Article
Oncology
Jacqueline M. Grible, Patricija Zot, Amy L. Olex, Shannon E. Hedrick, J. Chuck Harrell, Alicia E. Woock, Michael O. Idowu, Charles V. Clevenger
Summary: Through experiments on human breast epithelial cells, it was found that co-expression of hPRLrI and hPRLrL can lead to transformation, with higher levels of hPRLrI being associated with triple-negative breast cancer. The study suggests that hPRLrI may play a role in mammary transformation and represents a novel oncogenic mechanism.
Article
Multidisciplinary Sciences
Wen Bu, Yi Li
Summary: Mouse mammary glands are composed of ductal trees lined with epithelial cells, which are responsible for mammary gland function and tumorigenesis. Intraductal injection of a viral vector carrying genes of interest into the mouse mammary ductal tree is a critical step for evaluating gene function and generating tumor models. Lentiviral, retroviral, adenoviral, or AAV vectors can be used for this purpose. This study demonstrates the delivery of a gene of interest into mammary epithelial cells using intraductal injection of a viral vector, showing stable expression and oncogene-induced lesions and tumors.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Multidisciplinary Sciences
Wen Bu, Yi Li
Summary: Mouse mammary glands have ductal trees lined by epithelial cells with one opening at each nipple. Introducing genes into these epithelial cells is crucial for studying gene function and generating mammary tumor models. This can be done by intraductal injection of a viral vector carrying the genes. Different types of viral vectors can be used, such as lentiviruses and retroviruses. This study demonstrates the delivery of genes of interest into mammary epithelial cells using intraductal injection of a viral vector, and the subsequent expression and effects of these genes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Oncology
Yuying Fan, Xiaoli Ren, Yingxue Wang, Enshuang Xu, Shuang Wang, Ruidong Ge, Yun Liu
Summary: Metformin was found to induce apoptosis and cell cycle arrest in canine mammary gland tumor cells via the AMPK/AKT/mTOR signaling pathway, suggesting its potential therapeutic effect on CMGTs.
Article
Biochemistry & Molecular Biology
Limin Lang, Jisong Zheng, Shuyi Liang, Fenglin Zhang, Yiming Fu, Kaixin Deng, Fan Li, Xiaohua Yang, Junfeng Wang, Yuexiang Luo, Shilei Zhang, Xiaotong Zhu, Lina Wang, Ping Gao, Canjun Zhu, Gang Shu, Qianyun Xi, Yongliang Zhang, Qingyan Jiang, Songbo Wang
Summary: The browning of mammary fat suppresses the development of pubertal mammary gland, which is associated with elevated serum DOPC and inhibition of the PI3K/Akt pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Siwen Li, Jiaqi Xie, Yiang Bai, Zhihao Jiang, Keman Li, Chunling Wu
Summary: In this study, exposure to two representative synthetic phenolic antioxidants (BHA and BHT) at environmentally relevant levels was found to cause liver damage in grass carp by regulating the ROS/PI3K-mediated autophagic hyperactivation pathway, leading to hepatocyte death. These findings provide new insights into the potential mechanisms underlying liver damage caused by BHA and BHT in aquatic organisms and offer a new theoretical basis for ecological risk assessment of synthetic phenolic antioxidants.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Food Science & Technology
Garam An, Junho Park, Whasun Lim, Gwonhwa Song
Summary: This study aimed to investigate the harmful effects of folpet on bovine mammary glands and milk production using MAC-T cells. The results showed that folpet exhibited cytotoxicity against MAC-T cells, causing apoptosis and cell death. Furthermore, folpet treatment induced oxidative stress and activated MAPK signaling pathways. This is the first report highlighting the detrimental impacts of folpet on bovine mammary glands and the dairy industry, providing insights into the intracellular mechanisms using MAC-T cells.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Integrative & Complementary Medicine
Yue Liu, Fan Zhang, Xiaomeng Han, Ningyang Xu, Yu Zhao, Qige Wang, Jianan Wang, Bingjiu Lu, Yan Zhang
Summary: This study found that JPQT induces anti-atherosclerosis effects through anti-inflammatory and inhibiting JAK/STAT signaling pathways.
JOURNAL OF TRADITIONAL CHINESE MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Wen Li, Xiaoyu Long, Feng Li, Yu Cao, Juxiong Liu, Shoupeng Fu, Wenjin Guo, Guiqiu Hu
Summary: Lysine promotes the development of mammary glands in pubertal mice by enhancing the proliferation of mammary epithelial cells and activating the PI3K/AKT/mTOR signaling pathway.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2022)
Article
Agriculture, Dairy & Animal Science
Yusong Ge, Feng Li, Yuan He, Yu Cao, Wenjin Guo, Guiqiu Hu, Juxiong Liu, Shoupeng Fu
Summary: L-arginine stimulates the development of mammary glands in pubertal mice through the GPRC6A/PI3K/AKT/mTOR signaling pathway.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2022)
Article
Oncology
Phyllis van der Ploeg, Aniek Uittenboogaard, Anna M. J. Thijs, Hans M. Westgeest, Ingrid A. Boere, Sandrina Lambrechts, Anja van de Stolpe, Ruud L. M. Bekkers, Jurgen M. J. Piek
Summary: The clinical benefit of monotherapy with PI3K/AKT/mTOR inhibitors in patients with advanced or recurrent ovarian cancer is limited to a small subgroup, and selecting patients based on current biomarkers did not significantly improve the response rate. Due to the substantial toxicity associated with this treatment, it is recommended that PI3K/AKT/mTOR inhibitors should be used cautiously and preferably in the context of clinical trials. Improved biomarkers are needed to better select patients for this treatment.
GYNECOLOGIC ONCOLOGY
(2021)
Article
Cell Biology
Yu Cao, Yan Zhang, Lijun Ma, Jiaxin Wang, Wenjin Guo, Ji Cheng, Guiqiu Hu, Shoupeng Fu, Juxiong Liu
Summary: This study aimed to investigate the effects of niacin on mammary gland development in pubertal mice, and found that niacin stimulates mammary gland development through the Akt/mTOR and ERK1/2 signaling pathways by promoting the synthesis of cell proliferation markers.
CELL AND TISSUE RESEARCH
(2021)
Article
Surgery
Gaowu Hu, Wenquan Chen, Wei Peng, Zhen Huang, Zhanlin Dong, Yongqing Cao
Summary: This study found that cincumol can inhibit the malignant phenotypes of CRC cells by inhibiting the PI3K/AKT signaling pathway, suggesting that cincumol might be a potential anti-CRC agent.
ACTA CIRURGICA BRASILEIRA
(2022)
Article
Veterinary Sciences
Min-Jae Yoo, Jawun Choi, Ye-ji Jang, Sang-Youel Park, Jae-Won Seol
Summary: Our study demonstrates that palmatine exerts anti-cancer effects against CMTs by inhibiting the PI3K/AKT signaling pathway. It inhibits tumor growth, metastasis, and tube formation in vitro and in vivo, making it a potential canine-specific PI3K inhibitor for the treatment of CMTs.
BMC VETERINARY RESEARCH
(2023)
Article
Dermatology
Adina Eichner, Johannes Wohlrab
Summary: The use of Janus kinase inhibitors for chronic inflammatory diseases is increasingly recognized, but its pharmacodynamics greatly depend on dose regimen, treated indication, comedication, and comorbidity. Therefore, it is crucial to use them in accordance with approval.
JOURNAL DER DEUTSCHEN DERMATOLOGISCHEN GESELLSCHAFT
(2022)
Article
Multidisciplinary Sciences
Kazuhito Sakamoto, Patrick D. Radler, Barbara L. Wehde, Aleata A. Triplett, Hridaya Shrestha, Rosa-Maria Ferraiuolo, Foued Amari, Vincenzo Coppola, Apostolos Klinakis, Argiris Efstratiadis, Kay-Uwe Wagner
SCIENTIFIC REPORTS
(2020)
Review
Oncology
Rosa-Maria Ferraiuolo, Karoline C. Manthey, Marissa J. Stanton, Aleata A. Triplett, Kay-Uwe Wagner
Article
Audiology & Speech-Language Pathology
Shikha Tarang, Umesh Pyakurel, Michael D. Weston, Sarath Vijayakumar, Timothy Jones, Kay-Uwe Wagner, Sonia M. Rocha-Sanchez
Review
Oncology
Rayane Dennaoui, Hridaya Shrestha, Kay-Uwe Wagner
Summary: Pancreatic cancer research has made significant progress in understanding the molecular and developmental processes involved in the genesis of this highly malignant tumor type. Various models, including chemical carcingen-induced and genetically engineered animal models, are being developed and analyzed to study the biological significance of new molecular targets and mechanisms contributing to pancreatic cancer onset and progression.
CANCER AND METASTASIS REVIEWS
(2021)
Article
Oncology
Gabriel B. Mpilla, Md Hafiz Uddin, Mohammed N. Al-Hallak, Amro Aboukameel, Yiwei Li, Steve H. Kim, Rafic Beydoun, Gregory Dyson, Erkan Baloglu, William T. Senapedis, Yosef Landesman, Kay-Uwe Wagner, Nerissa T. Viola, Bassel F. El-Rayes, Philip A. Philip, Ramzi M. Mohammad, Asfar S. Azmi
Summary: The study highlights the need for more effective targeted approaches to sensitize PNETs to everolimus for better treatment outcomes. The dual inhibitor KPT-9274 targeting PAK4-NAMPT, when combined with everolimus, showed promising results in inhibiting PNET growth and formation.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Endocrinology & Metabolism
Hyunji Byun, Sojung Kwon, Kay-Uwe Wagner, Hyejin Shin, Hyunjung Jade Lim
Summary: Deficiency of Tsg101 in uterine epithelium causes implantation failure and may be due to epithelial defects. The study found that UECs harbor a necroptotic machinery that responds to death-inducing signals.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Patrick D. Radler, Barbara L. Wehde, Aleata A. Triplett, Hridaya Shrestha, Jonathan H. Shepherd, Adam D. Pfefferle, Hallgeir Rui, Robert D. Cardiff, Charles M. Perou, Kay-Uwe Wagner
Summary: Claudin-low breast cancer is an aggressive subtype mainly composed of triple-negative mammary tumor cells with stem cell-like and mesenchymal features. The consistent activation of oncogenic RAS signaling and regulators of EMT play crucial roles in maintaining the cellular plasticity and characteristics of claudin-low mammary cancer cells.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yunguang Sun, Ning Yang, Fransiscus E. Utama, Sameer S. Udhane, Junling Zhang, Amy R. Peck, Alicia Yanac, Katherine Duffey, John F. Langenheim, Vindhya Udhane, Guanjun Xia, Jess F. Peterson, Julie M. Jorns, Marja T. Nevalainen, Romain Rouet, Peter Schofield, Daniel Christ, Christopher J. Ormandy, Anne L. Rosenberg, Inna Chervoneva, Shirng-Wern Tsaih, Michael J. Flister, Serge Y. Fuchs, Kay-Uwe Wagner, Hallgeir Rui
Summary: A humanized prolactin mouse model was developed to establish therapy-naive ER+ breast cancer tumors and study treatment resistance mechanisms, revealing new insights into precision medicine approaches for this type of cancer.
Review
Oncology
Md. Hafiz Uddin, Mohammed Najeeb Al-Hallak, Philip A. Philip, Ramzi M. Mohammad, Nerissa Viola, Kay-Uwe Wagner, Asfar S. Azmi
Summary: Pancreatic cancer is the fourth leading cause of cancer death in the United States, with PDAC accounting for over 90% of cases and a low survival rate. MiRNAs have been identified as sensitive biomarkers in pancreatic cancer and are found in stable forms within exosomes. Further research on exosomal miRNAs is necessary for improved diagnostic, prognostic, and therapeutic strategies.
Article
Medicine, Research & Experimental
Cancan Lyu, Yuanchao Ye, Maddison M. Lensing, Kay-Uwe Wagner, Ronald J. Weigel, Songhai Chen
Summary: In HER2(+) breast cancer, overactivation of HER2 leads to aberrant G(i/o)-GPCR signaling, promoting cancer progression and resistance to HER2-targeted therapy. Pharmacologically deactivating GPCR signaling can block tumor growth and enhance therapeutic efficacy.
Article
Multidisciplinary Sciences
Patrick D. Radler, Kerry Vistisen, Aleata A. Triplett, Rayane Dennaoui, Yong Li, Hridaya Shrestha, Rosa-Maria Ferraiuolo, Amalraj Thangasamy, Dieter Saur, Kay-Uwe Wagner
Summary: The researchers developed a transgenic mouse line expressing an optimized Flp recombinase, demonstrating the versatile applicability of the new MMTV-Flp strain in manipulating genes in mammary gland cells. By combining two recombinases, genes can be deleted or activated in established tumors, showing the feasibility of gene manipulation in neoplastic mammary epithelial cells.
SCIENTIFIC REPORTS
(2021)
Review
Oncology
Madison N. Wicker, Kay-Uwe Wagner
Summary: Cellular plasticity, the ability of cells to change their identity, plays a crucial role in mammary gland development and breast cancer progression. This review provides a comprehensive overview of the factors and mechanisms that promote cellular plasticity in the mammary gland. It discusses changes in cell identity during normal development, the role of the gestation cycle, and highlights the importance of the microenvironment and extracellular matrix. The review also explores cellular reprogramming during mammary tumorigenesis, focusing on the origin of basal-like breast cancers and the role of oncogenic signaling networks. Additionally, recent advances in genetically engineered models to study cellular plasticity in vivo are discussed.
Article
Biochemistry & Molecular Biology
Dai Le, Soyeon Lim, Kwang Wook Min, Joon Woo Park, Youjoung Kim, Taejeong Ha, Kyeong Hwan Moon, Kay-Uwe Wagner, Jin Woo Kim
Summary: The distribution of membrane proteins in RPE varies during different developmental stages, indicating developmental regulation of protein trafficking. Deletion of Tsg101 disrupts RPE polarity, leading to irregular aggregates and non-polarized distribution of cell adhesion proteins and activation of epidermal growth factor receptor signaling, highlighting the importance of ESCRT-mediated protein trafficking for RPE cell polarity development and maintenance.
MOLECULES AND CELLS
(2021)
Article
Cell Biology
Karina A. Pena, Sofya Savransky, Breanna Lewis
Summary: Compartmentalization of GPCR signaling is an emerging topic that emphasizes the importance of spatial bias in signaling for physiological relevance. PTH1R was the first GPCR discovered to signal via cAMP from endosomes, challenging the conventional model of GPCR signaling. The location of cAMP generation determines the physiological outcomes of GPCR signaling.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Cheng-Xu Ma, Xiao-Ni Ma, Jin-Jin Liu, Cong-Hui Guan, Ying-Dong Li, Nan Zhao, Didac Mauricio, Song-Bo Fu
Summary: The downregulation of primary cilia (PCs) due to BRAFV600E mutation contributes to the aggressiveness and lymph node metastasis of Papillary Thyroid Cancer (PTC).
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Kejun Wu, Cuihua Huang, Wenrong Zheng, Yubin Wu, Qintao Huang, Menghua Lin, Ruonan Gao, Liqin Qi, Guanlian He, Xiaoying Liu, Xiaohong Liu, Linxi Wang, Zhou Chen, Libin Liu
Summary: Recurrent non-severe hypoglycemia in patients with diabetes is associated with cognitive impairment. This study found that this condition is associated with reduced mitophagy in the hippocampus, leading to mitochondrial dysfunction and neurological impairment.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Yifei Lv, Yizhou Huang, Huiyu Fan, Yunxiu Zhao, Linjuan Ma, Yibing Lan, Chunming Li, Peiqiong Chen, Zheng Lou, Jianhong Zhou
Summary: Before menopause, females have a lower incidence of cardiovascular disease than age-matched males, possibly due to the protective effects of sex hormones. 17 beta-E2 inhibits THBS1 expression, preventing cell senescence and apoptosis, and counteracts oxidative stress by suppressing the TGF-beta/Smad signaling pathway.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Ana C. L. Camargo, Flavia B. Constantino, Sergio A. A. Santos, Ketlin T. Colombelli, Luiz M. F. Portela, Matheus N. Fioretto, Luisa A. Barata, Guilherme T. Valente, Carlos S. Moreno, Luis A. Justulin
Summary: This study examined the effects of maternal malnutrition on the transcriptomic landscape of the ventral prostate in rats. It found that changes in molecular pathways related to cellular development and tissue morphogenesis were associated with maternal malnutrition. The Abcg1 gene was found to be deregulated in both malnourished rats and prostate cancer models and patients.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Sandra Romero-Cordoba, Mayel Chirinos, Nancy Noyola-Martinez, Nayeli Torres-Ramirez, Mitzi Garcia-Olivares, Juan Pablo Aragon-Hernandez, Ixchel Ramirez-Camacho, Rosa Zuniga, Fernando Larrea, Ali Halhali, David Barrera
Summary: This study analyzed the effects of calcitriol, TGF-131, and their combination on human trophoblast cells. The results showed that the combination treatment modified the transcriptional landscape and mainly affected the storage, activity, and metabolism of lipids, which may have an impact on placental development.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Traver J. Wright, Richard B. Pyles, Melinda Sheffield-Moore, Rachel R. Deer, Kathleen M. Randolph, Kristen A. Mcgovern, Christopher P. Danesi, Charles R. Gilkison, Weston W. Ward, Jayson A. Vargas, Peyton A. Armstrong, Sarah E. Lindsay, Mohammed F. Zaidan, Justin Seashore, Tamara L. Wexler, Brent E. Masel, Randall J. Urban
Summary: This study investigates the persistent neurologic symptoms in COVID-19 patients after recovery and explores the association between these symptoms and disrupted growth hormone secretion and gastrointestinal discomfort.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Li-Li Chen, Ya-Qin Li, Zhi-Hui Kang, Xuan Zhang, Su-Yan Gu, Na Wang, Xue-Yan Shen
Summary: Defects in trophoblastic EMT caused by dysregulation of circTNRC18's interaction with LIN28A play a vital role in the development of preeclampsia. LIN28A overexpression suppresses circTNRC18-mediated inhibition of trophoblast migration, invasion, and EMT, while LIN28A knockdown promotes them. Furthermore, circTNRC18 regulates the intracellular distribution of LIN28A and the expression of insulin-like growth factor II, affecting cell migration and invasion. Targeting the circTNRC18-LIN28A regulatory axis may provide a novel treatment approach for preeclampsia.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)