Article
Mechanics
Xiao Hu, Jianzhong Lin, Yu Guo, Xiaoke Ku
Summary: The inertial focusing of elliptical particles and the formation of self-organizing trains in a channel flow are studied using the lattice Boltzmann method. Different particle characteristics such as aspect ratio, concentration, Reynolds number, and blockage ratio affect the formation and stability of the particle trains.
Article
Chemistry, Physical
Kuntal Patel, Holger Stark
Summary: This study investigates the hydrodynamic interaction of two particles in inertial microfluidics, revealing the dynamics of different mono- and bi-dispersed particle pairs with varying starting positions, deformability, and shapes. The results show that stable pairs become unstable when increasing particle stiffness, and pairs with capsules in the same channel half are more likely to become unstable than pairs with capsules in opposite channel halves.
Article
Mechanics
M. Lopez, M. G. Cabezas, J. M. Montanero, M. A. Herrada
Summary: In this study, the stability of hydrodynamic focusing to produce microemulsions is investigated experimentally and numerically. It is found that the distance between the feeding capillary and the focusing orifice affects the liquid ejection stability. Confined selective withdrawal enhances the stability of the microdripping mode. Surfactants stabilize the meniscus and promote the transition from microdripping to jetting. The global stability analysis accurately predicts the critical flow rate ratio and the droplet emission frequency.
JOURNAL OF FLUID MECHANICS
(2022)
Article
Computer Science, Interdisciplinary Applications
Samuel Christensen, Raymond Chu, Christopher Anderson, Marcus Roper
Summary: In this study, we propose a numerical method that calculates the migration velocities of particles in inertial microfluidic channels of arbitrary cross section by representing particles using singularities. We provide refinements to asymptotic analysis to improve the regularity of the singularity approximation, making finite element approximations of flow and pressure fields more effective. Sample results demonstrate that the method is computationally efficient and capable of capturing bifurcations in particle focusing positions resulting from changes in channel shape and Reynolds number.
JOURNAL OF COMPUTATIONAL PHYSICS
(2022)
Article
Biochemical Research Methods
Pablo Rodriguez-Mateos, Bongkot Ngamsom, Charlotte E. Dyer, Alexander Iles, Nicole Pamme
Summary: The early detection of pathogenic microorganisms is crucial for diagnosing and preventing health and safety emergencies. Traditional detection methods rely on time-consuming culturing procedures and biochemical assays, taking over 24 hours for a diagnosis. The main challenge lies in the low concentration of pathogens within complex samples. Inertial microfluidic manipulation in curved glass channels shows promise for automating and parallelizing particle and cell separation.
Article
Chemistry, Physical
Sanaz Sabaghi, Pedram Fatehi
Summary: In this study, cationic kraft lignin (CKL) polymers with different degrees of grafting were synthesized to investigate the impact of chain extension on the physicochemical behavior of CKL in an aqueous solution. The results showed that the architecture of cationic monomers significantly influenced the structure, rheological properties, and adsorption performance of CKLs on a solid surface, with more observable effects at higher grafting ratios.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Pharmacology & Pharmacy
Roni Sverdlov Arzi, Asaf Kay, Yulia Raychman, Alejandro Sosnik
Summary: Nanoprecipitation and microfluidics technology are effective methods for producing pure drug nanoparticles and optimizing product performance. The use of specific microfluidic devices can lead to the production of rounded, optimized nanoparticles.
Article
Engineering, Electrical & Electronic
Shuang Chen, Zongqian Shi, Jiajia Sun, Shenli Jia, Mingjie Zhong, Yuxin Ma
Summary: Inertial microfluidic is a widely applied technology that uses inertial lift force and Dean Vortices to manipulate particles or bio-samples. This study introduces a split-recombination microchannel for high flow rate particle focusing and separation, utilizing the combined effects of inertial lift force, curvature-induced Dean drag force, and the structure of split and recombination. The designed microchannel not only enhances particle focusing but also enables the separation of different-sized particles with high throughput.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2022)
Article
Nanoscience & Nanotechnology
Xiaofei Yuan, Andrew Glidle, Hitoshi Furusho, Huabing Yin
Summary: Traditional two-dimensional fluid control strategies in microfluidic cell sorting have limitations in terms of precision and purity, while devices based on three-dimensional hydrodynamics offer better flow-focusing characteristics but are restricted by arbitrary sample positioning. The 3D hydrodynamic focusing sorting platform designed in this study has advantages in precisely controlling sample velocity and position, showing potential for high-accuracy Raman activated sorting.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Engineering, Electrical & Electronic
Yogesh M. Patel, Ritika Malik, Kedar Khare, Supreet Singh Bahga
Summary: We propose a microfluidic holographic cytometry technique that uses three-dimensional hydrodynamic focusing to accurately visualize, classify, and quantify cells and particles in a mixture. This technique achieves high-resolution holographic imaging without the need for computationally-expensive numerical refocusing used in existing methods. It also prevents cell clustering and can be fabricated at a low cost. The technique shows promise for label-free classification and quantification of infected cells in various applications.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2023)
Article
Chemistry, Analytical
Lei Zhao, Mengqi Gao, Yanbing Niu, Jianchun Wang, Shaofei Shen
Summary: A novel microchannel structure was developed for flow-rate and particle-size insensitive inertial focusing; efficient focusing of particles and tumor cells at different flow rates was achieved through enhanced secondary flow; providing important reference for the development of portable inertial microfluidic devices.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Jeong-ah Kim, Yo-han Choi, Wonhee Lee
Summary: Inertial focusing allows passive manipulation of cells or microparticles with high throughput. We designed a flexible, thin PDMS microfluidic device to control inertial focusing positions using a 3D-curved triangular channel. The Dean flows induced by the 3D curvature alter the focusing positions, which can be controlled by adjusting the structural parameters.
Article
Nanoscience & Nanotechnology
O. Mesdjian, N. Ruyssen, M-C Jullien, R. Allena, J. Fattaccioli
Summary: Small microfluidic traps were designed using single-layer direct laser writing into photoresist, showing that the orientation of the main flow relative to the traps affects trapping efficiency and homogeneity. The study found that diagonal flows result in a more uniform distribution of trap loading compared to straight flows.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Chemistry, Physical
Vidhi Jain, Vashishtha B. Patel, Beena Singh, Dharmesh Varade
Summary: Microfluidics is a promising field with wide applications, providing fine control over particle size and speed in micro- and nanoscale self-assembled systems. It is a powerful tool for controlling physical and chemical properties of fluids and governing self-assembly processes, with potential impacts in various industries.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Analytical
Zhenna Chen, Beibei Chen, Man He, Bin Hu
Summary: A negative magnetophoresis focusing microchip was developed and coupled to ICP-MS for single-cell analysis, achieving high sample throughput. Study on uptake behaviors revealed that MCF-7 cells exhibited more remarkable heterogeneity when treated with ZnO NPs, with lower uptake content compared to Zn2+.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Dongtao Zhou, Yi Yin, Zhenxing Zhu, Yanfeng Gao, Jingjing Yang, Yongchun Pan, Yujun Song
Summary: Inflammatory bowel disease (IBD) is a chronic autoimmune disease with increasing incidence globally. This study presents an orally administered nanosensor that releases ultrasmall platinum nanoclusters (PtNCs) in IBD-related inflammatory environments, enabling non-invasive monitoring and diagnosis.
Article
Chemistry, Multidisciplinary
Xiaowei Luan, Yongchun Pan, Yuyu Zhou, Dongtao Zhou, Wenjian Zhao, Fei Zeng, Zhenxing Zhu, Qiangbing Lu, Qianglan Lu, Yanfeng Gao, Guanzhong He, Minghui Lu, Yujun Song
Summary: This study developed a pH-responsive reversible self-assembled Cu2-xSe-BSA nanodrug that can be renal cleared and selectively targeted aggregated in cancer lysosomes, enhancing tumor accumulation and retention. Furthermore, the nanodrug also demonstrated enhanced photoacoustic imaging signal, photothermal therapy, and chemodynamic therapy capability.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Chemistry, Medicinal
Yanping Wang, Yanfeng Gao, Yujun Song
Summary: Urine biopsy, as an emerging liquid biopsy strategy, offers advantages such as non-invasiveness, large sample volume, and simple sampling operation. Microfluidics, with its miniaturization, low cost, high integration, high throughput, and low sample consumption, has shown outstanding performance in urine biopsy for cancer detection.
Article
Chemistry, Multidisciplinary
Yayao Li, Yongchun Pan, Chao Chen, Zekun Li, Shiyu Du, Xiaowei Luan, Yanfeng Gao, Xin Han, Yujun Song
Summary: In this study, a pH/glutathione (GSH) dual stimuli-responsive CRISPR/Cas9 gene-editing nanoplatform combined with calcium-enhanced CO gas therapy was established for precise anticancer therapy. The platform demonstrated the ability to regulate the tumor microenvironment, perform gene ablation, and interfere with the ROS signaling pathway, showing great potential for future clinical applications in precise nanomedicine.
Review
Chemistry, Analytical
Stephane Colin, Jose M. Fernandez, Christine Barrot, Lucien Baldas, Slaven Bajic, Marcos Rojas-Cardenas
Summary: Thermometry techniques have been widely developed for analyzing thermal properties of fluid flows, but their application in analyzing gas flows at the microscale is not yet well developed. This review analyzes the current thermometry techniques adapted to microflows, with a focus on optical techniques and the challenges they face. Special attention is given to the potential and non-intrusiveness of Raman and molecular tagging thermometry techniques.
Article
Nanoscience & Nanotechnology
Yanfeng Gao, Dongtao Zhou, Qin Xu, Jingjing Li, Wen Luo, Jingjing Yang, Yongchun Pan, Ting Huang, Yanping Wang, Bangshun He, Yujun Song, Yuzhen Wang
Summary: In this study, a fluorescent bacteria counting immunoassay (FBCIA) strategy was developed for ultrasensitive quantification of protein biomarkers. The strategy involved a cascade signal conversion and amplification strategy, which enabled the conversion of target protein concentration to countable bacterial number. The developed platform demonstrated highly sensitive detection of CEA and PSA with low detection limits, and a smartphone device was also developed for point-of-care counting. The good consistency between FBCIA and clinical ELISA validated the high reliability and promising potential of the platform in clinical applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Yanfeng Gao, Yanping Wang, Xinli Liu, Zhenxing Zhu, Zhun Li, Zhibin Zhang, Yi Yin, William Chi Shing Cho, Yujun Song, Yuzhen Wang
Summary: We developed a signal amplification method based on streamlined click reactions to immobilize large amounts of hemin on graphene oxide nanosheets, allowing for highly sensitive detection of various biological targets. The designed platform shows great promise for graphene-based nanozymes and sensitive biosensing in a wider range of applications.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Yanfeng Gao, Yanping Wang, Yuzhen Wang, Pascale Magaud, Yuta Liu, Fei Zeng, Jingjing Yang, Lucien Baldas, Yujun Song
Summary: Accurate, sensitive, and highly selective detection of biological targets is crucial in various fields. The combination of nanocatalysis and microfluidics has shown great success in bioanalysis and offers numerous applications for different biological targets. This review summarizes the recent developments in nanocatalysis-assisted bioanalytical microfluidic devices, including various catalytically active nanomaterials, microfluidic approaches, detection techniques, and their applications. The integration of nanocatalysts for signal conversion and amplification is emphasized to enhance detection sensitivity. Furthermore, the challenges and future trends in nanocatalysis-assisted microfluidics are discussed, aiming to guide the development of practical point-of-care testing devices.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yongchun Pan, Xiaowei Luan, Yanfeng Gao, Fei Zeng, Xuyuan Wang, Dongtao Zhou, Wanqi Li, Yuzhen Wang, Bangshun He, Yujun Song
Summary: Researchers have designed a cancer-activated nanomachine based on porous upconversion nanoparticles for precise phototherapy. The nanosystem contains a telomerase substrate primer and encapsulates 5-aminolevulinic acid and D-arginine. After coating with hyaluronic acid, the nanomachine can easily enter tumor cells and induce efficient accumulation of protoporphyrin IX via biosynthetic pathway. The nanomachine can respond to near-infrared light and generate active singlet oxygen through Förster resonance energy transfer. Moreover, it can relieve tumor hypoxia by converting D-arginine into nitric oxide, thereby improving the phototherapy effect.
Article
Chemistry, Multidisciplinary
Yanfeng Gao, Yanping Wang, Bangshun He, Yongchun Pan, Dongtao Zhou, Mengqiu Xiong, Yujun Song
Summary: Colonization of cancer cells at secondary sites relies on the formation of metastatic microenvironments regulated by single-cell metabolism traits. A microfluidic platform has been developed for high-throughput monitoring of tumor cell metabolites, allowing evaluation of tumor malignancy and screening of anti-metastatic drugs. The platform shows potential for clinical application by efficiently detecting aggressive cancer cells in unprocessed blood samples.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Materials Science, Multidisciplinary
Dingdong Zhang, Guillermo Lopez-Quesada, Samuel Bergdolt, Stefan Hengsbach, Klaus Bade, Stephane Colin, Marcos Rojas-Cardenas
Summary: The two-photon-polymerization (TPP) fabrication technique was used to create three-dimensional micro-structures for rarefied gas flows. The geometry of the micro-tubes was characterized using various measurement techniques. Experimental measurements of nitrogen gas flow rates were compared to theoretical values, and the results showed good agreement. This study demonstrates the feasibility of using TPP fabrication technique to create well controlled 3D microsystems for rarefied gas flows applications.
Review
Pharmacology & Pharmacy
Yanping Wang, Yanfeng Gao, Yongchun Pan, Dongtao Zhou, Yuta Liu, Yi Yin, Jingjing Yang, Yuzhen Wang, Yujun Song
Summary: New drug discovery is facing increasing pressure from various domains, particularly the pharmaceutical industry and healthcare services. Preclinical assessment of drug efficacy and safety is crucial for reducing costs and time in drug development. Organ-on-a-chip, an in vitro model that mimics human organ functions, provides a potential alternative to animal models for efficient preclinical screening of drug candidates. This review provides insights into the design, advances, challenges, and future prospects of organ-on-a-chip in drug screening, highlighting its potential for drug development, therapeutic innovation, and precision medicine.
ACTA PHARMACEUTICA SINICA B
(2023)
Review
Thermodynamics
Georges C. Saliba, Ahmad Batikh, Stephane Colin, Lucien Baldas
Summary: Impinging jets are widely used in various applications, such as cooling turbine blades and heat management of electronic systems, due to their effectiveness and adaptability. Researchers have studied variations on the classic setup of impinging jets, including introducing periodic disturbances, to improve heat transfer. The methods of producing periodic disturbances vary in complexity and implementation cost.
ASME JOURNAL OF HEAT AND MASS TRANSFER
(2023)
Article
Chemistry, Analytical
Yuanyue Han, Xinli Liu, Qiao Zhao, Yanfeng Gao, Dongtao Zhou, Wenxiu Long, Yuzhen Wang, Yujun Song
Summary: A simple aptazyme-induced cascade signal amplification (ACSA) was integrated with a triple-channel volumetric bar-chart chip (TV-Chip) for the visual quantification of aflatoxin B1 (AFB1) and adenosine triphosphate (ATP). Bifunctional aptazymes modified on magnetic silica nanoparticles (MSNPs) were used as sensing probes. The detection limits of AFB1 and ATP were easily achieved without additional instruments. The results were in good agreement with a commercial AFB1 ELISA kit, showing the accuracy and reliability of this method. The ACSA-based TV-Chip holds great promise for on-site and real-time quantitation of trace targets.