jun-Ling Wang, Mei-Qi Li, Jia-Jia Zhang, Chao-Jin Xu
Summary: The conventional internal loading control proteins are not suitable for serum, extracellular secretion, cerebrospinal fluid analysis, or protein purification due to variation in different cell states and tissue types. Congo red staining for total protein measurement is proposed as a superior alternative with advantages of lower cost, technical simplicity, and improved linear regression for evaluating protein loading in western blots.
BIOTECHNIC & HISTOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Christopher T. Desire, R. Dario Arrua, Xanthe L. Strudwick, Zlatko Kopecki, Allison J. Cowin, Emily F. Hilder
Summary: Significant technical advancements have been made in the field of western blotting through the use of microfluidic technologies over the past two decades. This review provides a critical overview of these advancements, discussing the advantages and disadvantages of each approach. The development of commercially available systems, including those for single cell analysis, is highlighted. The review also covers recent developments such as automation algorithms, different materials/chemistries, projection electrophoresis, and triBlots, as well as the potential of these systems for point-of-care devices in healthcare.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Bing Liu, Hanyu Tang, Qian Liu, Wenwen Wang, Haitao Li, Shiya Zheng, Fei Sun, Xiangwei Zhao
Summary: In this study, a novel surface-enhanced Raman scattering-based Western blot (SERS-WB) was proposed for accurate quantitative detection of low-abundance proteins. SERS nanotags were used as quantitative labels of proteins, and the target protein on the membrane could be directly identified by colorimetric signal due to the aggregation effect of nanoparticles.
Article
Microbiology
Maria de la Luz Galvan-Ramirez, Claudia Charles-Nino, Cesar Pedroza-Roldan, Carolina Salazar-Reveles, Karen Lissete Ocampo-Figueroa, Laura Rocio Rodriguez-Perez, Varinia Margarita Paez-Magallan
Summary: This study investigated the prevalence of Toxoplasma gondii infection in cats from the metropolitan region of Guadalajara in western Mexico. The results showed a low prevalence of T. gondii infection in cats in this area, with age being a major risk factor.
Article
Engineering, Civil
Alessandro Pisapia, Elide Nastri, Vincenzo Piluso, Antonio Formisano, Federico Massimo Mazzolani
Summary: In this paper, an extensive experimental campaign is conducted on 5000, 6000 and 7000 aluminium series to characterize their behavior in terms of stress-strain curve, low cycle fatigue, cyclic hardening, dissipated energy and plastic fracture. The knowledge gained from this research can be beneficial for understanding the nonlinear behavior of these alloys, particularly under seismic loading. The collected information is also important for the revision of the new Eurocode 8, which includes a section on seismic design of structures made of aluminium alloys.
ENGINEERING STRUCTURES
(2023)
Article
Biochemical Research Methods
Zhen Wang, Ziyang Li, Tian Su, Xiao Han, Zhanwu Hou, Yupeng Zheng, Jiachen Liu, Jun Xu, Jeffy Yang, Huadong Liu
Summary: Western blot is a widely used analytical technique in molecular biology. A new strategy using a probe with proximal labeling and IgG targeting abilities can amplify the signal, enhancing the sensitivity in protein identification. This strategy may be combined with other signal enhancement methods to further increase the sensitivity of Western blot.
Article
Biochemical Research Methods
You-Rim Cha, Saba Sajjad, Kyung Ho Han, Tae-Sung Yoon, Heung-Seon Shin, Sunghyun Kang
Summary: S-CDR and R-CDR devices, utilizing spreader-rod technology, offer significantly enhanced results compared to traditional batch incubation methods in western blotting. The devices not only improve signal strength and speed of development, but also provide benefits such as uniform antibody binding, reduced antibody usage, and the ability to recover results from mishandled membranes. The S-CDR device is easily adaptable to existing western blot protocols and ensures higher quality blots.
Article
Agricultural Engineering
Young-bae Choi, Jun-gyu Kim, Jeong-hwa Cho, Sang-yeon Lee, Se -jun Park, Cristina Decano-Valentin, Deuk-young Jeong, In-bok Lee
Summary: In mechanically ventilated piglet houses, the most important aspect to improve the rearing environment is ventilation. Pressure losses can cause the airflow rate of fans to be lower than the design rate. This study evaluated pressure losses and developed a ventilation rate formula based on field experiments, which can be applied for the design of ventilation systems in piglet houses.
BIOSYSTEMS ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Mona Reichel, Verena Weitzel, Laura Klement, Carsten Hoffmann, Julia Drube
Summary: G protein-coupled receptor kinases (GRKs) regulate G protein-coupled receptors (GPCRs) by phosphorylating the active receptors. In addition to their role in GPCRs, GRKs also regulate a variety of proteins by acting as scaffolding partners. By examining the specificity of commercially available antibodies, we established a cost-effective method for comparing protein levels obtained by Western blotting with different antibodies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Julie A. Wixey, Kirat K. Chand, Lily Pham, Paul B. Colditz, S. Tracey Bjorkman
JOURNAL OF PHYSIOLOGY-LONDON
(2018)
Article
Pharmacology & Pharmacy
Barbara E. Lingwood, Yvonne A. Eiby, Stella T. Bjorkman, Stephanie M. Miller, Ian M. R. Wright
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2019)
Article
Immunology
Julie A. Wixey, Kah Meng Lee, Stephanie M. Miller, Kate Goasdoue, Paul B. Colditz, S. Tracey Bjorkman, Kirat K. Chand
JOURNAL OF NEUROINFLAMMATION
(2019)
Article
Developmental Biology
Kate Goasdoue, Kirat Kishore Chand, Stephanie Melita Miller, Kah Meng Lee, Paul Bernard Colditz, Julie Anne Wixey, Stella Tracey Bjorkman
DEVELOPMENTAL NEUROSCIENCE
(2019)
Article
Physiology
Julie A. Wixey, Kishen R. Sukumar, Rinaldi Pretorius, Kah Meng Lee, Paul B. Colditz, S. Tracey Bjorkman, Kirat K. Chand
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Neurosciences
Sophie Casey, Kate Goasdoue, Stephanie M. Miller, Gary P. Brennan, Gary Cowin, Adam G. O'Mahony, Christopher Burke, Boubou Hallberg, Geraldine B. Boylan, Aideen M. Sullivan, David C. Henshall, Gerard W. O'Keeffe, Catherine Mooney, Tracey Bjorkman, Deirdre M. Murray
MOLECULAR NEUROBIOLOGY
(2020)
Article
Neuroimaging
Paul Nichols, Javier Urriola, Stephanie Miller, Tracey Bjorkman, Kate Mahady, Viktor Vegh, Fatima Nasrallah, Craig Winter
Summary: This study revealed a correlation between DCE measures and MMP values in patients with TBI, suggesting that serum MMP-7 may be considered as a peripheral biomarker for quantifying BBB dysfunction in TBI patients.
NEUROIMAGE-CLINICAL
(2021)
Article
Anatomy & Morphology
Stephanie M. Miller, Samuel Pelly, Viskasari P. Kalanjati, Aven Lee, Paul B. Colditz, S. Tracey Bjorkman
BRAIN STRUCTURE & FUNCTION
(2018)