Article
Agronomy
Long Zhang, Yanzhou Qi, Mingming Wu, Lei Zhao, Zhichao Zhao, Cailin Lei, Yuanyuan Hao, Xiaowen Yu, Yinglun Sun, Xin Zhang, Xiuping Guo, Yulong Ren, Jianmin Wan
Summary: This study identified the P-type PPR protein PPR5 in rice as crucial for mitochondrial function and endosperm development, by controlling the cis-splicing of mitochondrial nad4 intron 3. Loss of PPR5 function resulted in decreased ATP production, upregulation of alternative oxidases expression, and abnormal mitochondrial morphology, impacting starch and protein content in the endosperm.
Article
Biochemistry & Molecular Biology
Jing Yang, Yang Cui, Xiangbo Zhang, Zhijia Yang, Jinsheng Lai, Weibin Song, Jingang Liang, Xinhai Li
Summary: PPR278 protein plays a regulatory role in maize seed development, involving in intron splicing and RNA editing in mitochondria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Kaijian Fan, Zhenjing Ren, Xiaofeng Zhang, Yan Liu, Junjie Fu, Chunlai Qi, Wurinile Tatar, Allan G. Rasmusson, Guoying Wang, Yunjun Liu
Summary: In this study, a nuclear-encoded mitochondrial P-type pentatricopeptide repeat (PPR) protein, EMP603, was characterized to play a crucial role in intron splicing in plant mitochondria. Interactions between EMP603 and other proteins indicate the involvement of a dynamic 'spliceosome-like' complex in intron splicing and offer insights into the mechanism of intron splicing in plant mitochondria. Defects in EMP603 disrupt the assembly and activity of mitochondrial complex I, leading to impaired mitochondrial function and delayed seed development.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Dalin Yang, Shi-Kai Cao, Huanhuan Yang, Rui Liu, Feng Sun, Le Wang, Miaodi Wang, Bao-Cai Tan
Summary: In maize, the mitochondrion-localized DYW-type PPR protein DEK48 plays an important role in C-to-U RNA editing. Loss of function of DEK48 abolishes editing at multiple mitochondrial transcripts, resulting in defective seed development and reduced assembly and activity of complex I.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tan-Trung Nguyen, Corinne Best, Sofia Shevtsov, Michal Zmudjak, Martine Quadrado, Ron Mizrahi, Hagit Zer, Hakim Mireau, Oren Ostersetzer-Biran
Summary: Mitochondria play important roles in cellular energy metabolism in eukaryotes, and the expression of angiosperm mtDNA involves multiple RNA-processing steps. Our study reveals a key PPR protein, MISF2, which is crucial for early embryo development in Arabidopsis. The function of MISF2 protein seems to be conserved between different species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zheyuan Wang, Weiwei Chen, Song Zhang, Jiawen Lu, Rongrong Chen, Junjie Fu, Riliang Gu, Guoying Wang, Jianhua Wang, Yu Cui
Summary: In maize, the Dek504 gene plays a crucial role in RNA editing. The PPR protein encoded by Dek504 is required for RNA editing of mitochondrial NADH dehydrogenase 3, which is essential for seed development in maize.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Corinne Best, Ron Mizrahi, Rana Edris, Hui Tang, Hagit Zer, Catherine Colas des Francs-Small, Omri M. Finkel, Hongliang Zhu, Ian D. Small, Oren Ostersetzer-Biran
Summary: Mitochondrial biogenesis relies on nuclearly encoded factors, which regulate the expression of the organellar-encoded genes. Pentatricopeptide repeat (PPR) proteins constitute a major gene family in angiosperms that are pivotal in many aspects of mitochondrial (mt)RNA metabolism (e.g. trimming, splicing, or stability). The loss-of-function allele of the MSP1 gene leads to seed abortion.
Article
Plant Sciences
Jiao Zhao, Shi-Kai Cao, Xiu-Lan Li, Rui Liu, Feng Sun, Rui-Cheng Jiang, Chunhui Xu, Bao-Cai Tan
Summary: RNA C-to-U editing is important for the expression and function of organellar genes in plants. This study reveals the role of EMP80 in C-to-U editing in maize mitochondria and suggests the potential recruitment of ZmDYW2 as a trans deaminase. Loss of EMP80 function leads to arrested embryogenesis and endosperm development in maize.
Article
Biochemistry & Molecular Biology
Yang Lv, Yueying Wang, Qiang Zhang, Changzhao Chen, Qian Qian, Longbiao Guo
Summary: The study identifies a new protein, WAL3, which plays a crucial role in chloroplast development in rice.
Article
Biochemical Research Methods
Elvis Duran-Sierra, Shuna Cheng, Rodrigo Cuenca, Beena Ahmed, Jim Ji, Vladislav V. Yakovlev, Mathias Martinez, Moustafa Al-Khalil, Hussain Al-Enazi, Javier A. Jo
Summary: Early detection is crucial for improving the survival rate and quality of life for oral cancer patients. However, it is often challenging to distinguish dysplastic and early-stage cancerous oral lesions from benign lesions during standard clinical examination. This study aimed to identify novel biochemical and metabolic autofluorescence biomarkers of oral dysplasia and cancer using multispectral autofluorescence lifetime imaging (maFLIM) endoscopy technologies. Clinical images of benign, precancerous, and cancerous lesions were acquired using a novel maFLIM endoscope, and statistical analyses were performed to identify maFLIM features that can differentiate dysplastic/cancerous from benign oral lesions. Fourteen spectral and time-resolved maFLIM features were found to provide contrast between dysplastic/cancerous and benign oral lesions, representing potential biomarkers of oral dysplasia and cancer. This is the first demonstration of clinical widefield maFLIM endoscopic imaging of biochemical and metabolic autofluorescence biomarkers for early detection of oral cancer.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Plant Sciences
Weiwei Chen, Yu Cui, Zheyuan Wang, Rongrong Chen, Cheng He, Yan Liu, Xuemei Du, Yunjun Liu, Junjie Fu, Guoying Wang, Jianhua Wang, Riliang Gu
Summary: In maize, the maturase-related protein ZmnMAT3 is crucial for seed embryogenesis and the assembly of mitochondrial complex I. Loss of function results in reduced splicing efficiency of introns and abnormal expression of alternative oxidases, indicating its importance in kernel development.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Agronomy
Liang Wang, Wenwei Zhang, Shijia Liu, Yunlu Tian, Xi Liu, Haigang Yan, Yue Cai, Xuan Teng, Hui Dong, Rongbo Chen, Xiaokang Jiang, Yihua Wang, Jianmin Wan
Summary: The fse5 gene encodes a PORR protein essential for splicing of nad4 intron 1, and loss of its function affects seed development and seedling growth.
Article
Plant Sciences
Yanwei Wang, Zhimin Yang, Meng Zhang, Pengfei Ai
Summary: This study identified the crucial role of the rice SSA1 gene in chloroplast biogenesis. SSA1 regulates early chloroplast development and is involved in RNA editing and intron splicing. These findings contribute to the understanding of molecular mechanisms underlying chloroplast generation.
Article
Biochemistry & Molecular Biology
Yan-Zhuo Yang, Shuo Ding, Xin-Yuan Liu, Chunhui Xu, Feng Sun, Bao-Cai Tan
Summary: Mutations in maize DEAD-box RNA helicase 48 (ZmRH48) impair mitochondrial intron splicing, complex biosynthesis, and seed development. ZmRH48 deficiency reduces splicing efficiency of multiple introns, leading to lower levels of mitochondrial complexes and defective kernel formation. ZmRH48 interacts with PPR-SMR1, SPR2, and other proteins to facilitate intron splicing.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Chung-Shien Wu, Shu-Miaw Chaw
Summary: By characterizing mitochondrial and plastid genomes, RNA editing sites, and PPR proteins, we unveiled the evolution of mitochondrial RNA editing in gymnosperms. Our analyses revealed efficient non-synonymous editing and showed an increase in U-ending codons in gymnosperm mitochondria due to synonymous editing. We also found lineage-specific duplications in PLS-class PPR proteins, which likely contribute to accumulated RNA editing sites in gymnosperms.
Article
Biochemistry & Molecular Biology
Yi Zuo, Fan Feng, Weiwei Qi, Rentao Song
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2019)
Article
Plant Sciences
Weiwei Qi, Lei Lu, Shengchan Huang, Rentao Song
Article
Plant Sciences
Guifeng Wang, Wei Fan, Mingyan Ou, Xuewei Wang, Hongli Qin, Fan Feng, Yulong Du, Jiacheng Ni, Jihua Tang, Rentao Song, Gang Wang
Review
Agronomy
Chaobin Li, Rentao Song
THEORETICAL AND APPLIED GENETICS
(2020)
Article
Plant Sciences
Dawei Dai, Lifang Jin, Zhenzhen Huo, Shumei Yan, Zeyang Ma, Weiwei Qi, Rentao Song
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Biochemistry & Molecular Biology
Chaobin Li, Weiwei Qi, Zheng Liang, Xi Yang, Zeyang Ma, Rentao Song
Review
Biochemistry & Molecular Biology
Dawei Dai, Zeyang Ma, Rentao Song
Summary: Recent breakthroughs in transcriptome analysis and gene characterization have provided valuable insights into the genetic control of maize endosperm development. Specific genes and transcriptional regulatory networks have been identified to understand the molecular factors involved in different compartments of endosperm development.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Review
Agronomy
Dawei Dai, Zeyang Ma, Rentao Song
Summary: Maize kernel development is initiated by double fertilization, leading to the formation of a mature kernel comprised of embryo, endosperm, and pericarp. With the release of the maize reference genome and advancements in genomic technologies, there has been a rapid expansion of new knowledge in maize kernel development.
MOLECULAR BREEDING
(2021)
Article
Genetics & Heredity
Yonghui He, Qing Yang, Jun Yang, Yong-Fei Wang, Xiaoliang Sun, Shu Wang, Weiwei Qi, Zeyang Ma, Rentao Song
Summary: The gene ZmYSL2 plays a critical role in mineral transportation during maize seed development, affecting the development and activity of aleurone layer and starchy endosperm cells. Proper functioning of ZmYSL2 and iron accumulation are essential for aleurone cell development, ultimately impacting starch synthesis and degradation processes.
Article
Agronomy
Chongzhi Gong, Shengchan Huang, Rentao Song, Weiwei Qi
Summary: The study compared the CRISPR/Cpf1 system and CRISPR/Cas9 system for multiplex gene editing in maize, finding that the CRISPR/Cpf1 system had lower editing efficiency in the T0 and T1 generations but generated more types of new mutations in the T2 generation. The CRISPR/Cas9 system tended to edit within the on-target range, while the CRISPR/Cpf1 system preferred to edit in between the targets and showed a positive correlation between editing efficiency and Cpf1 expression level. In conclusion, the CRISPR/Cpf1 system offers alternative choices for target-site selection for multiplex gene editing and has acceptable editing efficiency in maize, serving as a valuable alternative choice for gene editing in crops.
Article
Agronomy
Yajing Gu, Xuan Chen, Rentao Song, Weiwei Qi
Summary: A bivector transformation system was developed, which successfully transformed two recalcitrant maize inbred lines. The system shortened the transformation cycle, eliminated interference, and ultimately obtained progenies with the target gene.
Article
Agronomy
Changshuang Jing, Min Wei, Peng Fang, Rentao Song, Weiwei Qi
Summary: A pollen-specific CRISPR/Cas9 system was constructed and successfully generated stable heritable mutants in the next generation, while a conventional ubiquitously expressed CRISPR/Cas9 system caused large numbers of somatic mutations. This approach may be applied in other crops to facilitate plant breeding through germinal cell-specific CRISPR/Cas9 systems.
Article
Plant Sciences
Yang Feng, Yafei Ma, Fan Feng, Xinze Chen, Weiwei Qi, Zeyang Ma, Rentao Song
Summary: This study identified the role of mitochondrial ribosomal protein L10 in the accumulation of nonzein proteins (NZPs) in protein bodies (PBs) in maize endosperm. The interaction between NZP1 and 22 kDa alpha-zein was found to be crucial for the accumulation of NZPs in PBs, providing insights into the mechanisms underlying NZP accumulation. Additionally, comparative proteomic analysis revealed potential applications for foreign protein accumulation in maize PBs.
Article
Biochemistry & Molecular Biology
Qun Wang, Mingmin Wang, Jian Chen, Weiwei Qi, Jinsheng Lai, Zeyang Ma, Rentao Song
Summary: Development of the endosperm differs significantly between monocots and dicots, with persistent endosperm in monocots and transient endosperm in dicots. The mechanisms controlling these differences are not well understood. In this study, a maize mutant, enb1, was characterized, and it was found that the endosperm degradation was drastically increased during kernel development. The ENB1 gene was identified as a key regulator involved in cellulose synthesis and nutrient uptake in the endosperm cells. Additionally, ENB1 expression was found to be induced by sucrose, and overexpression of ENB1 enhanced sucrose transport and contributed to the formation of a persistent endosperm in the kernel.
