Article
Environmental Sciences
Hwang-Ju Jeon, Jungeun Park, Sung-Eun Lee
Summary: Chlorpyrifos-methyl (CPM), a thiophosphate insecticide, is metabolized to 3,5,6-tri-chloro-2-pyridinol (TCP) in the environment. CPM and TCP were evaluated for their toxicities on zebrafish embryos, with LC50 values of over 2000 μg/L and 612.5 μg/L, respectively. TCP exhibited greater toxicity than CPM, causing pericardial and yolk sac edema, abnormal heart development, and decreased heartbeats in zebrafish embryos.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Pharmacology & Pharmacy
Haina Gao, Jinwang Li, Guoping Zhao, Yixuan Li
Summary: TCP is an important biomarker that inhibits secretion of sex hormones. Research suggests that CPF can affect the paracrine function of Sertoli cells by reducing AR expression, while both TCP and CPF decrease AR expression. Additionally, DEP has no effect on Sertoli cells.
Article
Ecology
Gustavo Echeverri-Jaramillo, Beatriz Jaramillo-Colorado, Consuelo Sabater-Marco, Maria-Angeles Castillo-Lopez
Summary: Chlorpyrifos is widely used as an organophosphate insecticide with neurotoxic effects, cytotoxicity, and endocrine disruption. Results from in vitro assays show that the metabolite 3,5,6-trichloro-2-pyridinol is more toxic than Chlorpyrifos, and both exhibit estrogenic activity less potent than 17 beta-estradiol. Yeast strains isolated from marine sediments containing pesticide residues could potentially serve as new toxicity test models.
Article
Environmental Sciences
Shivani Uniyal, Rajesh Kumar Sharma, Vishnu Kondakal
Summary: The study found that the bacterial consortium ECO-M can effectively degrade CP in the Himalayan mountains, presenting a degradation pathway. The experimental results demonstrate that under optimized factors, the consortium can achieve a degradation rate of up to 94.3% in contaminated soil.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Pharmacology & Pharmacy
Mao Huang, Mingshan Zou, Shuangshuang Mao, Wenqi Xu, Yu Hong, Haiyan Wang, Fei Gui, Lei Yang, Fuzhi Lian, Rong Chen
Summary: The metabolite of organophosphate pesticide chlorpyrifos, 3,5,6-Trichloro-2-pyridinol, has toxic effects on the peripheral auditory system. The study investigated the damage caused by this metabolite to cochlear cells and an auditory cell line, revealing oxidative stress, cell apoptosis, and DNA damage as underlying mechanisms. The findings provide important insight into the risk assessment of exposure to this toxic substance.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2023)
Article
Environmental Sciences
Hui-Yun Liu, Zhuo Guan, Jian-Hua Cheng, Xiang-Yu Tang, Qing-Song Xian
Summary: Chlorpyrifos, a widely used insecticide in agriculture, was found to rapidly dissipate in floodwater following application, with higher temperatures during the daytime contributing to increased dissipation rates. Early rain events caused a peak release of chlorpyrifos and its degradation product, 3,5,6-trichloro-2-pyridinol (TCP), into soil pore water. Future studies should pay attention to the runoff and leaching risks of weakly sorbing TCP.
SOIL & SEDIMENT CONTAMINATION
(2022)
Article
Engineering, Environmental
Ranu Yadav, Puja Khare
Summary: This study evaluated the dissipation of chlorpyrifos (CP) and its hydrolytic metabolite 3,5,6-trichloro-2-pyridinol (TCP) in soil under different vegetation. Results showed that the presence of plants significantly reduced the half-life of CP and CP had an inhibitory effect on soil enzymes involved in carbon, nitrogen, phosphorus, and sulfur mineralization. C. flexuosus cultivars demonstrated a higher dissipation rate of CP.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Sonal Yadav, Mohd Aamir Khan, Ranju Sharma, Anushree Malik, Satyawati Sharma
Summary: The study demonstrates the potential of Dyadobacter jiangsuensis in degrading chlorpyrifos, with a formulated strain achieving enhanced degradation efficiency. The research opens the possibility of utilizing bacterial formulations to remediate chlorpyrifos polluted soils.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biotechnology & Applied Microbiology
Himanshu Mali, Chandni Shah, Darshan H. Patel, Ujjval Trivedi, R. B. Subramanian
Summary: Organophosphates are hazardous pesticides that contaminate soil and water sources. A new strain of Arthrobacter bacteria has been discovered, which has the ability to rapidly degrade organophosphates such as chlorpyrifos. It shows high efficiency and tolerance in degrading these toxic compounds.
BIORESOURCES AND BIOPROCESSING
(2022)
Article
Green & Sustainable Science & Technology
Shanggui Sun, Dongxing Ren, Wenjuan Lei, Xiangyang Zhou
Summary: This study investigated the adsorption and release of 3,5,6-Trichloro-2-pyridinol (TCP) in soil using biochar as an amendment. The results showed that higher biochar content led to increased adsorption ability and reduced release of TCP. The optimal adsorption and release occurred at a biochar content of 5%.
Article
Chemistry, Inorganic & Nuclear
Shiwang Hu, Dan Xu, Jiashen Zhou, Shangnan Du, Ke Zhao, Haixia Li, Rong Chen, Dan Zhou
Summary: A novel NiS nanocatalyst with high catalytic activity and electroactivity was successfully synthesized by using a sulfidation method, showing great potential in reducing 4-nitrophenol and methyl orange. This work demonstrates the use of simple coordination complex as sacrificial templates for nanoparticle synthesis, which could have wide applications in catalysis.
INORGANIC CHEMISTRY COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Lixia Wang, Rongfei Ye, Panming Jian, Jiangyong Liu
Summary: Metal-free catalysts, such as pumpkin-derived N-doped porous carbon, have shown great potential in catalytic applications due to their cost-competitiveness, efficiency, and recyclability. The enhanced performance of the N-doped carbon catalyst in reducing 2-methyl-4-nitrophenol is attributed to strong substrate adsorption and activation induced by electronic modulation through N-doping. This study paves the way for utilizing pumpkin in high-value applications and further developing metal-free strategies in catalysis.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sijie Fan, Xinyu Zhao, Wenqian Xie, Xiaoying Yang, Wenyang Yu, Zeqi Tang, Yuan Chen, Zhengrong Yuan, Yingying Han, Xia Sheng, Haolin Zhang, Qiang Weng
Summary: Exposure to PNMC affects follicle development and ovarian function by interfering with epigenetic modification and disrupting the expression of clock genes.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Pharmacology & Pharmacy
Suqin Hu, Huazhen Liu, Shuang Tao, Yanan Wang, Shiqi Wang, Yun Liao, Pengyuan Zheng, Pingchang Yang
Summary: Dysfunction of immune regulation plays a crucial role in the pathogenesis of many immune disorders. Environmental pollution, such as the environmental pollutant 3-methyl-4-nitrophenol (MNP), can contribute to immune deregulation. This study found that MNP compromised immune regulatory functions in the intestine, reducing the frequency of regulatory T cells and promoting the conversion of Tregs to Th17 cells. MNP induced the expression of IL-6 in Tregs through the activation of estrogen receptor. IL-6, in synergy with TGF-beta, further converted Tregs to Th17 cells. The concomitant exposure of MNP and specific antigen induced an allergic response in mice. Modulating the ER-STAT3-IL-6 signal pathway attenuated the allergic response. In summary, MNP acts as an immunoadjuvant for developing allergic reactions, and the estrogen receptor plays a key role in this process.
Article
Environmental Sciences
Zepeng Sun, Yue Ni, Yuandong Wu, Wenqing Yue, Ge Zhang, Jianmei Bai
Summary: In this study, a highly efficient electrocatalytic oxidation anode was developed using a TiO2 nanotube arrays (TiO2-NTA) intermediate layer and a La-PbO2 catalytic layer for the degradation of model pollutants. Electrochemical analysis showed that the electrode exhibited higher oxidation peak current, electrochemical active surface area, and oxygen evolution potential. Furthermore, the electrochemical degradation of the pollutants followed a pseudo-first-order kinetics model, with removal efficiencies of 70.2% and 72.8%.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Medicine, Legal
Mizuho Nosaka, Yuko Ishida, Yumi Kuninaka, Akira Taruya, Akihiko Kimura, Emi Shimada, Hiroki Yamamoto, Tomomi Michiue, Fukumi Furukawa, Toshikazu Kondo
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2020)
Article
Medicine, Legal
Akiko Ishigami, Satoshi Hata, Yuko Ishida, Mizuho Nosaka, Yumi Kuninaka, Hiroki Yamamoto, Emi Shimada, Yumiko Hashizume, Tatsunori Takayasu, Akihiko Kimura, Fukumi Furukawa, Toshikazu Kondo
Summary: This is a rare case of complete decapitation suicide using a self-constructed guillotine, with the body severely burned. The cause of death was determined to be cervical transection, with suspicion that the severed head was accidentally burned by a fire from a car accident.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2021)
Article
Medicine, Legal
Mizuho Nosaka, Yuko Ishida, Yumi Kuninaka, Akiko Ishigami, Akira Taruya, Emi Shimada, Yumiko Hashizume, Hiroki Yamamoto, Akihiko Kimura, Fukumi Furukawa, Toshikazu Kondo
Summary: The study used a mouse deep vein thrombosis model to investigate the intrathrombotic expression of AQP-1 and AQP-3, finding that the changes in AQP-1 and AQP-3 within the thrombus at different time points could help determine the age of the thrombus.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2021)
Article
Medicine, Legal
Jumpei Matsuki, Akiko Ishigami, Motonari Tanaka, Satoshi Hata, Yuko Ishida, Mizuho Nosaka, Yumi Kuninaka, Hiroki Yamamoto, Emi Shimada, Yumiko Hashizume, Tatsunori Takayasu, Akihiko Kimura, Fukumi Furukawa, Toshikazu Kondo
Summary: A 73-year-old female died of streptococcal toxic shock syndrome (STSS) after receiving intra-articular hyaluronic acid injections, with autopsy revealing necrosis and bacterial infection at the injection sites. Research suggests that the injection process may have been improper, leading to bacterial entry.
Article
Medicine, Legal
Akiko Ishigami, Yumi Iwahashi, Yuko Ishida, Mizuho Nosaka, Yumi Kuninaka, Hiroki Yamamoto, Yumiko Hashizume, Toshikazu Kondo
Summary: This report presents a fatal case of femoral artery injury caused by high-pressure water, resulting in hemorrhagic shock. The victim, a young male construction worker, was found with severe bleeding in the left femoral region and ultimately died due to the disruption of the femoral artery and vein.
JOURNAL OF FORENSIC AND LEGAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Akiko Ishigami, Masayuki Kashiwagi, Yuko Ishida, Kenji Hara, Mizuho Nosaka, Aya Matsusue, Hiroki Yamamoto, Brian Waters, Toshikazu Kondo, Shin-ichi Kubo
Summary: Japan, a mountainous country surrounded by the sea, has the second-highest rate of drowning deaths in the world. Pleural effusion is a major finding in autopsy cases of drowning, with a significant impact on diagnosis. Research suggests that the PE ratio may be more effective for diagnosing drowning cases.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Yumi Kuninaka, Yuko Ishida, Mizuho Nosaka, Akiko Ishigami, Akira Taruya, Emi Shimada, Akihiko Kimura, Hiroki Yamamoto, Mitsunori Ozaki, Fukumi Furukawa, Toshikazu Kondo
Summary: HO-1 showed significantly higher expression in the hearts of AIHD cases compared to non-AIHD cases, indicating its potential as a valuable marker for diagnosing AIHD in forensic pathology. Myoglobin pallor regions and leukocyte infiltration did not show significant differences between the two groups.
SCIENTIFIC REPORTS
(2021)
Review
Medicine, General & Internal
Yuko Ishida, Mizuho Nosaka, Toshikazu Kondo
Summary: Appropriate technology and target cells and molecules are crucial factors for determining wound vitality or wound age in forensic practice. In this study, bone marrow-derived cells were investigated as promising markers and their potential usefulness in estimating wound age was examined. The evaluation of the appearance combination of these cells and other markers may provide a more objective and accurate estimation of wound age.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, General & Internal
Sadahiro Iwabuchi, Kyohei Miyamoto, Mayuko Hatai, Yurina Mikasa, Masahiro Katsuda, Shin-ichi Murata, Toshikazu Kondo, Hiroki Yamaue, Shinichi Hashimoto
Summary: By analyzing lung samples from a COVID-19 patient, this study provides insights into the pathophysiology of the disease at a specific time point and the localization of the virus and inflammatory responses in different lung lobes.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, General & Internal
Siying Zhang, Yuko Ishida, Akiko Ishigami, Mizuho Nosaka, Yumi Kuninaka, Satoshi Hata, Hiroki Yamamoto, Yumiko Hashizume, Jumpei Matsuki, Haruki Yasuda, Akihiko Kimura, Fukumi Furukawa, Toshikazu Kondo
Summary: In this study, the expression of ubiquitin in neck skin samples was examined using immunohistochemistry, revealing a decreased expression in the compression regions, possibly due to impaired conversion and synthesis caused by compression. This finding suggests that immunohistochemical examination of ubiquitin expression in neck skin can serve as a valuable marker for diagnosing antemortem compression.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, Legal
R. Cecchi, M. Sassani, G. Agugiaro, E. Caroppo, P. De Lellis, A. Sannella, M. Mazza, T. Ikeda, T. Kondo, V. Masotti
Summary: Femicide, the extreme form of violence against women, is often underestimated and lacks data in many countries. This article proposes a shared definition based on a comprehensive review of legislation and advocates for a standardized forensic approach to identify femicide cases and standardize research.
Article
Multidisciplinary Sciences
Yumi Kuninaka, Yuko Ishida, Akiko Ishigami, Mizuho Nosaka, Jumpei Matsuki, Haruki Yasuda, Ayumi Kofuna, Akihiko Kimura, Fukumi Furukawa, Toshikazu Kondo
Summary: This study investigated the gene expression dynamics of M1 and M2 macrophage markers during skin wound healing in mice. The results showed that M1 macrophage markers peaked at 1-3 days after injury, while M2 macrophage markers peaked at 6 days after injury. The analysis of human wound specimens revealed that a M1/M2 ratio greater than 3.0 could indicate a wound age of 1-5 days.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Yuko Ishida, Siying Zhang, Yumi Kuninaka, Akiko Ishigami, Mizuho Nosaka, Isui Harie, Akihiko Kimura, Naofumi Mukaida, Toshikazu Kondo
Summary: Intense neutrophil infiltration is a characteristic of acetaminophen-induced acute liver injury. In this study, the efficacy of a specific neutrophil elastase inhibitor, sivelestat, was investigated in mice with acetaminophen-induced liver injury. Treatment with sivelestat resulted in improved survival, reduced liver damage, decreased NOS-II expression, and diminished neutrophil infiltration. These findings suggest a new clinical application for sivelestat in the treatment of acetaminophen-induced liver failure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Yuko Ishida, Yumi Kuninaka, Naofumi Mukaida, Toshikazu Kondo
Summary: Fibrosis and structural remodeling of lung tissue can seriously impact lung function and have fatal consequences. The causes of pulmonary fibrosis (PF) vary, including allergens, chemicals, radiation, and environmental particles. The cause of idiopathic PF (IPF), one of the most common forms, is still unknown. The murine bleomycin (BLM) model has been extensively studied to understand the mechanisms of PF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Yosuke Katayama, Akira Taruya, Manabu Kashiwagi, Yuichi Ozaki, Yasutsugu Shiono, Takashi Tanimoto, Takanori Yoshikawa, Toshikazu Kondo, Atsushi Tanaka
Summary: This study investigates the association between in vivo cholesterol crystals (CCs) detected by optical coherence tomography (OCT) and the no-reflow phenomenon after successful percutaneous coronary intervention (PCI) in patients with acute ST-segment elevation myocardial infarction (STEMI). The results show that the combination of the number of CCs and lipid arc can predict the no-reflow phenomenon after PCI with a high accuracy of 86%.
IJC HEART & VASCULATURE
(2022)
Article
Medicine, Legal
Shigeki Tsuneya, Makoto Nakajima, Maiko Yoshida, Yumi Hoshioka, Fumiko Chiba, Go Inokuchi, Suguru Torimitsu, Hirotaro Iwase
Summary: This case report highlights the detection of diatoms in a non-drowned human who had aspirated mud. Careful analysis of diatoms in various samples can help differentiate true drowning from false-positives in non-drowning cases.
Article
Medicine, Legal
Jessika Camatti, Ivan Galliani, Antonello Cirnelli, Rossana Cecchi
Summary: This article presents a case study on the evaluation of criminal responsibility of a murderer. The multidisciplinary approach was used to investigate and analyze the case, and through psychological tests and interviews, experts concluded that the accused cannot be exempted from criminal responsibility for the homicide.
