Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated, homology-independent knock-in system

Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein–coupled receptors

(2017) Tomoaki Hirano et al.   MOLECULAR BIOLOGY OF THE CELL

Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46–IFT56 as well as on its nuclear localization signal

(2017) Teruki Funabashi et al.   MOLECULAR BIOLOGY OF THE CELL

The intraflagellar transport machinery in ciliary signaling

(2016) André Mourão et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

The democratization of gene editing: Insights from site-specific cleavage and double-strand break repair

(2016) Maria Jasin et al.   DNA REPAIR

Intraflagellar transport proteins 172, 80, 57, 54, 38, and 20 form a stable tubulin‐binding IFT‐B2 complex

(2016) Michael Taschner et al.   EMBO JOURNAL

Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex

(2016) Yohei Katoh et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Regulation of ciliary retrograde protein trafficking by the Joubert syndrome proteins ARL13B and INPP5E

(2016) Shohei Nozaki et al.   JOURNAL OF CELL SCIENCE

The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane

(2016) Yoshiki Tanaka et al.   MOLECULAR BIOLOGY OF THE CELL

In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

(2016) Keiichiro Suzuki et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair

(2016) Xiangjun He et al.   NUCLEIC ACIDS RESEARCH

In vivoblunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining

(2016) Jonathan M. Geisinger et al.   NUCLEIC ACIDS RESEARCH

DNA double-strand-break repair in higher eukaryotes and its role in genomic instability and cancer: Cell cycle and proliferation-dependent regulation

(2016) Emil Mladenov et al.   SEMINARS IN CANCER BIOLOGY

The Intraflagellar Transport Machinery

(2016) Michael Taschner et al.   Cold Spring Harbor Perspectives in Biology

CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing

(2016) Jonas Dehairs et al.   Scientific Reports

A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis

(2015) Ken Motohashi   BMC BIOTECHNOLOGY

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems

(2015) Tetsushi Sakuma et al.   Nature Protocols

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

A generic strategy for CRISPR-Cas9-mediated gene tagging

(2015) Daniel H. Lackner et al.   Nature Communications

The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation

(2015) Tatsuo Miyamoto et al.   Cell Reports

CRISPR/Cas9 Genome Editing in Caenorhabditis elegans : Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair

(2015) Iskra Katic et al.   G3-Genes Genomes Genetics

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

CRISPR/Cas9 and TALEN-mediated knock-in approaches in zebrafish

(2014) Thomas O. Auer et al.   METHODS

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Ciliopathies: The Trafficking Connection

(2014) Kayalvizhi Madhivanan et al.   TRAFFIC

Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering

(2014) Yukiko Kimura et al.   Scientific Reports

Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays

(2013) Satoshi Ota et al.   GENES TO CELLS

Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair

(2013) T. O. Auer et al.   GENOME RESEARCH

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA

(2013) Daisuke Mashiko et al.   Scientific Reports

The Genomic and Transcriptomic Landscape of a HeLa Cell Line

(2013) Jonathan J. M. Landry et al.   G3-Genes Genomes Genetics

In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration

(2012) Sandra Cristea et al.   BIOTECHNOLOGY AND BIOENGINEERING

Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joining

(2012) M. Maresca et al.   GENOME RESEARCH

Deletion of IFT20 in the mouse kidney causes misorientation of the mitotic spindle and cystic kidney disease

(2008) Julie A. Jonassen et al.   JOURNAL OF CELL BIOLOGY