Journal
MICROBIAL PATHOGENESIS
Volume 107, Issue -, Pages 88-97Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2017.03.019
Keywords
Plectranthus barbatus; Ceriodaphnia cornuta; Bioaccumulation; Zn2+ dissolution; Antibacterial; Antibiofilm; ZnO nanoparticles
Categories
Funding
- Department of Biotechnology (DBT), New Delhi, India [BT/PR7903/AAQ/3/638/2013]
- DST, New Delhi, India under INSPIRE programme [IF140145]
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Zinc oxide nanoparticles were synthesized using the aqueous leaf extracts of Plectranthus barbatus (Plb-ZnO NPs) and characterized by UV visible spectroscopy, XRD, FUR, SEM and EDS. UV Visible spectra recorded the absorbance peak of Plb-ZnO NPs at 343 nm. SEM analyses showed the spherical shape of Plb-ZnO NPs with the particle size between 30 and 60 nm. Plb-ZnO NPs exhibited antibacterial and antibiofilm activity against Gram positive Bacillus subtilis at all tested concentrations. In contrast, Plb-ZnO NPs showed antibacterial and antibiofilm activity against Gram negative Vibrio parahaemolyticus and Proteus vulgaris only at 100 mu g/ml. The Atomic absorption spectrometry (AAS) revealed that Zn2+ dissolution was 1.87 and 8.8 mu g/L at 10 and 160 mu g/L of Plb-ZnO NPs respectively. The body accumulation of Zn2+ was increased from 0.8 mu g/g body weight to 3.5 mu g/g body weight when C. cornuta exposed to 10 mu g/L and 160 mu g/L. respectively. Plb-ZnO NPs were toxic to Ceriodaphnia cornuta neonates (LC50: 28 mu g/L). Plb-ZnO NPs caused 100% mortality of C cornuta at 160 mu g/L after 24 h. However, zinc acetate does not cause any mortality of C cornuta upto 350 mu g/L. The light and confocal laser scanning microscopic images evidenced the uptake and accumulation of Plb-ZnO NPs on the internal gut regions of C cornuta at 160 mu g/L after 5, 10, 15, 20 and 24 h. Abnormalities in the swimming behaviour such as erratic swimming (ERR), migration to bottom (BOT) and migration to water surface (SUR) of C cornuta were noticed after treatment with different concentrations of Plb-ZnO NPs. (C) 2017 Elsevier Ltd. All rights reserved.
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