Electrochemical investigation of an interaction of the antidepressant drug aripiprazole with original and damaged calf thymus dsDNA

Interaction of an antipsychotic agent, aripiprazole, with calf thymus double stranded deoxyribonucleic acid, ct-dsDNA, was investigated by differential pulse voltammetry (using two methods; interaction on the dsDNA modified electrode surface and in solution phase) and UV-VIS spectrophotometry. The binding constant between dsDNA and aripiprazole, K, about 3 x 10(5) M (1) was obtained spectrophotometrically in 0.1 M acetate buffer, at pH 4.7. Moreover, the DNA-drug association interaction was confirmed by the differential pulse (DP) voltammetric and spectrophotometric investigations of the systems aripiprazole - polyGuanine (polyG) and aripiprazole - polyAdenine (polyA) in solution phase. The interaction between aripiprazole and damaged ct-dsDNA was also investigated using differential pulse voltammetry. (C) 2015 Elsevier Ltd. All rights reserved.