Article
Fisheries
Areli Silva-Becerril, Adrian Quintero-Martinez, Alejandra Hernandez-Santoyo
Summary: A galacturonic acid-specific lectin, AcL, was purified from the sea hare Aplysia californica using galactose-agarose affinity chromatography. AcL has a molecular mass of 27.5 kDa and shows high affinity for D-galacturonic acid and low affinity for galactosides.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Roopashri N. Arekal, J. Savitha, Ashwini Kumar, B. S. Gunashree, M. S. Divyashree
Summary: A freshwater green microalgal strain was isolated and a lectin with strong hemagglutination activity was identified. The lectin was purified using an affinity chromatographic technique and characterized. The study revealed that the lectin has similar characteristics to a hemagglutinin, indicating its potential for various biological purposes.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Romulo F. Carneiro, Evelyn S. Aguiar, Valdenice F. Santos, Ana L. E. Santos, Maria H. C. Santos, Renato R. Roma, Romerio R. S. Silva, Maria L. M. B. Leal, Livia T. Silva, Bruno A. M. Rocha, Claudio G. L. Silva, Celso S. Nagano, Alexandre H. Sampaio, Racquel O. S. Souza, Claudener S. Teixeira
Summary: The research focused on the structure-function study of plant lectins, particularly in elucidating their mechanisms in cell models. The results showcased the amino acid sequencing, three-dimensional structure prediction, and inhibitory effect of P. pendula lectin on Leishmania infantum promastigote development. The study confirmed the leishmanicidal potential of PpeL, suggesting its utility as a new strategy for antileishmanial drug development.
PROCESS BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ting Wang, Meiyun Jiang, Shuo Tang, Chenhuan Lai, Caoxing Huang, Yimin Fan, Qiang Yong
Summary: A novel biorefining strategy was established to prepare di-GalA and tri-GalA from commercial pectin, with hydrothermal pretreatment and enzymatic hydrolysis enhancing the yield and selectivity of oligogalacturonic acids.
PROCESS BIOCHEMISTRY
(2021)
Article
Parasitology
Lachlan Coff, Jodie L. Abrahams, Simon Collett, Cecilia Power, Barbara F. Nowak, Daniel Kolarich, Nathan J. Bott, Paul A. Ramsland
Summary: Infections by blood flukes are a significant health issue for the aquaculture industry in Japan and Australia. This study characterized the glycans of Cardicola forsteri using histochemistry and mass spectrometry techniques. The findings provide insights into the glycomic profile of a marine platyhelminth and have implications for research on other trematodes.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Hong Shu, Lei Zhang, Yiwei Chen, Yijie Guo, Limin Li, Fanghua Chen, Zhao Cao, Guoquan Yan, Chunlai Lu, Chao Liu, Shu Zhang
Summary: This study identified stronger O-glycosylation of Haptoglobin in patients with hepatocellular carcinoma compared to liver cirrhosis, and quantified the differences using mass spectrometry, providing a comprehensive understanding of the glycosylation of Hp in liver diseases.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Analytical
Benjamin J. Burris, Abraham K. Badu-Tawiah
Summary: Enzyme-catalyzed hydrolysis of lipids was monitored directly in immiscible microdroplet environments using contained-electrospray mass spectrometry. Different lipid types were utilized to identify possible mechanisms for lipase reactivity in aerosols, showing enhancement factors favoring the droplet experiment.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Franz-Georg Hanisch, Cem Aydogan, Horst Schroten
Summary: Norovirus infections are a major cause of human gastroenteritis worldwide, with epidemic outbreaks causing numerous deaths annually. This study investigates the potential of polyfucoses to block the binding of the most prevalent norovirus strain to human and animal gastrointestinal mucins, providing insight into novel structural aspects of fucoidan and derived oligosaccharides. This research is a first step towards the development of a prophylactic food additive from algal species to attenuate the spread of noroviruses.
Article
Chemistry, Physical
Alessandro Boverio, Hugo L. van Beek, Simone Savino, Adeline Ranoux, Wouter J. J. Huijgen, Harry W. C. Raaijmakers, Marco W. Fraaije, Nikola Loncar
Summary: This study reports the discovery of a new flavin-containing carbohydrate oxidase URAO(Cs3) from Citrus sinensis, which showed high thermostability and selectivity for uronic acids. The enzyme was highly effective in selectively oxidizing D-galacturonic acid, while leaving other carbohydrates untouched. These findings suggest that plant proteomes can serve as an interesting source for new biocatalysts.
Article
Food Science & Technology
Minghui Yan, Liwen Luo, Dandan Li, Zhenmin Liu, Ran Wei, Jia Yi, Liang Qiao, Chunping You
Summary: We developed an efficient strategy to assess the risk of biofilm formation by psychrotrophic Pseudomonas in raw milk using MALDI-TOF MS. By collecting molecular mass fingerprints of whole cells and combining multivariate statistical analysis with machine learning, we established a classification model for the biofilm-forming ability of psychrotrophs. The results showed that different biofilm-forming capabilities of Pseudomonas can be well distinguished by the MALDI-TOF MS molecular mass fingerprints. The established classification model demonstrated 100% accuracy in identifying biofilm-forming risk.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ke Wang, Xiao-Ping Peng, Dan Feng, Chuan Tian, Wen-Long Wan, Hong-Xiang Lou, Gang Li
Summary: In this study, a variety of GAs compounds were isolated from a fungus and their spectroscopic data were reported. Compound 1 showed a promoting effect on the growth of Arabidopsis thaliana seedlings.
Article
Agriculture, Multidisciplinary
Pengpu Wang, Guoyu Sun, Pei Lu, Yuchen Zhu, Xiaosong Hu, Fang Chen
Summary: This study focuses on the role of galacturonic acid in the formation of acrylamide. The results show that the addition of galacturonic acid can accelerate the formation of acrylamide, and the formation of acrylamide is closely related to pH value and temperature. Moreover, some intermediates were identified, contributing to the formation of acrylamide in fruit products.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Multidisciplinary Sciences
Nankun Qin, Ming Qin, Wenjun Shi, Lingbo Kong, Liting Wang, Guang Xu, Yuying Guo, Jiayu Zhang, Qun Ma
Summary: Metabolomics research is of great significance in finding biomarkers and exploring the pathogenesis of Hyperuricemia (HUA). The combination of untargeted and targeted metabolomics can provide more reliable results.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Allan J. Alla, Keith J. Stine
Summary: Continuous efforts have been made in developing monolithic materials for the separation, enrichment, and digestion of glycoproteins. This review discusses and summarizes recent work in this field, focusing on strategies utilizing monolithic materials for glycoprotein analysis. These studies contribute to a better understanding of the structure-function relationship of glycans in glycoproteins.
Article
Immunology
Kun Wang, Zetao Sun, Fei Zhu, Yunping Xu, Feng Zhou
Summary: The HLA system plays a crucial role in the human immune system and its typing methods have been extensively studied for organ transplantation. In this study, we developed a novel HLA typing technology based on nucleic acid mass spectrometry. Our method accurately typed HLA by measuring the molecular weights of HLAMSTTs with SNPs. The results were validated by PCR-SBT, and the method proved to be rapid, efficient, accurate, and applicable to homozygous and heterozygous samples.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Applied
Ariana Azevedo Vasconcelos, Idia Nara de Sousa Veras, Mayron Alves de Vasconcelos, Alexandre Lopes Andrade, Helcio Silva Dos Santos, Paulo Nogueira Bandeira, Elnatan Bezerra de Souza, Maria Rose Jane Ribeiro Albuquerque, Edson Holanda Teixeira
Summary: This study investigated the antibacterial and antibiofilm activities of essential oils extracted from Ruellia asperula and Ruellia paniculata against oral streptococci. The results showed that the essential oils exhibited inhibitory effects on planktonic growth and biofilm formation, suggesting their potential as alternatives for bacterial growth control and biofilm formation prevention of oral streptococci.
NATURAL PRODUCT RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Larissa Maximiano Resende, Erica de Oliveira Mello, Mariana Carvalho de Lima Aguieiras, Celso Shiniti Nagano, Renata Pinheiro Chaves, Gabriel Bonan Taveira, Marciele Souza da Silva, Andre de Oliveira Carvalho, Rosana Rodrigues, Valdirene Moreira Gomes
Summary: This study investigated the antimicrobial peptides present in Capsicum chinense fruits and evaluated their enzymatic and antifungal activities. The results showed that these peptides exhibited inhibitory effects on various fungi and demonstrated enzyme inhibition potential. These findings suggest the biotechnological potential of these antimicrobial peptides in plant disease control.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biotechnology & Applied Microbiology
Marilucia C. Ribeiro, Rodrigo S. Gebara, Gabriel B. Taveira, Andre de O. Carvalho, Rosana Rodrigues, Erica O. Mello, Celso S. Nagano, Renata P. Chaves, Valdirene M. Gomes
Summary: This study purified and evaluated the antifungal peptides present in the fruits of Capsicum chinense Jacq. The results showed that the D2 fraction of these fruits significantly inhibited the growth of Candida albicans and C. tropicalis yeasts. Mass spectrometry analysis revealed that the 6 kDa peptide band of the HI9 fraction showed similarity with antimicrobial peptides belonging to the plant defensin family.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biochemistry & Molecular Biology
Aluska Kelly A. Nunes, Ellen Araujo Malveira, Alexandre Lopes Andrade, Wildson Max Barbosa da Silva, Selene Maia de Morais, Helcio Silva dos Santos, Cynthia Cavalcanti de Albuquerque, Edson Holanda Teixeira, Diego Nathan do Nascimento Souza, Mayron Alves de Vasconcelos
Summary: The chemical composition of Croton blanchetianus Baill leaves' essential oil (EO) was analyzed and found to have antimicrobial and antibiofilm activities against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli strains. The EO showed synergistic effects with ampicillin and tetracycline. It also significantly inhibited biofilm formation and reduced the number of viable cells in biofilms. EO may have potential as a natural product for preventing bacterial biofilm infections.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Microbiology
Edson Holanda Teixeira, Alexandre Lopes Andrade, Rafael Pereira, Livia Pontes Farias, Gabrieli Sobral Monteiro, Marcia Machado Marinho, Emmanuel Silva Marinho, Helcio Silva Santos, Mayron Alves de Vasconcelos
Summary: The present study aimed to evaluate the antibacterial, antibiofilm, and synergistic effect of triterpene CLF1 against Staphylococcus aureus and Staphylococcus epidermidis strains. The results showed that CLF1 exhibited bacteriostatic and bactericidal activity against all strains tested, and synergistic effect with ampicillin was observed only for S. epidermidis strains. Additionally, CLF1 significantly inhibited biofilm formation and disrupted preformed biofilm of all strains.
CURRENT MICROBIOLOGY
(2023)
Article
Microbiology
Anna L. Pereira, Mayron A. Vasconcelos, Alexandre L. Andrade, Israel M. Martins, Alda K. M. Holanda, Ana C. S. Gondim, Dayana P. S. Penha, Katherine L. Bruno, Francisco O. N. Silva, Edson H. Teixeira
Summary: Health care-associated infections (HAIs) are a major problem that causes high morbidity, mortality, and financial burden. This study evaluated the effectiveness of copper-based metallic compounds against planktonic cells and biofilm formation of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli. The compounds showed bacteriostatic and bactericidal activity and significantly reduced biofilm biomass, colony-forming units, and metabolic activity. However, they did not exhibit DNA degradation activity but showed a capacity to cleave DNA upon addition of glutathione, leading to reactive oxygen species formation.
CURRENT MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Renata Pinheiro Chaves, Ana Katia Barbosa dos Santos, Alexandre Lopes Andrade, Aryane de Azevedo Pinheiro, Juliana Meneses de Sena Silva, Francisca Manuela Santos da Silva, Jucilene Pereira de Sousa, Ito Liberato Barroso Neto, Eduardo Henrique Salviano Bezerra, Jade Oliveira Abreu, Fatima Cristiane Teles de Carvalho, Oscarina Viana de Sousa, Bruno Lopes de Sousa, Bruno Anderson Matias da Rocha, Andre Luis Coelho Silva, Luiz Gonzaga do Nascimento Neto, Mayron Alves de Vasconcelos, Edson Holanda Teixeira, Romulo Farias Carneiror, Alexandre Holanda Sampaio, Celso Shiniti Nagano
Summary: The SfL-1 isoform from Solieria filiformis displayed similar hemagglutinating activity and inhibition to native SfL. Circular dichroism analysis revealed fi-strands structures for both lectins. The three-dimensional structure of rSfL-1 was determined, showing two fi-barrel domains formed by five antiparallel fi chains. Both SfL and rSfL-1 exhibited agglutination activity against Escherichia coli and Staphylococcus aureus, with rSfL-1 showing a greater reduction in colony-forming units.
Article
Biochemistry & Molecular Biology
Jessica de Assis Duarte, Jose Eduardo de Oliveira Neto, Renato Cezar Farias Torres, Andressa Rocha de Oliveira Sousa, Alexandre Lopes Andrade, Renata Pinheiro Chaves, Mayron Alves de Vasconcelos, Claudener Souza Teixeira, Edson Holanda Teixeira, Celso Shiniti Nagano, Alexandre Holanda Sampaio, Romulo Farias Carneiro
Summary: This study structurally characterized and evaluated the antibacterial potential of a lectin isolated from the marine sponge Aplysina lactuta (ALL). The lectin showed affinity for specific glycoproteins and exhibited antibacterial effects when combined with certain antibiotics, suggesting its potential for the development of new antibacterial drugs.
Article
Microbiology
Mayron Alves Vasconcelos, Bruno Rocha da Silva, Alexandre Lopes Andrade, Aryane de Azevedo Pinheiro, Francisco Flavio Vasconcelos Evaristo, Francisco Vassiliepe Sousa Arruda, Esteban Nicolas Lorenzon, Eduardo Maffud Cilli, Edson Holanda Teixeira
Summary: The study aimed to evaluate the antimicrobial and antibiofilm activities of the native peptide KR-12 and its derivative, the synthetic peptide [W7]KR12-KAEK, against Enterococcus faecalis strains. The results showed that [W7]KR12-KAEK exhibited potential antimicrobial activity against planktonic cells and could inhibit biofilm formation and disrupt mature biofilms. These findings suggest that [W7]KR12-KAEK may be a potential molecule for auxiliary antimicrobial therapies against oral infections.
CURRENT MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Francisco R. N. Andrade, Pedro A. S. Tabosa, Renato C. F. Torres, Romulo F. Carneiro, Mayron A. Vasconcelos, Alexandre L. Andrade, Elielton Nascimento, Ulisses Pinheiro, Edson H. Teixeira, Celso S. Nagano, Alexandre H. Sampaio
Summary: A lectin (HiL) with galactose specificity was isolated from marine sponge Haliclona (Reniera) implexiformis using affinity chromatography. Potent inhibitors of HiL included glycoproteins porcine stomach mucin (PSM) and bovine stomach mucin (BSM). HiL showed maximal hemagglutinating activity at pH 5.0-9.0 and remained active up to 60 degrees C. It consists of a dimer with identical subunits linked by disulfide bonds and has a significant impact on Staphylococcus biofilms.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2023)
Article
Multidisciplinary Sciences
Alexandra S. De Almeida, Dayara N. M. Mendonca, Romulo F. Carneiro, Ulisses Pinheiro, Elielton Francisco Do Nascimento, Alexandre l. Andrade, Mayron A. De Vasconcelos, Edson H. Teixeira, Celso S. Nagano, Alexandre H. Sampaio
Summary: A new lectin IsL was isolated from the marine sponge Ircinia strobilina. It exhibited stable agglutination activity and had a specific secondary structure. IsL is calcium-dependent and able to inhibit biofilm formation of Staphylococcus aureus and Staphylococcus epidermidis.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2023)