Article
Cell Biology
Shiho Tanaka, C. Anders Olson, Christopher O. Barnes, Wendy Higashide, Marcos Gonzalez, Justin Taft, Ashley Richardson, Marta Martin-Fernandez, Dusan Bogunovic, Priyanthi N. P. Gnanapragasam, Pamela J. Bjorkman, Patricia Spilman, Kayvan Niazi, Shahrooz Rabizadeh, Patrick Soon-Shiong
Summary: As SARS-CoV-2 variants continue to emerge, scientists have utilized mRNA display technology to successfully discover neutralizing antibodies that can combat the virus broadly. Through structural studies of the S1 subunit of the S glycoprotein, various binding modes for antibodies targeting rare epitopes in the RBD and S1 domain have been revealed. They have also engineered a potent antibody that can bind to SARS-CoV-2 variants with specific substitutions.
Article
Chemistry, Analytical
M. Zaki, Ezz El Shafie, Soha A. Abdel-Gawad, Amany M. Fekry, Renad S. El-Kamel, M. Shehata
Summary: A novel electrochemical sensor for nicotine detection has been successfully prepared by depositing manganese/copper nanoparticles on the surface of a carbon paste electrode. This sensor showed good catalytic activity for the anodic oxidation of nicotine in potassium phosphate buffer solutions of different pH values. It exhibited high sensitivity and stability for detecting nicotine in urine and tobacco samples.
Article
Electrochemistry
Seyed Karim Hassaninejad-Darzi, Behnaz Aghamohseni
Summary: Providing an efficient and low-cost electrocatalyst is a significant challenge in direct hydrazine fuel cells (DHFC). In this study, Mn(II)-Y nanozeolite was synthesized and used for electrocatalytic oxidation of hydrazine. The modified electrode showed a significant decrease in overvoltage of N2H4 electro-oxidation compared to the bare carbon paste electrode. The overall reaction mechanism indicated the formation of environmentally friendly N-2 and H2O as end products.
JOURNAL OF APPLIED ELECTROCHEMISTRY
(2023)
Article
Environmental Sciences
Anil Patyal, Jatinder Paul Singh Gill, Jasbir Singh Bedi, Rabinder Singh Aulakh
Summary: Aflatoxins, major contaminants in animal feed, were found in a high percentage of dairy animal concentrate feed samples, with some exceeding tolerance limits. Seasonal variations were observed in the levels of aflatoxins contamination.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Microbiology
Anirban Bhattacharyya, Mohd Kamran, Sarfaraz Ahmad Ejazi, Sonali Das, Nicky Didwania, Rahul Bhattacharjee, Mehebubar Rahaman, Rama Prosad Goswami, Krishna Pandey, Vidya Nand Ravi Das, Pradeep Das, Saswati Gayen, Nahid Ali
Summary: This study explored the role of RDK2 as a potential diagnostic marker for visceral leishmaniasis (VL). In silico analysis predicted that RDK2 is conserved among Leishmania species. The experimental results demonstrated that RDK2 could be a candidate biomarker for the diagnosis of VL using serum and urine samples.
Article
Infectious Diseases
Delphine Girlich, Souad Ouzani, Isabelle Langlois, Nicolas Fortineau, Thierry Naas, Laurent Dortet
Summary: The study demonstrates that using molecular biology tests on enriched culture media can improve the early detection rate of patients colonized with carbapenemase-producing Enterobacterales at low levels, while saving detection time.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Microbiology
Stathis D. Kotsakis, Anastasia Lambropoulou, Georgios Miliotis, Eva Tzelepi, Vivi Miriagou, Leonidas S. Tzouvelekis
Summary: In the absence of a molecule that would collectively inhibit both metallo-β-lactamases and serine-reactive carbapenemases, containment of carbapenemase genes is the main strategy for addressing carbapenem resistance in hospitals. However, there is a need for cost-effective methods to rapidly detect carbapenemase-producing enterobacteria (CPE) to facilitate containment measures. This study developed a low-cost CPE detection method based on direct colorimetry, which showed high sensitivity and specificity in detecting CPE.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Fu-Chun Hsueh, Sheng-Yuan Wang, Wei-Hao Lin, Chuen-Fu Lin, Chen-Yu Tsai, Chin-Wen Huang, Ning Sun, Ming-Tang Chiou, Chao-Nan Lin
Summary: The study found that piglets with superior maternal-transferred NAbs had delayed and relatively slight viral loads in serum, and overall higher survival rates against wild PRRSV infections. There was a positive correlation of maternal NAbs between sows and their piglets; furthermore, high NAbs titers in piglets could last for at least 4 weeks.
Article
Public, Environmental & Occupational Health
Muthusamy Santhosh Kumar, P. Kamaraj, Siraj Ahmed Khan, Ramesh Reddy Allam, Pradip V. Barde, Bhagirathi Dwibedi, Suman Kanungo, Uday Mohan, Suman Sundar Mohanty, Subarna Roy, Vivek Sagar, Deepali Savargaonkar, Babasaheb V. Tandale, Roshan Kamal Topno, C. P. Girish Kumar, R. Sabarinathan, V. Saravana Kumar, T. Karunakaran, Annamma Jose, Provash Sadhukhan, G. S. Toteja, Shanta Dutta, Manoj Murhekar
Summary: A nationally representative survey in India revealed varying proportions of dengue infections with high antibody titers across different geographic regions, with the northern and southern regions showing higher rates. These findings could be valuable for planning clinical management facilities for dengue infections.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Food Science & Technology
Meera Ongmu Bhutia, Namrata Thapa, Jyoti Prakash Tamang
Summary: The microbiological quality of traditionally preserved fish products from Sikkim, India was assessed in this study, revealing the presence of various potential pathogenic bacteria. Antibiotic susceptibility testing showed that the majority of samples were sensitive to several antibiotics. However, enterotoxins produced by Bacillus cereus and Staphylococcus were detected in some samples, highlighting potential health risks associated with these products.
Editorial Material
Hematology
Jose Antonio Diaz
Summary: The study investigated the role of Slc44a2 in thrombosis, highlighting the importance of HNA-3a expression for neutrophil adhesion to VWF mediated by Slc44a2. Specific flow conditions induced the formation of neutrophil extracellular traps, demonstrating the complex mechanisms involved in neutrophil recruitment and activation during thrombosis.
Article
Microbiology
Debaprasad Parai, Subrat Kumar Sahoo, Matrujyoti Pattnaik, Aparajita Swain, Annalisha Peter, Lopamudra Jena Samanta, Rashmita Pradhan, Hari Ram Choudhary, Kanhu Charan Nahak, Sanghamitra Pati, Debdutta Bhattacharya
Summary: This study aimed to assess the prevalence of human brucellosis among community members involved in animal husbandry in Odisha, India. The results showed that the prevalence of brucellosis was associated with sociodemographic factors such as gender, living settings, and household numbers.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Sourav Datta, Manab Ghosh, Moumita Paul, Prantiki Halder, Sudeshna Mallik, Sumi Mukhopadhyay, Bibhuti Saha, Pratip Kumar Kundu
Summary: This study investigates the clinical and biochemical features of dengue fever cases in North 24 Parganas, West Bengal, India, and suggests that CRP may be a marker for dengue severity in the region. The study attempts to estimate a CRP dengue severity cut-off value based on routine immunoturbidometric evaluation from a dengue hyperendemic zone in North 24 Parganas, West Bengal, Eastern India.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Microbiology
Aki Hirabayashi, Hideji Yanagisawa, Hiromizu Takahashi, Koji Yahara, Philipp Boeing, Bethan Wolfenden, Vandarith Nov, Vichet Lorn, Mom Veng, Vuth Ann, Chau Darapheak, Keigo Shibayama, Masato Suzuki
Summary: This study conducted on-site genomic epidemiological analysis of carbapenemase-producing gram-negative bacteria isolated in Cambodia, identifying multiple carbapenemase genes and demonstrating their carriage and dissemination among different bacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Gabrielle Levi, Mor Lurie-Weinberger, Alona Keren-Paz, Antoine O. Andremont, David Schwartz, Yehuda Carmeli
Summary: This paper illustrates the underlying mechanisms of co-colonization of antibiotic-resistant bacteria and discusses the potential consequences. It focuses on horizontal gene transfer and patient-to-patient transmission.
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)