Journal
LAB ON A CHIP
Volume 17, Issue 13, Pages 2186-2192Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7lc00283a
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Funding
- NIH-NCI [R01 CA181595]
- Simons Foundation [519054]
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We present a novel method for conducting true single-cell encapsulation at very high efficiency for the manipulation of precious samples. Our unique strategy is based on the sequential capture and original encapsulation of single-cells into a series of hydrodynamic traps. We identified two distinct modes of encapsulation and we established their associated design rules. We improved the trapping scheme to reach a near perfect capture efficiency and make it compatible with the encapsulation process. Finally, we developed the complete device operation that permits highly efficient single-cell encapsulation and droplet retrieval. This platform provides the foundation to a fully integrated multiparameter platform that will impact the analysis of tissues at single-cell resolution.
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