Journal
MICROBIAL PATHOGENESIS
Volume 107, Issue -, Pages 12-16Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2017.03.011
Keywords
LPS; TLR4; Bovine mammary epithelial cells
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Funding
- National Natural Science Foundation of China [31602122, 30771596]
- Graduate Innovation Fund of Jilin University [2016114]
- China Postdoctoral Science Foundation [2016M600233]
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Mastitis is an inflammatory reaction caused by microorganisms in the mammary gland which usually leads to the decrease of the dairy production. It vastly makes bad effect on the cattle industry all over the world. Nowadays, an increasing number of scientists keep a watchful eye on natural compounds to prevent mastitis. Sodium houttuyfonate (SH) has been reported to have antibacterial and anti-inflammatory effects. This experiment aimed to investigate the anti-inflammatory properties of SH on LPS-stimulated primary bovine mammary epithelial cells (bMEC). The effects of SH on TNF-alpha, IL-1 beta, and IL-6 production were detected by qRT-PCR. Western blot analysis was used for detecting the effects of SH on TLR4/NF-kappa B signal pathways. The results showed that SH significantly inhibited LPS-stimulated TNF-alpha, IL-1 beta and IL-6 production. Furthermore, SH significantly inhibited LPS-induced TLR4 expression and NF-kappa B activation. In summary, these results suggested that SH inhibited LPS-induced inflammatory response by inhibiting TLR4/NF-kappa B signaling pathway. SH is a potential agent for the treatment of mastitis. (C) 2017 Elsevier Ltd. All rights reserved.
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