Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Bong-Sung Kim, Shih-Heng Chen, Mauro Vasella, Marco Guidi, Epameinondas Gousopoulos, Nicole Lindenblatt, Huang-Kai Kao
Summary: This study investigates the in vivo fate of mechanically processed stromal vascular fraction (mSVF) for regenerative purposes. By establishing an arteriovenous (AV) shunt model in rats, the study shows that vascularized mSVF can proliferate and express adipocyte-specific markers.
Article
Surgery
Kevin M. Urlaub, Kavitha Ranganathan, Jeremy V. Lynn, Alexandra O. Luby, Lauren N. Patrick, Noah S. Nelson, Alexis Donneys, Steven R. Buchman
Summary: Stromal vascular fraction therapy has shown significant efficacy in enhancing bone healing in irradiated fracture repair, improving union rates and bone vascularization metrics while restoring bone cellularity and maturity despite preoperative irradiation. This study provides a promising foundation for the development of noncultured, cell-based therapies for clinical implementation.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Review
Oncology
Rawan Al-kharboosh, Jonathan Jude Perera, Alexandra Bechtle, Guojun Bu, Alfredo Quinones-Hinojosa
Summary: Neurodegenerative disorders are characterized by the irreversible loss of cognitive functions and CNS structures, and the lack of preventive and regenerative measures has led to traditional symptomatic management as the only available therapeutic recourse. However, recent research suggests that the adipose-derived stromal vascular fraction (SVF) may hold promise as a cellular treatment for neurodegenerative diseases, with improvements seen in clinical applications for conditions such as Multiple Sclerosis, Alzheimer's disease, and Parkinson's disease.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Paul Girard, Joelle Dulong, Jerome Duisit, Camille Mocquard, Simon Le Gallou, Benoit Chaput, Elise Lupon, Eric Watier, Audrey Varin, Karin Tarte, Nicolas Bertheuil
Summary: This study optimized the nanofat grafting technique to increase the yield of adipose-derived stem cells (ASCs) extraction. The results showed that the stromal vascular fraction (SVF) obtained by 20 inter-syringe passages contained more cells, including ASCs, and had better anti-inflammatory properties. This study provides an important foundation for decellularized tissue methods that can provide the required quantity and quality of cells in a clinical setting.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ibrahim Vargel, Ali Tuncel, Nilsu Baysal, Irem Hartuc-Cevik, Feza Korkusuz
Summary: SVF has the potential to modulate inflammation and initiate regeneration in joint tissues, making it a potential approach for treating knee osteoarthritis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
S. Gandolfi, B. Pileyre, L. Drouot, I. Dubus, I. Auquit-Auckbur, J. Martinet
Summary: Muscle regeneration is a complex process that requires precise interaction between immune and muscular cells. The mechanisms underlying autoimmune myopathies are not fully understood, but chronic inflammation and decreased muscle regenerative capacity play a role. Immunosuppressive treatments can be ineffective, and alternative therapies such as stromal vascular fraction show promise but lack a clear understanding of their active components and mechanisms of action.
CELL DEATH DISCOVERY
(2023)
Article
Agriculture, Dairy & Animal Science
Gabriele Scattini, Martina Pellegrini, Giulio Severi, Monica Cagiola, Luisa Pascucci
Summary: Adipose-derived MSCs are commonly isolated by collagenase digestion, but waste tissue fragments contain cells that adhere belatedly to the plastic. In this study, three sets of cells that adhered at different time points were analyzed for their properties, and no significant differences were found between the three subpopulations. This procedure improves MSCs yield and shortens the time required for clinical applications.
Article
Cell & Tissue Engineering
Roman Vuerich, Elena Groppa, Simone Vodret, Nadja Annelies Ruth Ring, Chiara Stocco, Fleur Bossi, Chiara Agostinis, Matteo Cauteruccio, Andrea Colliva, Mohammad Ramadan, Francesca Simoncello, Federica Benvenuti, Anna Agnelli, Franca Dore, Flavia Mazzarol, Massimo Moretti, Alice Paulitti, Silvia Palmisano, Nicolo De Manzini, Mattia Chiesa, Manuel Casaburo, Angela Raucci, Daniela Lorizio, Giulio Pompilio, Roberta Bulla, Giovanni Papa, Serena Zacchigna
Summary: Nonhealing wounds have a negative impact on the quality of life for patients and health systems. Skin substitutes have been used, but have limited efficacy due to inadequate vascularization. The use of the stromal vascular fraction (SVF) from adipose tissue shows promise in overcoming this limitation, but additional research is needed to determine its precise mechanism of action.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Surgery
Zhibin Yang, Shengyang Jin, Yu He, Xinyu Zhang, Xuefeng Han, Facheng Li
Summary: The study aimed to investigate the therapeutic potential of microfat, nanofat, and extracellular matrix/stromal vascular fraction gel (SVF-gel) in skin rejuvenation. Results showed that microfat preserved histologic structure integrity and presented advantages in subcutaneous volumetric restoration and skin quality improvement compared to nanofat and SVF-gel. Clinical application of microfat led to marked improvement in skin wrinkles.
AESTHETIC SURGERY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Stefan Wolf, Jeremy W. Deuel, Maija Hollmen, Gunther Felmerer, Bong-Sung Kim, Mauro Vasella, Lisanne Gruenherz, Pietro Giovanoli, Nicole Lindenblatt, Epameinondas Gousopoulos
Summary: Lipedema is characterized by disproportionate adipose tissue increase in the limbs, with a distinct systemic cytokine profile and metabolic activity compared to healthy adipose tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Fermin Robledo, Lila Gonzalez-Hodar, Pablo Tapia, Ana-Maria Figueroa, Fernando Ezquer, Victor Cortes
Summary: Researchers successfully extracted multipotent cells from the brown adipose tissue of newborn mice, forming adipose spheroids with complex organoid structure and enhanced leptin secretion upon insulin stimulation. These findings have important implications for the potential therapeutic application of adipose tissue-derived stromal vascular fraction cells.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Yuan Cai, Fang Zhang, Jingwei Feng, Bihua Wu, Hai Li, Shune Xiao, Feng Lu, Zairong Wei, Chengliang Deng
Summary: This study found that stromal vascular fraction gel (SVF-gel) can promote the early healing of chronic wounds by secreting various cytokines and promoting cell proliferation and migration ability. Autologous SVF-gel transplantation in 20 patients with chronic wounds resulted in 100% wound closure eventually, with an average healing time of 28.3 days. Long-term follow-up ranging from 2 to 6 years showed no wound recurrence.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Engineering, Biomedical
Isabel Mayoral, Elisa Bevilacqua, Gorka Gomez, Abdelkrim Hmadcha, Ignacio Gonzalez-Loscertales, Esther Reina, Julio Sotelo, Antonia Dominguez, Pedro Perez-Alcantara, Younes Smani, Patricia Gonzalez-Puertas, Ana Mendez, Sergio Uribe, Tarik Smani, Antonio Ordonez, Israel Valverde
Summary: In this study, a patient-specific patch was designed using a hybrid 3D printing technique and VSMC differentiation. Through computational modeling and experimental evaluation, the patch demonstrated favorable hemodynamic characteristics, mechanical properties, cell infiltration, viability, and functionality. This innovative 3D biotechnology holds great potential for applications in regenerative medicine and heart disease prevention.
MATERIALS TODAY BIO
(2022)
Article
Veterinary Sciences
Khan Sharun, Kaveri Jambagi, Rohit Kumar, Mudasir Bashir Gugjoo, Abhijit M. Pawde, Hardeep Singh Tuli, Kuldeep Dhama, Amarpal
Summary: Adipose tissue-derived stromal vascular fraction (AdSVF) is a heterogeneous cell population with therapeutic potential for various conditions, including osteoarthritis, wound healing, and orthopaedic disorders. Compared to in vitro expanded adipose tissue-derived mesenchymal stem cells (AdMSCs), AdSVF has several advantages.
VETERINARY QUARTERLY
(2022)
Article
Cardiac & Cardiovascular Systems
Garrett N. Coyan, Antonio D'Amore, Yasumoto Matsumura, Drake D. Pedersen, Samuel K. Luketich, Vesselin Shanov, William E. Katz, Tirone E. David, William R. Wagner, Vinay Badhwar
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2019)
Article
Biochemistry & Molecular Biology
Antonio D'Amore, Samuel K. Luketich, Richard Hoff, Sang-Ho Ye, William R. Wagner
Article
Engineering, Biomedical
Yasumoto Matsumura, Yang Zhu, Hongbin Jiang, Antonio D'Amore, Samuel K. Luketich, Verena Charwat, Tomo Yoshizumi, Hideyoshi Sato, Brenda Yang, Takafumi Uchibori, Kevin E. Healy, William R. Wagner
Article
Engineering, Biomedical
Prerak Gupta, Katherine L. Lorentz, Darren G. Haskett, Eoghan M. Cunnane, Aneesh K. Ramaswamy, Justin S. Weinbaum, David A. Vorp, Biman B. Mandal
ACTA BIOMATERIALIA
(2020)
Article
Rehabilitation
Nick J. Willett, Michael L. Boninger, Laura J. Miller, Luis Alvarez, Tomoki Aoyama, Marzia Bedoni, Kelley Ann Brix, Carmelo Chisari, George Christ, Christopher L. Dearth, Trevor A. Dyson-Hudson, Christopher H. Evans, Stephen M. Goldman, Kenton Gregory, Alice Gualerzi, Joseph Hart, Akira Ito, Hiroshi Kuroki, M. Terry Loghmani, David L. Mack, Gerard A. Malanga, Linda Noble-Haeusslein, Paul Pasquina, Joseph A. Roche, Lloyd Rose, Martin J. Stoddart, Junichi Tajino, Carmen Terzic, Kimberly S. Topp, William R. Wagner, Stuart J. Warden, Steven L. Wolf, Hua Xie, Thomas A. Rando, Fabrisia Ambrosio
ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION
(2020)
Article
Cardiac & Cardiovascular Systems
Garrett N. Coyan, Lindemberg da Mota Silveira-Filho, Yasumoto Matsumura, Samuel K. Luketich, William Katz, Vinay Badhwar, William R. Wagner, Antonio D'Amore
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2020)
Article
Chemistry, Multidisciplinary
Yingqi Chen, Sang-Ho Ye, Yang Zhu, Xinzhu Gu, Shinichi Higuchi, Guojiang Wan, William R. Wagner
ADVANCED MATERIALS INTERFACES
(2020)
Article
Engineering, Biomedical
A. Adamo, A. Bruno, G. Menallo, M. G. Francipane, M. Fazzari, R. Pirrone, E. Ardizzone, W. R. Wagner, A. D'Amore
Summary: Immunohistochemistry for vascular network analysis plays a fundamental role in basic science, translational research, and clinical practice. However, manual identification of vascularization in histological tissue images is time-consuming and operator-dependent. In this study, an automatic algorithm (BVD) was developed for quantitative analysis of blood vessels in immunohistochemical images. The algorithm demonstrated robustness and versatility in quantifying number, area, and spatial distribution of blood vessels across different datasets.
ANNALS OF BIOMEDICAL ENGINEERING
(2022)
Article
Engineering, Biomedical
Seungil Kim, Kamil W. Nowicki, Bradley A. Gross, William R. Wagner
Summary: Cerebral aneurysms are vascular lesions caused by biomechanical failure of the vessel wall, potentially leading to life-threatening brain hemorrhage. Current treatment options include open surgery and minimally invasive endovascular techniques, with embolization using appropriate materials helping to prevent rupture and subsequent clinical sequelae.
Article
Biochemistry & Molecular Biology
Michael Nguyen-Truong, Seungil Kim, Courtney Doherty, Megan Frederes, Kristen LeBar, Soham Ghosh, Peiman Hematti, Raghavan Chinnadurai, William R. Wagner, Zhijie Wang
Summary: This study assessed the impact of right ventricle (RV) tissue mechanics on mesenchymal stromal cell (MSC) behavior. It was found that MSCs produced the highest levels of vascular endothelial growth factor (VEGF) in the failing, isotropic group, which was greater than the normal, isotropic group (p < 0.05). The presence of anisotropy led to trends of increased VEGF production in normal groups, but this effect was absent in failing groups.
Article
Chemistry, Multidisciplinary
Anthony Mercader, Sang-Ho Ye, Seungil Kim, Ryan A. Orizondo, Sung Kwon Cho, William R. Wagner
Summary: A new hybrid PDMS material with excellent anti-fouling properties was synthesized in this study, which can be widely used in the manufacture of biomedical devices. Experimental results showed that the PDMS-SB hybrid material has lower platelet deposition, and the manufacturing process is comparable to unmodified PDMS.
Article
Engineering, Biomedical
Seungil Kim, Kamil W. Nowicki, Sangho Ye, Kyeongwoo Jang, Moataz Elsisy, Mohamed Ibrahim, Youngjae Chun, Bradley A. Gross, Robert M. Friedlander, William R. Wagner
Summary: A new bioabsorbable cerebral aneurysm embolization device, coated on a biodegradable metallic coil, has been developed. The device has good physical properties, enzymatic degradation profiles, and antioxidant activity, and provides better protection for the underlying metallic coil. In vitro and in vivo experiments have shown that the device has the potential to be a fully bioabsorbable embolization coil.
Article
Engineering, Biomedical
Keishi Kohyama, Hideyoshi Sato, Takafumi Uchibori, Keisuke Takanari, Johnny Huard, Stephen F. Badylak, Antonio D'Amore, William R. Wagner
Summary: The study aims to generate a cellularized, elastic, biocomposite scaffold using electrohydrodynamic processing that can develop and mature as muscle in vivo and be transferred as a thin muscle flap with a vascular and neural pedicle, for the reanimation of facial structures following paralysis.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Seungil Kim, Sang-ho Ye, Arianna Adamo, Ryan A. Orizondo, Jaehyuk Jo, Sung Kwon Cho, William R. Wagner
JOURNAL OF MATERIALS CHEMISTRY B
(2020)