Article
Infectious Diseases
Maristela Peckle Peixoto, Hermes Ribeiro Luz, Daniel Paiva Barros de Abreu, Joao Luiz Horacio Faccini, Douglas McIntosh
Summary: This study reports the detection of a novel Rickettsia bacterium, designated as Rickettsia sp. Itingu??, infecting larvae of the argasid tick Ornithodoros faccinii collected from toad Rhinella ornata in Southeastern Brazil. The bacterium belongs to the spotted fever group (SFG) of Rickettsia, closely related to Rickettsia massiliae and Rickettsia helvetica subgroups.
TICKS AND TICK-BORNE DISEASES
(2021)
Article
Microbiology
Hamid Staji, Mohammadhasan Yousefi, Mahmoud Ahmadi Hamedani, Iradj Ashrafi Tamai, Sahar Ghaffari Khaligh
Summary: This study reported the first occurrence of A. capra in wild onagers and suggested that equines could be infected with and act as reservoirs for A. capra.
VETERINARY MICROBIOLOGY
(2021)
Article
Plant Sciences
Jiao-Sheng Li, Luo-Yu Wu, Hui Zhang, Xiu-Shi Song, Jian-Xin Wang, Ming-Guo Zhou, Yi-Ping Hou
Summary: Phenamacril is a fungicide that effectively controls wheat scab caused by Fusarium spp. Through PCR-RFLP, three common mutations in the myosin-5 gene can be detected in F. graminearum with resistance to phenamacril. This method showed a 95.12% accuracy in detecting resistant strains compared to conventional methods.
Article
Infectious Diseases
Yaqun Yan, Chenyang Lu, Pihong Gong, Zhiyang Pei, Yongshuai Peng, Fuchun Jian, Rongjun Wang, Longxian Zhang, Meng Qi, Changshen Ning
Summary: This study aimed to investigate the prevalence and phylogeny of A. phagocytophilum and related strains in small ruminants and ticks in China. The results showed that A. phagocytophilum-like 1 and 2 variants were widespread in sampled animals, with prevalence rates of 22.6% and 0.7% respectively. Phylogenetic analysis based on the groEL gene revealed distinct clades for A. phagocytophilum-like 1 and 2 strains, related to Japanese and Chinese strains of A. phagocytophilum.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Multidisciplinary Sciences
Shanshan Zhang, Qian Yang, Songzhe Fu, Colin R. Janssen, Mieke Eggermont, Tom Defoirdt
Summary: This study demonstrates that indole signaling has a significant impact on the virulence of Vibrio tasmaniensis LGP32 and Vibrio crassostreae J2-9, affecting their motility, biofilm formation, and gene expression. Pretreatment with indole decreases bacterial virulence towards mussel larvae, indicating that indole signaling could be a promising target for antivirulence therapy.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Asmaa Magouz, Ismail El-Kon, Enrique Raya-alvarez, Enas Khaled, Noura Alkhalefa, Alaa S. Alhegaili, Manal F. El-khadragy, Ahmad Agil, Ehab Kotb Elmahallawy
Summary: This study reports the prevalence and molecular analysis of CPV-2 variants collected from El-Gharbia and Kafrelsheikh governorates in the Delta of Egypt. The results showed that CPV-2a and CPV-2c are circulating in the study area.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Chemistry, Analytical
Tugba Yilmaz, Edgar D. Goluch
Summary: Cholera is a global health problem with over 143,000 deaths annually. Contaminated water sources and early diagnosis of suspected cholera cases are crucial in preventing the spread of the disease. New analytical tools are needed for faster and more efficient detection of Vibrio cholerae.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Agronomy
Weier Cui, Alan Zamorano, Nicolas Quiroga, Assunta Bertaccini, Nicola Fiore
Summary: Research has shown that the SSU12p and LSU36p genes are reliable additional markers for detection and differentiation of phytoplasmas.
PHYTOPATHOLOGIA MEDITERRANEA
(2021)
Article
Immunology
Yusuke Ota, Reina Okada, Hideyuki Takahashi, Ryoichi Saito
Summary: This study established a mismatched PCR-restriction fragment length polymorphism (RFLP) assay for detecting mutations in gyrA associated with ciprofloxacin-resistant Neisseria meningitidis. The assay showed correlation with gyrA sequencing and has the potential to contribute to appropriate antibiotic selection for meningococcal chemoprophylaxis and prevent the spread of ciprofloxacin-resistant N. meningitidis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Medicine, General & Internal
Kanny Diallo, Vitalis F. Feteh, Lilian Ibe, Martin Antonio, Dominique A. Caugant, Mignon du Plessis, Ala-Eddine Deghmane, Ian M. Feavers, Katya Fernandez, LeAnne M. Fox, Charlene M. C. Rodrigues, Olivier Ronveaux, Muhamed-Kheir Taha, Xin Wang, Angela B. Brueggemann, Martin C. J. Maiden, Odile B. Harrison
Summary: Bacterial meningitis is a significant global cause of morbidity and mortality, and rapid identification of the causative agent is crucial for clinical management and disease prevention. Molecular diagnostic assays have been developed as an alternative to microbiological culture, allowing for faster and more accurate detection of the pathogens causing bacterial meningitis.
Review
Fisheries
Ke-Yan Loo, Jodi Woan-Fei Law, Loh Teng-Hern Tan, Priyia Pusparajah, Vengadesh Letchumanan, Learn-Han Lee
Summary: This review summarizes various rapid detection methods for Vibrio sp., including polymerase chain reactions, loop-mediated isothermal amplification, enzyme-linked immunosorbent assays, microarrays, lateral flow immunoassays, aptasensors, spot dye-based sensors, and CRISPR/Cas-PCR systems. These rapid detection methods have shown high specificity and sensitivity in detecting various species within the genus Vibrio. However, each method has its own advantages and limitations, highlighting the need for continuous improvement and development of techniques with high sensitivity, specificity, affordability, and minimal effort for consistent and accurate diagnoses.
Article
Plant Sciences
Meng Ni, Xiaofang Yi, Qin Wang, Juan Wang, Shuang Wang, Liwang Liu, Liang Xu, Yan Wang
Summary: The study identified S haplotypes among 79 cultivated radish genotypes by analyzing the gene sequence and using PCR-RFLP method. The results showed that S haplotypes could be divided into Class I, Class II, and Class I/II, and three new haplotypes were identified in radish. By digestion of DNA fragments, homozygous S haplotypes could be easily distinguished. The molecular identification was consistent with field pollination and pollen tube germination results.
Article
Agriculture, Dairy & Animal Science
Vilas B. Dongre, Sudhakar P. Awandkar, Vishwas M. Salunke, Mahesh B. Kulkarni, Laxmikant S. Kokate, Shyam B. Kale, Ravji R. Mugale
Summary: This study investigated the allelic and genotypic frequencies in the coding region of the TYR gene, as well as gene expression profile, in Deoni animals of western India. The results showed that the genotypic frequency of the TYR gene was not significant across different strains, genders, age groups, and levels of partial albinism. The gene expression of tyrosinase was significantly higher in young animals compared to adult animals, and it was higher in animals with low intensity of partial albinism.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Sishuo Song, Fan Ji, Qingdong Zeng, Xiaoteng Ding, Jiahao Lin, Xinsheng Wang, Yuanhong Xu, Ruijie Deng, Jinghong Li
Summary: Fungicide abuse can lead to the emergence of fungicide-resistant fungal pathogens, posing a threat to agriculture and food safety. In this study, researchers developed iARMS, an isothermal amplification refractory mutation system, which enables rapid and sensitive detection of fungicide-resistant crop fungal pathogens. The iARMS assay showed high specificity and sensitivity, and it was able to detect rare fungicide-resistant isolates.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ritong Sun, Jiao Chen, Yingeng Wang, Zheng Zhang, Yong Li, Fengmei Li, Cuiping Ma, Qingxia Han, Yanjing Shi
Summary: A highly sensitive and specific real-time narrow thermal-cycling amplification detection method based on accelerated strand exchange amplification (ASEA) was developed for the detection of Vibrio parahaemolyticus. The method can detect low concentrations of Vibrio parahaemolyticus in cultured samples and artificially spiked scallop meat within a short time. It is a useful tool for rapid detection of Vibrio parahaemolyticus in seafood samples.
ANALYTICAL METHODS
(2023)
Article
Ecology
Ajin Madhavan, Reshma Silvester, M. P. Prabhakaran, Reza Naderloo, C. K. Radhakrishnan, N. R. Menon
REGIONAL STUDIES IN MARINE SCIENCE
(2020)
Article
Chemistry, Analytical
Rekha Mol Kollakalnaduvil Raghavan, Manzur Ali Pannippara, Sapna Kesav, Abraham Mathew, Sarita G. Bhat, Mohamed A. A. Hatha, K. K. Elyas
Summary: The extracellular thermostable antibacterial peptide MFAP9 was purified from marine Aspergillus fumigatus BTMF9, demonstrating strong antibacterial activity and antibiofilm effect against various bacteria. It showed no toxicity to human red blood cells, indicating its potential as a novel anti-infective agent.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biotechnology & Applied Microbiology
T. Jabir, P. V. Vipindas, K. P. Krishnan, A. A. Mohamed Hatha
Summary: This study investigated the abundance and diversity of diazotrophs in Arctic fjord sediments, revealing dominance of δ-proteobacterial diazotrophs and significant correlations with TOC/TN ratio and total organic carbon content. Glacier runoff introducing low nitrogen content organic matter enhances the abundance and diversity of nitrogen-fixing microorganisms in the fjord sediments.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
T. Jabir, P. V. Vipindas, Y. Jesmi, P. S. Divya, B. M. Adarsh, H. S. Nafeesathul Miziriya, A. A. Mohamed Hatha
Summary: Benthic nitrogen fixation in tropical estuaries, particularly during the post-monsoon period, plays a significant role in the marine nitrogen cycle. Sulfur reducing bacterial phylotypes are major diazotrophs contributing to nitrogen fixation in these systems.
MARINE POLLUTION BULLETIN
(2021)
Article
Ecology
Ally C. Antony, Reshma Silvester, P. S. Divya, P. A. Aneesa, Bini Francis, Ajith C. Joseph, Midhun Shah Hussain, B. T. Umesh, Joy George, Mohamed Hatha Abdulla
Summary: The study evaluated the seasonal prevalence of faecal coliforms in Indian black clam, sediment and harvesting waters along the Cochin estuary. E. coli strains were isolated and identified, with a high prevalence during monsoon and post-monsoon seasons. Pathogenic serotypes were present in the isolates, highlighting potential health hazards to consumers.
REGIONAL STUDIES IN MARINE SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
S. Vishnupriya, T. Jabir, K. P. Krishnan, A. A. Mohamed Hatha
Summary: Kongsfjorden, an Arctic fjord, is experiencing changes in bacterial community structure of sediments due to glacier melt and Atlantification driven by accelerated warming in the Arctic. The dominant phyla in the sediments are Proteobacteria, Bacteroidetes, and Epsilonbacteraeota, with alpha- and gamma-Proteobacteria class dominating in surface sediments and delta-Proteobacteria in subsurface sediments. Total organic carbon is suggested to be a major factor affecting bacterial diversity, and bacterial communities are predicted to be involved in the degradation of complex organic compounds and biosynthesis of secondary metabolites.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Ecology
A. A. Mohamed Hatha, T. Jabir, E. Akhil Prakash, K. P. Krishnan
Summary: Microcosm experiments were conducted to study the relative survival of two Escherichia coli strains in the Kongsfjorden water during the 2017 Arctic summer. Biotic factors and solar radiation were found to be most effective in removing E. coli from the water, while the chemical composition of the fjord water supported extended survival of the bacteria.
Article
Environmental Sciences
Reshma Silvester, Ajin Madhavan, Aswin Kokkat, Ajilesh Parolla, B. M. Adarsh, M. Harikrishnan, Mohamed Hatha Abdulla
Summary: The global emergence of multi-drug resistant and hypervirulent strains of Klebsiella pneumoniae poses significant challenges to current treatment strategies and public health management in Low-and Middle-Income Countries. Regular surveillance and necessary interventions are strongly recommended to address this critical issue.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Ecology
Preetha Gopalakrishnan Nair, Shoji Joseph, Narayana Pillai, Mohamed Hatha Ammanaveetil Abdulla
Summary: This study examines the relationship between recurrent Potential Fishing Zones (PFZ) and seasonal hydrographic conditions along the Southwest coast of India. The findings suggest that the frequency and location of repeat PFZs are influenced by the region's predominant hydrographic settings, particularly during the Northeast Monsoon season.
REGIONAL STUDIES IN MARINE SCIENCE
(2022)
Review
Environmental Sciences
Jasna Vijayan, Vinod Kumar Nathan, Parvathi Ammini, Abdulla Mohamed Hatha Ammanamveetil
Summary: Microbial analysis is crucial in aquatic ecology for assessing the role of microbes in food web dynamics and biogeochemical processes. Traditional methods like microscopy and culture-based analysis have been gradually replaced by molecular techniques, especially metagenomics analysis which allows the identification and characterization of microorganisms and their functionality. This review focuses on the metagenomics analysis of bacterial diversity in various aquatic systems in India.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Reshma Silvester, Amala Saji, Anagha Rani Divakaran, P. M. Dilshana, Radha Nair, Mohamed Hatha, M. Harikrishnan
Summary: India is a top supplier of value-added seafood, with Cochin being a leading fishing port contributing to the country's major seafood export. Research shows an increase in the prevalence of multi-drug resistant Vibrio parahaemolyticus in shellfish, posing a significant threat to human health. Strong emphasis is placed on practicing complete hygiene and adequate cooking to prevent the dissemination of pathogenic V. parahaemolyticus in seafood and the potential risk of human infection.
ANNALS OF ANIMAL SCIENCE
(2022)
Article
Chemistry, Medicinal
P. F. Steffi, M. Pragathi, P. F. Mishel, B. Thamilmaraislvi, S. Reshma
Summary: Onychomycosis is a common nail infection caused by fungi, primarily affecting farmers aged between 16-30 years. The infection is attributed to farmers spending a significant amount of time in the field engaging in various agricultural activities. A study aimed to formulate an antifungal cream using traditional herbs to treat the infection, with positive results showing good consistency in the formulation.
INTERNATIONAL JOURNAL OF LIFE SCIENCE AND PHARMA RESEARCH
(2021)
Article
Fisheries
Ally C. Antony, Reshma Silvester, P. A. Aneesa, Bini Francis, Mohamed Hatha Abdulla
Summary: The study evaluated the efficacy of a closed water depuration system for Indian black clams, showing complete removal of fecal coliforms and fecal streptococci within 48 hours, but total coliforms were not fully depurated even after 96 hours. The system uses simple, cost-effective, natural, and renewable water treating agent such as coconut shell-based activated carbon and is suitable for household purposes.
FISHERY TECHNOLOGY
(2021)
Article
Fisheries
Reshma Silvester, Ally C. Antony, Jesmi Yousuf, Ajin Madhavan, P. M. Sooria, Aswin Kokkat, M. Harikrishnan, Mohamed Hatha
Summary: The study found that pathogenic Vibrio species have prolonged and better survival in estuarine sediments compared to water, indicating sediments may act as a permanent reservoir of the bacterium. Biological factors and chemical composition of estuarine water play a significant role in the removal of Vibrios from the environment. Exposure to sunlight has a deleterious effect on the survival of these organisms.
FISHERY TECHNOLOGY
(2021)
