Article
Biochemistry & Molecular Biology
Yushi Abe, Daigo Ochiai, Masako Taguchi, Seiji Kanzaki, Satoru Ikenoue, Yoshifumi Kasuga, Mamoru Tanaka
Summary: Human amniotic fluid stem cells (hAFSCs) can form aggregates with host immune cells and alleviate inflammation. Additionally, hAFSCs can increase regulatory T cells in the acquired immune system. Therefore, hAFSCs play an important role in the treatment of inflammation-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Giedre Valiuliene, Aiste Zentelyte, Elizabet Berzanskyte, Ruta Navakauskiene
Summary: hAFSCs derived from pathological gestations with polyhydramnios exhibit differences in metabolic and inflammatory status compared to hAFSCs from normal gestations, as well as possessing lower neurogenic potential. Further studies are necessary to explore the potential of hAFSCs from polyhydramnios in stem cell-based therapies and strategies to enhance their characteristics for regenerative medicine purposes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kantirat Yaja, Sirinda Aungsuchawan, Suteera Narakornsak, Peraphan Pothacharoen, Rungusa Pantan, Waleephan Tancharoen
Summary: This study compared the effectiveness and safety of different culture supplements (FBS or hPL) on the osteoblastic differentiation potential of hAF-MSCs under a three-dimensional gelatin scaffold. The results showed that hAF-MSCs have the potential to be used in clinical applications and hPL as a supplement significantly stimulated osteoblast differentiation and had better gene expression and cell functionality compared to FBS.
Article
Multidisciplinary Sciences
Jun Jie Tan, Jacques P. Guyette, Kenji Miki, Ling Xiao, Gurbani Kaur, Tong Wu, Liye Zhu, Katrina J. Hansen, King-Hwa Ling, David J. Milan, Harald C. Ott
Summary: The study demonstrates the generation of premature PECs using BVR stimulation, co-culturing PECs with cardiomyocytes leads to cardiomyocyte aggregation, proliferation and the formation of more mature structures with improved beating/contractility.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Chunhui Sheng, Xiaodong Guo, Zhuqing Wan, Xiaoqiang Bai, Hao Liu, Xiao Zhang, Ping Zhang, Yunsong Liu, Wenyue Li, Yongsheng Zhou, Longwei Lv
Summary: This study investigated the potential of exosomes derived from human adipose-derived stem cells (hASCs) in promoting osteogenic differentiation for osteoporosis treatment. The study identified the key differentially expressed microRNA between two exosomes and their potential target gene. Results showed that exosomes from osteogenic induction medium had a better effect on promoting osteogenic differentiation than those from proliferation medium. Moreover, miR-335-3p inhibitor-optimized exosomes enhanced osteogenic differentiation in vitro and bone density in vivo, suggesting the potential of engineered exosomes in optimizing exosome-based therapy for osteoporosis.
Article
Biotechnology & Applied Microbiology
Aiste Zentelyte, Deimante Zukauskaite, Ieva Jaceryte, Veronika V. Borutinskaite, Ruta Navakauskiene
Summary: In this study, short-term treatments of small molecules were used to enhance the properties and differentiation capability of human amniotic fluid stem cells (AFSC). The combinations of epigenetically active compounds and multifunctional molecules upregulated genes involved in pluripotency, leading to a more energetically active phenotype in AFSCs. The small molecule treatments also showed a beneficial effect on differentiation potential towards a neurogenic lineage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Xiao Xu, Yujie Liang, Xingfu Li, Kan Ouyang, Manyi Wang, Tong Cao, Wencui Li, Jianquan Liu, Jianyi Xiong, Biquan Li, Jiang Xia, Daping Wang, Li Duan
Summary: Targeted delivery of Kartogenin (KGN) to synovial fluid-derived mesenchymal stem cells (SF-MSCs) by engineered exosomes increases its effective concentration in the cell, enhancing chondrogenesis in vitro and in vivo.
Article
Pharmacology & Pharmacy
Ying Xing, Mi-Shuai Zhang, Jian-Hui Xiao, Ru-Ming Liu
Summary: This study found that galangin can promote the osteogenic differentiation of human amniotic mesenchymal stem cells (hAMSCs) through the JAK2/STAT3 signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Cell & Tissue Engineering
Shune Xiao, Chunfang Xiao, Yong Miao, Jin Wang, Ruosi Chen, Zhexiang Fan, Zhiqi Hu
Summary: The study demonstrated that hAAM scaffolds incorporated with ADSC-Exos can promote diabetic skin wound healing by regulating inflammation, stimulating vascularization, and promoting the production of extracellular matrix.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Massimo Conese, Ottavio Napolitano, Onofrio Laselva, Sante Di Gioia, Luigi Nappi, Luigia Trabace, Maria Matteo
Summary: This study found that the levels of PLAC1 in AMSCs were lower in patients with preeclampsia, and abnormal shedding of PLAC1 from AMSC plasma membranes may contribute to trophoblast proliferation and support its role in the development of preeclampsia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Francesca Santilli, Jessica Fabrizi, Stefano Martellucci, Costantino Santacroce, Egidio Iorio, Maria Elena Pisanu, Mattea Chirico, Loreto Lancia, Fanny Pulcini, Valeria Manganelli, Maurizio Sorice, Simona Delle Monache, Vincenzo Mattei
Summary: This study analyzed the lipid composition of human Dental Pulp-Derived Stem Cells (DPSCs) and investigated the role of lipid rafts during multilineage differentiation. The results showed that mono-unsaturated fatty acids were the most representative species in the acyl chains of DPSCs. Moreover, disrupting lipid rafts prevented AKT activation and lineage-specific mRNA expression during multilineage differentiation, indicating the importance of lipid raft integrity in DPSCs differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Medicine, General & Internal
Han Luo, Zhen Wang, Fang Qi, Dali Wang
Summary: Complete wound regeneration is crucial for maintaining skin structure and physiological functions, and amniotic fluid stem cells (AFSCs) have the potential to play a significant role in wound generation and treatment. AFSCs are easily accessible and have advantages such as anti-inflammatory and immunomodulatory properties, which can promote full wound regeneration.
CHINESE MEDICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Stefanska, Lucie Nemcova, Malgorzata Blatkiewicz, Wojciech Pienkowski, Marcin Rucinski, Maciej Zabel, Paul Mozdziak, Marzenna Podhorska-Okolow, Piotr Dziegiel, Bartosz Kempisty
Summary: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) possess multilineage differentiation potential and specific surface markers. In this study, RNA sequencing analysis revealed differentially expressed genes associated with apoptosis and identified upregulation of ZBTB16 and FOXO1, as well as downregulation of TGFA, in all differentiated groups. Additionally, several novel marker genes related to WJ-MSCs differentiation were identified. This study sheds light on the molecular mechanisms involved in long-term culture and differentiation of WJ-MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Amany Solaiman, Radwa A. Mehanna, Ghada A. Meheissen, Soha Elatrebi, Rasha Said, Nahed H. Elsokkary
Summary: Amniotic fluid-mesenchymal stem cells have shown promising protective and therapeutic effects against hyperoxia-induced lung fibrosis, as evaluated in terms of physiology, histology, and biochemistry.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Yushi Abe, Daigo Ochiai, Seiji Kanzaki, Yu Sato, Toshimitsu Otani, Satoru Ikenoue, Yoshifumi Kasuga, Mamoru Tanaka
Summary: This study demonstrates that hAFSCs are effective in the treatment and prevention of inflammation-induced preterm birth. hAFSCs suppress inflammation and reduce preterm birth by switching macrophage polarity.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Integrative & Complementary Medicine
Sasimol Udomruk, Chayanut Kaewmool, Thanyaluck Phitak, Peraphan Pothacharoen, Prachya Kongtawelert
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2020)
Article
Immunology
Chayanut Kaewmool, Prachya Kongtawelert, Thanyaluck Phitak, Peraphan Pothacharoen, Sasimol Udomruk
JOURNAL OF NEUROIMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Hathaichanok Chuntakaruk, Prachya Kongtawelert, Peraphan Pothacharoen
Summary: Purple corn anthocyanins have shown to be beneficial in reducing inflammation associated with diabetes and may help ameliorate cartilage degradation. By inhibiting the NF kappa b and MAPK signaling pathways, purple corn anthocyanins and the metabolite PCA exhibit anti-inflammatory effects on AGEs-induced cartilage degradation.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Aitthiphon Chongchai, Sajee Waramit, Keittisak Suwan, Mariam Al-Bahrani, Sasimol Udomruk, Thanyaluck Phitak, Prachya Kongtawelert, Peraphan Pothacharoen, Amin Hajitou
Summary: A targeted chondrosarcoma gene therapy using engineered phage particles showed promising, selective, and efficient delivery of therapeutic genes to chondrosarcoma cells. In vitro studies demonstrated significant cell killing and high expression of TNF alpha and apoptosis-related genes when treated with the targeted particles. In vivo experiments on mice with chondrosarcoma further supported the potential of this phage-based therapy for chondrosarcoma.
Article
Neurosciences
Sasimol Udomruk, Benjawan Wudtiwai, Thuzar Hla Shwe, Thanyaluck Phitak, Peraphan Pothacharoen, Mattabhorn Phimphilai, Prachya Kongtawelert
Summary: Neuroinflammation-mediated microglial reactivity plays a crucial role in the increased risk of Alzheimer's disease development in patients with Type 2 diabetes mellitus. Advanced glycation end products (AGEs) formed in diabetes are intermediaries of brain injury. Sesamin, a lignan found in sesame seed oils, shows promise in delaying inflammatory progress by inhibiting AGEs-induced microglial reactivity.
BRAIN RESEARCH BULLETIN
(2021)
Article
Biochemistry & Molecular Biology
Thuzar Hla Shwe, Peraphan Pothacharoen, Thanyaluck Phitak, Benjawan Wudtiwai, Prachya Kongtawelert
Summary: In this study, the synergistic upregulation of PD-L1 expression in HepG2 cells by a combination of IFNγ and TNFα was observed, with STAT1 activation playing a major role in this synergistic effect. Atorvastatin was found to inhibit the induction of PD-L1 by either IFNγ alone or IFNγ/TNFα combination treatment in HepG2 cells. These findings suggest that atorvastatin may impact tumor immune response through inhibiting PD-L1 induction in cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Benjawan Wudtiwai, Anupong Makeudom, Suttichai Krisanaprakornkit, Peraphan Pothacharoen, Prachya Kongtawelert
Summary: The study demonstrates that hesperidin effectively reduces cell proliferation and migration of oral cancer cells by inhibiting PD-L1 expression and inactivating STAT1 and STAT3 signaling molecules. This suggests hesperidin could be a potential adjunctive treatment for oral cancer.
Article
Endocrinology & Metabolism
Mattabhorn Phimphilai, Peraphan Pothacharoen, Prachya Kongtawelert
Summary: This study revealed impaired osteogenic differentiation in approximately half of PBMC derived from type 2 diabetic patients receiving metformin monotherapy. Overexpression of both AGER and BAX/BCL2 was only demonstrated in PBMC isolated from diabetic patients with poor osteogenic differentiation. Age was shown to have a positive impact on RAGE overexpression, and both age and RAGE overexpression were identified as independent risk factors influencing osteogenic differentiation potential in PBMC isolated from T2DM.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Virology
Aitthiphon Chongchai, Sajee Waramit, Tunchanok Wongwichai, Jirawan Kampangtip, Thanyaluck Phitak, Prachya Kongtawelert, Amin Hajitou, Keittisak Suwan, Peraphan Pothacharoen
Summary: The study developed a targeted delivery strategy which successfully delivered transgenes to HACs using CAP.Phage particles, with the CAP ligand demonstrating preference for binding to pathogenic chondrocytes.
Article
Endocrinology & Metabolism
Mattabhorn Phimphilai, Peraphan Pothacharoen, Nipon Chattipakorn, Prachya Kongtawelert
Summary: This study found that prediabetic individuals still have preserved osteogenic differentiation ability in PBMC, and these cells show both suppression of cellular AGE receptors and apoptotic-related signals. Serum pentosidine is an independent risk factor for cellular AGE receptor expression and can be used as a good predictor for AGE receptor suppression in prediabetic individuals.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Plant Sciences
Peraphan Pothacharoen, Rujirek Chaiwongsa, Theerawut Chanmee, Orapin Insuan, Thanchanok Wongwichai, Phornpimon Janchai, Pilanee Vaithanomsat
Summary: Bromelain, a protease mixture found in pineapple, has beneficial biological properties and exhibits anti-arthritic activities. It protects cartilage from degradation and decreases the expression of inflammatory cytokines in synovial fibroblasts by suppressing NF-kappa B and MAPK signaling pathways.
Article
Cell Biology
Su Yati, Atiruj Silathapanasakul, Chakrarin Thakaeng, Mayuree Chanasakulniyom, Napat Songtawee, Sureerut Porntadavity, Peraphan Pothacharoen, Dumnoensun Pruksakorn, Prachya Kongtawelert, Pa-Thai Yenchitsomanus, Theerawut Chanmee
Summary: The release of extracellular vesicles (EVs) in response to chemotherapy can mediate the expression of programmed cell death ligand 1 (PD-L1) via the IL-1 signaling pathway in osteosarcoma cells. This study provides insights into the role of EVs in regulating PD-L1 expression and suggests the potential for combining IL-1 or PD-L1 targeting drugs with chemotherapy as a treatment option for osteosarcoma patients.
Article
Biology
Pattaralawan Sittiju, Parunya Chaiyawat, Dumnoensun Pruksakorn, Jeerawan Klangjorhor, Weerinrada Wongrin, Phichayut Phinyo, Rawikant Kamolphiwong, Areerak Phanphaisarn, Pimpisa Teeyakasem, Prachya Kongtawelert, Peraphan Pothacharoen
Summary: Novel or OS-specific biomarkers from GEO database show promise for developing diagnostic and tumor progression strategies. Three candidate genes, including COL5A2, ezrin, and vimentin, were identified as potential tools for OS detection and disease progression prediction. Liquid biopsy is an interesting non-invasive tool for measuring tumor material, with VIM and ezrin genes associated with EMT and metastasis in OS.
Article
Multidisciplinary Sciences
Prachya Kongtawelert, Chayanut Kaewmool, Thanyaluck Phitak, Mattabhorn Phimphilai, Peraphan Pothacharoen, Thuzar Hla Shwe
Summary: This study revealed that sesamin can inhibit high glucose-induced microglial activation and inflammatory responses, thereby reducing neurotoxicity. This suggests that sesamin may be a potential therapeutic compound for preventing diabetic-induced neurodegenerative diseases.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Orapin Insuan, Phornphimon Janchai, Benchaluk Thongchuai, Rujirek Chaiwongsa, Supaporn Khamchun, Somphot Saoin, Wimonrut Insuan, Peraphan Pothacharoen, Waraporn Apiwatanapiwat, Antika Boondaeng, Pilanee Vaithanomsat
Summary: The study found that bromelain can inhibit LPS-induced inflammatory responses, reduce the production of inflammatory cytokines, and achieve anti-inflammatory effects by regulating the expression of iNOS and COX-2, with purified rhizome bromelain showing stronger inhibitory effects than crude bromelain in RAW264.7 cells.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2021)
