Review
Medicine, General & Internal
Kanny Diallo, Vitalis F. Feteh, Lilian Ibe, Martin Antonio, Dominique A. Caugant, Mignon du Plessis, Ala-Eddine Deghmane, Ian M. Feavers, Katya Fernandez, LeAnne M. Fox, Charlene M. C. Rodrigues, Olivier Ronveaux, Muhamed-Kheir Taha, Xin Wang, Angela B. Brueggemann, Martin C. J. Maiden, Odile B. Harrison
Summary: Bacterial meningitis is a significant global cause of morbidity and mortality, and rapid identification of the causative agent is crucial for clinical management and disease prevention. Molecular diagnostic assays have been developed as an alternative to microbiological culture, allowing for faster and more accurate detection of the pathogens causing bacterial meningitis.
Article
Endocrinology & Metabolism
Jie Yao, Wei Tang, Juan Cheng, Xin Li, Qiang Zhou
Summary: This study compared the application value of polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), and bacterial culture in diagnosing bacillary dysentery. The results showed that PCR and LAMP had higher diagnostic accuracy than bacterial culture. This study confirmed that PCR and LAMP had better specificity and sensitivity in detecting Shigella.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Biotechnology & Applied Microbiology
Claudia Gimenez, Mirna L. Sanchez, Hugo A. Valdez, Maria E. Rodriguez, Mariano Grasselli
Summary: This paper analyzes disposable micropipette tips as reagent holders in PCR analysis, showing their potential for simplifying PCR analysis and reducing contamination. The study proves that the detection of Bordetella pertussis can be quickly done using functional tips.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yair E. Gatt, Hanah Margalit
Summary: Within-host adaptation in chronic bacterial infections involves substantial genomic changes. Different species share common adaptive strategies, often driven by the host immune system. The ubiquity of adaptive strategies across species reveals key evolutionary changes.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biotechnology & Applied Microbiology
Sumeyra Vural Kaymaz, Ali F. Ergenc, Ali O. Aytekin, Stuart J. Lucas, Meltem Elitas
Summary: We developed a simple, portable, low-cost, colorimetric, and remotely controllable platform for reliable, high-throughput, and rapid diagnosis using loop-mediated isothermal amplification (LAMP) assays. This platform is capable of performing nucleic acid isolation and detection from sampling, and we confirmed its specificity and sensitivity through experiments.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Multidisciplinary Sciences
Muhammad Tayyab, Donal Barrett, Gijs van Riel, Shujing Liu, Bjorn Reinius, Curt Scharfe, Peter Griffin, Lars M. Steinmetz, Mehdi Javanmard, Vicent Pelechano
Summary: This article presents a fast and accurate method for nucleic acid detection, combining the generation of DNA nanoballs with electrical impedance measurement. The method is flexible for detecting various DNA/RNA targets and has the potential to be integrated into an inexpensive, sensitive, and rapid standalone device.
Article
Plant Sciences
Monika Kaluzna, Andjelka Prokic, Aleksa Obradovic, William A. Weldon, Virginia O. Stockwell, Joel F. Pothier
Summary: In this study, a comparative genomics approach was used to identify unique sequences in the publicly available genomes of Xanthomonas arboricola pv. corylina (Xac). Based on these unique sequences, new molecular diagnostic tools were designed for the rapid and accurate detection and identification of Xac.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Microbiology
Yoong-Ling Oon, Yoong-Sin Oon, Muhammad Ayaz, Min Deng, Lu Li, Kang Song
Summary: This comprehensive review examines the trajectory of waterborne pathogenic bacteria detection technologies from traditional techniques to current detection methods including PCR techniques and emerging techniques such as biosensors and artificial intelligence. The paper discusses the merits, constraints, research gaps, and future perspectives of these techniques, with a focus on their applicability in field settings and timely analyses. The integration of AI with these technologies has enhanced detection accuracy, enabling real-time analysis of large datasets.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Sedef Zeliha Oner, Ilknur Kaleli, Melek Demir, Ergun Mete, Ahmet Caliskan
Summary: This study aimed to evaluate the bacterial and viral causes of central nervous system (CNS) infection using multiplex PCR. The results showed that using a comprehensive bacterial multiplex PCR panel containing common pathogens could be more effective in pathogen detection. Care should be taken when interpreting the viral multiplex PCR results.
JCPSP-JOURNAL OF THE COLLEGE OF PHYSICIANS AND SURGEONS PAKISTAN
(2022)
Review
Microbiology
Linoj Samuel
Summary: Blood cultures have been the main tool in clinical microbiology laboratories for a long time, but there are still limitations in identifying the causative agent in septic patients. While molecular technologies have revolutionized various areas of clinical microbiology, they have not yet provided a viable alternative to blood cultures. Recently, there has been increased interest in utilizing new approaches to address this challenge.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Plant Sciences
Darko Voncina, Alfredo Diaz-Lara, Darko Preiner, Maher Al Rwahnih, Kristian Stevens, Snjezana Juri, Nenad Malenica, Silvio Simon, Baozhong Meng, Edi Maletic, Hrvoje Fulgosi, Bogdan Cvjetkovic
Summary: Grapevine collections are crucial for studying viruses and virus-like pathogens. The collection of Croatian autochthonous grapevine cultivars has shown high infection rates and virome diversity. However, maintaining the collection is challenging due to the recent spread of flavescence doree and susceptibility to various abiotic factors.
Article
Multidisciplinary Sciences
Xiaoqing Tong, Jiayi Wu, Li Mei, Yongjun Wang
Summary: This study investigated the dieback and basal stem necrosis disease in Chinese hickory plantations in China, resulting in a high incidence rate. Through molecular detection and loop-mediated isothermal amplification technology, a convenient tool was developed for field monitoring of the pathogenic Phytophthora cinnamomi.
Article
Chemistry, Analytical
M. G. Rizzo, S. Carnazza, L. M. De Plano, D. Franco, M. S. Nicol, V Zammuto, S. Petralia, Giovanna Calabrese, C. Gugliandolo, S. Conoci, S. P. P. Guglielmino
Summary: The study developed a new method for rapid and sensitive detection of bacteria in blood using engineered M13 phages and PCR in a miniaturized silicon microchip, providing a fast and efficient diagnosis approach.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Microbiology
Jorge Oyhenart, Mariana Morero
Summary: An affordable and portable instrument called CELDA, controlled by Arduino, was used for rapid and accurate detection of Tritrichomonas foetus DNA in less than 60 minutes. It outperformed visual inspection in distinguishing positive samples.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Yuying Si, Tong Zhang, Nianzhen Chen, Yu Cheng, Lan Wang, Jiayi Yuan, Gen Li, Ming Zong, Guodong Sui, Lieying Fan
Summary: The study developed an extraction-free LAMP method for rapid detection of eight lower respiratory pathogens, showing high consistency with sequencing methods.
Through analysis of 528 samples, the LAMP assay demonstrated higher sensitivity than standard culture methods, with high specificity and sensitivity in diagnosing LRTIs.
JOURNAL OF MICROBIOLOGICAL METHODS
(2021)