Journal
NANO RESEARCH
Volume 11, Issue 5, Pages 2532-2543Publisher
TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-017-1878-7
Keywords
vertical silicon nanostructures; metal-assisted chemical etching; primary hippocampal neurons; neurite outgrowth; cytoskeletal dynamics
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Funding
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [NRF-2013R1A1A107-6103, NRF-2012R1A3A2026403, NRF-2012-000-9664]
- GIST Research Institute (GRI)
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In this study, we report that height-controlled vertically etched silicon nano-column arrays (vSNAs) induce strong growth cone-to-substrate coupling and accelerate In vitroneurite development while preserving the essential features of initial neurite formation. Large-scale preparation of vSNAs with flat head morphology enabled the generation of well-controlled topographical stimulation without cellular impalement. A systematic analysis on topography-induced variations on cellular morphology and cytoskeletal dynamics was conducted. In addition, neurite development on the grid-patterned vSNAs exhibited preferential adhesion to the nanostructured region and outgrowth directionality. The arrangement of cytoskeletal proteins and the expression of a focal adhesion complex indicated that a strong coupling existed between the underlying nanocolumns and growth cones. Furthermore, the height-controlled nanocolumn substrates differentially modulated neurite polarization and elongation. Our findings provide an important insight into neuron-nanotopography interactions and their role in cell adhesion and neurite development.
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