Review
Neurosciences
Jenny I. Szu, Devin K. Binder
Summary: This review evaluates the role of AQP4 dysregulation and mislocalization in epilepsy, highlighting their significant impact on neuronal hyperexcitability. Research has shown that the mislocalization of AQP4 can greatly affect the excitability of neurons, playing a crucial role in the development of epilepsy.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yilin Zheng, Mengyu Li, Binman Liu, Yafei Qin, Jinhua Li, Yu Pan, Xingguo Zhang
Summary: Phytochelatins (PCs) are low molecular-weight polypeptides in plants that can bind to metal ions and affect physiological metabolism. This study found that the expression of PCL in transgenic plants improves resistance to Cd and antioxidant abilities, but also leads to higher levels of Cd accumulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yongyu Zhao, Dongxu Yang, Yumei Liu, Fengqing Han, Zhansheng Li
Summary: In this study, the effects of different factors on the genetic transformation efficiency of broccoli were explored. Hypocotyl explants were found to be more suitable for transgene and gene editing. The usage of hygromycin B at a concentration of 5 mg/L was more advantageous for selecting resistant broccoli sprouts, and genotype 19B42 exhibited the highest transformation rate. Additionally, subcellular localization confirmed the expression of glucoraphanin metabolism-related gene Bol031350 and clubroot resistance gene CRa in the cytoplasm and nucleus. These findings contribute to the improvement of genetic transformation and molecular breeding in Brassica oleracea crops.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Summary: Real-time quantitative PCR is a technique for measuring the content of a specific nucleic acid sequence in a sample, and it can be divided into absolute and relative quantitative methods. Relative quantification is mainly used for gene expression in functional genomics and transcriptome studies. However, to accurately use this technology, key points such as designing specific primers, selecting appropriate reference genes, and performing scientific calculations and statistics are crucial. This article proposes a workflow for relative quantitative PCR and introduces relevant points to help beginners better understand and utilize this technology.
Article
Chemistry, Applied
Tingting Li, Jishi Wang, Zhiying Wang, Lu Qiao, Rui Liu, Shanshan Li, Ailiang Chen
Summary: Mitochondrial genes are commonly used for PCR-based meat adulteration authentication, but variations in gene copies can lead to quantification errors. This study developed a quantitative method using single-copy nuclear genes to accurately identify mutton adulteration, achieving high recovery rates for simulated mutton-pork, mutton-chicken, and mutton-duck samples.
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Chang-Hun Choi, Eiseul Kim, Seung-Min Yang, Da-Som Kim, Seung-Man Suh, Ga-Young Lee, Hae-Yeong Kim
Summary: This study evaluated the applicability of a ddPCR assay for detecting lactic acid bacteria and compared its performance characteristics to those of a qPCR. The results showed that ddPCR is more sensitive than qPCR, but it has limitations in quantifying high bacterial concentrations.
Article
Pathology
Shu Jin Tan, Todd Matthew Pryce, Erin Judith Haygarth, Peter Alan Boan
Summary: The study demonstrated the utility of a Pneumocystis jirovecii quantitative polymerase chain reaction (qPCR) in distinguishing disease from colonization, with varying sensitivity and specificity in HIV and non-HIV patients. Standardization of methods and definitions is necessary for improving generalizability of results.
Article
Biochemistry & Molecular Biology
Zhaohui Wang, Hongyu Jia, Hansong Yu, Tong Wang, Bin Yang
Summary: The genetic characteristics of the genetically modified soybean lectin gene were analyzed using a method based on fluorescence quantitative PCR, revealing a complex functional mechanism that may participate in various stress-related regulatory or signal transduction pathways in different ways. The study identified tissue-specific expression patterns for Lectin2.1 and Lectin2.2, highlighting their similarities in nucleic acid and amino acid composition as well as subcellular localization characteristics.
BIOCHEMICAL GENETICS
(2021)
Article
Microbiology
Bingqian Zhou, Guangpu Yang, Zhe Hu, Kewei Chen, Wei Guo, Xiaojun Wang, Cheng Du
Summary: A new qPCR method was developed to detect T. haneyi infection in horses, showing high specificity and sensitivity. Its accuracy was further validated by comparison with the traditional nested PCR method.
Article
Horticulture
Chenjie Zhang, Chengwei Song, Linfeng Chen, Huili Ma, Yabing Zhang, Dalong Guo, Lili Guo, Xiaogai Hou
Summary: The aim of this study was to identify suitable reference genes for normalizing the expression levels of miRNA in different developmental stages and tissues. Five algorithms were used for stability analysis, and the results identified mtr-MIR160b-p3 and gma-miR394a-5p as the most stable miRNAs during bud development in early-flowering tree peony 'Feng dan', while PC-5p-19095 was the most stable during bud development in late-flowering tree peony 'Lian he'. Additionally, mtr-miR159a was found to be the most stable miRNA during flower development stages of different tree peony varieties.
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Biochemical Research Methods
Zheng Xiao, Ruipeng Fu, Jiyuan Li, Zhengqi Fan, Hengfu Yin
PLANT MOLECULAR BIOLOGY REPORTER
(2016)
Article
Plant Sciences
Zheng Xiao, Xiaobo Sun, Xiaoqing Liu, Chang Li, Lisi He, Shangping Chen, Jiale Su
FRONTIERS IN PLANT SCIENCE
(2016)
Article
Biochemistry & Molecular Biology
Xiao-Bo Sun, Hong-Xiang Ma, Xin-Ping Jia, Yu Chen, Xiao-Qing Ye
Article
Multidisciplinary Sciences
Yanming Deng, Xiaobo Sun, Chunsun Gu, Xinping Jia, Lijian Liang, Jiale Su
Article
Chemistry, Applied
Ruipeng Fu, Zheng Xiao, Zhongli Pan, Hua Wang
FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL
(2019)