Article
Fisheries
Juan-Ting Liu, John S. Lumsden
Summary: Autophagy and restricted diets like deoxynivalenol can reduce mortality of fish due to bacterial diseases, though their impact on viral diseases is less certain. After infection with viral haemorrhagic septicaemia virus IVb, chloroquine-fed fish showed significantly lower survival rates compared to deoxynivalenol-fed fish, but no treatment significantly altered survival rates compared to controls.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Yin Weili, Huang Wei, Liu Xiaojing, Ni Yangfan, Yang Bai, Shi Qing, Zhao Weiduo, Sun Tao
Summary: The newly developed recombinase polymerase amplification (RPA) assay for the rapid detection of viral hemorrhagic septicemia virus (VHSV) is fast, simple, specific, and sensitive, with a detection limit of 8.3 copies/μL. It has been successfully validated on a large number of fish samples, showing consistent results with traditional RT-PCR and meeting requirements for aquatic animal disease detection.
Article
Biotechnology & Applied Microbiology
Jun Soung Kwak, Ki Hong Kim
Summary: The study showed that attenuating virus growth can be achieved by inserting specific amiR sequences into the viral genome, and enhancing replication of attenuated virus can be achieved by knocking out the Dicer gene in host cells. This suggests that regulating both viral and host cell genes can impact the virus infectivity.
MARINE BIOTECHNOLOGY
(2021)
Article
Fisheries
Amaro Saco, Magali Rey-Campos, Beatriz Novoa, Antonio Figueras
Summary: In this study, mussels were challenged with viral haemorrhagic septicaemia virus (VHSV), and the viral load was eliminated after 24 hours. Mussels showed antiviral activity towards VHSV, indicating that the virus was recognized and eliminated by the mussel immune system, confirming that mussels are not VHSV vectors in the marine environment. The study also revealed the involvement of cytoplasmic viral sensors, JAK-STAT pathway activation, and various downstream antiviral effectors in the mussel immune response.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
S. Puente-Marin, D. Cazorla, V. Chico, J. Coll, M. Ortega-Villaizan
Summary: Recent studies have shown that red blood cells (RBCs) play an important role in fish immune response to viral infections and DNA vaccines. Surface-displaying recombinant bacterins, known as spinycterins, have been found to enhance fish immune response and can be used as a safe alternative method for viral vaccination in aquaculture. In this study, the innate immune response of rainbow trout RBCs, head kidney, and spleen to spinycterins expressing a fragment of the glycoprotein G of viral hemorrhagic septicemia virus (VHSV) was evaluated. The results showed that Nmistic+frg16252-450 spinycterins increased the attachment to RBCs and modulated the expression of interferon and antigen presentation-related genes, while frg16252-450 spinycterins upregulated these genes in the head kidney and spleen. These findings highlight the importance of RBCs in the fish innate immune response to antiviral prophylactics.
Article
Fisheries
Yuyu Sun, Chaonan Xu, Hao Wang, Guo Qiao, Zhenhui Wang, Zheng Li, Qiang Li, Chang Wei
Summary: An attenuated strain of CyHV-2 G-RP7 was obtained by subculture on RyuF-2 cells and GiCF cells. Gibel carp showed no clinical symptoms when immersed or injected intraperitoneally with G-RP7 strain. The protection rates of G-RP7 by immersion and intraperitoneal injection were 92% and 100%, respectively.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Myung-Hwa Jung, Sung-Ju Jung, Taeho Kim
Summary: This study determined the safe concentrations of orally administered saponin and chitosan and demonstrated the efficacy and safety of saponin and chitosan-based vaccines with inactivated viral haemorrhagic septicaemia virus (VHSV). Additional immunisation with a combination of saponin, chitosan, and IV enhanced protection against VHSV.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Peng Chen, Mingming Zhang, Yichan Zhang, Jun Li, Xihe Wan, Tingli Lv, Yiyue Chen, Zhigang Zhao, Zhihao Ma, Zhu Zhu, Lihua Chen, Zhen Li, Zisheng Wang, Guo Qiao
Summary: This study analyzed the impact of Cyprinid herpesvirus 2 (CyHV-2) infection on the gut microbiota and metabolite composition of gibel carp. The results showed that CyHV-2 infection significantly altered the gut microbiota and metabolite profiles, leading to an increase in the opportunistic pathogen Aeromonas. Furthermore, modulation of tryptophan metabolism affected the abundance of Aeromonas, the transcription of antiviral cytokines, and the infection level of CyHV-2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Arathi Dharmaratnam, Arun Sudhagar, Sweta Das, Reshma Rajeev Nair, Sundar Raj Nithianantham, Prasannan Geetha Preena, Narendrakumar Lekshmi, Thangaraj Raja Swaminathan
Summary: This study focused on developing an inactivated vaccine for the goldfish hematopoietic necrosis viral disease (GHNVD) and examining its immunogenicity. The results showed that the formalin-inactivated vaccine triggered immune gene expression in goldfish and significantly improved survival rates in a virus challenge experiment.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Se-Young Cho, Seok Ryel Kim, Bipin Vaidya, Joseph Kwon, Duwoon Kim
Summary: This study highlights that defective GLUT4 translocation-dependent glucose uptake increases the mortality of VHSV-infected olive flounders by inhibiting MPO activity.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Virology
Irene Cano, Eduarda M. Santos, Karen Moore, Audrey Farbos, Ronny van Aerle
Summary: The study revealed that inoculation with non-pathogenic VHSV led to differential gene expression related to immune response and cytokine-mediated signaling pathway, while inoculation with pathogenic VHSV resulted in differential expression of pathways involving lipid metabolism and cytoskeleton rearrangement. The data also suggested a delay in immune response genes and viral sensing in cells inoculated with pathogenic VHSV, indicating transcriptional shutoff and immune avoidance as critical mechanisms of pathogenicity.
Article
Fisheries
Chang Wei, Chaonan Xu, Yuyu Sun, Jinzhou Li, Motohiko Sano, Qiang Li
Summary: This study found that CyHV-2 can establish persistent or latent infection in asymptomatic farmed gibel carp, and different viral mRNA expression patterns were observed in different individuals. This indicates that apparently healthy gibel carp can also be a source of infection.
Article
Fisheries
Hyoung Jun Kim, Jeong Su Park, Se Ryun Kwon, Youngjin Park
Summary: This study aimed to identify immune-associated genes contributing to the progression of VHSV infection in the kidneys of olive flounder. The analysis revealed that VHSV infection broadly affects host metabolism and innate immune responses in the fish.
Article
Veterinary Sciences
Takafumi Ito, Tohru Mekata, Niels Jorgen Olesen, Niels Lorenzen
Summary: The monoclonal antibody IP5B11 used for diagnosing viral haemorrhagic septicaemia (VHS) in fish reacts with all genotypes of VHS virus (VHSV) and also with the carpione rhabdovirus (CarRV). By sequencing the CarRV genome and comparing N protein sequences with five types of fish novirhabdoviruses, the epitope recognized by IP5B11 was identified. Dot blot analysis confirmed that the epitope of IP5B11 is located between amino acids N219 and N233 of the N protein of VHSV. Phylogenetic analysis revealed that CarRV is a new member of the fish novirhabdoviruses.
VETERINARY RESEARCH
(2023)
Article
Fisheries
Cuneyt Tamer, Jessica Benkaroun, Hanne Nur Kurucay, Harun Albayrak, Manfred Weidmann
Summary: Fish viral diseases cause significant economic losses in aquaculture. Viral haemorrhagic septicemia virus (VHSV) is one of the most important viruses affecting fish species. Early detection of diseases is critical for disease prevention and control. This study developed a sensitive RPA assay for detecting VHSV, which showed similar sensitivity to real-time RT-PCR.
JOURNAL OF FISH DISEASES
(2022)
Article
Environmental Sciences
Emily R. Cornwell, Gregory B. Anderson, Destiny Coleman, Rodman G. Getchell, Geoffrey H. Groocock, Janet V. Warg, Angela M. Cruz, James W. Casey, Mark B. Bain, Paul R. Bowser
JOURNAL OF GREAT LAKES RESEARCH
(2015)
Article
Fisheries
Kate E. Breyer, Rodman G. Getchell, Emily R. Cornwell, Gregory A. Wooster, H. George Ketola, Paul R. Bowser
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2015)
Correction
Fisheries
K. E. Breyer, R. G. Getchell, E. R. Cornwell, Andg. A. Wooster
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2015)
Article
Veterinary Sciences
Susan B. Fogelson, Barbara D. Petty, Stephen R. Reichley, Cynthia Ware, Paul R. Bowser, Marcus J. Crim, Rodman G. Getchell, Kelly L. Sams, Helene Marquis, Matt J. Griffin
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2016)
Review
Biology
Colleen A. Burge, Carolyn S. Friedman, Rodman Getchell, Marcia House, Kevin D. Lafferty, Laura D. Mydlarz, Katherine C. Prager, Kathryn P. Sutherland, Tristan Renault, Ikunari Kiryu, Rebecca Vega-Thurber
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2016)
Article
Fisheries
Rodman G. Getchell, Paul R. Bowser, Emily R. Cornwell, Todd Pavek, Philippe Baneux, Drew Kirby, Kelly L. Sams, Helene Marquis
JOURNAL OF AQUATIC ANIMAL HEALTH
(2017)
Article
Environmental Sciences
L. L. Coffee, R. G. Getchell, G. H. Groocock, E. R. Cornwell, G. A. Wooster, J. S. Lumsden, P. R. Bowser
JOURNAL OF GREAT LAKES RESEARCH
(2017)
Article
Fisheries
Rodman G. Getchell, Geoffrey H. Groocock, Emily R. Cornwell, Vanessa L. Schumacher, Lindsay I. Glasner, Barry J. Baker, Stephen A. Frattini, Gregory A. Wooster, Paul R. Bowser
JOURNAL OF AQUATIC ANIMAL HEALTH
(2014)
Article
Fisheries
Lucy Chou, Matt J. Griffin, Trellor Fraites, Cynthia Ware, Hugh Ferguson, Natalie Keirstead, John Brake, Judy Wiles, John P. Hawke, Michael T. Kearney, Rodman G. Getchell, Patricia Gaunt, Esteban Soto
JOURNAL OF AQUATIC ANIMAL HEALTH
(2014)
Article
Environmental Sciences
Emily R. Cornwell, Alex Primus, Po Ting Wong, Gregory B. Anderson, Tarin M. Thompson, Gael Kurath, Geoffrey H. Groocock, Mark B. Bain, Paul R. Bowser, Rodman G. Getchell
JOURNAL OF GREAT LAKES RESEARCH
(2014)
Article
Environmental Sciences
Sierra M. Imanse, Emily R. Cornwell, Rodman G. Getchell, Gael Kurath, Paul R. Bowser
JOURNAL OF GREAT LAKES RESEARCH
(2014)
Article
Virology
Bethany F. Balmer, Rodman G. Getchell, Rachel L. Powers, Jihye Lee, Tinghu Zhang, Michael E. Jung, Maureen K. Pureell, Kevin Snekvik, Hector C. Aguilar
Article
Fisheries
Rodman G. Getchell, Erika J. First, Steven M. Bogdanowicz, Jose A. Andres, Adam T. Schulman, Jordan Kramer, Geofrey E. Eckerlin, John M. Farrell, Helene Marquis
JOURNAL OF FISH DISEASES
(2019)
Article
Fisheries
Rodman G. Getchell, Emily R. Cornwell, Steven Bogdanowicz, Jose Andres, William N. Batts, Gael Kurath, Rachel Breyta, Joanna G. Choi, John M. Farrell, Paul R. Bowser
DISEASES OF AQUATIC ORGANISMS
(2017)