Review
Biotechnology & Applied Microbiology
Mahender Aileni, Mallesham Bulle, Ramesh Naik Malavath, Satyamraj Thurpu, Kiranmayi Bandaram, Bhargavi Balkampeta, Meghana Marri, Vijaya Simha Reddy Singasani, E. N. Murthy
Summary: Woodfordia fruticosa is a medicinal shrub known for its various pharmacological properties. Due to its rich phyto-constituents, the plant is facing the risk of endangerment. This review provides a concise summary of the plant's constituents and biological activities, while addressing the gaps and potential areas of scientific innovation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Mohammed Abu Tayab, Kazi Ashfak Ahmed Chowdhury, Md Jabed, Syed Mohammed Tareq, A. T. M. Mostafa Kamal, Mohammad Nazmul Islam, A. M. Kafil Uddin, Mohammad Adil Hossain, Talha Bin Emran, Jesus Simal-Gandara
Summary: Extracts from Woodfordia fruticosa leaves demonstrate strong potential in antioxidative, antidiabetic, and antidepressant effects, offering potential dietary supplements for mitigating hyperglycemia, cellular toxicity, and depressive disorder.
Article
Biophysics
Karan Razdan, Shashi Kanta, Ekta Chaudhary, Seema Kumari, Deepak Kumar Rahi, Ashok Kumar Yadav, Vivek Ranjan Sinha
Summary: The study developed and characterized levofloxacin-loaded clove oil nanoscale emulgel and evaluated its therapeutic efficacy in Pseudomonas aeruginosa biofilm-infected burn wounds in mice. The results showed that the emulgel dispersed biofilms, closed wounds, promoted re-epithelialization and collagen deposition, and cleared the infection within 7 days, indicating its effectiveness in treating biofilm-infected burn wounds.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Shringika Mishra, Shruti Sonter, Manish Kumar Dwivedi, Prashant Kumar Singh
Summary: Woodfordia fruticosa is a widely used plant in traditional medicine systems with antioxidant and anti-inflammatory properties, providing potential compounds for the development of novel anti-sickling agents. Its extracts exhibited high reversal of sickled red blood cells and significant antioxidant and anti-inflammatory activities, supporting its traditional use for the treatment of anemia and blood-related complications.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Engineering, Environmental
Mingming Qin, Yanqiu Guo, Feifei Su, Xiaopeng Huang, Qiuping Qian, Yunlong Zhou, Jingye Pan
Summary: The development of functional medical dressings with mechanical designability is essential for tissue repair. However, existing liquid or gel dressings have limitations in terms of mechanical properties and antimicrobial biofilm, hindering their applications in emergency cases. In this study, a nanocomposite hydrogel dressing was developed, incorporating gold nanorods and Ca2+ into polyacrylic acid and polyvinyl alcohol, resulting in a double network structure with high toughness and excellent fatigue resistance. The hydrogel dressings exhibited good biocompatibility, self-healing function, and efficient elimination of bacterial biofilm. Moreover, the dressing was found to promote wound healing in vivo.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Hanif Haidari, Richard Bright, Sanjay Garg, Krasimir Vasilev, Allison J. Cowin, Zlatko Kopecki
Summary: The development of highly potent ultrasmall AgNPs combined with a biocompatible hydrogel has shown promising results in eliminating mature biofilm infections in vivo and promoting wound healing. The treatment exhibited superior antibacterial efficacy and improved wound closure, re-epithelization, and collagen deposition compared to conventional silver-based treatments.
Article
Engineering, Environmental
Ki-Seok Han, Anbazhagan Sathiyaseelan, Kandasamy Saravanakumar, Myeong-Hyeon Wang
Summary: The study successfully extracted high-quality bovine bone-derived hydroxyapatite (BB-HAP) through acid/alkali treatment and calcination process, demonstrating its biocompatibility for use in wound dressing and implantable materials.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Nanoscience & Nanotechnology
Dengyan Wu, Dong Wei, Maotao Du, Song Ming, Qian Ding, Ranjing Tan
Summary: In this study, novel chitosan and poly (D,L-lactide-co-glycolide) nanoparticles loaded with benzalkonium bromide (BZK) were developed for wound healing after MRSA infection. These nanoparticles exhibited significant antibacterial activity, anti-biofilm properties, and the ability to damage bacterial cell walls, indicating their potential in promoting skin wound healing. These findings suggest a new avenue for nanomedicine development in wound treatment.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Walker D. D. Short, Oluyinka O. O. Olutoye, Benjamin W. W. Padon, Umang M. M. Parikh, Daniel Colchado, Hima Vangapandu, Shayan Shams, Taiyun Chi, Jangwook P. P. Jung, Swathi Balaji
Summary: Impaired wound healing is a significant financial and medical burden. Disruptions in ECM deposition, structure, and composition lead to impaired healing in diseased states, such as in diabetes. Valid measures include lack of bacterial contamination, good tissue perfusion, and reduced mechanical injury and strain. This review discusses bioengineering advances in detecting biologic and physiologic factors, visualizing and modeling the ECM, and efficiently evaluating wound data. It focuses on bioelectronics and biologic interfaces for real-time sensing and actuation, advanced imaging techniques, and computational modeling. These advancements have the potential to improve healing outcomes and guide decision-making in wound care.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Medicine, Research & Experimental
Aakansha Giri Goswami, Somprakas Basu, Tuhina Banerjee, Vijay Kumar Shukla
Summary: The biofilm, a community of microorganisms protected by a self-produced matrix, plays a vital role in infection and antimicrobial resistance. The formation, growth, and resistance mechanism of biofilm are still not fully understood. Although there have been studies on anti-biofilm and antimicrobial therapeutics, the lack of a clear clinical standard calls for the translation of laboratory research into novel bedside strategies. Biofilm is responsible for faulty wound healing and chronic wounds. Understanding its interaction with wounds and developing reproducible anti-biofilm measures in a clinical setting is the current challenge.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Chemistry, Medicinal
Yuan-E Lee, Takeshi Kodama, Nwet Nwet Win, Dae-Won Ki, Nhat Nam Hoang, Chin Piow Wong, Khine Zar Wynn Lae, Hla Ngwe, Tohru Dairi, Hiroyuki Morita
Summary: Phytochemical investigation of Woodfordia fruticosa flowers led to the identification of flavonoids with anti-SmltD activity, with compound 1 showing the most potent inhibition. Further study revealed compound 1 exerted competitive inhibition with respect to ATP, providing a foundation for the development of novel SmltD inhibitors.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Review
Biochemistry & Molecular Biology
Sourbh Suren Garg, Rupal Dubey, Sandeep Sharma, Ashish Vyas, Jeena Gupta
Summary: Chronic wounds in diabetes mellitus patients are difficult to heal due to high glucose levels, oxidative stress, and biofilm-associated microbial infection. Conventional antibiotic therapies are ineffective in clinical settings as antibiotics cannot penetrate the microbial biofilm matrix. Therefore, finding safer alternatives to reduce chronic wound infection is urgently needed. Using biological-macromolecule based nano-delivery systems to inhibit biofilm formation offers advantages such as higher drug loading efficiency, sustained drug release, enhanced drug stability, and improved bioavailability. This review discusses the pathogenesis, microbial biofilm formation, and immune response of chronic wounds, as well as the potential of macromolecule-based nanoparticles as wound healing therapies to reduce mortality associated with chronic wound infections.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Dermatology
Fergus Watson, Rui Chen, Steven L. Percival
Summary: This study aimed to evaluate the antibacterial properties of commercially available wound dressings, with different material composition and antibacterial agents, against various in vitro microbial and biofilm models. The results showed that controlled-release iodine foam and silver-impregnated carboxymethylcellulose (CMC) wound dressings had better biofilm management properties compared to methylene blue and gentian violet-containing foam dressings. The controlled-release iodine foam and silver-impregnated CMC materials effectively prevented the dissemination of Pseudomonas aeruginosa.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Agnieszka Najda, Aarti Bains, Prince Chawla, Anil Kumar, Sebastian Balant, Magdalena Walasek-Janusz, Dariusz Wach, Ravinder Kaushik
Summary: The study aimed to evaluate the antimicrobial and anti-inflammatory activity of ethanolic extract of Woodfordia fruticosa flowers, which was rich in phenolic, flavonoid, and ascorbic acid. The extract showed significant antimicrobial efficacy against pathogenic bacteria and fungi, as well as higher anti-inflammatory activity. This suggests that the ethanolic extract of Woodfordia fruticosa flowers could be a valuable source of phenols for manufacturing value-added food products.
Article
Engineering, Environmental
Junyao Song, Haoyu Chen, Yaqian Lv, Wanqun Yang, Fenglang Zhang, Tianyi Wang, Danping Liu, Yingshan Qu, Lei Han, Jun Fu, Xiaoying Kong
Summary: This study developed a hybrid single atom nanozyme material composed of Zn single-atom catalysts and copper peroxide, which exhibited superior antibacterial properties and therapeutic effects for the treatment of biofilm-infected wounds.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Tara Chand Yadav, Naresh Kumar, Utkarsh Raj, Nidhi Goel, Pritish Kumar Vardawaj, Ramasare Prasad, Vikas Pruthi
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2020)
Correction
Engineering, Biomedical
Shweta Singh, Priya Vashisth, Alok Shrivastav, Naresh Bhatnagar
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Priya Vashisth, Neelakshi Kar, Deepak Gupta, Jayesh R. Bellare
Article
Chemistry, Multidisciplinary
Manoj Kushwaha, Arem Qayum, Shreyans K. Jain, Jasvinder Singh, Amit Kumar Srivastava, Shubham Srivastava, Nisha Sharma, Vidushi Abrol, Ruchi Malik, Shashank K. Singh, Ram A. Vishwakarma, Sundeep Jaglan
Summary: Seven cytochalasins were isolated from a fungal extract, with 19,20-epoxycytochalasin C showing higher cytotoxic potential compared to its oxidized metabolite. Mass spectrometry analysis revealed the oxidation of 19,20-epoxycytochalasin C to a less cytotoxic metabolite, indicating a potential target for cancer treatment.
Article
Immunology
Amit Kumar Srivastava, Tara Chand Yadav, Harvinder Kour Khera, Purusottam Mishra, Navdeep Raghuwanshi, Vikas Pruthi, Ramasare Prasad
Summary: Psoriasis is an inflammatory skin disease with complex pathogenesis and multiple etiological factors. The discovery of IL-23/Th17 axis has revealed the mechanism of accelerated inflammation in psoriasis, leading to insights into disease pathogenesis and revolutionizing therapeutic approaches. However, a unifying mechanism for understanding the disease pathogenesis is still lacking.
JOURNAL OF AUTOIMMUNITY
(2021)
Article
Medicine, Research & Experimental
Isha Khurana, Prince Allawadhi, Amit Khurana, Amit Kumar Srivastava, Umashanker Navik, Anil Kumar Banothu, Kala Kumar Bharani
Summary: Bilirubin has various beneficial properties, including anti-inflammatory, antioxidant, and antiviral activities, and may act as a metabolic hormone with positive associations to several diseases. However, challenges in therapeutic delivery exist due to solubility issues. Nanotechnology offers unique advantages for improved delivery and potential use in combating COVID-19.
MEDICAL HYPOTHESES
(2021)
Article
Biochemistry & Molecular Biology
Arjun Singh Raghuwanshi, Ankit Kumar, Navdeep Raghuwanshi, Shravan Kumar Singh, Avinash Kumar Singh, Umanath Tripathi, Swarnendu Kaviraj, Sanjay Singh
Summary: This study successfully expressed full-length PfRH5 in E. coli, optimized the purification and refolding process to obtain high purity antigen. Furthermore, PfRH5 formulated with adjuvant GLA-SE in mice showed high antibody titers, indicating its potential as a malaria vaccine.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Pharmacology & Pharmacy
Purusottam Mishra, Payal Gupta, Amit Kumar Srivastava, Krishna Mohan Poluri, Ramasare Prasad
Summary: This study utilized EPNF nanofibers to eradicate Candida biofilms, which possess highly hydrophilic surface facilitating rapid drug release, inhibiting approximately 70% of biofilms. Encapsulation of eucalyptol prolonged its antifungal activity.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Tara Chand Yadav, Payal Gupta, Saakshi Saini, Shanid Mohiyuddin, Vikas Pruthi, Ramasare Prasad
Summary: In this study, tyrosol-functionalized chitosan gold nanoparticles (Chi-TY-AuNPs) were synthesized and shown to have high antifungal and antibiofilm activities against Candida albicans and C. glabrata. They were able to reduce protein and DNA content in the fungal biofilms, decrease ergosterol content, and downregulate the expression of certain genes. Overall, Chi-TY-AuNPs demonstrate potential as a therapeutic solution for Candida biofilm-related chronic infections.
Article
Biochemistry & Molecular Biology
Amit Kumar Srivastava, Shubham Srivastava, Viney Kumar, Souvik Ghosh, Siddharth Yadav, Ruchi Malik, Partha Roy, Ramasare Prasad
Summary: This study aimed to identify potential NF-kB inhibitors using virtual screening and molecular docking, and evaluate their therapeutic efficacy using cell-based assays. Myricetin and hesperidin were found to scavenge intracellular ROS and inhibit NF-kB activation. Molecular dynamics simulations showed that myricetin and hesperidin formed stable complexes with the target protein and induced a closed conformation of NF-kB. This study provides important insights into the binding mechanism of NF-kB inhibitors and their potential as antipsoriatic drug candidates.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Nanoscience & Nanotechnology
Purusottam Mishra, Payal Gupta, Rajnish Srivastava, Amit Kumar Srivastava, Krishna Mohan Poluri, Ramasare Prasad
Summary: In this study, p-cymene-loaded gellan gum (GA)/poly(vinyl alcohol) (PVA) nanofibers with strong antibiofilm activity against Candida albicans and Candida glabrata were prepared using electrospinning. In vitro biocompatibility assay showed no cytotoxicity to NIH3T3 cell lines. In vivo, the nanofibers promoted faster healing of skin lesions compared to traditional clotrimazole gel without scarring. These findings suggest that p-cymene-loaded GA/PVA nanofibers are an effective biomaterial for cutaneous tissue regeneration.
ACS APPLIED BIO MATERIALS
(2023)
Article
Materials Science, Biomaterials
Sonu Singh, Sunil Kumar Yadav, Vijay Kumar Meena, Priya Vashisth, Dinesh Kalyanasundaram
Summary: The ability of bone to regenerate itself through mechanobiological responses is crucial for effective bone tissue regeneration. Permeability and fluid flow-induced wall shear stress are crucial parameters for cell bioactivities and bone tissue regeneration. The 3D-printed open cell porous scaffold with Neovius architecture shows comparable blood flow permeability and structural strength to human bone.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Materials Science, Multidisciplinary
Alok Srivastava, Ramya Ahuja, Pooja Bhati, Shweta Singh, Pankaj Chauhan, Priya Vashisth, Avinash Kumar, Naresh Bhatnagar
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)
