Article
Virology
Genevieve F. Oliver, Liam M. Ashander, Abby C. Dawson, Yuefang Ma, Jillian M. Carr, Keryn A. Williams, Justine R. Smith
Summary: Retinopathy is a recognized complication of dengue, affecting hospitalized patients up to 10%. This study aimed to investigate the susceptibility of Muller cells to dengue virus (DENV) infection and their antiviral, inflammatory, and immunomodulatory responses in vitro. It was found that Muller cells can be infected by DENV and mount an immune response, but the response varies across different cell isolates and DENV strains. This research provides insights into the role of retinal Muller glial cells in dengue retinopathy.
Article
Oncology
Han Koo, Sangwon Byun, Jieun Seo, Yuri Jung, Dong Chul Lee, Jung Hee Cho, Young Soo Park, Young Il Yeom, Kyung Chan Park
Summary: IGF-1R is commonly overexpressed in solid tumors, but gene amplification is rare. Research shows that PKM2 regulates IGF-1R protein expression by stabilizing the precursor protein through HSP90 binding. Inhibiting PKM2 reduces IGF-1R levels, inhibits AKT activation, and increases apoptotic cell death, suggesting PKM2 as a potential therapeutic target in cancers with dysregulated IGF signaling.
Article
Multidisciplinary Sciences
Virginia Actis Dato, Maria Cecilia Sanchez, Gustavo Alberto Chiabrando
Summary: IGF-1 regulates GLUT1 translocation and glucose uptake in MGCs through activation of MAPK/ERK and PI3K/Akt pathways, with a molecular association between LRP1 and GLUT1 being significantly reduced by IGF-1. Specific siRNA targeting LRP1 results in decreased GLUT1 expression on the plasma membrane and impaired glucose uptake induced by IGF-1.
SCIENTIFIC REPORTS
(2021)
Article
Ophthalmology
Esma Yerlikaya, Allyson L. Toro, Siddharth Sunilkumar, Ashley M. VanCleave, Ming Leung, Yuka Imamura Kawasawa, Scot R. Kimball, Michael D. Dennis
Summary: In diabetic retinopathy, neuroglial dysfunction occurs early and Muller glia play a significant role in disease progression. This study investigates the common signaling pathways activated in Muller glia by both type 1 and pre-/type 2 diabetes. The findings suggest that the TREM2/DAP12 receptor-adaptor complex signals via SYK to promote HIF1a stabilization and increased angiogenic cytokine production by Muller glia.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Review
Pathology
Jason Y. Zhang, Mark J. Greenwald, Sarah H. Rodriguez
Summary: Retinopathy of prematurity (ROP) is strongly associated with gestational age and weight at birth, but not all extremely preterm infants develop ROP. Dysbiosis of the gut microbiome may play a role in the development of ROP, along with other known risk factors. This mini-review provides an overview of these proposed mechanisms, establishing a foundation for future studies.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Cell Biology
Paula V. Subirada, Maria V. Vaglienti, Mariana B. Joray, Maria C. Paz, Pablo F. Barcelona, Maria C. Sanchez
Summary: This study investigated the changes in autophagy flux during hypoxia and hypoxia-reoxygenation, as well as the effects of Rapamycin and Resveratrol on the gliotic and pro-angiogenic response of Muller glial cells (MGCs) under hypoxic conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Pei-Chin Chen, Yung-Che Kuo, Cheng-Ming Chuong, Yen-Hua Huang
Summary: Stem cells interact harmoniously with their niches during development and tumor pathology, influencing cell fate. The modulation of IGF-1R signaling plays a crucial role in determining cell fate.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Aude Couturier, Guillaume Blot, Lucile Vignaud, Celine Nanteau, Amelie Slembrouck-Brec, Valerie Fradot, Niyazi Acar, Jose-Alain Sahel, Ramin Tadayoni, Gilles Thuret, Florian Sennlaub, Jerome E. Roger, Olivier Goureau, Xavier Guillonneau, Sacha Reichman
Summary: Muller glial cells play a crucial role in maintaining retinal homeostasis and metabolism, but reliable human cell sources for modeling MGC-associated diseases are limited. The newly developed hiMGCs exhibit phenotypic and transcriptomic similarities to pMGCs, making them valuable tools for disease modeling, such as diabetic retinopathy.
Article
Medicine, Research & Experimental
Jing Zhang, Yaowu Qin, Mireya Martinez, Miguel Flores-Bellver, Murilo Rodrigues, Aumreetam Dinabandhu, Xuan Cao, Monika Deshpande, Yu Qin, Silvia Aparicio-Domingo, Yuan Rui, Stephany Y. Tzeng, Shaima Salman, Jin Yuan, Adrienne W. Scott, Jordan J. Green, M. Valeria Canto-Soler, Gregg L. Semenza, Silvia Montaner, Akrit Sodhi
Summary: Therapies targeting VEGF have limited effectiveness for treating proliferative sickle cell retinopathy (PSR), the leading cause of blindness in sickle cell disease patients. Increased expression of HIF-1 alpha and HIF-2 alpha in the ischemic inner retina of PSR eyes was observed. While both HIFs promote VEGF expression, only HIF-1 is sufficient to promote retinal neovascularization in mice. Inhibiting either HIF-1 or HIF-2 is effective for preventing retinal neovascularization in mice, but targeting both will be necessary for preventing NV in patients with PSR.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cell Biology
Heng-Kien Au, Syue-Wei Peng, Chin-Lin Guo, Chien-Chia Lin, Yi-Lin Wang, Yung-Che Kuo, Tsz-Yau Law, Hong-Nerng Ho, Thai-Yen Ling, Yen-Hua Huang
Summary: The study revealed the synergistic effect of ECM laminin, hypoxia, and insulin-like growth factor 1 receptor (IGF-1R) in maintaining the self-renewal ability of embryonic germline stem cells. Through regulating specific signaling pathways, the expression of stemness-related genes was maintained, promoting cell proliferation and migration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Robin M. Perelli, Matthew L. O'Sullivan, Samantha Zarnick, Jeremy N. Kay
Summary: The exposure of excess oxygen in neonatal mice disrupts the formation of astrocyte template, leading to their excessive proliferation and interference with retinal angiogenesis upon return to normal oxygen levels. This dysregulation of astrocyte proliferation may contribute to vascular defects resembling ROP.
Article
Biotechnology & Applied Microbiology
Na Zhao, Xiao Gui, Qian Fang, Rui Zhang, Weiye Zhu, Haorui Zhang, Qing Li, Yukun Zhou, Jiawei Zhao, Xiao Cui, Guangping Gao, Huipeng Tang, Ni Shen, Taoyong Chen, Hongyuan Song, Wei Shen
Summary: The study found that Graphene Quantum Dots (GQDs) are biocompatible nanomaterials that can inhibit proliferation, migration, tube formation, and sprouting of human umbilical vein endothelial cells in a dose-dependent manner, and can inhibit pathological retinal neovascularization in an oxygen-induced retinopathy model. The data suggest that GQDs disrupt the periostin/ERK pathway and subsequent cell cycle regulation. This signaling pathway was confirmed in an OIR mouse model.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Bo Chen, Shunhui Wei, See Wee Low, Charlene Priscilla Poore, Andy Thiam-Huat Lee, Bernd Nilius, Ping Liao
Summary: Reperfusion therapy for acute ischemic stroke aims to restore blood flow but can cause reperfusion injury. Delayed recanalization increases the risk of severe injury. The TRPM4-blocking antibody M4P has been shown to alleviate reperfusion injury and improve outcomes. In this study, M4P treatment improved vascular morphology, reduced leakage, and improved cerebral perfusion, resulting in reduced infarct volume and mortality rate in a delayed stroke reperfusion rat model.
Article
Cell Biology
Minqi Zhu, Na Li, Yanuo Wang, Shuang Gao, Jing Wang, Xi Shen
Summary: Inflammatory changes play a crucial role in the development of diabetic retinopathy (DR). This study explores the relationship between VEGF and BDNF in mouse retinal MGCs during inflammation in diabetes and suggests that VEGF may regulate cytokines through the BDNF/NF-κB signaling pathway. These findings provide important insights for therapeutic strategies for DR.
CELL AND TISSUE RESEARCH
(2022)
Article
Oncology
Wei Xie, Huijie Zhao, Fengxian Wang, Yiyun Wang, Yuan He, Tong Wang, Kunchi Zhang, Hao Yang, Zhaoli Zhou, Haibin Shi, Jin Wang, Gang Huang
Summary: A novel humanized antibody targeting Fzd7, named SHH002-hu1, was developed and demonstrated potential anti-tumor activity. It showed high affinity with Fzd7, specifically targeted Fzd7(+) cells and tumor tissues, and enhanced the anti-TNBC capacity of Bevacizumab through inhibiting Wnt/beta-catenin signaling. SHH002-hu1 could be a promising candidate for synergistic therapy with Bevacizumab in preventing TNBC recurrence.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Dario G. Ferrer, Virginia Actis Dato, Javier R. Jaldin Fincati, Valeria E. Lorenc, Maria C. Sanchez, Gustavo A. Chiabrando
JOURNAL OF CELLULAR BIOCHEMISTRY
(2017)
Review
Neurosciences
Paula V. Subirada, Maria C. Paz, Magali E. Ridano, Valeria E. Lorenc, Maria V. Vaglienti, Pablo F. Barcelona, Jose D. Luna, Maria C. Sanchez
EUROPEAN JOURNAL OF NEUROSCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Ruben Adrian Grosso, Paula Virginia Subirada Caldarone, Maria Cecilia Sanchez, Gustavo Alberto Chiabrando, Maria Isabel Colombo, Claudio Marcelo Fader
BIOSCIENCE REPORTS
(2019)
Article
Neurosciences
Paula V. Subirada, Maria C. Paz, Magali E. Ridano, Valeria E. Lorenc, Claudio M. Fader, Gustavo A. Chiabrando, Maria C. Sanchez
FRONTIERS IN CELLULAR NEUROSCIENCE
(2019)
Article
Multidisciplinary Sciences
Javier R. Jaldin-Fincati, Virginia Actis Dato, Nicolas M. Diaz, Maria C. Sanchez, Pablo F. Barcelona, Gustavo A. Chiabrando
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Maria C. Paz, Pablo F. Barcelona, Paula V. Subirada, Magali E. Ridano, Gustavo A. Chiabrando, Claudia Castro, Maria C. Sanchez
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Ophthalmology
Marilyn A. Marquez, Claudio P. Juarez, Maria C. Sanchez, Jose D. Luna
Summary: This case report describes a unique presentation in a patient with NF1, rubeosis iridis, high IOP, and macular hemorrhages from multiple corkscrew retinal vessels in a well-perfused retina. Treatment with anti-angiogenics led to a good visual outcome.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Multidisciplinary Sciences
Virginia Actis Dato, Maria Cecilia Sanchez, Gustavo Alberto Chiabrando
Summary: IGF-1 regulates GLUT1 translocation and glucose uptake in MGCs through activation of MAPK/ERK and PI3K/Akt pathways, with a molecular association between LRP1 and GLUT1 being significantly reduced by IGF-1. Specific siRNA targeting LRP1 results in decreased GLUT1 expression on the plasma membrane and impaired glucose uptake induced by IGF-1.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Paula Subirada, Maria C. Paz, Maria V. Vaglienti, Jose D. Luna, Pablo F. Barcelona, Maria C. Sanchez
Summary: Retinopathies are a group of diseases that affect the neurosensory tissue of the eye, leading to neurodegeneration and changes in vascular function and structure. By using animal models and image processing, we can measure retinal vascular parameters such as density, diameter, tortuosity, and neovascular areas. These tools are highly helpful in evaluating and quantifying vascular alterations in retinopathies.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cell Biology
Paula V. Subirada, Maria V. Vaglienti, Mariana B. Joray, Maria C. Paz, Pablo F. Barcelona, Maria C. Sanchez
Summary: This study investigated the changes in autophagy flux during hypoxia and hypoxia-reoxygenation, as well as the effects of Rapamycin and Resveratrol on the gliotic and pro-angiogenic response of Muller glial cells (MGCs) under hypoxic conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Maria Florencia Soler, Rocio del Carmen Bravo-Miana, Dana Maria Negretti-Borga, Paula Subirada, Vanina Alejandra Alamino, Maria Cecilia Sanchez, Ana Carolina Donadio, Claudia Gabriela Pellizas, Maria del Mar Montesinos
Summary: T3-conditioned dendritic cell vaccine shows effective anti-tumor effects in a murine model of colon carcinoma, enhancing immune response by increasing CD86 expression, promoting the production of inflammatory factors, and inducing specific cytotoxic T lymphocytes. T3 also promotes the maturation and activation of human dendritic cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Cell Biology
Paula Virginia Subirada, Albana Tovo, Maria Victoria Vaglienti, Jose Domingo Luna Pinto, Horacio Uri Saragovi, Maria Cecilia Sanchez, Agustin Anastasia, Pablo Federico Barcelona
Summary: Choroidal neovascularization (CNV) is a pathological angiogenesis of the choroidal plexus of the retina and is a key feature in the wet form of age-related macular degeneration. Mononuclear phagocytic cells (MPCs) are recruited and activated through p75 neurotrophin receptor (p75(NTR)) in a mouse model of laser-induced CNV, contributing to CNV pathology. Knockout of p75(NTR) or treatment with its antagonist reduces MPC recruitment, glial activation, CNV area, and preserves retinal function in mice with CNV.
Article
Cell Biology
Maria V. Vaglienti, Paula V. Subirada, Mariana B. Joray, Gustavo Bonacci, Maria C. Sanchez
Summary: Inflammation, oxidative stress, and nitrosative stress are involved in the pathogenesis of proliferative retinopathies. Nitro-oleic acid (NO2-OA) shows a protective effect on oxidative damage, gliosis, and the pro-angiogenic response in Muller glial cells.
Review
Neurosciences
Maria C. Sanchez, Gustavo A. Chiabrando
Summary: Muller glial cells (MGCs) play an important role in retinal homeostasis and the development of proliferative retinopathies, while low-density lipoprotein receptor-related protein 1 (LRP1) has been identified as a key player in these processes. Further research in this area could lead to novel therapeutic approaches.