Article
Biochemistry & Molecular Biology
Pilar Eraso, Maria J. Mazon, Victoria Jimenez, Patricia Pizarro-Garcia, Eva P. Cuevas, Jara Majuelos-Melguizo, Jesus Morillo-Bernal, Amparo Cano, Francisco Portillo
Summary: Lysyl oxidase-like 2 (LOXL2) is known as an extracellular enzyme involved in extracellular matrix remodeling, but recent research reveals its intracellular functions in gene transcription, development, differentiation, proliferation, migration, cell adhesion, angiogenesis, and cancer progression. LOXL2 is also capable of inducing epithelial-to-mesenchymal transition (EMT), the first step in metastasis. Through the analysis of LOXL2's nuclear interactome, this study identifies its interaction with numerous RNA-binding proteins (RBPs) involved in RNA metabolism. Gene expression analysis and computational identification of RBPs' targets further suggest six candidates as LOXL2 substrates, warranting future mechanistic analysis. These findings propose novel functions of LOXL2 in tumorigenesis.
Review
Biochemistry & Molecular Biology
Yan Chen, Yuan Zhang, Xiangyu Zhou
Summary: Nucleoporins play important roles not only in nuclear pore complexes but also in the base of cilia, with non-classical functions that are linked to human diseases. Elucidating these non-classical roles of nucleoporins will enhance our understanding of ciliopathy etiology.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Cell Biology
Zhonghao Mao, Bingyu Wang, Teng Zhang, Baoxia Cui
Summary: This article discusses the biogenesis of m6A, atypical expressions of m6A regulators, and their functions in cervical cancer. The modification of m6A in non-coding RNA is also elucidated. In the context of precision medicine, the application of m6A in clinical diagnosis and treatment of cervical cancer is summarized. Additionally, new perspectives on detection methods, immune regulation, and nano-drug development are presented.
CELL DEATH & DISEASE
(2023)
Review
Plant Sciences
M. Arif Ashraf
Summary: This review article focuses on the localization and functional role of nuclear envelope proteins during cell division, with a specific emphasis on the evolution of these proteins in land plant species. While our current knowledge about the localization and function of nuclear envelope proteins during mitosis is limited, more experiments are needed to determine the functional roles of different nuclear envelope proteins in cell division.
Article
Biochemistry & Molecular Biology
Yi Zhu, Gerald W. Hart
Summary: O-GlcNAc is a dynamic post-translational modification that affects protein functions. In this study, a dual RNA-aptamer-based approach was developed to selectively increase O-GlcNAcylation of (3-catenin without affecting other OGT substrates. The increased O-GlcNAcylation of (3-catenin stabilized the protein, altered its interactions with (3-TrCP and EZH2, and resulted in significant changes in the transcriptome. The use of riboswitches or an inducible expression system coupled with aptamers allowed for inducible regulation of protein-specific O-GlcNAcylation.
Review
Medical Laboratory Technology
Wenhui Liu, Min Zeng, Nian Fu
Summary: The nuclear receptor superfamily, a family of ligand-activated transcription factors, plays a key role in cell metabolism and human diseases. They undergo various post-translational modifications, influencing diverse physiological activities. Nuclear receptors are crucial in lipid metabolism, inflammation, while SUMOylation nuclear receptors regulate function and impact cell metabolism.
CLINICA CHIMICA ACTA
(2021)
Review
Immunology
Farooq Rashid, Zhixun Xie, Muhammad Suleman, Abdullah Shah, Suliman Khan, Sisi Luo
Summary: In this review, the mechanisms by which SARS-CoV-2 evades the host immune system through various proteins were discussed. Certain viral proteins have been well studied, while the mechanisms of immune evasion by other proteins remain unclear. The perspectives of SARS-CoV-2 proteins were also elaborated.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Nagarjuna Reddy Pamudurti, Ines Lucia Patop, Aishwarya Krishnamoorthy, Osnat Bartok, Roni Maya, Noam Lerner, Reut Ashwall-Fluss, Jacob Vinay Vikas Konakondla, Tsevi Beatus, Sebastian Kadener
Summary: This study reveals that muscleblind-derived circular RNAs (circRNAs) regulate the MBL protein through cis- and trans-acting mechanisms. It demonstrates the unique functions of circMbl in trans and uncovers the reciprocal regulation between MBL-C and circMbl.
Article
Multidisciplinary Sciences
Ivan Koludarov, Tobias Senoner, Timothy N. W. Jackson, Daniel Dashevsky, Michael Heinzinger, Steven D. Aird, Burkhard Rost
Summary: This study investigates the evolution of 3-finger toxins in snake venom, highlighting a crucial shift from membrane-bound to secretory proteins. This transformation, involving changes in gene expression and the loss of a membrane-anchoring domain, played a significant role in the development of their venomous function.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Bastien Proust, Martina Radic, Nikolina Skrobot Vidacek, Cecile Cottet, Stephane Attia, Frederic Lamarche, Lucija Ackar, Vlatka Godinic Mikulcic, Malgorzata Tokarska-Schlattner, Helena Cetkovic, Uwe Schlattner, Maja Herak Bosnar
Summary: NME6 is predominantly expressed as a 186 amino acid protein, with a less abundant isoform. It remains monomeric and does not form oligomers with NME1-NME4, resulting in the inability to catalyze phosphotransfer. NME6 localizes to mitochondria and is associated with dysfunctional oxidative phosphorylation, linking it to RCC1L and mitoribosomes in regulating mitochondrial translation.
CELL AND BIOSCIENCE
(2021)
Review
Oncology
Erik Knutsen, Adrian L. Harris, Maria Perander
Summary: NEAT1 is a highly abundant nuclear architectural long non-coding RNA, playing important roles in cellular stress and developmental processes. Abnormal expression of NEAT1 in many cancers may be associated with therapy resistance and poor clinical outcome.
BRITISH JOURNAL OF CANCER
(2022)
Review
Plant Sciences
Feng Xiong, Edwin P. Groot, Yan Zhang, Sha Li
Summary: In eukaryotic cells, nuclear activities are isolated by the nuclear envelope, leading to the evolution of a highly conserved nuclear transport machinery, with the importin beta family playing a crucial role. This review summarizes the biological functions of importin beta family members in plants, including development, reproduction, stress responses, and immunity, highlighting new molecular functions such as protein turnover, miRNA regulation, and signaling. It provides a comprehensive view of this versatile protein family in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Plant Sciences
Yihao Li, Xin Zhang, Yi Zhang, Haiyun Ren
Summary: This review focuses on the organization and dynamics of actin filaments and microtubule network in guard cells during stomatal movement. The role of cytoskeletal-associated proteins in cytoskeletal rearrangements during stomatal movement is also discussed. The potential mechanisms of stomatal movement in relation to the cytoskeleton are explored, providing a foundation for further research in this field.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Samikshan Dutta, Navatha Shree Polavaram, Ridwan Islam, Sreyashi Bhattacharya, Sanika Bodas, Thomas Mayr, Sohini Roy, Sophie Alvarez Y. Albala, Marieta Toma, Anza Darehshouri, Angelika Borkowetz, Stefanie Conrad, Susanne Fuessel, Manfred Wirth, Gustavo B. Baretton, Lorenz C. Hofbauer, Paramita Ghosh, Kenneth J. Pienta, David L. Klinkebiel, Surinder K. Batra, Michael H. Muders, Kaustubh Datta
Summary: This study identified a novel AR transcriptional complex involving NRP2, which promotes the expression of CRPC-specific genes. Inhibition of NRP2 was found to enhance the sensitivity of CRPC cells to anti-AR therapies.
Article
Cell Biology
Zhinan Ren, Jun Li, Shanwen Zhao, Qi Qiao, Runguang Li
Summary: The study revealed that MCM8 is significantly overexpressed in osteosarcoma tissues, influencing tumor grade, cell proliferation, apoptosis, and migration. Silencing CTGF enhances the regulatory effects of MCM8 knockdown and alleviates the impact of MCM8 overexpression on osteosarcoma development.
CELL DEATH & DISEASE
(2021)
Article
Oncology
M. Kathryn Leonard, Marian Novak, Devin Snyder, Grace Snow, Nidhi Pamidimukkala, Joseph R. McCorkle, Xiuwei H. Yang, David M. Kaetzel
EXPERIMENTAL CELL RESEARCH
(2019)
Article
Oncology
Ying Wang, M. Kathryn Leonard, Devin E. Snyder, Matthew L. Fisher, Richard L. Eckert, David M. Kaetzel
MOLECULAR CANCER RESEARCH
(2019)
Article
Multidisciplinary Sciences
Devin Snyder, Ying Wang, David M. Kaetzel
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Nidhi Pamidimukkala, Gemma S. Puts, M. Kathryn Leonard, Devin Snyder, Sandrine Dabernat, Edward C. De Fabo, Frances P. Noonan, Andrzej Slominski, Glenn Merlino, David M. Kaetzel
Summary: NME1 and NME2 genes have a significant impact on metastatic activity in a mouse model of melanoma, with NME2 showing MSG activity for the first time in a spontaneous cancer model, and NME1 displaying a novel metastasis-suppressor function in UVR-induced melanoma.
BRITISH JOURNAL OF CANCER
(2021)
Article
Biochemistry & Molecular Biology
Gemma Puts, Stuart Jarrett, Mary Leonard, Nicolette Matsangos, Devin Snyder, Ying Wang, Richard Vincent, Benjamin Portney, Rachel Abbotts, Lena McLaughlin, Michal Zalzman, Feyruz Rassool, David Kaetzel
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
M. Kathryn Leonard, Gemma S. Puts, Nidhi Pamidimukkala, Gautam Adhikary, Yili Xu, Eric Kwok, Yuxin Jin, Devin Snyder, Nicolette Matsangos, Marian Novak, Anup Mahurkar, Amol C. Shetty, Radomir M. Slominski, Edward C. De Fabo, Frances P. Noonan, Chi-Ping Day, Mohammed Rigi, Andrzej T. Slominski, Michelle G. Webb, David W. Craig, Glenn Merlino, Richard L. Eckert, John D. Carpten, Zarko Manojlovic, David M. Kaetzel
Summary: The study identified a family of genes that mediate the suppression of melanoma lung metastasis by analyzing melanomas from hepatocyte growth factor-overexpressing mice with a deletion of the Ink4a/p16 locus and hemizygous deletion of Nme1 and Nme2 metastasis suppressor genes. The increased lung metastatic activity was associated with missense mutations in eight signature genes, leading to a 32-gene signature strongly linked to lung metastatic potential. Expression profiles of these genes can predict improved survival of patients with skin or uveal melanoma. Silencing three representative genes from the signature in human melanoma cells increased invasive activity, suggesting their roles as mediators of metastasis suppression. These findings may have implications for prognostic markers and therapeutic targets in managing metastatic melanoma.
Correction
Biochemistry & Molecular Biology
M. Kathryn Leonard, Gemma S. Puts, Nidhi Pamidimukkala, Gautam Adhikary, Yili Xu, Eric Kwok, Yuxin Jin, Devin Snyder, Nicolette Matsangos, Marian Novak, Anup Mahurkar, Amol C. Shetty, Radomir M. Slominski, Edward C. De Fabo, Frances P. Noonan, Chi-Ping Day, Mohammed Rigi, Andrzej T. Slominski, Michelle G. Webb, David W. Craig, Glenn Merlino, Richard L. Eckert, John D. Carpten, Zarko Manojlovic, David M. Kaetzel
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)