Review
Immunology
Yinxi Zhou, Jinghua Xia, Shuonan Xu, Tao She, Yanning Zhang, Ying Sun, Miaomiao Wen, Tao Jiang, Yanlu Xiong, Jie Lei
Summary: The development and growth of tumors pose a significant and ongoing threat to human life globally. Despite the remarkable progress achieved by advanced therapeutic strategies such as immune checkpoint therapy and CAR-T in treating solid and hematological malignancies, the malignant initiation and progression of cancer remains controversial and requires further research. Experimental animal models not only have great advantages in simulating tumor occurrence, development, and malignant transformation mechanisms, but also can be used to evaluate the therapeutic effects of diverse clinical interventions, gradually becoming indispensable in cancer research. This paper reviews recent research progress in mouse and rat models, focusing on spontaneous, induced, transgenic, and transplantable tumor models, aiming to provide guidance for future studies on malignant mechanisms and tumor prevention.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Neurosciences
Garikoitz Azkona, Rosario Sanchez-Pernaute
Summary: Animal models are crucial in neuroscience research, but there are recurring issues in data collection and interpretation, a lack of biomarkers, and an over-reliance on mice, leading to low translation efficiency. Non-human primates, with their higher brain complexity and similarity to humans, can provide more relevant translational information. Therefore, resources for training, education, husbandry, and data sharing should be prioritized to improve translational efficiency.
PROGRESS IN NEUROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alex R. Brown, Hannah E. Branthwaite, Zahra Z. Farahbakhsh, Snigdha Mukerjee, Patrick R. Melugin, Keaton Song, Habiba Noamany, Cody A. Siciliano
Summary: There is tension between methodologies in model species research and preclinical to clinical translation in alcohol research. To address this, researchers establish a modular alcohol reinforcement paradigm and combine it with existing preclinical alcohol models to determine longitudinal phenotype dynamics and potential neuro-biomarkers of alcohol vulnerability.
MOLECULAR PSYCHIATRY
(2023)
Review
Biochemistry & Molecular Biology
Alesa H. Netzley, Galit Pelled
Summary: In recent years, pigs have gained attention as an important model animal for studying human neuroanatomy, physiology, and behavior. Their high intelligence and ability to perform various behaviors make them valuable in understanding neurological conditions. Pigs have particularly emerged as a relevant species for studying traumatic brain injury. The use of pigs in cognitive and behavioral research, as well as physiological assessment, is discussed in this review.
Review
Biotechnology & Applied Microbiology
Hye Sook Shin, Heather Hyeyoon Shin, Yasuhiro Shudo
Summary: Establishing an appropriate disease model, frequent modifications to surgical procedures, and insufficient transparency in reporting are three major inhibitors in current preclinical myocardial infarction research.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Clinical Neurology
Sarinnapha M. Vasunilashorn, Nadia Lunardi, John C. Newman, Gregory Crosby, Leah Acker, Ted Abel, Seema Bhatnagar, Colm Cunningham, Rafael de Cabo, Laura Dugan, Joseph A. Hippensteel, Yumiko Ishizawa, Shouri Lahiri, Edward R. Marcantonio, Zhongcong Xie, Sharon K. Inouye, Niccolo Terrando, Roderic G. Eckenhoff
Summary: Delirium is a common, morbid, and costly syndrome closely associated with Alzheimer's disease (AD) and AD-related dementias (ADRD). While human studies have improved our understanding of delirium in terms of incidence, risk factors, outcomes, and management, limited knowledge exists about the neurobiology of delirium. This article discusses the use of preclinical and translational animal models to advance our understanding of delirium neurobiology and develop new prevention and treatment strategies. Recommendations for the development and validation of these models are provided to enhance awareness in this important field.
ALZHEIMERS & DEMENTIA
(2023)
Review
Multidisciplinary Sciences
Lara A. Ray, Steven J. Nieto, Erica N. Grodin
Summary: Alcohol and substance use disorders pose challenges in effective treatment due to their heterogeneous nature. Efforts to classify addiction subtypes and apply neuroscience frameworks have the potential to advance precision medicine in this field. The use of deep behavioral phenotyping in neuroscience-informed batteries shows promise for understanding addiction on a clinical neuroscience level.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Jonaid Ahmad Malik, Zahid Yaseen, Lahari Thotapalli, Sakeel Ahmed, Mohd Farooq Shaikh, Sirajudheen Anwar
Summary: Schizophrenia is a complex mental disorder caused by an imbalance in neurotransmitters. Animal models based on dopamine, glutamate, and serotonin have been developed to understand the pathophysiology of the disorder and develop new therapeutic approaches. Genetic factors have also been identified, and new targets like the orexin system and cannabinoid receptors are being explored for treatment. Non-pharmacological models have been developed to mimic the symptoms of schizophrenia and test new drug therapies. This review highlights the available animal models and behavioral tests in the study of schizophrenia.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Tatiana O. Kolesnikova, Konstantin A. Demin, Fabiano Costa, Konstantin N. Zabegalov, Murilo S. de Abreu, Elena Gerasimova, Allan Kalueff
Summary: Channelopathies are systemic disorders associated with dysfunctional ion channels. The zebrafish has become a popular model organism for studying these disorders, but there are still challenges to overcome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Behavioral Sciences
Murilo S. de Abreu, Ana C. V. V. Giacomini, Rafael Genario, Bruna E. dos Santos, Leticia Marcon, Konstantin A. Demin, David S. Galstyan, Tatiana Strekalova, Tamara G. Amstislavskaya, Allan Kalueff
Summary: Color is an important environmental factor that affects human and animal behavior and physiology in multiple ways. Zebrafish has emerged as an important model species in neuroscience research, with growing utility in studying color neurobiology and neurobehavioral phenomics. Future research directions in this field include exploring the role of colors in zebrafish models and their impact on CNS disease modeling and animal welfare.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2021)
Review
Neurosciences
Murilo S. de Abreu, Fabiano Costa, Ana C. V. V. Giacomini, Konstantin A. Demin, Elena Petersen, Denis B. Rosemberg, Allan Kalueff
Summary: Although traditional medicine has been practiced for a long time, its therapeutic mechanisms are not fully understood. This study highlights the importance of using zebrafish as a model organism to study the neurotropic effects of traditional medicine and their potential in treating brain disorders.
CURRENT NEUROPHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tiphaine Raia-Barjat, Margaux Digonnet, Antoine Giraud, Taghreed Ayash, Seline Vancolen, Mohamed Benharouga, Celine Chauleur, Nadia Alfaidy, Guillaume Sebire
Summary: Preterm birth and chorioamnionitis have significant impacts on both infant and maternal health. Effective treatments and diagnostic methods are still lacking. Animal models can provide insights into the underlying mechanisms and aid in the development of novel therapies.
Review
Cardiac & Cardiovascular Systems
Max L. A. Ebert, Vanessa F. Schmidt, Lena Pfaff, Anne von Thaden, Melanie A. Kimm, Moritz Wildgruber
Summary: Restenosis following interventional recanalization procedures is influenced by various mechanical and biological processes triggered by vascular trauma. The most serious process is neointimal hyperplasia, which can be traced back to endothelial damage, monocyte accumulation, fibroblast migration, and smooth muscle cell transformation. Different animal models have their own advantages and disadvantages, and choosing the proper model requires consideration of species-specific biological properties.
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2021)
Review
Urology & Nephrology
Neil A. Hukriede, Danielle E. Soranno, Veronika Sander, Tayla Perreau, Michelle C. Starr, Peter S. T. Yuen, Leah J. Siskind, Michael P. Hutchens, Alan J. Davidson, David M. Burmeister, Sarah Faubel, Mark P. de Caestecker
Summary: Models used in preclinical research often have limitations that result in results inconsistent with clinical practice. In the field of acute kidney injury (AKI), clinical trial failures are mainly attributed to inaccurate modeling in rodents. Currently, emerging research methods such as human kidney organoids, zebrafish, rodent, and large animal models are being applied in preclinical therapeutic research. However, the traditional preclinical research methods often overlook the limitations of model systems, leading to misleading results in the preclinical stage of treatment effectiveness. To address this problem, a more flexible and integrated approach is needed, with investigator teams using different model systems closely collaborating with clinical researchers to develop more targeted treatment methods. This review summarizes the current state of acute kidney injury model development and emphasizes the importance of adopting a multidisciplinary approach to optimize the development of treatment methods for acute kidney injury.
NATURE REVIEWS NEPHROLOGY
(2022)
Article
Engineering, Biomedical
Diana Szucs, Zsolt Fekete, Melinda Guba, Lajos Kemeny, Katalin Jemnitz, Emese Kis, Zoltan Vereb
Summary: The importance of 3D models in pharmacological tests and personalized therapies is significant, allowing insight into cell responses and suitable for toxicological testing. They are crucial in personalized and regenerative medicine for safe and effective treatment, providing the opportunity to select the most appropriate drug for the patient. These models have the potential to replace animal models in the future and have applications in applied and basic research due to their similarity to native tissue responses.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Neurosciences
Barbara D. Fontana, Talise E. Muller, Madeleine Cleal, Murilo S. de Abreu, William H. J. Norton, Konstantin A. Demin, Tamara G. Amstislavskaya, Elena Petersen, Allan Kalueff, Matthew O. Parker, Denis B. Rosemberg
Summary: Social behavior plays a crucial role in maintaining health and wellbeing, while dysfunctional interactions pose risks for physical and psychiatric disorders. Despite extensive research on the effects of positive and negative social interactions, the underlying mechanisms remain unclear. Zebrafish, with well-characterized social behavior phenotypes and genetic similarities to humans, offer a promising model for studying the fundamental mechanisms of social interactions.
PROGRESS IN NEUROBIOLOGY
(2022)
Review
Neurosciences
Fabiano Costa, Luiz Rosa, Vanessa A. Quadros, Murilo S. de Abreu, Adair R. S. Santos, Lynne U. Sneddon, Allan Kalueff, Denis B. Rosemberg
Summary: This article introduces the advantages of zebrafish as a pain model and its applications in studying the mechanisms of pain response. The article also discusses the latest research advances and outlines the potential advantages and limitations of zebrafish models in studying pain behaviors and mood disorders, as well as facilitating analgesic therapy screening.
CURRENT NEUROPHARMACOLOGY
(2022)
Review
Neurosciences
Murilo S. de Abreu, Fabiano Costa, Ana C. V. V. Giacomini, Konstantin A. Demin, Elena Petersen, Denis B. Rosemberg, Allan Kalueff
Summary: Although traditional medicine has been practiced for a long time, its therapeutic mechanisms are not fully understood. This study highlights the importance of using zebrafish as a model organism to study the neurotropic effects of traditional medicine and their potential in treating brain disorders.
CURRENT NEUROPHARMACOLOGY
(2022)
Review
Clinical Neurology
Murilo S. de Abreu, Fabiano Costa, Ana C. V. V. Giacomini, Konstantin A. Demin, Konstantin N. Zabegalov, Gleb O. Maslov, Yuriy M. Kositsyn, Elena Petersen, Tatiana Strekalova, Denis B. Rosemberg, Allan Kalueff
Summary: Mood disorders, particularly depression, are a leading cause of disability in humans. Anhedonia, the loss of pleasure, is a common and severe symptom of clinical depression. Experimental animal models are used extensively to understand the mechanisms of depression and develop new treatments, with rodents being widely validated. Zebrafish, as a model organism, is emerging as a powerful tool for studying affective disorders and modeling anhedonia, offering potential for translational research.
INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY
(2022)
Letter
Veterinary Sciences
Murilo S. de Abreu, Ana C. V. V. Giacomini, Konstantin A. Demin, Elena V. Petersen, Allan V. Kalueff
Article
Neurosciences
Murilo S. de Abreu, Ana C. V. V. Giacomini, Rafael Genario, Konstantin A. Demin, Tamara G. Amstislavskaya, Fabiano Costa, Denis B. Rosemberg, Lynne U. Sneddon, Tatyana Strekalova, Marta C. Soares, Allan Kalueff
Summary: Pain is critical for organismal survival, evoking strong physiological and behavioral responses in various species. However, the long-term effects of early-life pain exposure on human and animal emotional responses remain poorly understood, indicating a need for further research in this area.
NEUROSCIENCE LETTERS
(2022)
Article
Neurosciences
Leticia Marcon, Ana C. V. V. Giacomini, Bruna E. dos Santos, Fabiano Costa, Denis B. Rosemberg, Konstantin A. Demin, Allan V. Kalueff, Murilo S. de Abreu
Summary: Sex is an important variable in translational biomedical research, and there may be sex differences in zebrafish behavioral responses to pain and fear stimuli.
NEUROSCIENCE LETTERS
(2022)
Article
Cell Biology
Evgeniy Svirin, Ekaterina Veniaminova, Joao Pedro Costa-Nunes, Anna Gorlova, Aleksei Umriukhin, Allan Kalueff, Andrey Proshin, Daniel C. Anthony, Andrey Nedorubov, Anna Chung Kwan Tse, Susanne Walitza, Lee Wei Lim, Klaus-Peter Lesch, Tatyana Strekalova
Summary: The interaction between brain serotonin deficiency and environmental adversity leads to excessive aggression in females. Complete inactivation of the gene encoding tryptophan hydroxylase-2 results in the absence of neuronal serotonin synthesis and aggression in male and female mice. Moderate reduction in brain serotonin levels and exposure to stress also lead to increased aggression in male mice. Furthermore, changes in gene expression and myelination-related processes may underlie the molecular mechanisms of aggressive behavior.
Article
Pharmacology & Pharmacy
Maria A. Tikhonova, Nikolai A. Maslov, Alim A. Bashirzade, Eugenyi V. Nehoroshev, Vladislav Y. Babchenko, Nadezhda D. Chizhova, Elena O. Tsibulskaya, Anna A. Akopyan, Evgeniya V. Markova, Yi-Ling Yang, Kwok-Tung Lu, Allan V. Kalueff, Lyubomir I. Aftanas, Tamara G. Amstislavskaya
Summary: Researchers developed a laser-induced brain injury model in zebrafish, which successfully replicated behavioral and neuroanatomical changes observed in traumatic brain injury (TBI). The model showed similarities to mammalian TBI models, validating its effectiveness. The study also examined the changes in neurons and brain-derived neurotrophic factor (BDNF) after the injury, as well as the activation of microglia and the levels of hypoxia-inducible factor 1-alpha (Hif1a).
Review
Biochemistry & Molecular Biology
Evgeniy Svirin, Johannes de Munter, Aleksei Umriukhin, Elisaveta Sheveleva, Allan Kalueff, Andrei Svistunov, Sergey Morozov, Susanne Walitza, Tatyana Strekalova
Summary: Gangliosides play important roles in neural development and function, and their dysfunction is associated with neurological disorders and psychiatric conditions. Abnormal ganglioside function can result in neuroinflammation, aberrant myelination, and altered insulin receptor signaling. Understanding the role of gangliosides in developmental neuropsychiatric disorders is of great clinical significance.
Review
Biochemistry & Molecular Biology
Tatiana O. Kolesnikova, Konstantin A. Demin, Fabiano Costa, Konstantin N. Zabegalov, Murilo S. de Abreu, Elena Gerasimova, Allan Kalueff
Summary: Channelopathies are systemic disorders associated with dysfunctional ion channels. The zebrafish has become a popular model organism for studying these disorders, but there are still challenges to overcome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ilya S. Zhukov, Inessa Karpova, Nataliya A. Krotova, Ilya Y. Tissen, Konstantin A. Demin, Petr D. Shabanov, Evgeny A. Budygin, Allan Kalueff, Raul R. Gainetdinov
Summary: The study characterizes the behavioral and neurochemical phenotypes of TAAR1 knockout mice, finding that these mice exhibit increased aggression in the resident-intruder test, reduced self-grooming behavior in the novelty-induced grooming test, and higher cortical serotonin levels. The findings suggest a potential association between TAAR1 and aggression-related neuropsychiatric disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Fabiano V. Costa, Tatiana O. Kolesnikova, David S. Galstyan, Nikita P. Ilyin, Murilo S. de Abreu, Elena V. Petersen, Konstantin A. Demin, Konstantin B. Yenkoyan, Allan V. Kalueff
Summary: Psychiatric disorders are prevalent brain pathologies. Reliable animal models are needed for effective treatment. Zebrafish show complex behaviors similar to rodents and humans, making them suitable for modeling psychiatric disorders. However, there are challenges and the field needs further discussion and consideration of clinical prevalence and pathological complexity. Recent developments in molecular biology might pave the way for wider use of zebrafish in CNS disease modeling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Anton D. D. Shevlyakov, Tatiana O. O. Kolesnikova, Murilo S. S. de Abreu, Elena V. V. Petersen, Konstantin B. B. Yenkoyan, Konstantin A. A. Demin, Allan V. V. Kalueff
Summary: Epilepsy is a neurological disorder characterized by seizures and neuronal hyperactivity. The exact pathogenesis is not fully understood, but genetic factors are known to play a key role. In this study, a computational approach was used to generate a comprehensive network of molecular pathways associated with epilepsy. The study identified potential key interactors and revealed functional molecular pathways, highlighting the importance of targeting downstream pathways for more effective treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Veterinary Sciences
Maria M. Kotova, David S. Galstyan, Tatiana O. Kolesnikova, Murilo S. de Abreu, Tamara G. Amstislavskaya, Tatyana Strekalova, Elena V. Petersen, Konstantin B. Yenkoyan, Konstantin A. Demin, Allan V. Kalueff
Summary: Antimicrobial drugs can influence the central nervous system and gut microbiota, leading to changes in brain and behavior. Zebrafish models are useful for studying the effects of these drugs on the central nervous system. Recent research has revealed the impacts of a wide range of antimicrobial drugs on brain and behavior in zebrafish.
VETERINARY SCIENCES
(2023)