Journal
JOURNAL OF RADIATION RESEARCH
Volume 58, Issue 4, Pages 412-420Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jrr/rrw084
Keywords
IL-6; radioresistance; ROS; mitochondria
Funding
- Ministry of Education, Culture, Sports, Science, and Technology in Japan [26461863]
- Grants-in-Aid for Scientific Research [26461863] Funding Source: KAKEN
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Interleukin (IL)-6 is a multifunctional cytokine and is one of the radiation-induced bystander factors. This study aimed to clarify the mechanism of acquisition of radioresistance through the control of reactive oxygen species (ROS) by IL-6. We used a rat glioma cell line (C6) as tumor cells and a rat astrocyte cell line (RNB) as non-tumor cells. Our results showed that the surviving fraction of C6 cells after 6 Gy irradiation was increased by the addition of IL-6, but that this was not the case in RNB cells. In addition, the number of 53BP1 foci in C6 cells at 30 min after gamma-irradiation were decreased by IL-6. Levels of ROS in whole C6 cells, and superoxide in the mitochondria of C6 cells immediately after gamma-irradiation, were reduced by IL-6, but this was not observed in RNB cells. The mitochondrial membrane potential detected by JC-1 in C6 and RNB cells was inhibited by IL-6 alone. Therefore, it was concluded that IL-6 leads specifically to radioresistance in tumor cells by inhibition of increases in ROS after gamma-irradiation.
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