Review
Chemistry, Medicinal
Madhan Jeyaraman, Sathish Muthu, Prakash Gangadaran, Rajni Ranjan, Naveen Jeyaraman, Gollahalli Shivashankar Prajwal, Prabhu Chandra Mishra, Ramya Lakshmi Rajendran, Byeong-Cheol Ahn
Summary: The periosteum, rich in pluripotent stem cells, plays a crucial role in fracture healing and remodeling. While its potential in tissue engineering is recognized, further research is needed to explore the bone-forming capabilities of its stem cells.
Article
Biochemistry & Molecular Biology
Stefano Pulcini, Lucia Merolle, Chiara Marraccini, Eleonora Quartieri, Daniele Mori, Davide Schiroli, Pamela Berni, Barbara Iotti, Erminia Di Bartolomeo, Roberto Baricchi, Roberto Sala, Thelma A. Pertinhez
Summary: This study demonstrated that PRP obtained through apheresis contains high concentrations of growth factors that stimulate proliferation and differentiation of pre-osteocytic cells. The efficacy of PRP in promoting bone growth depends on its concentration, with 25% PRP showing the best effects on cell growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Meadhbh A. Brennan, Mario Barilani, Francesco Rusconi, Julien de Lima, Luciano Vidal, Cristiana Lavazza, Lorenza Lazzari, Rosaria Giordano, Pierre Layrolle
Summary: BMSCs demonstrate superior osteogenic potential in vitro, while CBMSCs show better chondrogenic differentiation and secretion of interleukins. Although BMSCs exhibit higher cell engraftment and ectopic bone formation compared to CBMSCs in vivo, priming CBMSCs with supplements can also induce bone formation.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Dongdong Fei, Yanmin Xia, Qiming Zhai, Yazheng Wang, Feng Zhou, Wanmin Zhao, Xiaoning He, Qintao Wang, Yan Jin, Bei Li
Summary: This study focuses on the clonal osteogenic heterogeneity of periodontal ligament stem cells (PDLSCs) and uncovers a mechanism where exosome-mediated PINK1/Parkin-dependent mitophagy regulates interclonal communication among SCCs with osteogenic heterogeneity, promoting osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Endocrinology & Metabolism
Hengyi Wang, Jianshu Zhu, Yuanliang Xia, Yuehong Li, Changfeng Fu
Summary: With the increasing incidence of spine-related diseases due to demographic changes and lifestyle, it has become a global public health problem, leading to significant economic burden. Current treatment methods for these diseases have limitations, and there is an urgent need to find methods that can limit disease progression or cure diseases at the etiological level. Platelet-rich plasma (PRP) extracted from venous blood contains growth factors that promote stem cell proliferation, angiogenesis, bone regeneration, and tissue regeneration capacity. This review discusses the application of PRP in the treatment of spine-related diseases and its clinical use in spinal surgery.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Pharmacology & Pharmacy
Daniel Garcia-Sanchez, Alberto Gonzalez-Gonzalez, Patricia Garcia-Garcia, Ricardo Reyes, Maria Isabel Perez-Nunez, Jose A. Riancho, Carmen Evora, Jose Carlos Rodriguez-Rey, Flor M. Perez-Campo
Summary: The study demonstrates a method to enhance the osteogenic potential of MSCs by modulating the expression levels of specific genes. It was found that using Locked Nucleic Acid-Antisense Oligonucleotides and a scaffold releasing low doses of BMP-2 can increase the osteogenic potential of MSCs. By specifically targeting the Wnt pathway modulator Sfrp1, the efficiency of MSC bone regeneration can be improved and osteogenic differentiation in osteoporotic patients' MSCs can be induced.
Article
Veterinary Sciences
Uffaq Shabir, Irfan Ahmad Bhat, Bilal Ahmad Pir, Mukesh Kumar Bharti, Sriti Pandey, Sai Kumar Gutulla, Mihir Sarkar, Yasotha Thirupathi, Vikash Chandra, Arvind Sonewane, Taru Sharma Gutulla
Summary: This study investigates the roles of bone morphogenetic protein (BMP) and Notch signaling pathways in the differentiation of canine bone marrow-derived MSCs by silencing specific genes. The results show that Runx2 plays an essential role in directing MSCs towards osteogenic lineage, while Smad-mediated BMP signaling induces osteoblast-specific gene expression and the Notch pathway negatively regulates osteogenic differentiation of canine MSCs.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Cell Biology
Jia Wang, Yue Sun, Yiping Liu, Jize Yu, Xiaolin Sun, Lin Wang, Yanmin Zhou
Summary: This study investigated the existence of MSCs in Schneiderian membrane and the effects of PRF on these cells. The results showed that MSCs existed in Schneiderian membrane and PRF stimulation promoted cell proliferation, migration, and osteogenic differentiation. The application of PRF in maxillary sinus floor elevation could accelerate bone healing and increase the quantity and quality of new bone.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Masami Kanawa, Akira Igarashi, Katsumi Fujimoto, Tania Saskianti, Ayumu Nakashima, Yukihito Higashi, Hidemi Kurihara, Yukio Kato, Takeshi Kawamoto
Summary: MSCs have the potential to differentiate into various mature cell types and are a promising source for regenerative medicine. Marker genes can be identified to predict the osteogenic differentiation potential by comparing different sources of MSCs samples.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Carla Ferreira-Baptista, Andre Queiros, Rita Ferreira, Maria Helena Fernandes, Bruno Colaco, Pedro Sousa Gomes
Summary: Mesenchymal stromal cells (MSCs) from adipose tissue, especially from the falciform ligament (FL-ADSCs), show potential as a source for bone tissue regeneration. This study aimed to characterize the osteogenic functionality of FL-ADSCs compared to control ADSCs from a visceral region. The FL-derived populations expressed higher levels of osteogenic genes and markers, particularly with dexamethasone induction, while control ADSCs were more responsive to retinoic acid. FL-ADSCs were identified as a potential source for bone regenerative applications, and the selection of the most suitable osteogenic induction program is crucial.
BIOENGINEERING-BASEL
(2022)
Article
Engineering, Biomedical
Ming-Chung Wu, Helen Wenshin Yu, Yin-Quan Chen, Meng-Hsin Ou, Ricardo Serrano, Guan-Lin Huang, Yang-Kao Wang, Kung-hui Lin, Yu-Jui Fan, Chi-Chang Wuj, Juan C. del Alamo, Arthur Chiou, Shu Chien, Jean-Cheng Kuo
Summary: Through engineered extracellular matrices, the study reveals the association between cell shape, focal adhesion signals, and mesenchymal stromal cell (MSC) lineage commitment. Square cells form mature focal adhesions and symmetrical radial fiber bundles, while circular cells align their transverse fibers parallel to the cell edge. The contractile force generated by the myosin IIA-enriched transverse fibers is distributed through the symmetrical radial fiber bundles, driving focal adhesion organization and maturation and regulating MSC fate decision through YAP mechanotransduction signaling.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Oliwia Milek, Dino Tur, Lucia Ahcin, Olha Voitseshyna, Christian Behm, Oleh Andrukhov
Summary: This study investigated the impact of osteogenic differentiation on the immunomodulatory properties of hPDL-MSCs, and found that osteogenic differentiation reduced the ability of hPDL-MSCs to suppress CD4(+) T lymphocyte proliferation and enhanced this ability in the presence of IL-1 beta. Additionally, the expression and activity of indoleamine-2,3-dioxygenase and interleukin-8 were also affected by the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Article
Biochemistry & Molecular Biology
Maximilian Fischer, Janosch Schoon, Eric Freund, Lea Miebach, Klaus-Dieter Weltmann, Sander Bekeschus, Georgi I. Wassilew
Summary: Cold physical plasma (CPP) treatment can affect the metabolic activity and cell proliferation of bone marrow mesenchymal stromal cells, and induce changes in mitochondria, nucleus, and cytoskeletal structure. In addition, CPP treatment can promote anti-inflammatory, promatrix-assembling, and osteoclast-regulating effects. This study is important for the development of bone regeneration therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)