Article
Biochemical Research Methods
Nicolas Castano, Sungu Kim, Adrian M. Martin, Stephen J. Galli, Kari C. Nadeau, Sindy K. Y. Tang
Summary: In this study, an integrated basophil isolation device (i-BID) was developed using microfluidics for rapid and high-purity separation of basophils from 100 μL of whole blood. The device includes a magnetic separation module that effectively captures non-basophils while preventing clogging. The i-BID achieved an average purity of 93.9% +/- 3.6% and a recovery rate of 95.6% +/- 3.4%, without causing degradation or unintentional activation of basophils. This research has important implications for basophil-based diagnostics in allergic disorders and other diseases.
Article
Biochemistry & Molecular Biology
Tito Palmela Leitao, Patricia Corredeira, Sandra Kucharczak, Margarida Rodrigues, Paulina Piairo, Carolina Rodrigues, Patricia Alves, Ana Martins Cavaco, Miguel Miranda, Marilia Antunes, Joao Ferreira, Jose Palma Reis, Tome Lopes, Lorena Dieguez, Luis Costa
Summary: Renal cell carcinoma (RCC) often presents as metastatic disease. Research on circulating tumor cells (CTCs) and liquid biopsies is improving understanding of RCC biology and metastasis formation. This study aimed to clinically validate the effectiveness of RUBYchip (TM), a microfluidic label-free CTC detection platform, in RCC patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Kai Zhao, Penglu Zhao, Jianhong Dong, Yunman Wei, Bin Chen, Yanjuan Wang, Xinxiang Pan, Junsheng Wang
Summary: This study demonstrates the successful isolation of circulating tumor cells (CTCs) from blood cells using a combination of dielectrophoresis and magnetophoresis in a microfluidic chip. The results show the potential application of this method in diagnostics.
Article
Biology
Jose Montoya Mira, Ajay A. Sapre, Brett S. Walker, Jesus Bueno Alvarez, Kyle T. Gustafson, Eugene Tu, Jared M. Fischer, Melissa H. Wong, Sadik Esener, Yu-Jui Chiu
Summary: Researchers have developed a label-free dielectrophoretic microfluidic platform for the enrichment of circulating hybrid cells (CHCs), showing promising potential for isolating tumor cells non-invasively from patient blood samples within one hour. They have optimized the device using in vitro models and successfully applied it to clinically relevant mutation analyses in 75% of the samples tested.
COMMUNICATIONS BIOLOGY
(2021)
Article
Engineering, Chemical
Xiangzhi Zhang, Xiawei Xu, Yong Ren, Yuying Yan, Aiguo Wu
Summary: An Y-Y shaped microfluidic device was designed to separate NSCLC cells using a dielectrophoresis approach, with a separation efficiency of around 99% achieved under optimal operating conditions. This study presents a potentially efficient, facile, and low-cost route for circulating tumor cell separation, which was optimized through numerical simulations.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Franciele Flores Vit, Rony Nunes, Yu Tzu Wu, Marco Cesar Prado Soares, Naiara Godoi, Eric Fujiwara, Hernandes F. Carvalho, Lucimara Gaziola de la Torre
Summary: This study introduces a microfluidic technology for drug screening that enables the assessment of cellular behavior in dynamic conditions and the recovery of cells for subsequent processing and imaging.
ANALYTICA CHIMICA ACTA
(2021)
Article
Materials Science, Multidisciplinary
Mengyuan Wang, Yulong Tan, Du Li, Gangwei Xu, Di Yin, Yunchao Xiao, Tiegang Xu, Xiaofeng Chen, Xiaoyue Zhu, Xiangyang Shi
Summary: The study developed a negative sorting method for the isolation of circulating tumor cells (CTCs) through a microfluidic platform integrated with streptavidin-functionalized electrospun polylactic-co-glycolic acid nanofibers. This method showed high efficiency in capturing white blood cells (WBCs) and enriching cancer cells, making it promising for cancer diagnosis.
ADVANCED FIBER MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Delaram Parvin, Zahra Sadat Hashemi, Farhad Shokati, Zahra Mohammadpour, Vahid Bazargan
Summary: By integrating magnetic nanoparticles with microfluidics, an effective and low-cost method for isolating HER2-positive breast cancer cells was developed, which improved the efficiency of cell isolation and reduced the analysis time. This microfluidic system offers a competitive solution for clinical applications.
Article
Chemistry, Analytical
Rohollah Nasiri, Amir Shamloo, Javad Akbari
Summary: Isolating circulating tumor cells (CTCs) from blood samples is crucial for cancer diagnosis and treatment, and microfluidics shows great potential in this area. Combining inertial and magnetic methods on a single chip was found to be effective in separating CTCs, with high recovery rates and purity levels.
Article
Chemistry, Analytical
Ali Doostmohammadi, Khaled Youssef, Shiva Akhtarian, Garrett Kraft, Pouya Rezai
Summary: This paper introduces a new sensor for rapid and low-cost detection of bacteria in water, which utilizes fluorescent magnetic CIP-MPs and a microfluidic channel for quick detection of Escherichia coli. The shape of the magnetic microstructures is optimized to enhance the accumulation of CIP-MPs in the microchannel. The sensor has low detection and quantification limits, and exhibits specificity towards Escherichia coli.
Article
Biochemical Research Methods
Jaewook Ryu, Junhyeong Kim, Ki-Ho Han
Summary: A disposable droplet generation microfluidic device (dDrop-Chip) is introduced in this study, which can control both droplet size and production rate in real time. The dDrop-Chip consists of a reusable sensing substrate and a disposable microchannel and integrates a droplet detector and a flow sensor on-chip. Experimental results demonstrate that the dDrop-Chip consistently generates monodisperse droplets with controlled size and production rate, making it a reliable and cost-effective technique for droplet-based applications.
Article
Chemistry, Analytical
Thaisa Aparecida Baldo, Camila dos Anjos Proenca, Fabiana da Silva Felix, Tayane Aguiar Freitas, Solange Kazumi Sakata, Lucio Angnes, Ronaldo Censi Faria
Summary: The study developed a new detection method using magnetic beads and antibodies to quickly and accurately detect ovalbumin in wine samples. This method was validated on five wine samples and showed excellent accuracy compared to enzyme-linked immunosorbent assay (ELISA).
Article
Biology
Jiahao Zhang, Jie Ren, Zirui LI, Yixing Gou
Summary: Circulating tumor cells (CTCs) are crucial biomarkers for liquid biopsies, playing a significant role in early cancer screening, prognosis, and real-time monitoring. Due to their low abundance in peripheral blood, the isolation of CTCs becomes necessary. The use of microfluidics for CTC sorting has gained attention due to its affordability, integration ease, low sample consumption, and advantages in manipulating micron-sized particles. This review summarizes the latest research on microfluidics-based CTC sorting, highlighting active sorting using external fields (electric, magnetic, acoustic, and optical tweezers) as well as passive sorting using flow effects in specific channel structures (microfiltration, deterministic lateral displacement, and inertial sorting). The advantages, limitations, and recent applications of each method are discussed, with a forward-looking perspective on future research.
Article
Materials Science, Characterization & Testing
F. A. Doronin, Yu Rudyak, G. O. Rytikov, A. G. Evdokimov, V. G. Nazarov
Summary: The paper demonstrates that by modifying the surface structure of PET substrates through multi-gas-phase treatment, the adhesion and wetting properties of PLA filament are significantly improved, enabling the production of planar microfluidic devices on them. Experimental results confirm significant changes in the chemical composition and nanorelief of PET substrates' surfaces, affecting the adhesion and wetting properties of the filament and enhancing the functional characteristics of the microfluidic devices.
Article
Biochemical Research Methods
Celine Macaraniag, Jian Zhou, Jing Li, William Putzbach, Nissim Hay, Ian Papautsky
Summary: Liquid biopsy has significant implications in cancer research and clinical applications, especially in preclinical studies for disease progression and treatment decision-making through the isolation of circulating tumor cells (CTCs). Microfluidic isolation systems play a crucial role in CTC isolation for cancer studies, diagnosis, and prognosis. However, there is a lack of validation studies on preclinical samples such as CTCs from mouse models.
