Journal
METABOLIC ENGINEERING
Volume 39, Issue -, Pages 1-8Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2016.11.001
Keywords
Carbon utilization; Trehalose; UDP-glucose; Glycosylation; Polysaccharide
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Funding
- College of Engineering, The University of Georgia, Athens
- International Joint Graduate-Training Program Scholarship of Beijing University of Chemical Technology
- National Natural Science Foundation of China [21376017]
- Fundamental Research Funds for the Central Universities [buctrc201613, ZY1629]
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In nature glucose is a common carbon and energy source for catabolic use and also a building unit of polysaccharides and glycosylated compounds. The presence of strong glucose catabolic pathways in microorganism rapidly decomposes glucose into smaller metabolites and challenges non-catabolic utilization of glucose as C6 building unit or precursor. To address this dilemma, we design a synergetic carbon utilization mechanism (SynCar), in which glucose catabolism is inactivated and a second carbon source (e.g. glycerol) is employed to maintain cell growth and rationally strengthen PEP driving force for glucose uptake and non catabolic utilization. Remarkably, a trehalose biosynthesis model developed for proof-of-concept indicates that SynCar leads to 131% and 200% improvement in trehalose titer and yield, respectively. The conversion rate of glucose to trehalose reaches 91% of the theoretical maximum. This work demonstrates the broad applicability of SynCar in the biosynthesis of molecules derived from non-catabolic glucose.
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