Article
Food Science & Technology
Sara Asgari, Rajiv Dhital, Azlin Mustapha, Mengshi Lin
Summary: In this study, a surface-enhanced Raman spectroscopy (SERS) optofluidic sensor was developed to simultaneously separate and detect Escherichia coli O157:H7 and Salmonella in lettuce and packed salad. The method showed high sensitivity and low detection limit, with a short analysis time. It can be considered as a promising rapid and efficient approach for pathogen screening in food samples.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Marketa Benesova, Silvie Bernatova, Filip Mika, Zuzana Pokorna, Jan Jezek, Martin Siler, Ota Samek, Filip Ruzicka, Katarina Rebrosova, Pavel Zemanek, Zdenek Pilat
Summary: Efficient separation and identification of pathogenic bacterial strains is crucial for modern society, with applications in medical diagnostics, drug discovery, biodefense, and food safety. A fast and reliable method for antibody-based immobilization of bacteria onto a gold-plated glass surface, followed by detection using strain-specific antibodies linked to SERS-tags, was developed. The procedure utilizes accessible and affordable materials, and shows potential for rapid identification of bacterial pathogens.
Article
Chemistry, Analytical
Nolan File, Joseph Carmicheal, Alexey V. Krasnoslobodtsev, Nicole C. Japp, Joshua J. Souchek, Sudesna Chakravarty, Michael A. Hollingsworth, Aaron A. Sasson, Gopalakrishnan Natarajan, Prakash G. Kshirsagar, Maneesh Jain, Chihiro Hayashi, Wade M. Junker, Sukhwinder Kaur, Surinder K. Batra
Summary: SERS immunoassay biosensors have great potential for clinical diagnostics due to their high sensitivity and the growing interest in multi-marker panels. However, difficulties in designing compatible extrinsic Raman labels have hindered their development. Previous studies have focused on selecting Raman reporter molecules (RRMs) based on their spectroscopic characteristics for multiplexing, as well-differentiated spectra are essential for simultaneous detection. However, these candidates often induce aggregation of SERS nanotags made of gold nanoparticles. Therefore, a better understanding of factors affecting the aggregation of RRM-coated gold nanoparticles is needed.
Article
Biophysics
Chongwen Wang, Chaoguang Wang, Jiaxuan Li, Zhijie Tu, Bing Gu, Shengqi Wang
Summary: Researchers have developed a bi-channel surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFA) technique using nanostickers for sensitive and multiplexed detection of bacteria. The results showed that the nanostickers could rapidly detect multiple pathogens with high sensitivity and multiplex analysis capability.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Sylwia M. Berus, Monika Adamczyk-Poplawska, Beata Mlynarczyk-Bonikowska, Evelin Witkowska, Tomasz Szymborski, Jacek Waluk, Agnieszka Kaminska
Summary: The method of comprehensive SERSchemometric analysis of five bacterial pathogens responsible for sexually transmitted diseases showed high accuracy in identifying individual species within the Neisseria genus. This approach allows for simple and non-invasive analysis of clinical samples, with fast analysis time, culture-free identification, and sensitive and reliable SERS-based diagnosis of STDs.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Enlai Yang, Dan Li, Pengkun Yin, Qiyue Xie, Yu Li, Qingyu Lin, Yixiang Duan
Summary: The study developed a novel SERS strategy based on a three-dimensional DNA walker for quantitative analysis of Salmonella typhimurium. The method achieved high sensitivity detection of bacteria.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biochemistry & Molecular Biology
Elisabetta Canetta
Summary: Raman scattering is widely used in cancer nanomedicine for its high spatial resolution, chemical specificity, and multiplexity. Recent advancements in Raman spectroscopy have led to the development of various applications in cancer diagnostics and therapy. Surface-enhanced Raman spectroscopy (SERS) is also utilized in cancer nanomedicine for enhanced Raman signals and applications in cancer diagnosis and immunotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Yingkai Chen, Songxin Yang, Xinrui Shi, Zhaoyuan He, Hao Peng, Guimin Gu, Xiaolu Pang, Hailan Chen, Yueliang Wang, Longhua Guo
Summary: A surface-enhanced Raman scattering lateral flow immunoassay sensor (SERS-LFIA) was developed for rapid, ultrasensitive, and quantitative detection of Salmonella enteritidis (S. enteritidis). This method allowed for quantitative detection of S. enteritidis in concentrations ranging from 1x103 to 1x107 CFU mL-1, with limits of detection of 1x105 CFU mL-1 and 1.36x102 CFU mL-1 based on naked eye observation and SERS signal analysis, respectively. The SERS-LFIA showed no cross-reaction with other serotypes of Salmonella or common pathogens, and had a coefficient of variation less than 10% for repetitive tests. The method also demonstrated good recovery rates when applied to spiked feces and eggshells. This SERS-LFIA method has the potential to be a promising tool for the early detection of S. enteritidis in various clinical samples.
MICROCHEMICAL JOURNAL
(2023)
Review
Chemistry, Analytical
Vahid Eskandari, Saeideh Mehmandoust, Zahra Farahani, Negin Poorbeik Mohammad, Amin Hadi
Summary: The special features of liposome-based drug delivery systems have proven useful in overcoming biological and pharmacodynamic challenges. Liposomes, especially those used for targeted drug delivery to pathological tissues, are considered the simplest mimic of biological cells in medicine, attracting the attention of many researchers. Surface-enhanced Raman scattering (SERS) offers nondestructive analysis and high detection sensitivity, making it ideal for single molecular detection. This study reviews recent literature on SERS-based biosensors for enhancing diagnostic and therapeutic platforms, drug delivery systems, and pharmaceutical applications, aiming to aid researchers in making more advanced and successful systems in this sensitive and important field.
VIBRATIONAL SPECTROSCOPY
(2023)
Article
Biochemical Research Methods
Jingyu Ding, Qingqing Lin, Jiameng Zhang, Glenn M. Young, Chun Jiang, Yaoguang Zhong, Jianhua Zhang
Summary: Salmonella is a prevalent pathogen worldwide, causing serious morbidity and mortality. The most common foodborne pathogenic serovars in the EU and China are reported to be Salmonella Enteritidis, Salmonella Typhimurium, and Salmonella Paratyphi. A new analytical method combining surface-enhanced Raman spectroscopy with multi-scale convolutional neural network was developed to efficiently detect and distinguish these serovars, achieving a recognition accuracy of over 97%. The findings suggest the feasibility of combining SERS spectroscopy with multi-scale CNN not only for Salmonella serotype identification, but also for other pathogen species and serovar identifications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Matthew Eady, Gayatri Setia, Bosoon Park, Bin Wang, Jaya Sundaram
Summary: This study investigates a Salmonella detection method using surface-enhanced Raman spectroscopy (SERS) to reduce the time required for confirmation. Chicken rinses containing Salmonella Typhimurium (ST) were analyzed using SERS and compared to traditional plating and PCR analyses. The results show that the SERS method, combined with a support vector machine (SVM) classification algorithm, can accurately differentiate between ST and non-Salmonella samples with a classification accuracy of 96.7%.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Chemistry, Physical
Aswathy Vasudevan, Vasyl Shvalya, Martin Kosicek, Janez Zavasnik, Andrea Jurov, Neelakandan M. Santhosh, Aleksander Zidansek, Uros Cvelbar
Summary: Cold-atmospheric plasma-assisted deposition is an efficient technique for synthesizing gold nanoparticles, and the presence of ethanol promotes the reduction of gold to nanostructures. Nanostructured thin films can be created within a short time by controlling plasma parameters. The deposited gold nanoparticles exhibit advanced plasmonic surface-enhanced Raman scattering responses, making them potential substrates for fast and reliable optical analytical bio-chemical sensing.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Review
Oncology
Alexandra Teixeira, Luis Carreira, Sara Abalde-Cela, Belem Sampaio-Marques, Anabela C. Areias, Paula Ludovico, Lorena Dieguez
Summary: Acute myeloid leukemia (AML) is a common type of leukemia in adults, with limited response to conventional therapies and high recurrence rate. The development of earlier and more accurate diagnostic tools is crucial for improving patient prognosis.
Article
Chemistry, Analytical
Shiliang Chen, Liuwei Meng, Litong Wang, Xixi Huang, Shujat Ali, Xiaojing Chen, Mingen Yu, Ming Yi, Limin Li, Xi Chen, Leiming Yuan, Wen Shi, Guangzao Huang
Summary: The newly developed SERS-based LFIA strip using GERTs achieved highly sensitive detection of anti-SARS-CoV-2 IgM and IgG, with detection limits 100 times lower compared to conventional LFIA strips, improving the accuracy and reliability of early diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Oncology
Maimoona Tahira, Haq Nawaz, Muhammad Irfan Majeed, Nosheen Rashid, Shaheera Tabbasum, Muhammad Abubakar, Shamsheer Ahmad, Saba Akbar, Saba Bashir, Muhammad Kashif, Saqib Ali, Hamza Hyat
Summary: The study demonstrates that SERS combined with multivariate data analysis methods can effectively diagnose different stages of typhoid fever, including early acute and late acute stages.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2021)
Article
Biophysics
Chang-Yue Chiang, Tze-Ta Huang, Chih-Hui Wang, Chun-Jen Huang, Tsung-Heng Tsai, Sung-Nien Yu, Ying-Ting Chen, Shih-Wei Hong, Chia-Wei Hsu, Ting-Chou Chang, Lai-Kwan Chau
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Biochemical Research Methods
Wen-Yu Chen, Chih-Hsien Wang, Kai-Hao Wang, Yuh-Ling Chen, Lai-Kwan Chau, Shau-Chun Wang
Article
Integrative & Complementary Medicine
Yuan-Chiang Chung, Ying- Chen, Chih-Ming Lin, Su-Wei Chang, Tai-Hao Hsu, Wai-Jane Ho, Jaug-Geng Lin, Shih-Liang Chang, Chung-Yuh Tzeng
ACUPUNCTURE IN MEDICINE
(2020)
Article
Biochemical Research Methods
Loan Thi Ngo, Wei-Kai Wang, Yen-Ta Tseng, Ting-Chou Chang, Pao-Lin Kuo, Lai-Kwan Chau, Tze-Ta Huang
Summary: A new biosensing method utilizing MutS protein and fiber optic particle plasmon resonance (FOPPR) sensing system has been developed to detect gene mutations with high sensitivity (limit of detection down to 0.49 nM) and short analysis time (as short as 15 min). Validation using patient samples has shown accurate determination of allelic fraction, making this method a promising tool for detecting single nucleotide polymorphisms in biological samples at point-of-care sites.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Chien-Hsing Chen, Chang-Yue Chiang, Chin-Wei Wu, Chien-Tsung Wang, Lai-Kwan Chau
Summary: A novel fiber optic particle plasmon resonance biosensing platform for immunoglobulin G (IgG) detection was developed using a gold nanoparticle/graphene oxide composite as a signal amplification element. The sensor showed good analytical performance with a detection limit of 0.038 ng/mL and a linear relationship between sensor response and analyte concentrations. The sensitivity was attributed to the synergistic effect between graphene oxide and gold nanoparticles, with good reproducibility demonstrated for IgG detection.
Correction
Biochemical Research Methods
Loan Thi Ngo, Wei-Kai Wang, Yen-Ta Tseng, Ting-Chou Chang, Pao-Lin Kuo, Lai-Kwan Chau, Tze-Ta Huang
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Shuo-Feng Wang, Wen-Yu Lin, Jia-Shan Dai, Yen-Ling Chen, Yung-fou Chen, Lai-Kwan Chau
Summary: A gold nanoparticle-based SALDI-MS method was developed for the analysis of amphetamine-type stimulants (ATS) through derivatization, which significantly increased ion signal intensities. The method was successfully applied to urine samples for the determination of MA and MDMA, demonstrating improved sensitivity and validation.
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
(2021)
Article
Nanoscience & Nanotechnology
Shu-Mei Fan, Chang-Yue Chiang, Yen-Ta Tseng, Tsung-Yan Wu, Yen-Ling Chen, Chun-Jen Huang, Lai-Kwan Chau
Summary: The introduced fast and ultrasensitive biosensing method for detecting highly toxic mercury ions relies on fiber optic particle plasmon resonance and a selective molecular beacon. It offers a wide linear range and an extremely low detection limit. The method is suitable for point-of-use applications due to its low-cost instrumentation, ease of use, and portability.
ACS APPLIED NANO MATERIALS
(2021)
Article
Engineering, Chemical
Shi-Wen Chen, Tran Thi Anh Hong, Chun-Tung Chiang, Lai-Kwan Chau, Chun-Jen Huang
Summary: Organosilanes have been widely used in sol-gel material synthesis, surface functionalization, and nanotechnology. However, the limitations of organosilane have led to the emerging interest in silatrane, which offers unique tricyclic caged structure and chemical stability. This study demonstrated the application of silatrane in modifying silicon oxide substrates, achieving thin and uniform coatings. The functionalization of silatrane with polymers and immobilization of gold nanoparticles were also successfully achieved. The results showed superior protein repellency of MPS-PEGMA films compared to MPTMS-PEGMA films, and the cluster size of gold nanoparticles on APS films increased significantly after acid treatment.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2022)
Article
Chemistry, Analytical
Han-Long Liu, Yen-Ta Tseng, Mei-Chu Lai, Lai-Kwan Chau
Summary: In this study, a fiber optic nanogold-linked immunosorbent assay (FONLISA) method was developed for the rapid, sensitive, and low-cost detection of the important blood biomarker for heart failure, N-terminal pro-brain natriuretic peptide (NT-proBNP). The method utilizes a sandwich immunoassay approach and detects the analyte through changes in light intensity caused by AuNPs on the fiber core surface. The method exhibits a wide dynamic range and a low limit of detection, and shows good correlation with a commercial laboratory-based method. This method has the potential to be used as a point-of-care testing tool.
Article
Chemistry, Analytical
Ting-Chou Chang, Aileen Y. Sun, Yu-Chung Huang, Chih-Hui Wang, Shau-Chun Wang, Lai-Kwan Chau
Summary: The global pandemic of COVID-19 has driven the demand for sensitive and rapid point-of-care testing methods. This study introduces a fiber optic particle plasmon resonance biosensor that utilizes a single-stranded DNA aptamer to detect the SARS-CoV-2 nucleocapsid protein with a 15-minute detection time and a limit of detection of 2.8 nM.
Article
Chemistry, Analytical
Yuan-Yu Chen, Chih-Lu Wu, Chia-Wei Hsu, Chih-Hui Wang, Chung-Rui Su, Chun-Jen Huang, Hau-Ren Chen, Lai-Kwan Chau, Shau-Chun Wang
Summary: A fast and label-free fiber optic particle plasmon resonance (FOPPR) immunosensing method was developed to detect nervous necrosis virus (NNV) in high-value economic aquatic species. With an enhancement method, it showed high sensitivity and potential as an outbreak surveillance tool.
Article
Chemistry, Analytical
Yi-Chen Chen, Yu-Chen Chou, Jui-Han Chang, Li-Ting Chen, Chun-Jen Huang, Lai-Kwan Chau, Yen-Ling Chen
Summary: A dual-functional gold-iron oxide core-satellite hybrid nanoparticle structure is proposed as a signal reporter and preconcentrator for low abundance analytes in a nanoplasmonic biosensor, demonstrating an 18-fold improvement in limit of detection for detecting DNA compared to traditional methods. This easy-to-prepare structure shows promise for high-sensitivity and low-cost point-of-care applications.
Article
Materials Science, Multidisciplinary
Duc Tu Vu, Thanh Thu Vu-Le, Van Nghia Nguyen, Quoc Minh Le, Churng-Ren Chris Wang, Lai-Kwan Chau, Tzyy-Schiuan Yang, Michael W. Y. Chang, Cheng- Lee, Chu-Chi Ting, Jiunn-Yuan Lin, Hung-Chih Kan, Chia Chen Hsu
Summary: In this study, a multifunctional nanocomposite was developed for effective treatment of oral cancer cells, demonstrating fluorescence labeling, temperature sensing, and photothermal functions. The temperature-dependent upconversion luminescence property was utilized to determine local temperature, and the nanocomposite particles successfully labeled cancer cells on the surface.
INTERNATIONAL JOURNAL OF SMART AND NANO MATERIALS
(2021)
Article
Medicine, General & Internal
Chen-Cheng Huang, Jin-Ming Hwang, Jen-Hsiang Tsai, Jing Huei Chen, Ho Lin, Geng-Jhih Lin, Hsin-Ling Yang, Jer-Yuh Liu, Chiou-Ying Yang, Je-Chiuan Ye
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2020)