Journal
MICROCHIMICA ACTA
Volume 184, Issue 8, Pages 2541-2549Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-017-2257-5
Keywords
ZnO; DNA dendrimers; Photoelectrochemical detection; Biosensor; Signal amplification; Chemical bond; Cyclic voltammetry; Electrochemical impedance spectroscopy; Photocurrent; Early diagnose
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Funding
- National Natural Science Foundation of China [51602053, 21375017]
- Fujian Natural Science Foundation [2015 J05020]
- Natural Science Foundation for Distinguished Young Scholars of Fujian Province [2013 J06003]
- Program for New Century Excellent Talents of Colleges and Universities in Fujian Province [JA13130, JA13088]
- Youth Scientific Research Program of Fujian Provincial Health and Family Planning Commission [2014-1-39]
- Nursery Scientific Research Foundation of Fujian Medical University [2014MP008]
- Professor Foundation of Fujian Medical University [JS14009]
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The work describes a photoelectrochemical (PEC) biosensor for the detection of DNA. It employs oriented hierarchical ZnO flower-rod architectures (ZnO FRs) and DNA dendrimers. ZnO FRs act as photoactive material that yields a photocurrent of up to 23 mu A. The photogenerated electron transfer is inhibited once the probe DNA and the target DNA oligonucleotides hybridize, and this results in a reduced photocurrent. The use of DNA dendrimers with scores of DNA branches further amplifies the signal of the PEC biosensor. The PEC sensor displays a response that is linear in the DNA concentration range from 10 fM to 0.1 mu M with a detection limit of 3.7 fM ( at S/N = 3). The sensor was applied to the determination of DNA in human serum samples and was found to work with acceptable accuracy. Due to the use of ZnO FRs and DNA dendrimers, the assay is highly sensitive, rapid, and repeatability.
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