BSA-AuNPs@Tb-AMP metal-organic frameworks for ratiometric fluorescence detection of DPA and Hg2+

An easy and effective strategy for synthesizing a ratiometric fluorescent nanosensor has been demonstrated in this work. Novel fluorescent BSA-AuNPs@Tb-AMP (BSA, bovine serum albumin; AMP, adenosine 5-monophosphate; AuNPs, Au nanoparticles) metal-organic framework (MOF) nanostructures were synthesized by encapsulating BSA-AuNPs into Tb-AMP MOFs for the detection of 2,6-pyridinedicarboxylic acid (DPA) and Hg2+. DPA could strongly co-ordinate with Tb3+ to replace water molecules from the Tb3+ center and accordingly enhanced the fluorescence of Tb-AMP MOFs. The fluorescence of BSA-AuNPs at 405nm remained constant. While the fluorescence of BSA-AuNPs at 635nm was quenched after Hg2+ was added, the fluorescence of Tb-AMP MOFs remained constant. Accordingly, a ratiometric fluorescence nanosensor was constructed for detection of DPA and Hg2+. The ratiometric nanosensor exhibited good selectivity to DPA over other substances. The F-545/F-405 linearly increased with increase of DPA concentration in the range of 50nM to 10M with a detection limit as low as 17.4nM. F-635/F-405 increased linearly with increase of Hg2+ concentration ranging from 50nM to 1M with a detection limit as low as 20.9nM. Additionally, the nanosensor could be successfully applied for the determination of DPA and Hg2+ in running water.