Article
Biology
Bo Lv, Xiaoyu Xu, Xunyi Zhang, Lingbin Qi, Wen He, Lu Wang, Xian Chen, Luying Peng, Jinfeng Xue, Yazhong Ji, Zhigang Xue
Summary: Human endometrial stromal cells show differential gene expression related to blood vessel development and female pregnancy after coculture with embryos, indicating the initiation of blood vessel development at the implantation stage. Key pathways including epithelial cell development and oxygen response are involved in the signaling processes during this stage. The interaction between EmSCs and embryonic trophoblasts involves integrins and fibroblast growth factor pathways.
Article
Biochemistry & Molecular Biology
Atsuya Tsuru, Mikihiro Yoshie, Junya Kojima, Ryo Yonekawa, Mana Azumi, Kazuya Kusama, Hirotaka Nishi, Kazuhiro Tamura
Summary: PGRMC1 is involved in cellular senescence during decidualization through the regulation of FOXO1 expression.
Article
Cell Biology
Elizabeth Delorme-Axford, Xin Wen, Daniel J. J. Klionsky
Summary: Macroautophagy/autophagy is a cellular degradation and recycling pathway that plays a crucial role in cell health and survival. Aberrant autophagy is involved in the development of various human diseases. The transcription factor Stb5 has been identified as a negative regulator of autophagy, and its impact on autophagy activity is primarily through its effect on genes related to cellular metabolism.
Article
Endocrinology & Metabolism
Ellen Menkhorst, Teresa So, Kate Rainczuk, Siena Barton, Wei Zhou, Tracey Edgell, Evdokia Dimitriadis
Summary: A healthy pregnancy requires successful blastocyst implantation into an adequately prepared endometrium. The decidualization of uterine endometrial stromal fibroblast cells (hESFs) is critical for a healthy pregnancy. This study investigates how decidualization affects miR release and the function of one miR, miR-19b-3p, which has been associated with recurrent pregnancy loss.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Chemistry, Applied
Cheng Li, Yiming Hu, Bin Zhang
Summary: Starch, a major macronutrient in plant-based foods, is influenced by plant cellular structures and chemical compositions, affecting its functionality. By understanding and controlling these structures, improvements in starch properties can be achieved.
FOOD HYDROCOLLOIDS
(2021)
Article
Developmental Biology
P. Deryabin, J. S. Ivanova, A. Borodkina
Summary: Successful implantation requires a finely tuned dialog between the invading embryo and the maternal endometrium. Premature senescence of endometrial stromal cells (EnSC) can lead to defective decidual transformation and impaired endometrial-blastocyst interaction. Senescent EnSC exhibit elevated intracellular reactive oxygen species (ROS) levels, originating from mitochondrial dysfunction and insufficient antioxidant defense. This imbalance in ROS release affects neighboring trophoblast-like cells, leading to DNA damage and potentially impacting embryo migration and invasion.
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Developmental Biology
Upendra Kumar Soni, Sangappa Basanna Chadchan, Rakesh Kumar Gupta, Vijay Kumar, Rajesh Kumar Jha
Summary: miR-149 plays a crucial role in regulating endometrial receptivity and decidualization for trophoblast implantation by targeting PARP-2 and caspase-8 proteins in endometrial cells.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Sabrina C. Brunetti, Michelle K. M. Arseneault, Justin A. Wright, Zhejun Wang, Mohammad-Reza Ehdaeivand, Michael J. Lowden, Jean Rivoal, Hala B. Khalil, Gajra Garg, Patrick J. Gulick
Summary: Heterotrimeric G proteins and calcium signaling both play roles in plant responses to environmental stress. The interaction between the calcium-binding protein RD20/CLO3 and the G-protein subunit GPA1 influences plant growth and development, affecting processes like hypocotyl length and leaf morphology. RD20/CLO3 acts as a negative regulator of GPA1, providing a novel insight into their functional relationship.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Rosario Macchi, Agustina D. Sotelo, Andrea C. Parrado, Luciana S. Salaverry, Guillermo A. Blanco, Marisa S. Castro, Estela B. Rey-Roldan, Andrea M. Canellada
Summary: During implantation in humans, there is a deep interaction between the endometrium and the invading trophoblast. The AT1 receptor is involved in the reciprocal dialogue occurring between endometrial stroma and trophoblast cells. Angiotensin II (Ang II) can promote endometrial decidualization and secretion of CXCL8 and MMP-2 in human endometrial stromal cells. Treatment with losartan, an AT1 receptor blocker, can inhibit the effects of Ang II on T-HESC and trophoblast migration.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2023)
Review
Health Care Sciences & Services
David M. Z. B. Hennes, Anna Rosamilia, Jerome A. Werkmeister, Caroline E. Gargett, Shayanti Mukherjee
Summary: Endometrial mesenchymal stem/progenitor cells (eMSC) show potential for applications in treating chronic and degenerative human diseases, with less invasive extraction methods and improved potency and proliferative capacity. They are particularly useful for pelvic organ prolapse treatment.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Rheumatology
Mancy Tong, Teimur Kayani, Deidre M. Jones, Jane E. Salmon, Shannon Whirledge, Lawrence W. Chamley, Vikki M. Abrahams
Summary: This study found that antiphospholipid antibodies can increase decidualization, senescence, and inflammation in endometrial stromal cells, and low molecular weight heparin can reduce this effect. These findings shed new light on the pathogenesis of pregnancy complications in women with antiphospholipid antibodies and highlight the importance of heparin in preventing miscarriage.
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Cell Biology
Li-Ling Lin, Simran Makwana, Meizhen Chen, Chiou-Miin Wang, Laurel H. Gillette, Tim H. Huang, Richard O. Burney, Bruce J. Nicholson, Nameer B. Kirma
Summary: Endometriosis is a gynecologic disorder affecting around 10% of women of reproductive age, characterized by the growth of lesions within the abdominal cavity. This study found that endometrial stromal cells from endometriosis patients undergo changes in adhesive properties and mesenchymal characteristics in response to peritoneal mesothelial cell interactions, facilitating lesion formation.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Developmental Biology
Faying Liu, Zengming Li, Jiubai Guo, Shufen Fang, Jiangyan Zhou, Bianna Cao, Jun Liu, Yulan Yi, Xiaoqun Yuan, Xiaoyun Xu, Ouping Huang, Liqun Wang, Yang Zou
Summary: The study revealed that LASPI plays an important role in the pathogenesis of adenomyosis, with its overexpression enhancing the proliferative, migratory and invasive abilities of EcESCs, potentially related to DNA hypermethylation. Further investigation is needed to explore the detailed molecular mechanisms of LASPI upregulation in promoting cell proliferation, invasion and migration in adenomyosis. LASPI could serve as a prognostic biomarker for adenomyosis.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Oncology
Weilin Zhao, Mei Cui, Ruiqi Zhang, Xihua Shen, Xin Xiong, Xinhua Ji, Lin Tao, Wei Jia, Lijuan Pang, Zhenzhu Sun, Chun Wang, Hong Zou
Summary: The differential diagnosis of endometrial stromal tumor (EST) and uterine cellular leiomyoma (CL) remains a challenge in clinical practice. IFITM1 and CD10 show high expression in EST but low expression in CL; while h-caldesmon and SMA show the opposite pattern. The combination of IFITM1, CD10, SMA, and h-caldesmon is effective for the differential diagnosis of EST and CL.
Article
Biochemistry & Molecular Biology
Roshan Kumar, Naveen C. Bisht
Summary: This study isolated homologs encoding RGS proteins from Brassica juncea and identified interacting proteins with RGS proteins. These interacting proteins play important roles in cell wall metabolism and phosphorylation-mediated cell signaling, and have a coexpression pattern with RGS homologs.
BIOSCIENCE REPORTS
(2022)
Article
Developmental Biology
Jose Martin Murrieta-Coxca, Leonie Aengenheister, Astrid Schmidt, Udo R. Markert, Tina Buerki-Thurnherr, Diana M. Morales-Prieto
Summary: The physical connection between mother and offspring during pregnancy allows for bi-directional exchange of cells, which can persist long-term in the recipient individual and play a role in immune adaptation and tolerogenic responses. However, the mechanisms of cell transfer across the placenta barrier are still poorly understood.
Article
Immunology
Nadine Freitag, Dunja M. Baston-Buest, Jan-Steffen Kruessel, Udo R. Markert, Tanja N. Fehm, Alexandra P. Bielfeld
Summary: The study finds a link between endometriosis and changes in the immune cell population, providing a new approach for diagnosing endometriosis in infertility patients.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2022)
Biographical-Item
Immunology
Julia Szekeres-Bartho, Udo Markert, Thomas J. Gill
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2022)
Article
Developmental Biology
Henning Schneider, Christiane Albrecht, Mahmoud S. Ahmed, Michelle Broekhuizen, Leonie Aengenheister, Tina Buerki-Thurnherr, A. H. Jan Danserf, Sophie Gil, Stefan R. Hansson, Rick Greupink, Rohan M. Lewis, Udo R. Markert, Line Mathiesen, Nicola Powles-Glover, Christian Wadsack, Paul Brownbill
Summary: The ex vivo dual perfusion model of the human placenta cotyledon has provided valuable insights into the mechanisms of substance transfer and placental function. However, there is considerable variation in perfusion methodology between research centers, highlighting the need for standardization and harmonization to ensure consistent results and acceptance of the model by regulatory agencies.
Article
Cell & Tissue Engineering
Tina Gorsek Sparovec, Udo R. Markert, Philipp Reif, Wolfgang Schoell, Gerit Moser, Julia Feichtinger, Zala Nikita Mihalic, Julia Kargl, Caroline E. Gargett, Daniela Gold
Summary: This study investigated the effect of progesterone on the stemness of SUSD2+ eMSC isolated from non-pregnant uterine samples, and the role of these cells in decidualization during pregnancy. The results showed changes in gene expression profiles and reduced abundance of SUSD2+ eMSC during pregnancy, while their functional capacity remained intact.
STEM CELL RESEARCH
(2022)
Article
Immunology
Natalin J. Valeff, Maria S. Ventimiglia, Marcos Dibo, Udo R. Markert, Federico Jensen
Summary: B1 B cells, a distinct subpopulation of B cells, have been controversial in their role during pregnancy. This study revealed an expanded and proliferative splenic B1 B cell population in mice during pregnancy, accompanied by an immunoregulatory phenotype, suggesting their critical role in the intricate process of pregnancy tolerance.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell Biology
Milena Zarkovic, Franziska Hufsky, Udo R. Markert, Manja Marz
Summary: Non-coding RNAs have a central and regulatory role in the placenta, especially microRNAs. Syncytiotrophoblast cells act as a communication interface between the fetus and the mother through the release of extracellular vesicles containing ncRNAs. Disorders of ncRNAs in placental tissue are associated with pregnancy disorders and have potential as diagnostic markers.
Review
Immunology
Jose M. Murrieta-Coxca, Paulina Fuentes-Zacarias, Stephanie Ospina-Prieto, Udo R. Markert, Diana M. Morales-Prieto
Summary: The concept of biological identity has been a central issue in immunology. The expanded immunotolerance observed in pregnancy challenges the assumption that foreign entities should be rejected by the immune system. Microchimeric cells play a crucial role in enhancing immune tolerance and genetic fitness during pregnancy, but they may also cause abnormal immune activation. Studies have revealed a synergistic effect between microchimerism and cellular communication through extracellular vesicles (EVs), which participate in feto-maternal tolerance and antigen presentation. This review discusses the current understanding of microchimerism and the potential role of EVs in mediating allorecognition and tropism of microchimeric cells during human pregnancy.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Immunology
Michael Eikmans, Diana M. M. Morales-Prieto, Marie-Louise van der Hoorn, Udo R. R. Markert
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Endocrinology & Metabolism
Jose M. Murrieta-Coxca, Emanuel Barth, Paulina Fuentes-Zacarias, Ruby N. Gutierrez-Samudio, Tanja Groten, Alexandra Gellhaus, Angela Koeninger, Manja Marz, Udo R. Markert, Diana M. Morales-Prieto
Summary: Placenta accreta spectrum (PAS) is a group of pathological conditions characterized by abnormal attachment of the placenta to the uterus wall, and it is a major cause of maternal morbidity and mortality worldwide. In this study, altered miRNA expression, particularly in placenta-specific miRNA clusters, was identified in placenta accreta tissue compared to normal placental tissues. Dysregulated pathways associated with cell cycle regulation, inflammation, and invasion were also observed. The study provides miRNA:mRNA associations that could contribute to our understanding of PAS development.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Oncology
S. Loibl, H. A. Azim, T. Bachelot, P. Berveiller, A. Bosch, E. Cardonick, C. Denkert, M. J. Halaska, M. Hoeltzenbein, A. L. V. Johansson, C. Maggen, U. R. Markert, F. Peccatori, P. Poortmans, E. Saloustros, C. Saura, P. Schmid, E. Stamatakis, M. van den Heuvel-Eibrink, M. van Gerwen, V. Vandecaveye, G. Pentheroudakis, G. Curigliano, F. Amant
Summary: The management of breast cancer during pregnancy is a challenging area with limited evidence. In 2022, the European Society for Medical Oncology organized a virtual consensus-building process to address controversial issues and gain insights from experts in this field.
ANNALS OF ONCOLOGY
(2023)
Review
Immunology
Fernando Gomez-Chavez, Jose M. Murrieta-Goxes, Heriberto Caballero-Ortega, Diana M. Morales-Prieto, Udo R. Markert
Summary: Molecular communication between a pathogen and its host is crucial. Extracellular vesicles (EVs) act as mediators for the delivery of molecular signals. T. gondii infection during pregnancy can cause severe clinical complications. The role of EV signaling in this process remains unclear. This review summarizes the current knowledge on EV release from T. gondii and its human host cells regarding immunological consequences and placental passage.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Article
Endocrinology & Metabolism
Frantisek Staud, Xin Pan, Rona Karahoda, Xiaojing Dong, Petr Kastner, Hana Horackova, Veronika Vachalova, Udo R. Markert, Cilia Abad
Summary: This study reveals the crucial role of the placenta in regulating serotonin (5-HT) levels, including the clearance of 5-HT from the fetal circulation, its metabolism to 5-HIAA, and the subsequent excretion into the maternal circulation. This synchronized mechanism is critical for regulating 5-HT in the fetoplacental unit and can be compromised by external insults such as antidepressant drugs.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Elizabeth Soczewski, Jose Martin Murrieta-Coxca, Lucas Miranda, Paulina Fuentes-Zacarias, Ruby Gutierrez-Samudio, Esteban Grasso, Marcelo Marti, Claudia Perez Leiros, Diana Morales-Prieto, Udo R. Markert, Rosanna Ramhorst
Summary: The study reveals the role of miRNAs in regulating endoplasmic reticulum stress and unfolded protein response in decidualized cells and endometrium associated with reproductive failures. The findings highlight the potential involvement of miR-17-5p, miR-21-5p, and miR-193b-3p in the regulation of ERS/UPR pathways and inflammation during embryo implantation. The expression of these miRNAs differs in stromal cells from patients with recurrent pregnancy loss and recurrent implantation failure, suggesting alterations in the regulation of ERS/UPR pathways.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Review
Biotechnology & Applied Microbiology
Patrick Mai, Joerg Hampl, Martin Baca, Dana Brauer, Sukhdeep Singh, Frank Weise, Justyna Borowiec, Andre Schmidt, Johanna Merle Kuestner, Maren Klett, Michael Gebinoga, Insa S. Schroeder, Udo R. Markert, Felix Glahn, Berit Schumann, Diana Eckstein, Andreas Schober
Summary: Recent trends in 3D cell culturing focus not only on the microenvironment, but also on microscopic geometrical parameters for mimicking tissue models. Through technologies like micromachining and 3D printing, mimicking specific tissue-relevant substrates can be achieved.
BIOENGINEERING-BASEL
(2022)