Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 443, Issue C, Pages 146-154Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2017.01.022
Keywords
Vasoactive intestinal polypeptide; Progesterone; Trophoblast-derived cells; Phagocytosis; Maternal placental interface
Categories
Funding
- National Agency of Sciences and Technology ANPCyT [PICT 2013-1632, 2014-0657]
- University of Buenos Aires [UBACyT 20020130100040BA, UBACyT 20020090200034]
Ask authors/readers for more resources
Trophoblast cells produce several inmmuneregulators like the Vasoactive Intestinal Peptide (VIP) and P4 targeting multiple circuits, and also display an intese phagocytic ability allowing embryo implantation in a tolerogenic context. Here, we explored whether P4 and VIP- crosstalk modulates trophoblast cell function, focus on the phagocytic ability and the immune homeostasis maintenance. P4 enhanced the phagocytosis in trophoblast-derived cells quantified by the engulfment of latex-beads or eryptotic erythrocytes. P4 and VIP modulated the balance of anti/pro-inflammatory mediators, increasing TGF-beta expression, with no changes in IL-1, IL-6, or nitrites production. This modulation was accompained by transcription factor expression changes that could turn on tolerogenic programs represented by increased PPAR-gamma and decreased IRF-5 expression. Finally, P4 stimulated VPAC2 expression in trophoblast cells and VPAC2 over-expression enhanced phagocytosis mimicking P4-effect. Therefore, P4 and VIP network enhances the phagocytic ability of trophoblast-derived cells, through a mechanism involving VPAC2 accompained with an anti-inflammatory context. (C) 2017 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available