Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 77, Issue -, Pages 1035-1043Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2017.04.046
Keywords
Collagen; Hyaluronan; In-situ formable hydrogels; Michael addition
Categories
Funding
- National Natural Science Foundation of China [31270021]
- Science and Technology Project of Guangdong Province [2014A010105031]
- Natural Science Foundation of Guangdong Province [2014A030313379]
- Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation [pdjh2016b0065]
Ask authors/readers for more resources
To mimic the natural extracellular matrix (ECM) and to facilitate tissue regeneration, in-situ formable collagen/hyaluronan composite hydrogels were prepared by a facile approach via Michael addition reaction, with maleilated collagen (Col-MA) and thiol derivatized hyaluronan (HA-SH). The hydrogels were denoted as CHG1, CHG-2, CHG-3 and CHG-4 by vinyl/free thiol molar ratio (f) of 1:1, 1:2, 1:3 and 1:4, respectively. Results showed that with decrease of the f values, the gelation time decreased from 43 s to 15 s, Young's modulus increased from 1671.65 Pa to 9105.86 Pa, and the swelling ratio increased from 1.5 to 12.7. SEM images of the air-dried samples revealed that the chemical modification process did not denature the collagen, and the interwoven collagen fibrils were indeed observed. Cell culture confirmed that the CHG facilitated the growth and proliferation of MC3T3-E1. Cells displayed spreading morphology and formed nearly aligned cell layers with the extension of culture time. Therefore, it is suggested that CHG can be used as injectable materials for tissue regeneration. (C) 2017 Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available