Article
Fisheries
Jemma V. Milburn, Anna Hoog, Sonia Villanueva-Hernandez, Kerstin H. Mair, Wilhelm Gerner
Summary: Progress has been made in the study of regulatory B cells (IL-10 producing) in pigs, with the identification of a small subset of IL-10 competent cells within both porcine B1 and B2 cell subsets. The differentiation status of these cells varies across different tissues. These findings support the emerging paradigm that B cell IL-10 production is regulated by activation history and microenvironmental factors.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jin Kyeong Choi, Cheng-Rong Yu, So Jin Bing, Yingyos Jittayasothorn, Mary J. Mattapallil, Minkyung Kang, Seung Bum Park, Hyun-Su Lee, Lijin Dong, Guangpu Shi, Rachel R. Caspi, Charles E. Egwuagu
Summary: Natural regulatory B-1a cells producing IL-27 (i27-Bregs) have been identified in the peritoneal cavity and human umbilical cord blood, accumulating in the CNS during neuroinflammation to confer protection against autoimmune diseases. i27-Breg immunotherapy ameliorated encephalomyelitis and uveitis through up-regulating inhibitory receptors, suppressing immune responses, and converting conventional B cells to secrete IL-10 and/or IL-35.Mutation of irf4 in B cells resulted in increased i27-Bregs with few i35-Bregs, while loss of irf8 led to an abundance of i35-Bregs but impaired generation of i27-Bregs, suggesting the role of IRF8/BATF and IRF4/BATF axis in regulating B cell differentiation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Yongsheng Luo, Feifei Luo, Kuanxin Zhang, Shilei Wang, Haojie Zhang, Xianlei Yang, Wenjun Shang, Junxiang Wang, Zhigang Wang, Xinlu Pang, Yonghua Feng, Lei Liu, Hongchang Xie, Guiwen Feng, Jinfeng Li
Summary: Circulating IL-10(+) Breg levels are a more appropriate indicator for assessing resistance to AMR after kidney transplantation compared to Bregs. Kidney B cell and IL-10 infiltration, as well as high expression of CXCL13, were associated with AMR. Furthermore, circulating IL-10(+) Bregs remained elevated during the 90-day post-operation period, contributing to immune homeostasis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Akimichi Inaba, Zewen Kelvin Tuong, Tian X. X. Zhao, Andrew P. P. Stewart, Rebeccah Mathews, Lucy Truman, Rouchelle Sriranjan, Jane Kennet, Kourosh Saeb-Parsy, Linda Wicker, Frank Waldron-Lynch, Joseph Cheriyan, John A. A. Todd, Ziad Mallat, Menna R. R. Clatworthy
Summary: Activation of B cells expressing CD25 can be enhanced by low-dose recombinant IL-2, leading to increased secretion of IL-10. Administration of low dose IL-2 to patients results in an increase in IL-10-producing B cells. Single-cell RNA sequencing reveals enrichment of regulatory B cell gene signature in plasma cell populations from IL-2-treated patients, with significant down-regulation of the transcriptional repressor BACH2. BACH2 suppression leads to increased B cell IL-10, suggesting its role in promoting an immunoregulatory phenotype in B cells through IL-2 modulation.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Meiling Wu, Sulan Yu, Yacun Chen, Wei Meng, Haiyong Chen, Jing He, Jiangang Shen, Xiang Lin
Summary: IL-10-producing regulatory B (Breg) cells are important for immune tolerance. This study found that acteoside (AC), a compound from a medicinal herb, can promote IL-10 production from B cells by regulating the TLR4/PI3K axis. AC treatment increased IL-10(+) B cells and improved disease pathology in a mouse model of primary Sjogren's syndrome (pSS).
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jin Kyeong Choi, Charles E. Egwuagu
Summary: B lymphocytes play a central role in host immunity by orchestrating humoral immune responses and producing neutralizing antibodies. Recent recognition shows that B cells also help maintain peripheral tolerance and suppress autoimmune diseases, with rare populations of regulatory B cells producing anti-inflammatory cytokines. The newly discovered i35-Bregs can suppress autoimmune diseases by inducing infectious tolerance in conventional B cells.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Chelsea L. Edwards, Susanna S. Ng, Fabian de Labastida Rivera, Dillon Corvino, Jessica A. Engel, Marcela Montes de Oca, Luzia Bukali, Teija C. M. Frame, Patrick T. Bunn, Shashi Bhushan Chauhan, Siddharth Sankar Singh, Yulin Wang, Jinrui Na, Fiona H. Amante, Jessica R. Loughland, Megan S. F. Soon, Nicola Waddell, Pamela Mukhopadhay, Lambros T. Koufariotis, Rebecca L. Johnston, Jason S. Lee, Rachel Kuns, Ping Zhang, Michelle J. Boyle, Geoffrey R. Hill, James S. McCarthy, Rajiv Kumar, Christian R. Engwerda
Summary: Control of malaria requires Th1 cells with IL-10 to mitigate inflammation, but IL-10+Tr1 cells can also promote parasite persistence or impair immunity. Molecular and phenotypic signatures distinguish IL-10-Th1 cells from IL-10+Tr1 cells in malaria-infected individuals and mice, and a conserved Tr1 cell signature is found in patients with malaria, dengue, and graft-versus-host disease.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Multidisciplinary Sciences
Anett Mazlo, Ramona Kovacs, Noemi Miltner, Marta Toth, Zoltan Vereb, Krisztina Szabo, Ildiko Bacskai, Kitti Pazmandi, Agota Apati, Tamas Biro, Krisztian Bene, Eva Rajnavolgyi, Attila Bacsi
Summary: Findings suggest that MSCl cells promote differentiation of CTLA-4 expressing DCs through the production of all-trans retinoic acid, leading to the activation and polarization of allogeneic, naive T cells by semi-matured DCs in a CTLA-4 dependent manner. Understanding the molecular mechanisms behind MSC-mediated modulation of DC differentiation may expand the clinical application of MSCs in cell-free therapies.
Article
Oncology
Krzysztof Piersiala, Eric Hjalmarsson, Pedro Farrajota Neves da Silva, Vilma Lagebro, Aeneas Kolev, Magnus Starkhammar, Alexandra Elliot, Linda Marklund, Eva Munck-Wikland, Gregori Margolin, Susanna Kumlien Georen, Lars-Olaf Cardell
Summary: It has been established that T cells play a significant role in anti-tumor immune response, while the contribution of B cells has been insufficiently studied. In this research, the proportions and characteristics of B cells in tumor draining lymph nodes (TDLNs) and non-TDLNs from oral squamous cell carcinoma patients were analyzed. The results showed that TDLNs had a higher proportion of B cells, especially immunosuppressive regulatory B cells, compared to non-TDLNs. The presence of regulatory B cells in TDLNs may hinder the effectiveness of novel cancer immunotherapies in head and neck cancer.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Review
Immunology
Hui Sun, Yuzhang Wu, Yi Zhang, Bing Ni
Summary: This study focuses on the production mechanisms of IL-10 in ILCs, the key role of IL-10 in NK and ILC2 cells, and the potential impact of IL-10-producing ILCs in diseases such as infections, allergies, and cancers.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Yikai Liu, Zhiying Chen, Junlin Qiu, Hongzhi Chen, Zhiguang Zhou
Summary: Our study revealed altered Tim-1 and IL-10 expression in regulatory B cells in T1D patients. Tim-1, as shown by the study, is associated with islet function and blood glucose levels, indicating that Tim-1(+) Bregs and IL-10(+) Bregs are involved in the pathogenesis of T1D.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Dongwen Wu, Catherine H. Poholek, Saikat Majumder, Qixing Liu, Shankar K. Revu, Kanishka Mohib, David M. Rothstein, Mandy J. McGeachy
Summary: Prior exposure to pathogen-associated stimuli can reduce morbidity and mortality to new infections through innate immune training, which can be further improved by childhood vaccination. This study demonstrates that inflammatory training of fibroblastic reticular cells (FRC) in the gut enhances immune responses to bacterial pathogens and reduces colon inflammation through IL-10 production by B cells, ultimately improving clearance of infection. These findings suggest a new mode of immune training through FRC that can impact future adaptive responses and better preserve host health.
SCIENCE IMMUNOLOGY
(2021)
Article
Biology
Meenu Kalkal, Rubika Chauhan, Reva Sharan Thakur, Mrinalini Tiwari, Veena Pande, Jyoti Das
Summary: Regulatory B cells play a crucial role in immune protection during infection with malaria parasites by controlling inflammation and inhibiting parasite growth. Adoptive transfer of these cells can slow down parasite growth and enhance survival in infected animals.
Article
Multidisciplinary Sciences
Lihi Radomir, Matthias P. Kramer, Michal Perpinial, Nofar Schottlender, Stav Rabani, Keren David, Anna Wiener, Hadas Lewinsky, Shirly Becker-Herman, Rina Aharoni, Ron Milo, Claudia Mauri, Idit Shachar
Summary: Regulatory B (Breg) cells suppress excessive inflammation primary via the production of interleukin 10 (IL-10). Here the authors show that the function and homeostasis of mouse and human IL-10(+) Breg cells are negatively regulated by the cell surface receptor, SLAMF5, to impact experimental autoimmunity, thereby hinting SLAMF5 as a potential target for immunotherapy.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Jinqiu Jiang, Tao Qin, Liang Zhang, Qiao Liu, Jiabin Wu, Rongxin Dai, Lina Zhou, Qin Zhao, Xiaoyan Luo, Hua Wang, Xiaodong Zhao
Summary: Deficiencies in DOCK8 result in quantitative and qualitative defects in IL-10-producing Breg cells, potentially due to abnormal Dock8(-/-) CD4(+) T cells. The study found that recombinant murine IL-21 can restore Bregs function and reduce inflammatory cell infiltration in the lung in a murine asthma model. This provides new insights into potential therapeutic strategies for allergic diseases, such as asthma associated with DOCK8 deficiency.
FRONTIERS IN IMMUNOLOGY
(2021)
