Article
Gastroenterology & Hepatology
Guillaume Rossignol, Xavier Muller, Valerie Hervieu, Sophie Collardeau-Frachon, Antoine Breton, Natacha Boulanger, Mickael Lesurtel, Remi Dubois, Kayvan Mohkam, Jean-Yves Mabrut
Summary: This single-center pilot study presents feasibility and safety data for transplantation of partial liver grafts undergoing ex situ split during HOPE, suggesting improved preservation compared with static ex situ splitting.
LIVER TRANSPLANTATION
(2022)
Article
Multidisciplinary Sciences
Ngee-Soon Lau, Mark Ly, Claude Dennis, Andrew Jacques, Marti Cabanes-Creus, Shamus Toomath, Joanna Huang, Nicole Mestrovic, Paul Yousif, Sumon Chanda, Chuanmin Wang, Leszek Lisowski, Ken Liu, James G. Kench, Geoffrey McCaughan, Michael Crawford, Carlo Pulitano
Summary: Long-term machine perfusion of human livers outside the body is an emerging field with tremendous potential for the assessment, recovery, and modification of organs prior to transplantation. Here, the authors report the long-term ex situ perfusion of human livers and demonstrate the ability to split and perfuse these organs using a standardised protocol.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Pierre-Alain Clavien, Philipp Dutkowski, Matteo Mueller, Dilmurodjon Eshmuminov, Lucia Bautista Borrego, Achim Weber, Beat Muellhaupt, Richard X. Sousa Da Silva, Brian R. Burg, Philipp Rudolf von Rohr, Martin J. Schuler, Dustin Becker, Max Hefti, Mark W. Tibbitt
Summary: A human liver has been successfully transplanted using ex situ normothermic machine perfusion, which extends the time window for evaluating the viability of donor organs and transforms the urgency of the surgery to an elective procedure.
NATURE BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
M. Magbagbeola, Z. L. Rai, K. Doyle, L. Lindenroth, G. Dwyer, A. Gander, A. Stilli, B. R. Davidson, D. Stoyanov
Summary: This paper presents a low-cost organ perfusion machine that is modular and versatile, allowing for the addition of specific sensors for different research applications. The machine was assessed for perfusion efficacy, functionality, and viability using various measurements and assays. The results show that the system is capable of successfully perfusing porcine livers for up to three hours, maintaining their viability and functionality.
INTERNATIONAL JOURNAL OF COMPUTER ASSISTED RADIOLOGY AND SURGERY
(2023)
Article
Immunology
Paulo N. Martins, Michael D. Rizzari, Davide Ghinolfi, Ina Jochmans, Magdy Attia, Rajiv Jalan, Peter J. Friend
Summary: In liver machine perfusion trials, proper trial design and data interpretation are crucial. Recommendations include publicly sharing protocols before trials, ensuring sufficient statistical power, and carefully considering randomization timing for primary outcomes.
Article
Surgery
Rohit Gaurav, Andrew J. Butler, Vasilis Kosmoliaptsis, Lisa Mumford, Corrina Fear, Lisa Swift, Arturs Fedotovs, Sara Upponi, Samir Khwaja, James Richards, Michael Allison, Christopher J. E. Watson
Summary: NRP and NMP were associated with better early liver function compared to SCS, whereas NRP was associated with superior preservation of the biliary system.
Article
Medicine, General & Internal
Mohammed Al-Sebayel, Yasser M. El-Sheikh, Falah H. Al-Mohanna, Saleh Al Abbad, Yaser H. Al Nemry, Ahmed Al-Jammali, Norah K. Al-Zeer, Yazeed M. Alsebayel, Hamad M. Al-Bahli
Summary: The study established a successful normothermic oxygenated ex-vivo liver perfusion model using sheep liver, achieving continuous perfusion for up to 9 hours with stable hemodynamic parameters and bile production. Further exploration on this sheep model appears to be promising.
SAUDI MEDICAL JOURNAL
(2021)
Review
Medicine, General & Internal
Fabio Melandro, Riccardo De Carlis, Francesco Torri, Andrea Lauterio, Paolo De Simone, Luciano De Carlis, Davide Ghinolfi
Summary: This review summarizes the current literature on viability criteria during normothermic and hypothermic machine perfusion, and discusses the future steps and evolution of these technologies.
MEDICINA-LITHUANIA
(2022)
Article
Surgery
Joseph A. Attard, Daniel-Clement Osei-Bordom, Yuri Boteon, Lorraine Wallace, Vincenzo Ronca, Gary Reynolds, M. T. P. R. Perera, Ye Htun Oo, Hynek Mergental, Darius F. Mirza, Simon C. Afford
Summary: The development of a split liver ex situ machine perfusion protocol provides comparative controls for investigating machine perfusion techniques and therapeutic interventions. Results show comparability between right and left lobes when simultaneously subjected to normothermic machine perfusion, indicating the potential for pre-clinical investigations.
FRONTIERS IN SURGERY
(2021)
Review
Medicine, General & Internal
Claire Goumard, Celia Turco, Mehdi Sakka, Lynda Aoudjehane, Philippe Lesnik, Eric Savier, Filomena Conti, Olivier Scatton
Summary: The ongoing organ shortage has led transplant teams to develop ex-situ machine perfusion as an alternative source for liver grafts. Normothermic machine perfusion has shown promise in defatting livers, with successful results on human organs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Immunology
Sanne J. J. Langmuur, Jorik H. Amesz, Kevin M. Veen, Ad J. J. C. Bogers, Olivier C. Manintveld, Yannick J. H. J. Taverne
Summary: This article presents a meta-analysis on the use of the Organ Care System (OCS) for heart transplantation. The analysis found that using OCS for heart perfusion showed similar outcomes to static cold storage in terms of survival and secondary outcomes.
Review
Surgery
Nicholas Gilbo, Joris Blondeel, Jacques Pirenne, Renato Romagnoli, Giovanni Camussi, Diethard Monbaliu
Summary: Machine perfusion (MP) has enabled transplant physicians to evaluate and select suitable grafts for transplantation. However, the discard of damaged organs still leads to an imbalance in organ supply. Strategies to repair and regenerate organs before transplantation are urgently needed, and MP has the potential to meet this need. Current administration of mesenchymal stromal cells (MSC) during MP has limitations due to inefficient delivery, but the use of MSC secretome and extracellular vesicles therapy during MP seems to be more efficient.
TRANSPLANT INTERNATIONAL
(2023)
Article
Cardiac & Cardiovascular Systems
Lu Wang, Guy A. MacGowan, Simi Ali, John H. Dark
Summary: Heart transplantation remains the most effective treatment for advanced heart failure patients, but the gap between donor hearts and transplant recipients continues to widen due to the increasing prevalence of heart failure worldwide. Ex situ machine perfusion has been crucial in recent heart transplant activities, enabling the use of donation after circulatory death hearts and extending the interval between procurement and implantation. However, there is currently no consensus on optimal preservation temperature, perfusate composition, and perfusion parameters.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2021)
Review
Transplantation
Hanns Lembach Jahnsen, Hynek Mergental, M. Thamara P. R. Perera, Darius F. Mirza
Summary: Key studies in liver preservation over the last 3 years have shown advancements in normothermic and hypothermic machine perfusion techniques, with potential to improve organ quality in transplantation. Ongoing research focuses on evaluating efficacy of interventions and optimal indications for different perfusion strategies based on graft type and clinical scenario.
CURRENT OPINION IN ORGAN TRANSPLANTATION
(2021)
Review
Surgery
Belle Liew, David Nasralla, Satheesh Iype, Joerg-Matthias Pollok, Brian Davidson, Dimitri A. Raptis
Summary: Machine perfusion, especially hypothermic machine perfusion, showed advantages in reducing early allograft dysfunction and improving recipient outcomes in liver transplant surgeries. Normothermic machine perfusion was associated with reduced graft injury and major complication risks. Both techniques have the potential to lead to cost savings.
BRITISH JOURNAL OF SURGERY
(2021)
Review
Medicine, General & Internal
L. Leonie van Leeuwen, Henri G. D. Leuvenink, Peter Olinga, Mitchel J. R. Ruigrok
Summary: Efforts have been made to address the demand for donor kidneys by using kidneys from donation after circulatory death donors. However, these kidneys are more prone to develop interstitial fibrosis and tubular atrophy due to severe ischemia and reperfusion injury. Current treatments, including machine perfusion, are unable to effectively ameliorate interstitial fibrosis and tubular atrophy. In this review, the authors propose a new therapeutic approach that supplements machine perfusion solutions with anti-fibrotic compounds. Several compounds, including LY2109761, galunisertib, imatinib, nintedanib, and butaprost, have shown promising anti-fibrotic effects and are considered excellent candidates for further ex vivo machine perfusion studies.
FRONTIERS IN MEDICINE
(2022)
Article
Transplantation
Aukje Brat, Leonie H. Venema, Bas W. J. Bens, Remy Stieglis, Joris J. van der Heijden, Constantino Fondevila, Oleg N. Reznik, Benoit Barrou, Michiel E. Erasmus, Henri G. D. Leuvenink
Summary: Implementation of uDCD programs should not only be based on the number of potential donors calculated from resuscitation databases, but also consider geographical regions and demographic factors. Large urban regions have the potential for more donors, but it is important to note that the actual numbers per hospital may be low.
TRANSPLANTATION DIRECT
(2022)
Article
Immunology
Eliano Bonaccorsi-Riani, Andrew R. Gillooly, Samuele Iesari, Isabel M. A. Bruggenwirth, Chantal M. Ferguson, Mina Komuta, Daela Xhema, Aurelie Daumerie, Louis Maistriaux, Henri Leuvenink, Jerzy Kupiec-Weglinski, Robert J. Porte, Anastasia Khvorova, David R. Cave, Pierre Gianello, Paulo N. Martins
Summary: The study showed that inhibition of the FAS gene through siRNA therapy can reduce the severity of ischemia-reperfusion injury after liver transplantation in a static cold storage protocol. However, using siRNA therapy in combination with a HOPE perfusion model presents challenges and requires further research with better designed compounds and appropriate dosages to prove its effectiveness.
Article
Engineering, Biomedical
Zhang Long Zhang, Niels Moeslund, Michiel Andy Hu, Roland Hoffmann, Leonie Harmina Venema, Caroline Van De Wauwer, Wim Timens, Toshihiro Okamoto, Erik Alfons Maria Verschuuren, Henri Gerrit Derk Leuvenink, Hans Eiskjaer, Michiel Elardus Erasmus
Summary: Sheep lungs acquired from an abattoir can be perfused ex vivo under similar conditions as lab animal lungs, with similar results in terms of oxygenation and ATP restoration. Furthermore, costs can be significantly reduced by using this abattoir model. By increasing accessibility and lowering costs for lung perfusion experiments, more results can be achieved in the field of lung diseases.
Article
Engineering, Biomedical
Rianne Schutter, Otis C. van Varsseveld, Veerle A. Lantinga, Merel B. F. Pool, Tim H. Hamelink, Jan Hendrik Potze, Henri G. D. Leuvenink, Christoffer Laustsen, Ronald J. H. Borra, Cyril Moers
Summary: This study introduces a novel technique of using MRI during ex vivo normothermic machine perfusion for renal grafts, allowing detailed real-time visualization and assessment of structural abnormalities, regional flow distribution, renal metabolism, local oxygen availability, and distribution of ex vivo administered cellular therapy. This platform provides an advanced tool for pre-transplant organ assessment and renal physiology and metabolism research.
Article
Transplantation
Rianne Schutter, Willemijn A. L. Vrijlandt, Gelske M. Weima, Robert A. Pol, Jan-Stephan F. Sanders, Meindert J. Crop, Henri G. D. Leuvenink, Cyril Moers
Summary: Understanding the reasons for kidney discard is crucial for optimal utilization of deceased donor kidneys. A retrospective cohort study in the Netherlands analyzed the kidney utilization of 3856 kidneys and found that the traditional definition underestimated organ discard, with a non-utilization rate of 24.4%.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2023)
Article
Immunology
Baran Ogurlu, Carolina C. Pamplona, Isa M. Van Tricht, Tim L. Hamelink, Veerle A. Lantinga, Henri G. D. Leuvenink, Cyril Moers, Merel B. F. Pool
Summary: This study suggests that gradual rewarming during controlled oxygenated rewarming (COR) before normothermic machine perfusion (NMP) can provide the best protective effect against rewarming injury. Specifically, a rewarming rate of 1.10%/min during COR-120 results in superior immediate tubular function and energetic recovery during NMP.
Article
Urology & Nephrology
Tim J. Knobbe, Daan Kremer, Rianne M. Douwes, Michele F. Eisenga, Antonio W. Gomes-Neto, Coby Annema, J. Casper Swarte, Frank Klont, Gerjan Navis, Stefan P. Berger, Stephan J. L. Bakker
Summary: Prior studies have shown that the use of proton pump inhibitors (PPIs) can negatively impact gut microbiota and the absorption of micronutrients, such as iron and magnesium. This study aims to investigate the association between PPI use and fatigue and health-related quality of life (HRQoL) in kidney transplant recipients.
AMERICAN JOURNAL OF KIDNEY DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Pien Rawee, Daan Kremer, Ilja M. M. Nolte, Henri G. D. Leuvenink, Daan J. J. Touw, Martin H. H. De Borst, Stephan J. L. Bakker, Mark R. R. Hanudel, Michele F. F. Eisenga
Summary: Heavy metal exposure has been linked to an increased risk of chronic kidney disease (CKD) and its progression. Iron deficiency, another common problem in CKD patients, is hypothesized to play a crucial role in the damaging effects of heavy metal exposure. Iron receptors upregulated by iron deficiency may lead to increased uptake of heavy metals in the intestine, while iron deficiency may also contribute to the retention of heavy metals in the kidney. Iron supplementation might be a strategy to combat these detrimental processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
G. J. Julia Huisman, Nora A. Spraakman, Jeroen V. Koomen, A. Marrit Talsma, Robert A. Pol, Stefan P. Berger, Henri G. D. Leuvenink, Michel M. R. F. Struys, Gertrude J. Nieuwenhuijs-Moeke
Summary: Early non-invasive detection and prediction of graft function after kidney transplantation is important. The study analyzed four urinary biomarkers and found that KIM-1 and NAG at early timepoints after transplantation were significant predictors for eGFR, while NGAL and NAG at day 1 after transplantation were negative predictors. Including these biomarkers improved the prediction models for graft outcome. Factors such as timing of measurement and transplantation factors need to be considered when using urinary biomarkers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hanno Maassen, Leonie H. Venema, Marc G. Weiss, Tobias M. Huijink, H. Sijbrand Hofker, Anna K. Keller, Tom E. Mollnes, Marco Eijken, Soren E. Pischke, Bente Jespersen, Harry van Goor, Henri G. D. Leuvenink
Summary: Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aimed to improve the flush-out and decrease ischemic injury by adding hydrogen sulphide (H2S) to the flush medium. The addition of H2S during flush out and static cold storage resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers.
Review
Biochemistry & Molecular Biology
Carolina Campos Pamplona, Cyril Moers, Henri G. D. Leuvenink, L. Leonie van Leeuwen
Summary: In recent years, significant efforts have been made to improve the preservation of (sub)optimal donor organs through ex vivo perfusion. However, there is a lack of biomarkers to assess renal viability. Therefore, it is crucial to explore new techniques for pre-transplant evaluation of organ quality to ensure successful transplantation outcomes in the long term.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Physiology
Raul Devia Rodriguez, Eline Huizing, Cagdas unlu, Frank F. J. Simonis, Reinoud P. H. Bokkers, Jean-Paul P. M. de Vries, Richte C. L. Schuurmann, Dalibor Nakladal, Hendrik Buikema, Jan-Luuk Hillebrands, Henri G. D. Leuvenink
Summary: This study developed and validated an ex vivo flow model to test the effects of endovascular therapy on vessel wall morphology, endothelial denudation, and endothelial-dependent vasomotor function. The results showed a significant reduction in endothelial cell denudation, vasoconstriction, and endothelium-dependent relaxation after endovascular intervention.
JOURNAL OF VASCULAR RESEARCH
(2023)
Article
Multidisciplinary Sciences
Lisa B. B. Westenberg, Marcel Zorgdrager, Tim D. A. Swaab, Marco van Londen, Stephan J. L. Bakker, Henri G. D. Leuvenink, Alain R. R. Viddeleer, Robert A. A. Pol
Summary: Low muscle mass and myosteatosis are associated with poor clinical outcomes, and computed tomography (CT) imaging is a reliable method for objectively assessing muscle mass and quality. This study established age-, sex-, and body mass index (BMI)-specific reference values for skeletal muscle parameters and found a significant correlation between muscle mass and urinary creatinine excretion. These results provide valuable information for clinical decision making.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
J. Casper Swarte, Tim J. Knobbe, Johannes R. Bjork, Ranko Gacesa, Lianne M. Nieuwenhuis, Shuyan Zhang, Arnau Vich Vila, Daan Kremer, Rianne M. Douwes, Adrian Post, Evelien E. Quint, Robert A. Pol, Bernadien H. Jansen, Martin H. de Borst, Vincent E. de Meijer, Hans Blokzijl, Stefan P. Berger, Eleonora A. M. Festen, Alexandra Zhernakova, Jingyuan Fu, Hermie J. M. Harmsen, Stephan J. L. Bakker, Rinse K. Weersma
Summary: This study investigates the association between the gut microbiome and Health-Related Quality of Life (HRQoL) in kidney transplant recipients. The results show that multiple bacterial species are associated with lower HRQoL in this population, and physical and mental HRQoL explain a significant part of the variance in the gut microbiome. Additionally, metabolic and neuroactive pathways are also associated with lower HRQoL, suggesting potential modifiable factors to improve HRQoL.
NATURE COMMUNICATIONS
(2023)