Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 139, Issue 41, Pages 14396-14398Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.7b06597
Keywords
-
Categories
Funding
- National Science Foundation Graduate Research Fellowship
- NIH [R01 GM044783, P41 GM103399, T32 GM007215]
Ask authors/readers for more resources
Cloaking its carboxyl groups with a hydrophobic moiety is shown to enable a protein to enter the cytosol of a mammalian cell. Diazo compounds derived from (p-methylphenyl)glycine were screened for the ability to esterify the green fluorescent protein (GFP) in an aqueous environment. Esterification of GFP with 2-diazo-2-(p-methylpheny1)-N,N-clirnethylacetarnide was efficient. The esterified protein entered the cytosol by traversing the plasma membrane directly, like a small-molecule prodrug. As with prodrugs, the nascent esters are substrates for endogenous esterases, which regenerate native protein. Thus, esterification could provide a general means to deliver native proteins to the cytosol.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available