Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 76, Issue -, Pages 313-318Publisher
ELSEVIER
DOI: 10.1016/j.msec.2017.03.098
Keywords
PBLG/PLA nanofibers; Electrospinning; 3D culture; Melanoma
Categories
Funding
- National Natural Science Foundation of China [50603020, 50773062]
- Key Research and Development Program of Shaanxi Province [2013K09-27]
- Fundamental Research Funds for the Central Universities [XJJ2014124]
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Though much progress in utilizing tissue engineering technology to investigate tumor development in vitro has been made, the effective management of human melanoma is still a challenge in clinic due to lack of suitable 3D culture systems. In this study, we prepared a poly(-gamma-benzyl-L-glutamate)/poly(lactic acid) (PBLG/PLA) nanofiber membrane by electrospinning and demonstrated its suitability as a matrix for 3D culture of melanoma cells in vitro. The electrospun PBLG/PLA nanofiber membrane displayed a smooth and uniform fibrous morphology and had a desirable water contact angle of 79.3 +/- 0.6 degrees. The average diameter of PBLG/PLA nanofibers was 320.3 +/- 95.1 nm that was less than that (516.2 +/- 1633 nm) of pure PLA nanofibers. The addition of PBLG into PLA decreased the cold crystallization peak of PLA fibers from 93 to 75 degrees C. The in vitro biocompatibility of PBLG/PLA nanofiber membrane was evaluated with B16F10 cells using PLA nanofiber membrane as control. It was found that, compared to PLA nanofiber membrane, PBLG/PLA nanofiber membrane could better support cell viability and proliferation, as indicated by MTT assay and live-dead staining. SEM results revealed that PBLG/PLA rather than PLA nanofiber membrane promoted the generation of tumoroid-like structures. These findings clearly demonstrated that the electrospun PBLG/PLA nanofiber membrane could mimick the extracellular matrix of melanoma microenvironment and be a promising platform for 3D cell culture. (C) 2017 Elsevier B.V. All rights reserved.
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