Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica

Genome and methylome of the oleaginous diatom Cyclotella cryptica reveal genetic flexibility toward a high lipid phenotype

(2016) Jesse C. Traller et al.   Biotechnology for Biofuels

Transcript level coordination of carbon pathways during silicon starvation-induced lipid accumulation in the diatomThalassiosira pseudonana

(2016) Sarah R. Smith et al.   NEW PHYTOLOGIST

Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana

(2016) Amanda Hopes et al.   Plant Methods

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

Robust expression of heterologous genes by selection marker fusion system in improved Chlamydomonas strains

(2015) Fantao Kong et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Improved and versatile viral 2A platforms for dependable and inducible high-level expression of dicistronic nuclear genes in Chlamydomonas reinhardtii

(2015) Thomas M. Plucinak et al.   PLANT JOURNAL

Designer diatom episomes delivered by bacterial conjugation

(2015) Bogumil J. Karas et al.   Nature Communications

Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway

(2015) Jae Seong Lee et al.   Scientific Reports

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size

(2014) Henning Kirst et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Evidence for a Regulatory Role of Diatom Silicon Transporters in Cellular Silicon Responses

(2014) Roshan P. Shrestha et al.   EUKARYOTIC CELL

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

5′-coding sequence of thenasAgene ofAzotobacter vinelandiiis required for efficient expression

(2014) Baomin Wang et al.   FEMS MICROBIOLOGY LETTERS

Antenna size reduction as a strategy to increase biomass productivity: a great potential not yet realized

(2014) Tim de Mooij et al.   JOURNAL OF APPLIED PHYCOLOGY

Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses

(2014) Beth A. Rasala et al.   PHOTOSYNTHESIS RESEARCH

Efficient translation initiation dictates codon usage at gene start

(2013) K. Bentele et al.   Molecular Systems Biology

High throughput imaging to the diatom Cyclotella cryptica demonstrates substantial cell-to-cell variability in the rate and extent of triacylglycerol accumulation

(2013) Jesse C. Traller et al.   Algal Research-Biomass Biofuels and Bioproducts

Whole transcriptome analysis of the silicon response of the diatom Thalassiosira pseudonana

(2012) Roshan Shrestha et al.   BMC GENOMICS

An engineered diatom acting like a plasma cell secreting human IgG antibodies with high efficiency

(2012) Franziska Hempel et al.   Microbial Cell Factories

A generalised module for the selective extracellular accumulation of recombinant proteins

(2012) Yanina R Sevastsyanovich et al.   Microbial Cell Factories

Silencing of the Violaxanthin De-Epoxidase Gene in the Diatom Phaeodactylum tricornutum Reduces Diatoxanthin Synthesis and Non-Photochemical Quenching

(2012) Johann Lavaud et al.   PLoS One

Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide

(2012) Beth A. Rasala et al.   PLoS One

The place of diatoms in the biofuels industry

(2012) Mark Hildebrand et al.   Biofuels-UK

Nickel and low CO2-controlled motility in Chlamydomonas through complementation of a paralyzed flagella mutant with chemically regulated promoters

(2011) Paola Ferrante et al.   BMC PLANT BIOLOGY

A new inducible expression system in a transformed green alga, Chlorella vulgaris

(2011) Y.F. Niu et al.   GENETICS AND MOLECULAR RESEARCH

Microalgae as bioreactors for bioplastic production

(2011) Franziska Hempel et al.   Microbial Cell Factories

Modifications of the metabolic pathways of lipid and triacylglycerol production in microalgae

(2011) Wei-Luen Yu et al.   Microbial Cell Factories

Algae as Protein Factories: Expression of a Human Antibody and the Respective Antigen in the Diatom Phaeodactylum tricornutum

(2011) Franziska Hempel et al.   PLoS One

High-level recombinant protein expression in transgenic plants by using a double-inducible viral vector

(2011) S. Werner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.

(2011) O. Kilian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Micro-algae come of age as a platform for recombinant protein production

(2010) Elizabeth Specht et al.   BIOTECHNOLOGY LETTERS

Enhanced chloroplast transgene expression in a nuclear mutant of Chlamydomonas

(2010) Laure Michelet et al.   PLANT BIOTECHNOLOGY JOURNAL

Factors effecting expression of vaccines in microalgae

(2009) Raymond Surzycki et al.   BIOLOGICALS

Generation ofChlamydomonasstrains that efficiently express nuclear transgenes

(2008) Juliane Neupert et al.   PLANT JOURNAL

Silicon Uptake in Diatoms Revisited: A Model for Saturable and Nonsaturable Uptake Kinetics and the Role of Silicon Transporters

(2007) K. Thamatrakoln et al.   PLANT PHYSIOLOGY