Article
Chemistry, Multidisciplinary
Marketa Klicova, Senta Mullerova, Jachym Rosendorf, Andrea Klapstova, Radek Jirkovec, Jakub Erben, Michaela Petrzilkova, Hedvika Raabova, Dalibor Satinsky, Jana Melicherikova, Richard Palek, Vaclav Liska, Jana Horakova
Summary: This study focuses on the large-scale fabrication and testing of specific antibacterial nanofibrous materials to prevent postoperative complications associated with bacterial infection. The research demonstrates that these nanofiber materials are cytocompatible and have antibacterial properties, making them suitable for medical applications.
Article
Materials Science, Multidisciplinary
Yihan Yan, Lorenzo Travaglini, Kieran Lau, Jelena Rnjak-Kovacina, Daniel Ta, Minoo Eslami, Shaohua Yang, Antonio Lauto, David Officer, Damia Mawad
Summary: This study evaluated the effects of different sterilization methods on the physical and electronic properties of a bioelectronic patch containing polyaniline as the conjugated polymer. Ethylene oxide and gamma-irradiation at 15 and 25 kGy doses were found to be the most effective at maintaining the mechanical and electronic properties of the patch. Overall, these findings provide a pathway for the translation of organic bioelectronic devices from acute to long-term in vivo models.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Polymer Science
Christopher J. Houk, Felipe O. Beltran, Melissa A. Grunlan
Summary: The study demonstrates that sterilization with ethylene oxide has minimal impact on the material properties of polydopamine-coated self-fitting shape memory polymer scaffolds, allowing the scaffolds to maintain good bioactivity and shape memory properties.
POLYMER DEGRADATION AND STABILITY
(2021)
Review
Biotechnology & Applied Microbiology
Shuai Chen, Carmen J. Gil, Liqun Ning, Linqi Jin, Lilanni Perez, Gabriella Kabboul, Martin L. Tomov, Vahid Serpooshan
Summary: This article discusses the significance of Adhesive Tissue Engineering Scaffolds (ATESs), reviewing their key characteristics and requirements, exploring mechanisms of action for securing the scaffold onto tissue, and presenting strategies for assessing adhesive properties. Current applications and future prospects of advanced ATES systems in regenerative medicine therapies are highlighted, along with key challenges in various fields of tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Shounak Majumder, Zaiyang Long, Alexander W. Hooke, Bret T. Petersen, Ferga C. Gleeson, Marco Bruno, John DeWitt, Grace Elta, Larissa Fuji, Victoria Gomez, Laurent Palazzo, Vanessa M. Shami, Tyler Stevens, Mark D. Topazian, Maurits J. Wiersema, Lawrence J. Berglund, Barham K. Abu Dayyeh, Vinay Chandrasekhara, Prasad G. Iyer, Elizabeth Rajan, Andrew C. Storm, Kenneth K. Wang, Ryan Lennon, Joseph J. Larson, Felicity T. Enders, Jed R. Frein, Raymond A. Yates, Nicholas J. Hangiandreou, Michael J. Levy
Summary: This study compared the effects of high-level disinfection (HLD) versus HLD followed by ethylene oxide (ETO) sterilization on endoscopic ultrasound (EUS) image quality. The results showed minimal differences in image quality between the two reprocessing methods, supporting the use of ETO sterilization of EUS instruments if clinically necessary.
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yang Liu, Chuanlei Zhang, Yanhui Kong, Huiyu Liu, Cheng Chen, Wenyu Gao, Xiaowei Xi, Hui Yang, Linhong Deng
Summary: Blindness often occurs due to corneal abnormalities, and corneal transplantation is the most effective treatment method. Developing high-quality artificial corneas is crucial since there is a shortage of available donor corneas for transplantation. Collagen, the main component of natural cornea, is commonly used as a material in corneal repair. However, promoting the regeneration of corneal nerves after surgery remains a challenge. In this study, researchers developed a novel collagen-based material that showed promising results in promoting the growth of nerve cells and corneal epithelial cells. The findings suggest that this material may have potential applications in cornea tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Vera S. S. Chernonosova, Ilya E. E. Kuzmin, Inna K. K. Shundrina, Mikhail V. V. Korobeynikov, Victor M. M. Golyshev, Boris P. P. Chelobanov, Pavel P. P. Laktionov
Summary: In this study, the effects of two sterilization methods (ethylene oxide treatment and electron beam irradiation) on fibrous polyurethane-gelatin scaffolds were investigated. Ethylene oxide treatment caused significant changes in the structure and mechanical properties of the scaffolds, while electron beam irradiation did not affect the biocompatibility of the scaffolds. Therefore, electron beam irradiation is recommended as a sterilization method for polyurethane-gelatin electrospun medical devices.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Instruments & Instrumentation
Hesna Ozbek Ulkutas, Berrin Ozcelik, Inan Guler
Summary: This study aimed to design and test a new medical sterilization system using a new oxygen molecule allotrope (NOMA). Results showed that the newly designed system was 100% effective in sterilizing microbes, with advantages of lower cost, faster speed, lower temperature, residue-free, and being human and environmentally friendly compared to existing sterilization methods using NOMA.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2021)
Review
Biotechnology & Applied Microbiology
Xiaolan Li, Xiang Zhang, Ming Hao, Dongxu Wang, Ziping Jiang, Liqun Sun, Yongjian Gao, Ye Jin, Peng Lei, Yue Zhuo
Summary: This review summarizes the importance of collagen in peripheral nerve regeneration, focusing on its characteristics, role, and optimization methods. Collagen, as a biomaterial, plays a crucial role in peripheral nerve repair and can be further optimized by adjusting its structure and physical properties, as well as combining it with bioactive components.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Paola Nitti, Sanosh Kunjalukkal Padmanabhan, Serena Cortazzi, Eleonora Stanca, Luisa Siculella, Antonio Licciulli, Christian Demitri
Summary: In this study, composite structures made of hydroxyapatite scaffold impregnated with a collagen slurry were designed to mimic bone tissue structure, with the addition of magnesium and silicon ions enhancing mechanical and biological properties. An innovative freeze-drying approach was developed to create open-pore structures for tissue regeneration. The biodegradation behavior of the scaffolds, particularly HA-Mg_Coll scaffolds, showed moderate weight loss and mechanical performance reduction due to collagen dissolution, while also protecting the ceramic structure until degradation, making them suitable candidates for bone remodeling.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Xiaotang He, Wen Li, Siyang Liu, Yi Li, Yining Chen, Nianhua Dan, Weihua Dan, Meifeng Zhu
Summary: In this study, high-strength, flexible, and porous collagen-based scaffolds were successfully prepared using a novel strategy. Compared to regular collagen scaffolds, these scaffolds demonstrated superior mechanical properties and cell compatibility. Animal experiments showed that the scaffolds promoted cell infiltration, vascularization, and tissue regeneration. This study provides a new solution for tissue repair and broadens the application range in regenerative medicine.
MATERIALS TODAY BIO
(2022)
Review
Nanoscience & Nanotechnology
Shaoyan Shi, Xuehai Ou, Deliang Cheng
Summary: This comprehensive review explores the potential of nanofiber scaffolds in enhancing peripheral nerve regeneration. It covers various aspects including fabrication methods, scaffold-cell interactions, controlled release of bioactive agents, clinical translation prospects, emerging trends, and considerations for biocompatibility and safety. By elucidating the structural attributes and functional capacities of nanofiber scaffolds, it offers a prospective and effective strategy for the treatment of peripheral nerve injury.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Nanoscience & Nanotechnology
Feng Xiong, Shuo Wei, Shuyuan Wu, Wei Jiang, Biyun Li, Hongyun Xuan, Ye Xue, Huihua Yuan
Summary: In this study, a biomimetic piezoelectric and conductive nanofiber scaffold was constructed for the repair of large peripheral nerve deficits, which promoted nerve cell differentiation and nerve fiber extension. The application of the scaffold in rat sciatic nerve injury prevented autotomy and facilitated nerve regeneration and functional recovery.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Applied
Yulin Wang, Ya Li, Zhongbing Huang, Bing Yang, Ning Mu, Zhaopu Yang, Min Deng, Xiaoming Liao, Guangfu Yin, Yu Nie, Tunan Chen, Hua Ma
Summary: By constructing gene vectors and nerve scaffolds, successful localized delivery of c-Jun plasmids and upregulation of nerve growth factor expression were achieved, promoting nerve regeneration and functional recovery.
CARBOHYDRATE POLYMERS
(2022)
Article
Engineering, Environmental
Jing-Han Song, Jun-Ting Gu, Gao-Peng Dang, Zhi-Ting Li, Chen Lei, Ling Li, Zhao Mu, Franklin R. Tay, Kai Jiao, Li-na Niu
Summary: Unmodified collagen scaffolds are limited in bone tissue engineering due to their mechanical and osteoinductive properties. A DNA-crosslinked collagen scaffold (DNA-Col) is fabricated to enhance bone healing. The improved osteogenic performance of DNA-Col is attributed to its interaction with T cells, specifically regulatory T cells (Tregs). Mechanistic experiments show that DNA-Col triggers recruitment of Tregs in vivo, and depletion of Tregs reverses DNA-Col-induced bone regeneration. Further investigations reveal that DNA-Col promotes Treg differentiation through metabolic reprogramming. These findings establish the role of DNA-Col as a bioactive bone regeneration scaffold via its interaction with Tregs.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Biomedical
Ugo Masullo, Anna Cavallo, Maria Raffaella Greco, Stephan J. Reshkin, Maria Mastrodonato, Nunzia Gallo, Luca Salvatore, Tiziano Verri, Alessandro Sannino, Rosa Angela Cardone, Marta Madaghiele
Summary: This study investigated the potential of novel semi-IPN cryogels as tunable platforms for 3D cancer cell research, showing that higher collagen concentration in the cryogels allowed cancer cell lines to reproduce their characteristic growth patterns. The findings suggest the proposed cryogels have the potential to be reproducible and tunable platforms for cancer cell research.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2021)
Article
Materials Science, Characterization & Testing
Luca Salvatore, Emanuela Calo, Valentina Bonfrate, Deborah Pedone, Nunzia Gallo, Maria Lucia Natali, Alessandro Sannino, Marta Madaghiele
Summary: The optimization of collagen-based scaffolds for tissue engineering involves careful selection of crosslinking methods to achieve the desired properties without negatively impacting cellular responses. Different crosslinking treatments led to varying degrees of chemical and elastic effective crosslink density. EDC treatment promoted intramolecular crosslinks and intermolecular crosslinks, resulting in higher denaturation temperature and lower elastic effective crosslink density compared to aldehyde-based treatments. There was a correlation between denaturation temperature and chemical effective crosslink density, as well as water uptake and elastic effective crosslink density. An exponential relationship was also found between elastic effective crosslink density and in vitro half-life.
Review
Biotechnology & Applied Microbiology
Luca Salvatore, Nunzia Gallo, Maria Lucia Natali, Alberta Terzi, Alessandro Sannino, Marta Madaghiele
Summary: Collagen, a fibrous structural protein found in mammals, plays a dominant role in maintaining the integrity of various tissues and organs. Recent studies have focused on fine-tuning scaffold architecture at the microscale and nanoscale to mimic the hierarchical features of the extracellular matrix, with a particular focus on collagen patterning.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Polymer Science
Nunzia Gallo, Maria Lucia Natali, Alessandra Quarta, Antonio Gaballo, Alberta Terzi, Teresa Sibillano, Cinzia Giannini, Giuseppe Egidio De Benedetto, Paola Lunetti, Loredana Capobianco, Federica Stella Blasi, Alessandro Sicuro, Angelo Corallo, Alessandro Sannino, Luca Salvatore
Summary: Collagen is a widely used biomaterial in the health-related sectors. In recent years, there has been increasing interest in using marine organisms as a safe and abundant source for collagen extraction, particularly fish byproducts. This study focuses on the extraction and characterization of type I collagen from Tilapia skin raised in aquaponics systems, and the results confirm its potential as a biomaterial for healthcare applications.
Article
Crystallography
Alberta Terzi, Teresa Sibillano, Liberato De Caro, Davide Altamura, Nunzia Gallo, Maria Lucia Natali, Alessandro Sannino, Luca Salvatore, Federica Stella Blasi, Angelo Corallo, Cinzia Giannini
Summary: Type I collagen is widely used as a biomaterial and antiaging food supplementation. Fish collagen supplementation in the diet can increase collagen synthesis in the body. This study investigated the effect of collagen supplementation on the structure of tilapia skin and found that it had an impact at the nanoscale, but not at the submolecular scale.
Article
Biochemistry & Molecular Biology
Nunzia Gallo, Alberta Terzi, Teresa Sibillano, Cinzia Giannini, Annalia Masi, Alessandro Sicuro, Federica Stella Blasi, Angelo Corallo, Antonio Pennetta, Giuseppe Egidio De Benedetto, Francesco Montagna, Alfonso Maffezzoli, Alessandro Sannino, Luca Salvatore
Summary: In the last two decades, fish industry by-products have been used to extract type I collagen due to issues with traditional mammalian tissue extraction. This study focused on fish by-products from aquaponic plants as a source of biopolymers, aiming to create eco-friendly and profitable materials. The research evaluated the impact of age on the properties of Tilapia skin for collagen extraction, identifying significant age-related differences in thickness, collagen fiber bundle diameter, organization, orientation, thermal properties, mechanical properties, and composition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Polymer Science
Zahra Rajabimashhadi, Nunzia Gallo, Luca Salvatore, Francesca Lionetto
Summary: Fish collagen has gained attention in recent decades for its potential applications in various health-related industries. Its advantages over mammalian-based collagen, such as reduced zoonosis transmission risk and cost-effective manufacturing process, have led to the expansion of using collagen derived from fish wastes. This review summarizes the recent advances in extracting collagen from fish industry wastes for several applications and discusses the challenges in the fish-collagen market.
Review
Polymer Science
Luca Salvatore, Maria Lucia Natali, Chiara Brunetti, Alessandro Sannino, Nunzia Gallo
Summary: Soft tissues diseases have a significant impact on patients' quality of life, requiring targeted and often expensive interventions. In recent decades, there has been a growing demand for minimally invasive procedures for the treatment of complex diseases. Injectable collagen-based products have emerged as a promising approach in managing such diseases. Collagen, the most abundant protein in vertebrate connective tissues, has been widely used in healthcare and cosmetics. This review provides an update on the clinical outcomes of collagen-based injectables, highlighting their safety and effectiveness for treating various diseases.
Article
Pharmacology & Pharmacy
Nunzia Gallo, Stefano Quarta, Marika Massaro, Maria Annunziata Carluccio, Amilcare Barca, Donato Cannoletta, Luisa Siculella, Luca Salvatore, Alessandro Sannino
Summary: Vascularization is a complex and precise process, but it can be slow and inadequate for tissue repair. Biomaterial-based strategies have been developed to enhance vasculogenesis. In this study, PLGA microparticles loaded with L-lysine were developed for controlled release. The optimized formulation showed biocompatibility and supported cell proliferation and angiogenesis. L-lysine encapsulated in PLGA microparticles has potential as a cost-effective system for angiogenesis enhancement and rapid healing.
Review
Pharmacology & Pharmacy
Francesca Milano, Annalia Masi, Marta Madaghiele, Alessandro Sannino, Luca Salvatore, Nunzia Gallo
Summary: Gelatin, a versatile natural polymer, is extensively used in healthcare due to its biocompatibility, biodegradability, low-cost, and chemical group availability. In the field of biomedicine, gelatin is utilized as a biomaterial for the development of drug delivery systems (DDSs) using various synthesis techniques. This review focuses on the techniques commonly used for gelatin-based micro- or nano-sized DDSs, highlighting the potential of gelatin as a carrier for bioactive compounds and its ability to control drug release kinetics. The various techniques, such as desolvation, nanoprecipitation, coacervation, emulsion, electrospray, and spray drying, are discussed along with their effects on DDSs' properties. Additionally, preclinical and clinical studies involving gelatin-based DDSs are thoroughly examined.
Article
Polymer Science
Carmela Tania Prontera, Nunzia Gallo, Roberto Giannuzzi, Marco Pugliese, Vitantonio Primiceri, Fabrizio Mariano, Antonio Maggiore, Giuseppe Gigli, Alessandro Sannino, Luca Salvatore, Vincenzo Maiorano
Summary: Bio-based polymers have gained significant attention for their potential applications as alternatives to conventional polymers. In the field of electrochemical devices, polymers are being explored as solid-state and gel-based electrolytes to enhance device performance. This study focuses on the fabrication and characterization of collagen membranes for their potential use as a polymeric matrix for gel electrolytes. The cross-linked collagen membranes showed good stability, water absorption capability, and resistance, making them suitable candidates for electrolytes in electrochromic devices.
Review
Microbiology
Marco Friuli, Rebecca Pellegrino, Leonardo Lamanna, Paola Nitti, Marta Madaghiele, Christian Demitri
Summary: Biopolymer-based formulations enhance the effectiveness of entomopathogenic fungi as bioinsecticides, offering target specificity, eco-friendliness, and sustainability. Challenges such as production costs, stability, and shelf life need to be addressed.
Article
Biology
Rebecca Pellegrino, Stefania Villani, Daniela Spagnolo, Irene Carofalo, Nico Carrino, Matteo Calcagnile, Pietro Alifano, Marta Madaghiele, Christian Demitri, Paola Nitti
Summary: In recent years, the increasing demand for clinical swabs has raised environmental concerns due to their single use and lack of biodegradability. This study focused on producing an innovative biodegradable PVA nanofiber swab tip using electrospinning, which showed comparable uptake and release capacity to commercial foam-type swabs for protein and bacterial samples. The obtained swab is low-cost, scalable, and efficient, making it suitable for the healthcare industry.
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)
