Article
Biochemistry & Molecular Biology
Boleslaw T. Karwowski
Summary: Every day, a large number of DNA lesions are formed in cells due to various factors, and Base Excision Repair is a common repair mechanism. Research has shown that a specific DNA lesion previously thought to be unrepairable can actually be removed under certain conditions, shedding light on new possibilities for DNA damage repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Michal Szewczuk, Karolina Boguszewska, Julia Kazmierczak-Baranska, Boleslaw T. Karwowski
Summary: Ionizing radiation damages cellular mechanisms by inducing DNA lesions such as cdPus, which can affect the activity of repair enzymes. This study found that cdPus lesions influence the initial stages of DNA repair differently based on their position relative to dU. Understanding and eliminating this type of DNA damage is crucial for preventing carcinogenesis.
Article
Biochemistry & Molecular Biology
Kyung-No Son, Hyun Lee, Dhara Shah, Sushma Kalmodia, Ryan Cree Miller, Marwan Ali, Arun Balasubramaniam, Stephanie M. Cologna, Hyunjoon Kong, Deepak Shukla, Vinay Kumar Aakalu
Summary: The Sigma-2 receptor (S2R) is an important endoplasmic reticular protein, with Histatin-1 (Hst1) being its endogenous ligand. Hst1 plays important roles in epithelial wound healing and cell migration.
Article
Plant Sciences
Xuan Feng, Qingzhi Zhang, David J. Clarke, Hai Deng, David O'Hagan
Summary: 3'-O-ss-Glucosyl-4',5'-didehydro-5'-deoxyadenosine 13 is a natural product of Streptomyces calvus and Streptomyces virens, and can also be generated in vitro by the enzyme NucGT. Experimental results using oxygen-18 and deuterium isotopes confirm that 13 is not a biosynthetic precursor to nucleocidin 1.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Immunology
Niels C. Lory, Mikolaj Nawrocki, Martina Corazza, Joanna Schmid, Valea Schumacher, Tanja Bedke, Stephan Menzel, Friedrich Koch-Nolte, Andreas H. Guse, Samuel Huber, Hans-Willi Mittruecker
Summary: This study investigates the function of TRPM2 in T-cell activation and differentiation using TRPM2-deficient mice. The results suggest that TRPM2 does not play a major role in T-cell activation and differentiation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Masaaki Ishibashi, Masakazu Ishii, Shinichiro Yamamoto, Yasuo Mori, Shunichi Shimizu
Summary: The study demonstrates that 5-FU enhances the activation of TRPM2 channel, and inhibition of H2O2-induced Ca2+ concentration increase by 5-FU in Trpm2 KO mice, alleviating the reduction of blood cells induced by 5-FU.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kon-Young Ji, Su-Man Kim, Su-Min Yee, Min-Jae Kim, Yu-Jin Ban, Eun-Mi Kim, Eun-Hee Lee, Ha-Rim Choi, Hyosuk Yun, Chul Won Lee, Chul-Ho Yun, C. Justin Lee, Hyang Burm Lee, Hyung-Sik Kang
Summary: The study identified CypA as a putative ligand for TREM2, showing specific interaction and regulation of TREM2 signaling transduction.
Article
Multidisciplinary Sciences
Jared S. Elenbaas, Upasana Pudupakkam, Katrina J. Ashworth, Chul Joo Kang, Ved Patel, Katherine Santana, In-Hyuk Jung, Paul C. Lee, Kendall H. Burks, Junedh M. Amrute, Robert P. Mecham, Carmen M. Halabi, Arturo Aliso, Jorge Di Paola, Nathan O. Stitziel
Summary: The study identifies a high affinity interaction between SVEP1 and the orphan receptor PEAR1, which promotes phosphorylation of PEAR1 and activation of AKT/mTOR signaling in vascular cells and platelets. Mice lacking SVEP1 exhibit reduced platelet activation, while exogenous SVEP1 induces PEAR1-dependent activation of platelets. Targeting this receptor-ligand interaction may offer a potential therapeutic strategy for cardiovascular and thrombotic diseases.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Akiko Ogawa, Chisae Nagiri, Wataru Shihoya, Asuka Inoue, Kouki Kawakami, Suzune Hiratsuka, Junken Aoki, Yasuhiro Ito, Takeo Suzuki, Tsutomu Suzuki, Toshihiro Inoue, Osamu Nureki, Hidenobu Tanihara, Kazuhito Tomizawa, Fan-Yan Wei
Summary: The study identified N-6-methyladenosine (m(6)A) as a ligand for the human adenosine A3 receptor with higher affinity than unmodified adenosine. Structural modeling revealed the amino acids required for specific binding of m(6)A to the human A3 receptor and demonstrated its dynamic release in response to cytotoxic stimuli and facilitation of type I allergy in vivo. These findings suggest m(6)A as a signaling molecule capable of activating G protein-coupled receptors (GPCRs) and triggering pathophysiological responses, a previously unreported property of RNA modifications.
Article
Cell Biology
MengWen Huang, Ling Lu, ChangDong Lin, YaJuan Zheng, XingChao Pan, ShiHui Wang, ShiYang Chen, YouHua Zhang, ChunYe Liu, GaoXiang Ge, Yi Arial Zeng, JianFeng Chen
Summary: Dynamic regulation of integrin activation and inactivation is crucial for cell adhesion and migration. LRP12 is identified as a transmembrane inhibitor for a4 integrin activation, maintaining the balance of nascent adhesion turnover and controlling cell migration. Knockdown of LRP12 leads to increased nascent adhesions and enhanced cell migration.
Article
Chemistry, Multidisciplinary
Ryo Miyahara, Yosuke Taniguchi
Summary: The formation of unnatural base pairs within duplex DNA is important for DNA nanotechnology and biotechnology. In this study, the authors investigated the use of iso-2'-deoxyguanosine (iso-dG) as an unnatural base pair. They focused on pseudo-dC (psi dC) as a molecule that recognizes 2-hydroxy-2'-deoxyadenosine (2-OH-dA) in DNA. Through synthesis and analysis, the authors demonstrated that psi dC can form stable base pairs with 2-OH-dA, suggesting its potential applications in DNA manipulation.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Gastroenterology & Hepatology
Ruth X. Wang, Morkos A. Henen, J. Scott Lee, Beat Vogeli, Sean P. Colgan
Summary: The gut microbiota plays a crucial role in human health by providing short-chain fatty acids (SCFAs), particularly butyrate, which contribute to gut homeostasis and disease resistance. Butyrate is shown to directly inhibit hypoxia-inducible factor (HIF) prolyl hydroxylases (PHDs) to stabilize HIF, offering potential therapeutic impact. Microbiota-derived butyrate is essential for stabilizing HIF in murine colonic tissue, suggesting a co-evolution of mammals and mutualistic microbiota in impacting critical gene regulation pathways.
Article
Cell Biology
Yi Fu, Yaqian Huang, Zhao Yang, Yufei Chen, Jingang Zheng, Chenfeng Mao, Zhiqing Li, Zhixin Liu, Bing Yu, Tuoyi Li, Meili Wang, Chanjuan Xu, Yiwei Zhou, Guizhen Zhao, Yiting Jia, Wei Guo, Xin Jia, Tao Zhang, Li Li, Ziyi Liu, Shengchao Guo, Mingliang Ma, Heng Zhang, Bo Liu, Junbao Du, Wengong Wang, Chaoshu Tang, Pei Gao, Qingbo Xu, Xian Wang, Jianfeng Liu, Jinpeng Sun, Wei Kong
Summary: This study reveals that the extracellular matrix protein COMP acts as an endogenous allosteric biased modulator of the AT1 receptor, inhibiting specific signaling pathways through the regulation of intracellular conformational states. COMP deficiency leads to exclusive AAA formation in response to AngII infusion, indicating a potential therapeutic target for cardiovascular diseases.
Article
Multidisciplinary Sciences
Yao Li, Zi-Yu Liang, Hai -Lin Wang
Summary: 6mdA is identified as an agonist that promotes the hyperproliferation of BFU-E progenitor cells and represses the apoptosis of erythroid progenitor cells. Combined use of with SCF and EPO enables cultures of isolated BFU-E to be expanded up to 5,000-fold, providing a new approach for ex vivo generation of red blood cells.
Article
Biochemistry & Molecular Biology
Izabela K. Sibilska-Kaminski, Adrian Fabisiak, Pawel Brzeminski, Lori A. Plum, Rafal R. Sicinski, Hector F. DeLuca
Summary: A series of highly biologically active analogs of (20S,22R)-1 alpha,25-dihydroxy-22-methyl-2-methylene-vitamin D-3, with different side chains, have been efficiently synthesized as potential medical therapy agents. The design of these compounds was based on literature analysis and molecular docking experiments. The synthesized compounds showed high in vitro potency and exhibited significant calcemic activity on bone, especially in the intestinal calcium transport.
BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Joanna M. Swarbrick, Andrew M. Riley, Stephen J. Mills, Barry V. L. Potter
BIOCHEMICAL SOCIETY TRANSACTIONS
(2015)
Article
Multidisciplinary Sciences
Nikolaos I. Kyratsous, Isabel J. Bauer, Guokun Zhang, Marija Pesic, Ingo Bartholomaeus, Marsilius Mues, Ping Fang, Miriam Woerner, Stephanie Everts, Joachim W. Ellwart, Joanna M. Watt, Barry V. L. Potter, Reinhard Hohlfeld, Hartmut Wekerle, Naoto Kawakami
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Multidisciplinary Sciences
Joanna M. Watt, Richard Graeff, Mark P. Thomas, Barry V. L. Potter
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Dina Vara, Joanna M. Watt, Tiago M. Fortunato, Harry Mellor, Matthew Burgess, Kate Wicks, Kimberly Mace, Shaun Reeksting, Anneke Lubben, Caroline P. D. Wheeler-Jones, Giordano Pula
ANTIOXIDANTS & REDOX SIGNALING
(2018)
Article
Chemistry, Multidisciplinary
Joanna M. Swarbrick, Richard Graeff, Clive Garnham, Mark P. Thomas, Antony Galione, Barry V. L. Potter
CHEMICAL COMMUNICATIONS
(2014)
Article
Biochemistry & Molecular Biology
Merle Nebel, Alexander P. Schwoerer, Dominik Warszta, Cornelia C. Siebrands, Ann-Christin Limbrock, Joanna M. Swarbrick, Ralf Fliegert, Karin Weber, Soeren Bruhn, Martin Hohenegger, Anne Geisler, Lena Herich, Susan Schlegel, Lucie Carrier, Thomas Eschenhagen, Barry V. L. Potter, Heimo Ehmke, Andreas H. Guse
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Chemistry, Medicinal
Christelle Moreau, Tanja Kirchberger, Joanna M. Swarbrick, Stephen J. Bartlett, Ralf Fliegert, Timur Yorgan, Andreas Bauche, Angelika Harneit, Andreas H. Guse, Barry V. L. Potter
JOURNAL OF MEDICINAL CHEMISTRY
(2013)
Article
Chemistry, Medicinal
Joanna M. Swarbrick, Richard Graeff, Hongmin Zhang, Mark P. Thomas, Quan Hao, Barry V. L. Potter
JOURNAL OF MEDICINAL CHEMISTRY
(2014)
Article
Multidisciplinary Sciences
Joanna M. Watt, Mark P. Thomas, Barry V. L. Potter
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Bo Zhang, Joanna M. Watt, Chiara Cordiglieri, Werner Dammermann, Mary F. Mahon, Alexander Fluegel, Andreas H. Guse, Barry V. L. Potter
SCIENTIFIC REPORTS
(2018)
Article
Chemistry, Multidisciplinary
Xiangdong Su, Wolfgang Dohle, Stephen J. Mills, Joanna M. Watt, Ana M. Rossi, Colin W. Taylor, Barry V. L. Potter
Article
Chemistry, Multidisciplinary
Ondrej Baszczynski, Joanna M. Watt, Monika D. Rozewitz, Ralf Fliegert, Andreas H. Guse, Barry V. L. Potter
Article
Multidisciplinary Sciences
Frank J. P. Kuehn, Joanna M. Watt, Barry V. L. Potter, Andreas Luckhoff
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Ralf Fliegert, Joanna M. Watt, Anja Schoebel, Monika D. Rozewitz, Christelle Moreau, Tanja Kirchberger, Mark P. Thomas, Wiebke Sick, Andrea C. Araujo, Angelika Harneit, Barry V. L. Potter, Andreas H. Guse
BIOCHEMICAL JOURNAL
(2017)