Article
Cardiac & Cardiovascular Systems
Youngeun Lee, Ja-Yoon Gu, Hyun Kyung Kim
Summary: Lupus anticoagulant (LA), composed of heterogeneous autoantibodies, is closely associated with thrombotic events. Patients with positive results for both the dilute Russell viper venom time (dRVVT) and silica clotting time (SCT) were found to have a higher risk of developing thrombotic events, along with higher rates of positivity for aCL and aB2GPI antibodies.
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
(2022)
Article
Medical Laboratory Technology
Armando Tripodi, Veena Chantarangkul, Cristina Novembrino, Erica Scalambrino, Massimo Boscolo-Anzoletti, Marigrazia Clerici, Federica Rossi, Flora Peyvandi
Summary: This study demonstrates that by making slight modifications to the OSA for FVIII, it can reliably measure the concentration of emicizumab and assist clinicians in managing patients on emicizumab. Additionally, the use of CA assay with bovine reagents can effectively measure the inhibitors to FVIII in patients receiving emicizumab treatment.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Medical Laboratory Technology
Marija Milos, Domagoj Marijancevic, Ana Kozmar, Branimir Anic, Desiree Coen Herak
Summary: The concomitant presence of lupus anticoagulant (LA) and monoclonal immunoglobulin in the same patient is uncommon. In this case, the patient showed dramatically prolonged prothrombin time and activated partial thromboplastin time without bleeding manifestations. The normalization of coagulation screening results with decreasing monoclonal IgM concentration suggests a potential relationship between monoclonal IgM and the prolonged coagulation results.
CLINICA CHIMICA ACTA
(2022)
Review
Biochemistry & Molecular Biology
Armando Tripodi
Summary: Lupus anticoagulant (LA) is a crucial laboratory parameter for the diagnosis and management of antiphospholipid syndrome (APS). Proper detection of LA is essential for long-term treatment of patients with thrombotic events, requiring adherence to guidelines and participation in quality assessment schemes.
Article
Veterinary Sciences
Juan A. De Pablo-Moreno, Antonio Liras, Luis Revuelta
Summary: This study aimed to provide an accurate determination and standardization of factor V levels, prothrombin time and activated partial thromboplastin time in Mus musculus. A mouse-based protocol was designed and evaluated in 66 healthy animals using a semi-automated coagulometer and human diagnostic reagents. The most stable period for blood sample extraction was found to be the first 6 h of light. Reference intervals were established for factor V, prothrombin time, and activated partial thromboplastin time. The results have implications for biomedical research and transfusional medicine.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Subramanian Senthilkumaran, Sasikumar Sampath, Stephen W. Miller, Jose R. Almeida, Jarred Williams, Harry F. Williams, Ponniah Thirumalaikolundusubramanian, Ketan Patel, Sakthivel Vaiyapuri
Summary: Snakebite envenoming is a common and serious issue in rural tropical communities. Russell's viper is a medically important snake species in India, causing a wide range of pathological conditions. This case report describes a rare complication of partial segmental thrombosis in the corpus cavernosum following snakebite, and discusses its diagnosis and treatment.
Article
Hematology
Christoph Schriefl, Christian Schoergenhofer, Juergen Grafeneder, Michael Poppe, Christian Clodi, Matthias Mueller, Florian Ettl, Bernd Jilma, Pia Wallmueller, Nina Buchtele, Constantin Weikert, Heidrun Losert, Michael Holzer, Fritz Sterz, Michael Schwameis
Summary: Coagulation abnormalities following successful resuscitation from cardiac arrest may be linked to unfavorable neurologic outcomes. Specifically, prolonged aPTT upon admission is associated with an increased risk of unfavorable neurologic function after successful resuscitation.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Pharmacology & Pharmacy
Navodipa Bhattacharya, Nivedita Kolvekar, Sukanta Mondal, Angshuman Sarkar, Dibakar Chakrabarty
Summary: Vipegrin, a 6.8 kDa Kunitz-type serine proteinase inhibitor, has been purified from Russell's viper venom. It inhibits the catalytic activity of trypsin and shows disintegrin-like properties, inhibiting collagen and ADP-induced platelet aggregation. Vipegrin is cytotoxic to MCF7 breast cancer cells and disrupts their adhesion and invasive abilities.
Article
Medical Laboratory Technology
Josep Miquel Bauca, Eva Ajzner, Janne Cadamuro, Andreas Hillarp, Ann Helen Kristoffersen, Piet Meijer
Summary: This study assessed the impact of methodological diversity on APTT mixing test results and their interpretation. Despite using different methodologies, most European laboratories obtained valid results. However, the classification of the results could be improved.
CLINICA CHIMICA ACTA
(2022)
Article
Medicine, General & Internal
Wan Hui Wong, Chuen Wen Tan, Nabeelah Binti Abdul Khalid, Nadjwa Zamalek Dalimoenthe, Christina Yip, Chaicharoen Tantanate, Rodelio D. Lim, Ji Hyun Kim, Heng Joo Ng
Summary: Reagent type significantly affects APTT-based clot waveform analysis (CWA), and standardized methods are needed for potential diagnostic use. A study involving 1055 healthy volunteers from the Asia-Pacific region showed that CWA parameters varied less than 10% between reagent lots. Age and gender were found to have a weak correlation with CWA, requiring further investigation.
Article
Medicine, General & Internal
Yutaka Tsukamoto, Takashi Sugimoto, Masataka Umeda, Takeharu Kato, Yukari Hiura, Kouichi Morita, Koya Ariyoshi
Summary: Severe fever with thrombocytopenia syndrome (SFTS) patients often have a prolonged activated partial thromboplastin time (APTT), which is a risk factor for severe disease. The exact mechanism behind this prolonged APTT is not well understood. While the presence of antiphospholipid (aPL) antibodies has been documented in viral infections, it has never been reported in SFTS patients. Here, we report the first SFTS patient with both prolonged APTT and transiently positive aPL antibodies (lupus anticoagulants and anticardiolipin antibodies) without any coagulation factor deficiency.
Article
Hematology
Osamu Kumano, Jean Amiral, Claire Dunois, Marie Peyrafitte, Gary W. Moore
Summary: The study investigated the influence of direct factor Xa inhibitors on clotting time tests and established an ICA matrix to differentiate between different sample types, achieving high specificity and lower sensitivity for DiXaI samples compared to other devices.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Suveena Sukumaran, Vinod Manoharan Prasanna, Laladhas Krishna Panicker, Achuthsankar S. Nair, Oommen V. Oommen
Summary: In this study, potential inhibitors of snake venom PLA(2) were identified through pharmacophore-based virtual screening, docking, and dynamics approach. The best pharmacophore model consisted of one hydrogen bond acceptor, two hydrogen bond donors, and one hydrophobic region. CAP04815700 was found to have similar inhibitory potential as the crystal ligand, and further investigation of its bioactivity will be conducted in vitro and in vivo.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Hematology
Gary W. Moore, Eleanor Foxton, Sean Platton, Nada Yartey, Danielle White, Stephen G. MacDonald
Summary: This study investigated the frequency of single LA assay positivity in triple-positive patients and found that a significant proportion of patients may be positive in one of the assays, posing a significant risk for misdiagnosis and inappropriate management through single LA assay repertoires.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Steven Andrew Baker, Jing Jin, Christopher Pfaffroth, Trang Vu, James L. Zehnder
Summary: DOAC-Stop effectively reduces DOAC interference in LAC testing. It has no impact on LAC testing in the absence of DOAC therapy and allows for the identification of all LAC positive and negative controls. DOAC-Stop removes false positives and false negatives caused by DOAC interference.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Arne Vandevelde, Walid Chayoua, Bas de Laat, Jean-Christophe Gris, Gary W. Moore, Jacek Musial, Stephane Zuily, Denis Wahl, Katrien M. J. Devreese
Summary: The study evaluated the traditional 40/80-unit thresholds used for categorization of antiphospholipid antibodies in different analytical systems, and found that alternative thresholds can improve consistency in interpretation. By using different thresholds, the positivity levels of antiphospholipid antibodies can be more accurately assessed.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Pauline Vermeiren, Arne Vandevelde, Harlinde Peperstraete, Katrien M. J. Devreese
Summary: This study compared the effectiveness of global coagulation assays, including thrombin generation assay (TGA), with the anti-Xa assay for monitoring heparin therapy. The results showed that the TGA may not be sensitive enough for low concentrations of low molecular weight heparin (LMWH), but it could be a useful additional tool for monitoring heparin therapy in heparin-resistant patients.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2022)
Article
Hematology
Gualtiero Palareti, Angelo A. Bignamini, Tomasz Urbanek, Michela Cini, Young-Jun Li, Juraj Madaric, Kamel Bouslama, German Y. Sokurenko, Giuseppe M. Andreozzi, Jiri Matuska, Armando Mansilha, Victor Barinov
Summary: This study investigated the influence of clinical presentation, extent of venous thrombosis, and presence of residual vein obstruction on the decision about the duration of secondary prophylaxis after a first venous thromboembolism. The results showed that the decision varied among countries, with most patients with proximal or associated deep vein thrombosis continuing treatment, while patients with isolated distal thrombosis were more likely to stop treatment. Factors such as presence of concomitant diseases and risk of post-thrombotic syndrome also influenced the decision.
THROMBOSIS RESEARCH
(2022)
Article
Hematology
Thomas Foret, Virginie Dufrost, Marie Heymonet, Jessie Risse, Gilbert C. Faure, Huguette Louis, Jeremy Lagrange, Patrick Lacolley, Katrien Devreese, Sebastien Gibot, Veronique Regnault, Stephane Zuily, Denis Wahl
Summary: This study demonstrated that the levels of circulating endothelial cells (CECs) were associated with thromboembolic events in patients with antiphospholipid antibody (aPL) and/or autoimmune diseases.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Veterinary Sciences
Despoina Georgaki, Femke Vandael, Helena Cardoso de Carvalho Ferreira, Maria Eleni Filippitzi, Patrick De Backer, Mathias Devreese, Jeroen Dewulf, Siska Croubels
Summary: This study conducted a qualitative risk assessment to estimate the risks associated with reduced homogeneity and stability, as well as increased residual concentrations of antimicrobial drugs in medicated feed and drinking water on pig farms. Factors such as human error during the preparation of medicated feed and dosing pump type for medicated drinking water were found to pose moderate to high risks. It is crucial to identify and understand these factors in order to ensure the effectiveness and safety of antimicrobial drug treatment.
BMC VETERINARY RESEARCH
(2023)
Letter
Anesthesiology
Michael Vandenheuvel, Korneel Vandewiele, Carla Van Gompel, Pieter M. De Kesel, Piet Wyffels, Filip De Somer, Katrien M. J. Devreese, Patrick F. Wouters
EUROPEAN JOURNAL OF ANAESTHESIOLOGY
(2023)
Article
Peripheral Vascular Disease
Gualtiero Palareti, Victor Barinov, Tomasz Urbanek, Michela Cini, Young-Jun LI, Kamel Bouslama, Jiri Matuska, Armando Mansilha, Juraj Madaric, German Y. Sokurenko, Giuseppe M. Andreozzi, WHITE Study Grp
Summary: This study investigated the complications occurring during the follow-up of VTE patients who received treatment decisions from clinical centers. The use of DOACs for extended treatments was found to be the most common. Overall, the rate of primary events during follow-up was low.
INTERNATIONAL ANGIOLOGY
(2023)
Article
Hematology
Elvira Grandone, Emilia Antonucci, Donatella Colaizzo, Antonio De Laurenzo, Benilde Cosmi, Michela Cini, Cristina Legnani, Sophie Testa, Maurizio Margaglione, Gualtiero Palareti
Summary: This study investigated the impact of risk factors on pregnancy- or combined oral contraceptive (COC)-associated venous thromboembolism (VTE). Results showed that family history was a significant and independent risk factor for COC-VTE and pregnancy-associated VTE. Therefore, family history plays a crucial role in predicting the occurrence of VTE in the context of pregnancy or COC use.
THROMBOSIS AND HAEMOSTASIS
(2023)
Review
Cardiac & Cardiovascular Systems
Rachel Gehlen, Arne Vandevelde, Bas de Laat, Katrien M. J. Devreese
Summary: This systematic review examined if the thrombin generation (TG) assay could be useful for the diagnosis and risk assessment of antiphospholipid syndrome (APS). The findings suggest that APS patients have increased aPC resistance compared to healthy and thrombotic controls, but its diagnostic and prognostic value compared to current strategies is unclear. More research is needed to determine the potential added value of other TG parameters in APS diagnosis.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Medicine, General & Internal
Benilde Cosmi, Cristina Legnani, Michela Cini, Laura Borgese, Michelangelo Sartori, Gualtiero Palareti
Summary: Heparin-induced thrombocytopenia (HIT) is a rare immuno-mediated adverse reaction with high thrombotic and mortality risk. A retrospective study was conducted to evaluate the incidence and outcomes of HIT cases diagnosed at a tertiary care hospital from 2007 to 2018. The HIT incidence was 0.16/1000 patient/years in surgical patients and 0.15/1000 patient/years in medical patients.
INTERNAL AND EMERGENCY MEDICINE
(2023)
Meeting Abstract
Hematology
Katrien Devreese
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Meeting Abstract
Hematology
Arne Vandevelde, Luna Crevits, Katrien M. J. Devreese
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Meeting Abstract
Hematology
Pauline Vermeiren, Arne Vandevelde, Katrien M. J. Devreese
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Meeting Abstract
Hematology
Delphine De Smet, Dimitri Hemelsoet, Katrien Devreese
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Article
Hematology
Arne Vandevelde, Jean-Christophe Gris, Gary W. Moore, Jacek Musial, Stephane Zuily, Denis Wahl, Katrien M. J. Devreese
Summary: Currently, the added value of antiphosphatidylserine/prothrombin antibodies testing in the diagnosis of obstetric antiphospholipid syndrome seems limited compared with other antibodies. However, it may be useful in specific subsets of patients.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)