Article
Biology
Qing Ma, Liuyi Yang, Karen Tolentino, Guiping Wang, Yang Zhao, Ulrike M. Litzenburger, Quanming Shi, Lin Zhu, Chen Yang, Huiyuan Jiao, Feng Zhang, Rui Li, Miao-Chih Tsai, Jun-An Chen, Ian Lai, Hong Zeng, Lingjie Li, Howard Y. Chang
Summary: This study demonstrates the significant role of human HOTAIR lncRNA in promoting breast cancer progression, including increased metastatic capacity and invasiveness in breast cancer cells, and alterations in the transcriptome and chromatin accessibility landscape.
Article
Oncology
Anuraag S. Parikh, Victoria X. Yu, Samuel Flashner, Ogoegbunam B. Okolo, Chao Lu, Brian S. Henick, Fatemeh Momen-Heravi, Sidharth V. Puram, Theodoros Teknos, Quintin Pan, Hiroshi Nakagawa
Summary: The understanding of tumor heterogeneity has highlighted the importance of 3D culture systems, such as patient derived organoids (PDO) and organotypic culture (OTC), in modeling and studying head and neck squamous cell carcinoma (HNSCC). These models capture the in vivo tumor heterogeneity and can faithfully replicate tumor biology and treatment response, making them valuable tools for research. In vitro 3D culture models serve as a bridge between 2D monolayer culture and in vivo models like patient derived xenografts.
Review
Oncology
Laura Amicone, Alessandra Marchetti, Carla Cicchini
Summary: Epithelial-to-mesenchymal transition (EMT) is a cell differentiation process that gives epithelial cells the ability to move and invade, and its abnormal reactivation in cancer cells is crucial for metastasis. EMT is a dynamic program with various states, and the inverse transition, mesenchymal-to-epithelial transition (MET), is important for colonization of distant sites. Long non-coding RNAs (lncRNAs), specifically HOTAIR, play a critical role in regulating epithelial cell plasticity and EMT in tumors. This review focuses on the molecular mechanisms of HOTAIR expression and its functions in gene regulation and protein activities, as well as the challenges of targeting HOTAIR for therapeutic approaches against EMT.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Health Care Sciences & Services
In-Jai Kim, Jeong-Yong Lee, Hyeon-Woo Park, Han-Sung Park, Eun-Ju Ko, Jung-Hoon Sung, Nam-Keun Kim
Summary: The study suggests that HOTAIR gene polymorphisms may serve as novel biomarkers for diagnosing coronary artery disease (CAD) and are closely associated with CAD susceptibility. Variations in genetic polymorphisms, genotype combinations, and haplotype analysis of HOTAIR are linked to disease occurrence and clinical factors contributing to disease susceptibility.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Food Science & Technology
Pan Chao, Feng Yongheng, Zhou Jin, Zhu Yu, Yang Shiyong, Yang Kunxing, Ma Yong
Summary: This study revealed that in gastric cancer development, HOTAIR expression increases while miRNA-206 expression decreases. Knockdown of HOTAIR suppresses cell viability, increases apoptosis, inhibits invasion and migration by regulating miRNA-206, and suppresses CCND1 and CCND2 expression. HOTAIR targeting miRNA-206 and miRNA-206 targeting CCND1 and CCND2 play crucial roles in the antitumor effects in gastric cancer.
FOOD SCIENCE & NUTRITION
(2021)
Article
Engineering, Biomedical
Naledi Shologu, Mehmet Gurdal, Eva Szegezdi, Una FitzGerald, Dimitrios I. Zeugolis
Summary: This study evaluated the potential of macromolecular crowding in improving cell-derived matrices for in vitro tumor models. NP40 was the most effective decellularisation protocol in removing cellular matter while preserving the deposited matrix. Macromolecular crowding derived matrices produced by mammary fibroblasts showed increased adhesion molecules, metalloproteinases, and proinflammatory cytokines, as well as enhanced resistance to doxorubicin and reduced cell death caused by reactive oxygen species.
Review
Oncology
Vincenza Ylenia Cusenza, Annalisa Tameni, Antonino Neri, Raffaele Frazzi
Summary: Most of our transcribed RNAs are non-coding sequences. Long non-coding RNAs (lncRNAs) are transcripts with no protein coding ability and a length >200nt. They can undergo epigenetic modifications such as m6A, m1A, m5C, m7G, and Nm. The aberrant expression of lncRNAs and epigenetic signatures of m6A- or m5C-related lncRNAs are increasingly recognized as potential prognostic biomarkers in various cancers.
FRONTIERS IN ONCOLOGY
(2023)
Review
Cell Biology
Chunming Zhu, Xia Wang, Yuan Wang, Kefeng Wang
Summary: Chemotherapy is a significant treatment for advanced cancer, but drug-resistant tumors often relapse. The homeobox transcript antisense intergenic RNA (HOTAIR) has been found to play a role in tumor development and is associated with chemotherapy resistance. Therefore, understanding the biology and mechanism of HOTAIR in tumor behavior and its potential as a biomarker for predicting chemotherapy efficacy is crucial.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Julien Jarroux, Dominika Foretek, Claire Bertrand, Marc Gabriel, Ugo Szachnowski, Zohra Saci, Shuling Guo, Arturo Londono-Vallejo, Marina Pinskaya, Antonin Morillon
Summary: The research reveals that the interaction between HOTAIR and LSD1 promotes the migration of epithelial cells and induces partial reprogramming of the epithelial transcriptome by altering LSD1 distribution at promoter and enhancer regions. This uncovers an unexpected role of HOTAIR in EMT as an LSD1 decommissioning factor, counteracting its activity in the control of epithelial identity.
Review
Biochemistry & Molecular Biology
Wencheng Kong, Guang Yin, Sixin Zheng, Xinchun Liu, Akao Zhu, Panpan Yu, Jian Zhang, Yuqiang Shan, Rongchao Ying, Huicheng Jin
Summary: Gastric cancer is one of the most common malignant tumors in the world, with many genetic and epigenetic risk factors playing a significant role in its development. HOTAIR, as an important epigenetic factor, is involved in the development of gastric cancer through various mechanisms and has prognostic value.
Review
Oncology
Xiaoru Xin, Qianan Li, Jinyong Fang, Tiejun Zhao
Summary: Long non-coding RNAs are crucial regulators of gene expression and physiological processes, with HOX transcript antisense RNA (HOTAIR) being an oncogenic lncRNA highly upregulated in various human cancers. Targeting HOTAIR shows therapeutic potential in cancer treatment, with studies focusing on drug resistance, survival, and potential therapies. Preclinical studies are needed to further explore molecular mechanisms underlying HOTAIR dysregulation and efficient drug delivery methods in vivo.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, Research & Experimental
Ruifeng Pei, Le Zhao, Yiren Ding, Zhan Su, Deqiang Li, Shuo Zhu, Lu Xu, Wei Zhao, Wuyuan Zhou
Summary: This study reveals the promoting effect of HOTAIR in radioresistance of liver cancer stem cells (LCSCs). Mechanistically, HOTAIR recruits LSD1 to the MAPK1 promoter region, reduces the level of H3K9me2, and elevates ERK2 (MAPK1) expression. The JMJD6-BRD4 complex positively regulates HOTAIR transcription, ERK2 (MAPK1) expression, and maintains the stemness of LCSCs, ultimately promoting their radioresistance.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Endocrinology & Metabolism
Riddhi K. Pandya, Shubhashree Uppangala, Sujith R. Salian, Sanjay Gupta, Guruprasad Kalthur, Stefan Schlatt, Satish Kumar Adiga
Summary: This study investigated the impact of hypothermic holding on DNA methylation in mouse immature-testicular-tissue (ITT). The results showed that short-term holding at 4 degrees C did not affect DNA methylation after organotypic culture.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Geriatrics & Gerontology
Quanzhi Liang, Ailijiang Asila, Yingjie Deng, Jun Liao, Zhenfeng Liu, Rui Fang
Summary: Osteopontin-induced HOTAIR expression is involved in osteoarthritis by regulating cell proliferation. The study found that HOTAIR was upregulated in OA and showed a positive correlation with osteopontin. While osteopontin treatment increased HOTAIR expression in primary chondrocytes, HOTAIR overexpression and knockdown did not significantly affect osteopontin expression. Additionally, both osteopontin and HOTAIR overexpression increased chondrocyte proliferation, while HOTAIR knockdown decreased chondrocyte proliferation and reduced the effects of osteopontin treatment on cell proliferation.
Article
Cell Biology
Yan-Ping Guo, Zhi-Feng Wang, Na Li, Qian-Qian Lei, Qiong Cheng, Li-Gang Shi, Sheng-Li Zhou, Xin-Hua Wang, Yang Sun, Ling-Fei Kong
Summary: This study found that lncRNA HOTAIR regulates RCC angiogenesis through the miR-126/EGFL7 axis, providing a new perspective on the molecular pathways of angiogenesis in RCC development and potentially serving as therapeutic targets for RCC treatment.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Oncology
Andra S. Martinikova, Miroslav Stoyanov, Anna Oravetzova, Yannick P. Kok, Shibo Yu, Jana Dobrovolna, Pavel Janscak, Marcel van Vugt, Libor Macurek
Summary: Oncogene-induced replication stress is a major cause of genome instability in cancer cells. This study reveals that increased activity of PPM1D exacerbates replication stress caused by cyclin E1 overexpression, leading to abnormal cell cycle progression and accumulation of DNA copy number alterations. Pharmacological inhibition of PPM1D can alleviate replication stress-induced genome instability.
MOLECULAR ONCOLOGY
(2024)
Article
Oncology
Alamelu G. Bharadwaj, Meghan E. McLean, Margaret L. Dahn, Hannah F. Cahill, Marie-Claire D. Wasson, Raj Pranap Arun, Olivia L. Walker, Brianne M. Cruickshank, Wasundara Fernando, Jaganathan Venkatesh, Penelope J. Barnes, Gillian Bethune, Gregory Knapp, Lucy K. Helyer, Carman A. Giacomantonio, David M. Waisman, Paola Marcato
Summary: ALDH1A3 regulates the plasminogen activation pathway to promote breast cancer metastasis. Co-expression of ALDH1A3 and tPA is associated with TNBC subtype, high tumor grade, and recurrent metastatic disease.
MOLECULAR ONCOLOGY
(2024)
Article
Oncology
Nayela N. Chowdhury, Yi Yang, Ananya Dutta, Michelle Luo, Zimu Wei, Sara R. Abrahams, Alexey S. Revenko, Fenil Shah, Lindsey A. Miles, Robert J. Parmer, Bas de Laat, Alisa S. Wolberg, James P. Luyendyk, Melissa L. Fishel, Matthew J. Flick
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a highly fatal metastatic disease associated with robust activation of the coagulation and fibrinolytic systems. Primary fibrinolytic protease plasminogen promotes PDAC tumor growth and metastatic potential through engaging plasminogen receptors on tumor cells.
MOLECULAR ONCOLOGY
(2024)
Article
Oncology
Nuria Gendrau-Sanclemente, Agnes Figueras, Kristina Gracova, Alvaro Lahiguera, Elisenda Alsina-Sanchis, Juan A. Marin-Jimenez, August Vidal, Xavier Matias-Guiu, Sergi Fernandez-Gonzalez, Marc Barahona, Lola Marti, Jordi Ponce, Francesc Vinals
Summary: High-grade serous ovarian cancer (HGSOC), the deadliest gynecological malignancy, spreads through transcoelomic dissemination. This study reveals that platelet-derived growth factor receptor beta (PDGFRβ) is essential for the formation of tumorspheres in HGSOC. Inhibition of PDGFRβ blocks the clustering of ovarian cancer cells and prevents peritoneal dissemination.
MOLECULAR ONCOLOGY
(2024)