Article
Multidisciplinary Sciences
Jokubas Ausra, Micah Madrid, Rose T. Yin, Jessica Hanna, Suzanne Arnott, Jaclyn A. Brennan, Roberto Peralta, David Clausen, Jakob A. Bakall, Igor R. Efimov, Philipp Gutruf
Summary: Monitoring and control of cardiac function are crucial for studying cardiovascular pathophysiology and developing treatments. However, it is currently challenging to control the heart in freely moving small animal subjects. This study introduces a wireless battery-free device that enables real-time cardiac control and multisite stimulation, allowing optogenetic modulation of the entire rodent heart.
Article
Chemistry, Analytical
Bingsong Gu, Xiao Li, Cong Yao, Xiaoli Qu, Mao Mao, Dichen Li, Jiankang He
Summary: Recapitulating the morphology and electrophysiological activity of native myocardium is crucial for functional cardiac constructs. This study proposes a strategy to integrate platinum microelectrodes into cell-laden fibrin hydrogel to fabricate highly-aligned cardiac constructs and support electrophysiological recording. The constructs demonstrated close contact between the microelectrodes and cardiomyocytes, resulting in highly-aligned morphology. In addition, the embedded microelectrodes recorded changes in electrophysiological signals and altered cardiac behavior with electrical stimulation.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Yiran Lang, Rongyu Tang, Yafei Liu, Pengcheng Xi, Honghao Liu, Zhenzhen Quan, Da Song, Xiaodong Lv, Qiang Huang, Jiping He
Summary: A system with various electrodes capable of recording a large spectrum of electrophysiological signals from the cortex, spinal cord, peripheral nerves, and muscles of freely moving animals was built. The system was found to be stable and applicable for multiple behavior conditions, supporting previously inaccessible research of neural injury, rehabilitation, brain-inspired computing, and fundamental neuroscience.
Article
Engineering, Biomedical
Roberta Visone, Giovanni S. Ugolini, Daniela Cruz-Moreira, Simona Marzorati, Stefano Piazza, Enrico Pesenti, Alberto Redaelli, Matteo Moretti, Paola Occhetta, Marco Rasponi
Summary: Cardiac toxicity is a common adverse outcome in drug development that can lead to withdrawal of drugs from the market. Recent advancements in developing in vitro models resembling the human heart have allowed for more accurate detection of drug-induced cardiac toxicity early in the drug development process. Organs-on-chip are considered promising tools to replicate key aspects of in vivo cardiac physiology and provide a means for direct analysis of functional outputs.
Article
Chemistry, Analytical
Eunji Hong, Christopher Glynn, Qianbin Wang, Siyuan Rao
Summary: Electroretinography (ERG) is a non-invasive electrophysiological recording technique used to detect neuronal cell signaling in the visual system. We have developed an advanced ERG recording system integrated with a programmable optogenetic stimulation method, which allows for the dissection of neural dynamics under different physiological and pathological conditions.
Article
Chemistry, Analytical
Rongke Gao, Feng Chen, Dongdong Yang, Lihua Zheng, Tongmei Jing, Huakun Jia, Xiaozhe Chen, Yang Lu, Shenghao Xu, Dongzhi Zhang, Liandong Yu
Summary: We developed a new pump-free hybrid microfluidic chip for simultaneous detection of creatine kinase MB isoenzyme and cardiac troponin cardiac markers. The integration of paper and PDMS microfluidic chip offers portability, low-cost, and high sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Materials Science, Biomaterials
Kirstie Belanger, Abigail N. Koppes, Ryan A. Koppes
Summary: Excitation-coupling (ECC) is crucial for maintaining cardiac output, but the contribution of nonmuscle cells to ECC has been overlooked. Developing simple and biomimetic in vitro models is necessary to better understand and prevent physiological dysfunction. This review highlights the recent developments in microphysiological systems.
Review
Biochemistry & Molecular Biology
Oscar Casis, Leire Echeazarra, Beatriz Saenz-Diez, Monica Gallego
Summary: Hypothyroidism is a common endocrine disorder, especially in women. Apart from overt hypothyroidism, subclinical hypothyroidism is also prevalent. This condition affects cardiac electrical activity, particularly during the repolarization phase, increasing the susceptibility to cardiac arrhythmias. The traditional view attributing these effects to thyroid hormone deficiency is being challenged by recent studies, which demonstrate the crucial role of TSH in cardiac electrical remodeling. Therefore, different types of hypothyroidism can result in varying forms of cardiac electrical remodeling, raising the question of redefining TSH reference ranges.
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Tomoaki Banno, Shuhei Tsuruhara, Yu Seikoba, Ryohei Tonai, Koji Yamashita, Shinnosuke Idogawa, Yuto Kita, Ko Suzuki, Yuki Yagi, Yuki Kondo, Rika Numano, Kowa Koida, Takeshi Kawano
Summary: Microscale needle-like electrodes offer high spatiotemporal resolution for in vivo extracellular recording. However, reducing the size to the nanoscale increases electrical impedance. By stacking a nanoelectrode on an amplifier module, the quality of neuronal signal recording can be improved. In experiments conducted on the mouse cortex, the nanoelectrode with amplifier could record a wider range of neural signals compared to the electrode without the amplifier.
Article
Nanoscience & Nanotechnology
Shihong Xu, Yu Deng, Jinping Luo, Enhui He, Yaoyao Liu, Kui Zhang, Yan Yang, Shengwei Xu, Longze Sha, Yilin Song, Qi Xu, Xinxia Cai
Summary: By using a high-performance microelectrode array, the study investigates and activates the learning and memory functions of neurons. Electrical stimulation can change the firing pattern of neurons and improve the correlation and synchrony of the networks.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Materials Science, Multidisciplinary
Zhiyuan Chen, Khanh Nguyen, Grant Kowalik, Xinyu Shi, Jinbi Tian, Mitansh Doshi, Bridget R. R. Alber, Xun Guan, Xitong Liu, Xin Ning, Matthew W. W. Kay, Luyao Lu
Summary: Transparent microelectrodes have been widely studied in the biomedical field due to their advantages in simultaneous electrical and optical interrogation of cell/tissue activity. In this study, a microelectrode array (MEA) with gold-coated silver nanowires (Au-Ag NWs) was designed to achieve desirable mechanical stretchability, optical transparency, electrochemical performance, and chemical stability. The MEAs exhibited high optical transparency, low electrochemical impedance, stable chemical and electromechanical performance, and superior capability for colocalized electrophysiological and optical mapping of cardiac function.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Multidisciplinary
Yuecheng Zhou, Erica Liu, Yang Yang, Felix S. Alfonso, Burhan Ahmed, Kenneth Nakasone, Csaba Forro, Holger Muller, Bianxiao Cui
Summary: Optical recording based on voltage-sensitive electrochromic materials allows for long-term and sensitive measurement of bioelectrical signals.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Engineering, Biomedical
Feng Zhang, Hongyi Cheng, Kaiyun Qu, Xuetian Qian, Yongping Lin, Yike Zhang, Sichong Qian, Ningping Huang, Chang Cui, Minglong Chen
Summary: Heart-on-chip is a potential tool for cardiac tissue engineering that mimics key physiological cues in cardiac pathophysiology. This study presents a novel heart-on-chip platform with soft conductive hydrogel pillar electrodes, which allows for controlled electrical stimulation and direct functional readouts. The platform successfully improves the functional performance of cardiac tissues by altering tissue structure dynamics and contractile development, and accurately measures contractile forces through optical tracking of hydrogel pillar displacement. The conductive properties of the hydrogel pillars enable non-invasive electrophysiology studies, enabling real-time monitoring of signal changes and drug administration to the cardiac tissues.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Analytical
Saeyeong Jeon, Youjin Lee, Daeho Ryu, Yoon Kyung Cho, Yena Lee, Sang Beom Jun, Chang-Hyeon Ji
Summary: Optogenetics has become crucial in neuroscience research due to its cell-type-specific neuromodulation feature. Technological advances in light delivery devices have allowed for the combination of optogenetics and electrophysiology, as demonstrated by a novel optrode array design for optical modulation and electrophysiological recording. In vivo experiments with transgenic mice expressing channelrhodopsin have confirmed the effectiveness of optical activation and neural recording.
Article
Toxicology
Roberta Visone, Ferran Lozano-Juan, Simona Marzorati, Massimo Walter Rivolta, Enrico Pesenti, Alberto Redaelli, Roberto Sassi, Marco Rasponi, Paola Occhetta
Summary: Determining the potential cardiotoxicity and pro-arrhythmic effects of drug candidates is crucial in drug development. This study investigates the use of uHeart, a 3D miniaturized in vitro model of human myocardium, to evaluate drug-induced QT alterations and arrhythmic events. The results show that uHeart is capable of accurately predicting clinical outcomes and detecting cardiotoxic drug concentrations, making it a promising preclinical tool for cardiotoxicity studies.
TOXICOLOGICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Anna M. Belle, Heather A. Enright, Ana Paula Sales, Kristen Kulp, Joanne Osburn, Edward A. Kuhn, Nicholas O. Fischer, Elizabeth K. Wheeler
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Heather A. Enright, Miranda J. S. Falso, Michael A. Malfatti, Victoria Lao, Edward A. Kuhn, Nicholas Hum, Yilan Shi, Ana Paula Sales, Kurt W. Haack, Kristen S. Kulp, Bruce A. Buchholz, Gabriela G. Loots, Graham Bench, Kenneth W. Turteltaub
Article
Multidisciplinary Sciences
D. Soscia, A. Belle, N. Fischer, H. Enright, A. Sales, J. Osburn, W. Benett, E. Mukerjee, K. Kulp, S. Pannu, E. Wheeler
Article
Biochemical Research Methods
Doris Lam, Heather A. Enright, Sandra K. G. Peters, Monica L. Moya, David A. Soscia, Jose Cadena, Javier A. Alvarado, Kristen S. Kulp, Elizabeth K. Wheeler, Nicholas O. Fischer
JOURNAL OF NEUROSCIENCE METHODS
(2020)
Article
Biochemical Research Methods
Jose Cadena, Ana Paula Sales, Doris Lam, Heather A. Enright, Elizabeth K. Wheeler, Nicholas O. Fischer
PLOS COMPUTATIONAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Heather A. Enright, Doris Lam, Aimy Sebastian, Ana Paula Sales, Jose Cadena, Nicholas R. Hum, Joanne J. Osburn, Sandra K. G. Peters, Bryan Petkus, David A. Soscia, Kristen S. Kulp, Gabriela G. Loots, Elizabeth K. Wheeler, Nicholas O. Fischer
SCIENTIFIC REPORTS
(2020)
Review
Pharmacology & Pharmacy
Charles P. France, Gerard P. Ahern, Saadyah Averick, Alex Disney, Heather A. Enright, Babak Esmaeli-Azad, Arianna Federico, Lisa R. Gerak, Stephen M. Husbands, Benedict Kolber, Edmond Y. Lau, Victoria Lao, David R. Maguire, Michael A. Malfatti, Girardo Martinez, Brian P. Mayer, Marco Pravetoni, Niaz Sahibzada, Phil Skolnick, Evan Y. Snyder, Nestor Tomycz, Carlos A. Valdez, Jim Zapf
Summary: The only medication available to prevent and treat opioid overdose is naloxone, approved by the FDA nearly 50 years ago. Due to its limitations, a scientific meeting was convened by NIAID/NIH to explore emerging alternative approaches for treating opioid overdose in the event of weaponization of synthetic opioids. This meeting discussed new approaches such as intranasal nalmefene, methocinnamox, covalent naloxone nanoparticles, serotonin receptor agonists, fentanyl-binding cyclodextrin scaffolds, detoxifying biomimetic nanosponge decoy receptors, and antibody-based strategies.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Multidisciplinary Sciences
Brian J. Bennion, Michael A. Malfatti, Nicholas A. Be, Heather A. Enright, Saphon Hok, C. Linn Cadieux, Timothy S. Carpenter, Victoria Lao, Edward A. Kuhn, M. Windy McNerney, Felice C. Lightstone, Tuan H. Nguyen, Carlos A. Valdez
Summary: Recent incidents have highlighted a resurgence in the use of nerve agents, emphasizing the importance of antidote development. The standard treatment against these agents relies on small molecule oximes that can restore AChE activity effectively but are limited to the peripheral nervous system. Efforts to develop a novel CNS-permeable oxime reactivator are underway to address this limitation.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Doris Lam, Nicholas O. Fischer, Heather A. Enright
Summary: Recent advancements in microphysiological systems have focused on reconstructing key elements found in the brain and improving technologies to detect activity of electrogenic cells forming neural networks, with particular emphasis on three-dimensional multielectrode arrays (3D MEAs). These 3D MEAs provide a means to monitor neural network activity in all three dimensions of engineered tissue, overcoming current technical challenges in monitoring in vitro systems.
CURRENT OPINION IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Nicholas D. Henkel, Marissa A. Smail, Xiaojun Wu, Heather A. Enright, Nicholas Fischer, Hunter M. Eby, Robert E. McCullumsmith, Rammohan Shukla
Summary: The study identified 119 genes associated with epilepsy and revealed their impact on homeostatic and synaptic pathways. Analysis of cellular proportions showed opposing changes in different cell types. A Bayesian network analysis demonstrated aberrant physiological interactions between nonneuronal cells and Vip interneurons in driving seizures.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
David A. Soscia, Doris Lam, Angela C. Tooker, Heather A. Enright, Michael Triplett, Piyush Karande, Sandra K. G. Peters, Ana Paula Sales, Elizabeth K. Wheeler, Nicholas O. Fischer
Review
Environmental Sciences
Michael A. Malfatti, Bruce A. Buchholz, Heather A. Enright, Benjamin J. Stewart, Ted J. Ognibene, A. Daniel McCartt, Gabriela G. Loots, Maike Zimmermann, Tiffany M. Scharadin, George D. Cimino, Brian A. Jonas, Chong-Xian Pan, Graham Bench, Paul T. Henderson, Kenneth W. Turteltaub
Article
Multidisciplinary Sciences
Doris Lam, Heather A. Enright, Jose Cadena, Sandra K. G. Peters, Ana Paula Sales, Joanne J. Osburn, David A. Soscia, Kristen S. Kulp, Elizabeth K. Wheeler, Nicholas O. Fischer
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Michael A. Malfatti, Heather A. Enright, Nicholas A. Be, Edward A. Kuhn, Saphon Hok, M. Windy McNerney, Victoria Lao, Tuan H. Nguyen, Felice C. Lightstone, Timothy S. Carpenter, Brian J. Bennion, Carlos A. Valdez
CHEMICO-BIOLOGICAL INTERACTIONS
(2017)
Proceedings Paper
Engineering, Biomedical
Anna M. Belle, Heather A. Enright, Erik V. Mukerjee, David A. Soscia, Joanne J. Osburn, Edward A. Kuhn, Kristen S. Kulp, Elizabeth K. Wheeler, Vanessa M. Tolosa
2017 IEEE INTERNATIONAL SYMPOSIUM ON MEDICAL MEASUREMENTS AND APPLICATIONS (MEMEA)
(2017)
