Article
Rheumatology
David Simon, Koray Tascilar, Katja Schmidt, Bernhard Manger, Leonie Weckwerth, Maria Sokolova, Laura Bucci, Filippo Fagni, Karin Manger, Florian Schuch, Monika Ronneberger, Axel Hueber, Ulrike Steffen, Dirk Mielenz, Martin Herrmann, Thomas Harrer, Arnd Kleyer, Gerhard Kroenke, Georg Schett
Summary: The study found that B cell depletion completely blocks humoral response to SARS-CoV-2 vaccination, but not T cell response. Limited humoral immune responses were observed in patients with B cell depletion after SARS-CoV-2 infection.
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Gastroenterology & Hepatology
Moritz Leppkes, Aylin Lindemann, Stefanie Goesswein, Susanne Paulus, Dominik Roth, Anne Hartung, Eva Liebing, Sebastian Zundler, Miguel Gonzalez-Acera, Jay Patankar, Fabrizio Mascia, Kristina Scheibe, Markus Hoffmann, Stefan Uderhardt, Christine Schauer, Sebastian Foersch, Clemens Neufert, Michael Vieth, Georg Schett, Raja Atreya, Anja A. Kuehl, Andre Bleich, Christoph Becker, Martin Herrmann, Markus F. Neurath
Summary: Bleeding ulcers and erosions are common in active ulcerative colitis, with fibrin layers correlated with rectal bleeding. Neutrophils induce secondary immunothrombosis through PAD4-dependent mechanisms, protecting against acute colitis and rectal bleeding. Deficiency in immunothrombosis can lead to exacerbated colitis and increased bleeding.
Article
Dermatology
Kata P. Szilveszter, Simon Vikar, Adam Horvath, Zsuzsanna Helyes, Miklos Sardy, Attila Mocsai
Summary: Phospholipase C gamma 2 (PLC gamma 2) plays a critical role in the pathogenesis of inflammatory epidermolysis bullosa acquisita by regulating the tissue infiltration of immune cells and the release of proinflammatory mediators.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Dermatology
Tamas Nemeth, Lili Balogh, Eszter Kaposztas, Kata P. Szilveszter, Attila Mocsai
Summary: Autoantibodies against C7 trigger skin disease in epidermolysis bullosa acquisita. Syk tyrosine kinase plays a crucial role in the disease process, especially in neutrophils. Inhibitors of Syk effectively block the immune response and reduce the dermal-epidermal separation caused by anti-C7 antibodies. Syk inhibition may be a potential therapeutic strategy for epidermolysis bullosa acquisita.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Editorial Material
Cell Biology
Jasmin Knopf, Aparna Mahajan, Luis E. Munoz, Martin Herrmann
Article
Cell Biology
Julia Elrod, Antonia Kiwit, Moritz Lenz, Holger Rohde, Daniela Boernigen, Malik Alawi, Christoph Mohr, Laia Pagerols Raluy, Magdalena Trochimiuk, Jasmin Knopf, Konrad Reinshagen, Martin Herrmann, Michael Boettcher
Summary: This study evaluated the septic volvulus ischemia-reperfusion model and compared it to two established models in mice sepsis. The results showed that midgut volvulus for 15 minutes caused a comparable mortality rate as the other two models, and at 48 hours, there was a decrease in circulating free DNA levels and an increase in bacterial translocation to the spleen/liver and lungs. In addition, DNases were increased in this model compared to the established models. Proteomic analysis revealed an upregulation of several markers of inflammation and tissue damage at 48 hours. Overall, this study demonstrates that midgut volvulus is a stable and physiological model for studying sepsis.
Review
Biochemistry & Molecular Biology
Jeeshan Singh, Michael Boettcher, Maximilian Doelling, Annika Heuer, Bettina Hohberger, Moritz Leppkes, Elisabeth Naschberger, Mirco Schapher, Christine Schauer, Janina Schoen, Michael Stuerzl, Ljubomir Vitkov, Han Wang, Leticija Zlatar, Georg A. Schett, David S. Pisetsky, Ming-Lin Liu, Martin Herrmann, Jasmin Knopf
Summary: Extracellular chromatin, particularly in the form of NETs, plays a crucial role in the progression of various diseases. It functions as a driver of the interferon system, autoantigen, and scaffold for innate immune system proteins. Insufficient clearance of extruded chromatin can contribute to immune-inflammatory and occlusive disorders. This article discusses the cellular events involved in extracellular chromatin release and NET formation, the negative consequences of dysregulated NET formation, and the imbalance between NET formation and clearance. It also explores the role of NET formation in various diseases and injuries, highlighting the importance of targeting chromatin decondensation pathways and promoting extracellular chromatin clearance for effective therapies.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Han Wang, Antonia Margarethe Stehr, Jeeshan Singh, Leticija Zlatar, Arndt Hartmann, Katja Evert, Elisabeth Naschberger, Saskia von Stillfried, Peter Boor, Luis E. E. Munoz, Jasmin Knopf, Michael Stuerzl, Martin Herrmann
Summary: During inflammatory responses, neutrophils utilize various defense mechanisms, such as phagocytosis, degranulation, recruitment of immune cells, secretion of anti-microbials, and release of neutrophil extracellular traps (NETs). Detection of NETs in tissue sections can be challenging due to the high fluorescence signals emitted from condensed nuclear DNA. However, using anti-DNA-IgM antibodies can effectively detect the extended DNA patches of NETs, providing a new perspective for studying neutrophil-associated immune reactions in diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Ljubomir Vitkov, Jeeshan Singh, Christine Schauer, Bernd Minnich, Jelena Krunic, Hannah Oberthaler, Sonja Gamsjaeger, Martin Herrmann, Jasmin Knopf, Matthias Hannig
Summary: The break of the epithelial barrier of gingiva has been a neglected topic despite its significant role in periodontal pathology and transitory bacteraemia. Mechanically induced bacterial translocation in gingiva has been disregarded despite the knowledge of mechanical force effects on tight junctions in other epithelial tissues. In this review, the bacterial, immune, and mechanical factors responsible for the increased permeability and break of the epithelial barrier of inflamed gingiva are discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Krisztina Futosi, Tamas Nemeth, Adam I. Horvath, Clare L. Abram, Simon Tusnady, Clifford A. Lowell, Zsuzsanna Helyes, Attila Mocsai
Summary: Genetic deficiency of myeloid Src-family kinases protects mice from autoinflammatory diseases, including gout and systemic inflammation. Src-family kinases play a critical role in the proinflammatory functions of neutrophils, but not their migratory capacity. Dasatinib, a Src-family inhibitor, inhibits gouty arthritis.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Immunology
Xue Cao, Yanhong Li, Yubin Luo, Tianshu Chu, Hang Yang, Ji Wen, Yi Liu, Yi Zhao, Martin Herrmann
Summary: The formation of neutrophil extracellular traps (NETs) plays a role in infection-related and sterile inflammation. Monosodium urate (MSU) crystals are involved in various diseases as damage-associated molecular patterns (DAMPs). The study demonstrates that the ROS-sensing channel TRPM2 is necessary for MSU crystal-induced NET formation and TRPM2 knockout leads to reduced inflammation. This indicates that TRPM2 could be a potential target for therapeutic intervention.
JOURNAL OF INFLAMMATION-LONDON
(2023)
Article
Immunology
Julia Elrod, Moritz Lenz, Antonia Kiwit, Lina Armbrust, Lavinia Schoenfeld, Konrad Reinshagen, Laia Pagerols Raluy, Christoph Mohr, Ceren Saygi, Malik Alawi, Holger Rohde, Martin Herrmann, Michael Boettcher
Summary: Through optimizing a murine scald model, this study investigated the immune responses to heat induced injury. The study identified the roles of neutrophil extracellular traps (NETs) and DNases in the therapy of burns. It also evaluated the effects of different exposure times on extracellular matrix.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Leticija Zlatar, Thomas Timm, Gunter Lochnit, Rostyslav Bilyy, Tobias Baeuerle, Marco Munoz-Becerra, Georg Schett, Jasmin Knopf, Jens Heichel, Mohammad Javed Ali, Mirco Schapher, Friedrich Paulsen, Martin Herrmann
Summary: Mucopeptide concretions, also known as dacryoliths, are macroscopic stones that commonly block the lacrimal sac. The mechanisms behind their formation are unclear, but various immune cells, including neutrophils, have been confirmed to be involved. This study investigates the role of neutrophils and neutrophil extracellular traps (NETs) in dacryolithiasis using multiple analysis methods, and proposes a mechanism for the formation and growth of mucopeptide concretions involving neutrophils and NETs.
Meeting Abstract
Oncology
Antonia Margarethe Stehr, Guangxia Wang, Richard Demmler, Marc P. Stemmler, Philipp Tripal, Carol I. Geppert, Susanne Merkel, Martin Herrmann, Michael Stuerzl
ONCOLOGY RESEARCH AND TREATMENT
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Maximilien Euler, Jonas Hahn, Martin Herrmann, Andriy Mokhir, Markus Hoffmann
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
